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Mouse(ICR)에 있어서 시험물질 열다한소탕(熱多寒少湯) 추출액의 급성독성 시험
마진열,황대선,이시우,김종열,신현규,Ma, Jin-Yeul,Huang, Dae-Sun,Lee, Si-Woo,Kim, Jong-Yeol,Shin, Hyeun-Kyoo 사상체질의학회 2009 사상체질의학회지 Vol.21 No.1
1. Objectives This study was designed to andyze the acute toxicity and safety of Yeoldahanso-tang 2. Methods We investigated the acute toxicity for water-extracted Yeoldahanso-tang. 25 male and 25 female mice were observed for 14 days after one day oral administration of Yeoldahanso-tang at the respective doses of 0(control group), 2560, 3200, 4000 and 5000 mg/kg. 3. Results We observed survival rates, general toxicity, change of body weight and autopsy. 4. Conclusions The data confirmed that Yeoldahanso-tang is free from the toxicity and safety problems in oral route respectively. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 mg/kg). In conclusion, LD50 of Yeoldahanso-tang was over 5000 mg/kg and it is very safe to mice.
양격산화탕(凉膈散火湯) 추출액이 ICR mouse에서의경구 투여 급성독성에 미치는 영향
마진열,황대선,서창섭,이시우,김종열,신현규,Ma, Jin-Yeul,Huang, Dae-Sun,Seo, Chang-Seob,Lee, Si-Woo,Kim, Jong-Yeol,Shin, Hyeun-Kyoo 사상체질의학회 2009 사상체질의학회지 Vol.21 No.3
1. Objectives: The aim of this study is data analysis for acute toxicity and safety of Yangkyuksanhwa-tang. 2. Methods: We investigated the acute toxicity for water-extracted Yangkyuksanhwa-tang. 25 male and 25 female mice were observed for 14 days after one day oral administration of Yangkyuksanhwa-tang at the respective doses of 0(control group), 2560, 3200, 4000 and 5000 mg/kg. 3. Results: We observed survival rates, general toxicity, change of body weight and autopsy. 4. Conclusions: The data confirmed that Yangkyuksanhwa-tang is free from the toxicity and safety problems in oral route respectively. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 mg/kg). In conclusion, LD50 of Yangkyuksanhwa-tang was over 5000 mg/kg and it is very safe to mice.
마진열,신현규,성현제,전원경,김인락,고병섭,정규용 한국독성학회 1997 Toxicological Research Vol.13 No.4
Scopoliae rhizoma is a perennial herb which has a similar effect with atropine on the cardiovascular system. It is also known to have a seditive and anticonvulsant activity on the central nerve system. In order to evaluate an acute toxicity of Scopoliae rhizoma, the present study was performed after administration the Scopoliae rhizoma prepared by both decoctional and frozen dried extract through three different routes (oral; 5,000 mg/kg, intraperitoneal; 2,000 mg/kg, subcutaneous; 5,000 mg/kg) to the female ICR mice. In the group treated intraperitoneally with a frozen dried extract, abnormal clinical signs such as decreased activity, crouch, potosis and abnormal walking were observed for 40 rain after administration. With regard to WBC, decreased number of lymphocyte and increased number of monocyte and granulocyte were also observed in the animals received intraperitoneally with Scopoliae rhizoma extract. Taken together, what toxicity of Scopoliae rhizoma was shown differently depending on its type for administration may be resulted in the differency of administered dose. The results provided here support a pharmacological and toxicological consideration for its clinical use in the regard of oriental medicine.
Chiness hamster lung cell(CHL)에서 십전대보탕 염색체 이상 시험
마진열,황대선,이남헌,하혜경,유영법,신현규,Ma, Jin-Yeul,Huang, Dae-Sun,Lee, Nam-Hun,Ha, Hye-Kyung,Yu, Young-Beob,Shin, Hyun-Kyoo 대한한의학방제학회 2008 大韓韓醫學方劑學會誌 Vol.16 No.2
Objectives : This study was to assessment the toxicity of Sipjeondaebo-tang(Shiquan dabu-decoction) by Chromosomalanomaly test. Methods : Sipjeondaebo-tang(Shiquan dabu-decoction) water-extract in vivo Chromosomalanomaly test was performed using chiness hamster lung cell line. Results : Sipjeondaebo-tang water extract was negative in Chromosomalanomaly test at the doses of 0, 625, 1250 and $2500{\mu}g/m\ell$ at 6h and 24h.(S9- fraction). Chromosomalanomaly test(S+fraction) was also negative at the doses of 0, 1250, 2500 and $5000{\mu}g/m\ell$. Conclusions : It was concluded that Sipjeondaebo-tang extract did not induce Chromosomalanomaly in the chiness hamster lung cell.
Mouse에 있어서 시험물질 청심연자탕(淸心蓮子湯) 추출액의 급성독성 시험
마진열,황대선,서창섭,이시우,김종열,신현규,Ma, Jin-Yeul,Huang, Dae-Sun,Seo, Chang-Seob,Lee, Si-Woo,Kim, Jong-Yeol,Shin, Hyeun-Kyoo 사상체질의학회 2010 사상체질의학회지 Vol.22 No.1
1. Objectives: The aim of this study is data analysis for acute toxicity and safety of Cheongsimyeonja-tang. 2. Methods: We investigated the acute toxicity for water-extracted Cheongsimyeonja-tang. Fifty five male and female mice were observed for 14 days after one day oral administration of Cheongsimyeonja-tang at the respective doses of 0 (control group), 2560, 3200, 4000 and 5000 mg/kg. 3. Results: We observed survival rates, general toxicity, change of body weight and autopsy. In animals administered with Cheongsimyeonja-tang, there were nither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign and autopsy. 4. Conclusion: The data confirmed that Cheongsimyeonja-tang is free from the toxicity and safety problems in treated groups. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 mg/kg). Lethal Dose 50 (LD50) value for mice was more than 5000 mg/kg per oral for both male and females. It suggest that Cheongsimyeonja-tang in mice is considered to be safe.
ICR 마우스를 이용하여 사물탕(四物湯)의 급성 독성에 관한 연구
마진열,유영법,하혜경,황대선,마충제,신현규,Ma, Jin-Yeul,Yu, Young-Beob,Ha, Hye-Kyung,Huang, Dae-Sun,Ma, Choong-Je,Shin, Hyun-Kyoo 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2
Objectives: Samul-tang(Siwu-tang) has been traditionally prescribed a medicine as a restorative. Methods: In this study, we investigated the acute toxicity about water-extracted Samul-tang(Siwu-tang). Twenty-five mice completed 14 days of oral Samul-tang(Siwu-tang) at the respective doses of 0(control group), 2560, 3200, 4000 and 5000mg/kg. Results: We observed survival rates, general toxicity, change of body weight, and autopsy. Conclusions: Compared with the control group, we could not find any toxic alteration in all treated groups (2560, 3200, 4000 and 5000mg/kg). $LD_{50}$ of Samul-tang(Siwu-tang) was over 5000mg/kg and it is very safe to ICR mice.
태음조위탕(太陰調胃湯) 추출액이 ICR mouse에서의 경구 투여 급성독성에 미치는 영향
마진열,황대선,서창섭,이시우,김종열,신현규,Ma, Jin-Yeul,Huang, Dae-Sun,Seo, Chang-Seob,Lee, Si-Woo,Kim, Jong-Yeol,Shin, Hyeun-Kyoo 사상체질의학회 2010 사상체질의학회지 Vol.22 No.2
1. Objectives: The aim of this study is to investigate the acute toxicity and safety of Taeeumjowi-tang. 2. Methods: We investigated the acute toxicity for water-extracted Taeeumjowi-tang. 25 male and 25 female mice were observed for 14 days after one day oral administration of Taeeumjowi-tang at the respective doses of 0(control group), 2560, 3200, 4000 and 5000 mg/kg. 3. Results: We observed survival rates, general toxicity, change of body weight and autopsy. 4. Conclusions: The data confirmed that Taeeumjowi-tang is free from the toxicity and safety problems in oral route respectively. Compared with the control group, we could not find any toxic alteration in all treated groups(2560, 3200, 4000 and 5000 mg/kg). In conclusion, LD50 of Taeeumjowi-tang was over 5000 mg/kg and it is very safe to mice.
Vibrio parahemolyticus에 대한 한방처방 [方藥合編] 및 그 단미제의 항균활성에 관한 연구
마진열,김진숙,신순식,정규용,박갑주 한국한의학연구원 1999 한국한의학연구원논문집 Vol.5 No.1
Vibrio are become prevailing if superficial temperature of ocean is raised and their activities of area are expanded and most of ocean creatures (fishes, oysters etc) are polluted with vibrio. The one who has taken these polluted fishes and aysters uncooked caused foodpoisoning and diarrhea from Vibrio. Frequencise of these diseases breakout is disposed in westsea shore of Korea. According to ancient and traditional Korean medical book -『Bangyak Happyeon』(Collection of Local Medicines, 1884) - and their single prescibes, we carried out experiment check the activities of natural medicinal effects on Vibrio parahemolyticus. The prescriptions of trial materials are processed from extraction boiling water and 80% methanol and followed freeze dried and adsorbed to every discs in dosage of 10mg. Gentamycin of 10mg were used for control. The result of compound prescription displayed special diseases in antimicrobial activities of boiling water and MeOH extraction compared with control. In compound prescription, extraction MeOH of Sashinhwan(clear zone : 17mm) presented extraordinaire antimicrobial activity. In single prescription, extraction of boiling water (clear zone : 16mm) and MeOH(clear zone : 18mm) of Fructus Chebulae presented extraordinaire antimicrobial activity. The MBC of Fructus Chebulae extracts was expressed in boiling water (1.28mg/ml) and MeOH(0.64mg/ml).