백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

장력제어 기능을 갖는 환편기 제어 시스템의 설계

여희주(Yeo Hee ?Joo),김재원(Kim Jae Won),김병호(Kim Byoung Ho) 제어로봇시스템학회 2001 제어로봇시스템학회 국제학술대회 논문집 Vol.2001 No.10

고분자 이미다졸-금속 착제 LB막의 선책적 기체 투과성

김병주,이범종 인제대학교기초과학연구소 1999 자연과학 Vol.3 No.-

이미다졸-금속 이온구조를 갖는 고분자 Langmuir-Blodgett(LB)막을 다공성 막 필터 위에 제조하여 산소와 질소의 투과성을 이들의 캐스트 막과 비교하여 검토하였다. 양친매성 고분자 폴리(N-(2-(4-이미다졸일)에틸)말레이미드·alt-1-옥타데센)(IM-O)는 폴리(무수말레산-alt-1-옥타데센)과 히스타민의 반응으로 합성되었다. IM-O는 순수한 물 또는 금속 이온 (철(Ⅲ)과 코발트(Ⅱ)이온)하층액 위에서의 40 mN/m의 표면압력에서도 안정한 일분자막을 유지하였다. 이 일분자막은 30 mN/m의 표면 압력에서 FP-010 막 필터 위에 Y형태로 누적되었다. 이 다중막의 구조는 FT-IR을 통하여 확인하였다. LB막의 균일성과 안정성은 SEM 관찰을 통하여 간접적으로 평가하였고, 산소와 질소의 투과도는 감압법을 이용하여 측정하였다. 본 LB막들의 기체 선택성은 산소보다 질소가 약간 높게 나타났으며, 두 기체에 대하여 모두 고투과성을 나타냈다. 이들 고분자-금속 착체의 캐스트 막을 제조하여 기체 투과도를 측정한 결과 질소에 대한 선택성은 변함이 없었다. The permeability of oxygen and nitrogen was investigated from the polymeric LB films containing imidazole-metal ion complexes and compared with its corresponding cast films on porous membrane filters. The amphiphilic polymer, poly(N-(2-(4-imidazolyl)ethyl)-maleimide-alt-1-octadecene)(IM-O), was synthesized by reaction of poly(maleic anhydride-alt-1-octadecene)with histamine. Chloroform solutions of IM-O were spread on pure water and aqueous metal ions(iron(Ⅲ) and cobalt(Ⅱ)ions) and the monolayers showed high stability up to 40 mN/m against surface pressure. The monolyers of polyion-complexed polymers at the air-water interface can be transferred onto solid substrates such as a porous fluorocarbon membrane filter(FP-010) and a CaF2 plate. The Y-type deposition was found at a surface pressure of 30 mN/m. The molecular structures of LB films were investigated by means of FT-IR spectroscopy. The mechanical stability and uniformity of the LB films on FP-010 membranes were indirectly evidenced by SEM observation of the surface morphology of FP-010 membranes were indirectly evidenced by SEM observation of the surface morphology of FP-010 covered with the LB films. The permeability of oxygen and nitrogen was measured with the pressure method. The LB films showed more or less higher selectivity toward nitrogen, and high permeability was found to both the oxygen and nitrogen. The cast films of the corresponding polymer-metal complexes also showed preference to nitrogen in selectivity.

국부의치용 금속인 Ni-Cr alloy와 Co-Cr alloy의 주조후 냉각방법에 따른 표면조직과 경도의 비교 연구

김재도,김병식,김주원 대구산업정보대학 2002 논문집 Vol.16 No.-

The purpose of this study was to investigate the relationship between the micro-structure and hardness of Ni-Cr alloy(Talladium™ alloy) and Co-Cr alloy(Wironium^(?) extra-hard alloy), which are widely used as partial denture frame work these days, depending on the cooling method, followed by casting. The frist group was bench cooled at room temperature(20℃), the second group was slowly cooled in the furnace from 700t to room temperature, and third, rapidly cooled in 20~22℃ water. The micro-structures of each specimen were compared by means of photomicrograph taken by metallurgical microscope. In addition to the physical characteristics of each specimen were obtained using the Rockwell C Scale. The results were obtained as follows: 1) The hardness value of Talladium™ alloy appeared higher than the Wironium^(?) alloy. 2) The hardness value of the slow cooling method(in the furnace) appeared higher the than the bench cooling method, water cooling method. 3) The grain particle in the slowly cooled alloy were much finer than the bench cooling method, water cooling method. 4) In the cooling method of Talladium™ alloy and Wironium^(?) alloy for partial denture the slow cooling method was considered more profitable than the bench cooling method, water cooling method.

필라델피아 염색체 양성 급성골수성백혈병에서 Imatinib Mesylate로 분자학적 관해를 유도한 후 성공적인 자가 말초혈액 조혈모세포이식이 시행되었던 1예

김여경,이제중,이병환,변정래,조덕,이일권,정익주,양동욱,김형준 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

저자들은 필라델피아 염색체 양성 급성골수성백혈병 환자에서 복합항암화학요법으로 혈액학적 완전관해를 유도하고, imatinib mesylate를 이용하여 분자학적 관해를 유도한 후, 성공적으로 자가 말초혈액조혈모세포이식을 시행하였던 1예를 경험하였기에 이를 보고하는 바이다. Philadelphia chromosome (Ph¹)-positive acute myeloid leukemia (AML) is very rare disease, comprising 3~4% of the AML, and is a clinically distinct entity with poor clinical outcome with conventional chemotherapy. We report a case of Ph¹-positive AML who underwent successful autologous peripheral blood stem cell transplantation after achieving a molecular remission with imatinib mesylate.

多樣한 黃芩藥鍼製劑의 安全性 및 效能에 關한 硏究

김호경,마진열,전원경,윤수영,강은정,주혜정,고병섭 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

In order to detect the safety and effect of various aqua-acupunctures from Scutellariae Radix, the modifications of boiling, filtration and dilution were employed for the manufacture of aqua-acupunctures. We injected 0.2cc of aqua-acupunctures into Joksamri(足三里) of rat, repeatedly. we compared subacute toxicity of them with saline group, distilled water(D.W.) group, acupuncture group and control group. The results were summarized as follows: 1. The groups were all healthy and alive, and there was no special abnormality in physical condition and autopsy. And there were not any toxic symptoms in repeating application of aqua-acupunctures to the rat, including changes of body weight, organ weight, haematological examination and serum biochemical test. 2. There was slight change of body weight in acupuncture group : We could see significance after 3 days (p<0.05) and after 7 days (p<0.001) in body weight loss. After 9 days, all tested groups were suppressed in body weight increment. 3. Result of organ weight: In Palking aqua-acupuncture(D-2 group), saline group and acupuncture group there were some statistical significance. Especially, acupuncture group revealed significant result in liver and spleen than aqua-acupunctures. From this result, we could suggest that the efficacy of acupuncture was preceded herbal medicine. 4. In serum biochemical test, we examined glucose(GLU), triglyceride(TG) and cholesterol(CHOL). In comparison with control group, the diluted 10 times of hwanggum aqua-acupuncture (× 10 group) was recognized significant decrease of glucose, but the diluted 100 times of Hwanggum aqua-acupuncture (× 100 group), D-2 group, saline group were confirmed significant increment. There was not any meaningful change of CHOL in all of tested group, excepting the acupuncture group was exhibited statistically significant decrease(p<0.05). In TG level all tested group except complex injection of standard compound (CPA group) and HG, there were significant value in statistically. The diluted solution was more significant decrease than Hwanggaum aqua-acupuncture(HG). The mutual relationship of components of aqua-acupuncture tended to decrease level of TG, regardless of its concentration. In acupuncture group, we gained some interesting result in meaningful decrease in TG. 5. Haematological examination showed significant increment of gramulocytes(GR) in all tested groups except Hwanggum aqua-acupuncture. And the diluted solutions of HG expressed very high increment of them(p<0.001). The GR and Mean Corpuscular Volume(MCV) of acupuncture group showed statistical significance.

週5일 근무제도가 文化觀光에 미치는 영향 : 光州·全南地域을 中心으로 Focusing on Gwang-Ju and Chunnam Area

金昞元,李章柱 한국문화관광학회 2001 문화관광연구 Vol.3 No.2

This studying shows a lot of influences on cultural tourism after we adopt the new system that we work only five day, in a week through Gwang-ju and Chunnam area. The government is trying to adopt a five day week system in order to improve the quality of our lives and to create for those who are unemployed. But we can't figure out clearly when our government adopt the system, because the government, employees and employers an agreement has not been reached. I except the new system will result in many advantages to our culture tourism. Therefore, first of all, I had formed some basic conceptions of that system. And I also analyzed some countries to figure out when they began to practice that system and the purpose of practice. I showed some plans of government for that system at the same time. Second, I examined some meanings and forms of cultural tourism, projects of the government that have proceeded to increase some areas already practicing the five day work week in order to make plans best suited for business. Third, we took a survey of people who reside in the Gwang-ju Chunnam area to known about they would use their extra free time after they adopt that system. Last of all I studied the new system to find out the social, economical effects on cultural tourism.

만성 심부전환자의 혈액에서 측정한 Tumor necrosis factor-α 및 Interleukin-6의 임상적 의의

김명구,김성구,박상호,한대희,강진환,변정득,심규혁,최병조,방덕원,온영근,현민수,권영주 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background and objectives: Many conditions are responsible for the pathophysiology and progressive mechanisms of congestive heart failure. More recently, it has also become evidence that another class of biologycally activated molecules generically reffered to as cytokine these are also over expressed in congestive heart failure. Therefore, the purpose of this study was to measure concentrations of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) in mild to severe symptoms of heart failure and compare their values with those found in normal control and analysed correlation relationship between cytokine level, clinical findings and hemodynamic indicies. Subjects and Methodology: Levels of TNF-α and IL-6 were measured on pulmonary artery during cardiac catheterization in heart failure patients(n=32) and normal subjects(n=8) as well as physical examination and echocardiogram. Cytokines assay were performed on plasma using commercially available ELISA(Enazyme-Linked Immunosorbent Assay) kits. Results: Although the levels of TNF-α and IL-6 tend to increase in congestive heart failure group, the cytokines level was not made significantly statistical difference between congestive heart failure group and controls. When analyzing the correlation between the levels of PCWP(pulmonary capillary wedge pressure) and cytokines(TNF-α, IL-6), respectively, there were statistically significant correlation coefficient 0.32,(p<0.05), 0.39(p<0.01). The cytokine IL-6 and pressure of pulmonary artery were significant correlation.(correlation coefficient 0.36, p<0.02) More significantly, there was correlated with TNF-α and IL-6.(correlation coefficient 0.57, p<0.001) Conclusions: There was tended toward high concentration of TNF-α & IL-6 in congestive heart failure and significant difference for PCWP between TNF-α & IL-6, thus may be correlated with development and progression in congestive heart failure.

시(視)표현의 통사·의미부 기술연구 : 예술학의 분석적 수준과 인지심리학적 기초 On the analytical level of Science of Art and It's Cognitive Foundations

김복영,박병주 홍익대학교 산업디자인 연구소 2003 미술디자인 논문집 Vol.- No.8

This study aims a t the observation of some analytical levels -especially those of syntactico-semantical- of the visual expressions in general. As far as the syntactical parts are concerned, there were two steps t o be approached. One is to select the paradigms according to the Saussure-Hambidgean suggestions, the other to derive syntagmas from what is called the 'chaos-system', one of nonlinear dynamics. In result, paradigms could be acquired 5 sorts with its so many varieties, as visual templates (VT), supplemented by their reciprocals(R) and complements(CLE) , and syntagmas five orbits from each constant c=0 to c=l0. In additional, in connection with semantic parts, the study introduced a Gremasian model of discourses and narratives for the interpretation of signifiers. By this, we could cover the conjunctive relationships(+) and the disjunctive(-) of the whole structure of visual expression. Finally, we certified that the above could be fully approved in terms of recent worldly cognitive scientic psychological discoveries.

심동적 영역의 평가에서 문항반응이론의 활용

김종택,고병구,김주학 師範大學 體育硏究所 1998 서울大學校 體育硏究所論集 Vol.19 No.2

The purpose of this study was to introduce item response theory(IRT) and to explore the possible applications to the measurement of psychomotor behavior. The primary assumption of IRT is an individual will respond to an item on a test can be described in terms of a probabilistic function that describes the probability of an examinee responding correctly to an item. By contrast with the classical test theory, the advantage of IRT is to be able to estimate examinee's true ability and the estimate does not depend on the other examinees or on the particular test administered. When the item response of an examinee is dichotomous, if the probability of an examinee responding correctly to an item would be dependent of the ability of the examinee and the difficulty of the item, Rasch Model or 1-parameter IRT model could be used for estimating the item parameters. But if the discriminant or guess factor would be considered, then 2-parameter of 3-parameter model could be used. However, when the item response is polytomous, polytomous response model such as Partial Credit Model, Rating Scale Model, Nominal Response Model can be used. Especially, Binomial Trial Model, Poisson Counts Model, and Inverse Binomial Model can be used for analyzing multiple-attempt, single-item skill tests of psychomotor domain. Through reviewing the literatures of IRT, item bank, computer adaptive testing, mastery test measurement of change, repeating skill test, and sequential test has been proposed as the possible applications for psychomotor domain. In conclusion, for more valid and reliable assessment in the pychomotor domain, IRT and the possible applications of IRT should be seriously considered in measurement research in physical education.