A genetic variation in microRNA target site of <i>KRT81</i> gene is associated with survival in early-stage non-small-cell lung cancer

Lee, S. Y.,Choi, J. E.,Jeon, H. S.,Hong, M. J.,Choi, Y. Y.,Kang, H. G.,Yoo, S. S.,Lee, E. B.,Jeong, J. Y.,Lee, W. K.,Lee, J.,Cha, S. I.,Kim, C. H.,Kim, Y. T.,Jheon, S.,Son, J. W.,Park, J. Y. Oxford University Press 2015 ANNALS OF ONCOLOGY Vol.26 No.6

<P>In this study, <I>KRT81</I> rs3660G>C was associated with survival of patients with NSCLC after surgical resection. Mechanistic study suggested that the G-to-C change caused reduced binding efficiency of miRNA, leading to decreased translational repression, thereby increased <I>KRT81</I> expression. The <I>KRT81</I> rs3660G>C may be a useful prognostic biomarker in early-stage NSCLC patients.</P><P><B>Background</B></P><P>MicroRNAs (miRNAs) have a key role in carcinogenesis through negative regulation of their target genes. Therefore, genetic variations in miRNAs or their target sites may affect miRNA–mRNA interactions, thereby result in altered expression of target genes. This study was conducted to investigate the associations between single-nucleotide polymorphisms (SNP) located in the miRNA target sites (poly-miRTSs) and survival of patients with early-stage non-small-cell lung cancer (NSCLC).</P><P><B>Methods</B></P><P>Using public SNP database and miRNA target sites prediction program, 354 poly-miRTSs were selected for genotyping. Among these, 154 SNPs applicable to Sequenom's MassARRAY platform were investigated in 357 patients. A replication study was carried out on an independent patient population (<I>n</I> = 479). <I>Renilla</I> luciferase assay and reverse transcription-polymerase chain reaction were conducted to examine functional relevance of potentially functional poly-miRTSs.</P><P><B>Results</B></P><P>Of the 154 SNPs analyzed in a discovery set, 14 SNPs were significantly associated with survival outcomes. Among these, <I>KRT81</I> rs3660G>C was found to be associated with survival outcomes in the validation cohort. In the combined analysis, patients with the rs3660 GC + CC genotype had a significantly better overall survival compared with those with GG genotype [adjusted hazard ratio (aHR) for OS, 0.65; 95% confidence interval (CI) 0.50–0.85; <I>P</I> = 0.001]. An increased expression of the reporter gene for the C allele of rs3660 compared with the G allele was observed by luciferase assay. Consistently, the C allele was associated with higher relative expression level of <I>KRT81</I> in tumor tissues.</P><P><B>Conclusion</B></P><P>The rs3660G>C affects KRT81 expression and thus influences survival in early-stage NSCLC. The analysis of the rs3660G>C polymorphism may be useful to identify patients at high risk of a poor disease outcome.</P>

Influences of Enzyme Complex Supplementation on Growth, Ileal and Apparent Fecal Digestibility and Morphology of Small Intestine in Pigs

Kim, B.G.,Tian, J.Z.,Lim, J.S.,Kil, D.Y.,Jeon, H.Y.,Chung, Y.K.,Kim, Y.Y. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.12

A total of 140 weaning pigs were used to determine the effects of digestive enzyme supplementation to corn-soybean meal diets on growth performance, physiological changes of small intestine, microorganisms and pH in the gastrointestinal tract. Two kinds of enzyme complex (A, B) were used in this experiment. Pigs were allotted in a completely random design (CRD) to five replicates with four pigs per pen. Diets and water were provided for ad libitum consumption. Treatments included 1) Control: without enzyme supplementation, 2) Enzyme A 0.05%, 3) Enzyme A 0.10%, 4) Enzyme A 0.15%, 5) Enzyme B 0.05%, 6) Enzyme B 0.10%, 7) Enzyme B 0.15% in the diets. A total of 24 crossbred barrows 25.78${\pm}$0.55 kg BW fitted with simple ileal T-cannulas were used to evaluate the effect the enzyme addition on the nutrient digstibility. Pigs were allotted 4 treatments (No enzyme, enzyme A 0.05%, enzyme A 0.1%, enzyme A 0.15%), 6 replicates according to a completely random design (CRD). Another digestibility trial was followed for enzyme complex B. Twenty pigs, average 31.92${\pm}$0.37 kg BW, fitted with simple ileal T-cannulas for digestibility trial. Neither enzyme A nor enzyme B affected on fecal or ileal digestibility of dry matter, gross energy, crude protein, crude fat and crude ash (p>0.05). The apparent fecal digestibilities of all the nutrients were higher in total feces collection method than in indirect method. At the end of feeding trial, 21 pigs were slaughtered for examining the morphological changes of small intestine and the concentration of microorganisms in the ileum and the colon. Growth performance, intestinal morphology and pH of ileum and colon were not affected by the either enzyme complex supplementation (p>0.05). These results suggested that enzyme complex A and enzyme complex B were of no benefit to early-weaned pigs when corn-soybean meal based diet was provided.

국내 HIV/AIDS 발견이후 HIV 유행주의 분자역학적 변이 분석

이주실,김성순,남정구,전수경,변승옥,최병선,박미선,김옥진,서순덕 국립보건연구원 2000 국립보건원보 Vol.37 No.-

G-code control 을 이용한 웹 기반 금형 가공 공정 모니터링 & 컨트롤 시스템 개발

김건희(G. H. Kim),최진화(J. H. Choi),전병철(B. C. Jeon),조명우(M. W. Cho) 한국정밀공학회 2004 한국정밀공학회 학술발표대회 논문집 Vol.2004 No.10월

The target of this paper is the development of Web-based monitoring & control system which is for effective and economic management of mold manufacturing process. This system has three module; G-code Control, Monitoring Module and Result analysis module. Also, as the environment of development is based on internet, this system which is possible to the remote site management of manufacturing process works on Web. To be possible to control the manufacturing monitoring by client, each module is made ActiveX control and is based on socket communication. This system makes the foundation which is possible to manage the mold manufacturing process efficiently from remote site by matching real-time monitoring with manufacturing process in factory using G-code control and displaying the result of manufacturing using Ch-CGI.

UPFC가 계통에 미치는 영향을 선로정수 G,B의 함수로 표현한 새로운 UPFC 정태 해석모델 개발

전동훈(D.H. Jeon),김태균(T.G. Kim),추진부(J.B. Chu),김건중(K.J. Kim) 대한전기학회 1999 대한전기학회 학술대회 논문집 Vol.1999 No.7

검정소 검정돈의 품종 및 환경요인의 효과 추정

박종원,김병우,김현철,이길왕,최진성,강왕근,홍성광,하정기,전진태,이정규 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

This study was carried out to estimate the effects of breed and environment such as sex, test station, test year, test season, parity, initial and final weight on average daily gain, age at 90㎏, backfat thickness, feed efficiency, lean percent and selection index on the basis of the performance data collected from 25,790 pigs of Duroc, Yoskshire and Landrace breeds which were performance-tested at the Korea Swine Testing Station from 1991 to 2002. The results obtained in the study are summarized as follows; 1. The means of the major economic traits were estimated as 959.95 ± 0.699g for average daily gain, 138.36 ± 0.072days for age at 90㎏, 1.41 ± 0.001㎝ for backfat thickness, 2.33 ± 0.001 for feed efficiency, 56.71 ± 0.018% for lean percent and 221.65 ± 0.113 for selection index. 2. The effect of breed was statistically significant for all studied traits. Briefly, Duroc showed the best per-formance for the average daily gain and age at 90㎏. Landrace had the best performances for the backfat thickness and lean meat percent. In feed efficiency and selection index, Yorkshire had a better score than other breeds. 3. The least-squares means of female and male for the traits studied were 923.05 ± 1.289g and 974.53 ± 0.856g for average daily gain, 139.74 ± 0.145days and 137.21 ± 0.097days for age at 90㎏, 1.49 ± 0.002㎝ and 1.39 ± 0.002㎝ for backfat thickness, 2.43 ± 0.002 and 2.28 ± 0.002 for feed efficiency, 56.43 ± 0.034% and 56.81 ± 0.023% for lean percent and 211.37 ± 0.194 and 224.61 ± 0.129 for selection index. Therefore, males were superior to females for all traits examined. 4. The effect of test station was statistically significant for all traits except for selection index. Performances for age at 90㎏, backfat thickness, feed efficiency and lean meat percent collected from Test station 2 were higher than those from Test station 1. However. Test station 1 showed better average daily gain. 5. The initial weight and final weight included as a covariate in this study had a significant influence on average daily gain, age at 90㎏, backfat thickness, feed efficiency and selection index. From the absolute values of the estimated regression coefficients, it was inferred that the final weight had greater effect for the investigated traits than the initial weight.

Anchoring sulfonic acid on silica surface through Si?C bond for immobilization of catalyst for polyketone synthesis

Eo, S.C.,Lee, S.,Park, G.H.,Jeon, J.Y.,Lee, B.Y. Elsevier Science Publishers 2016 CATALYSIS TODAY - Vol.265 No.-

<P>Sulfonic acid groups were anchored on a silica surface through robust Si-C bonds. The successive treatment of dehydroxylated silica with benzylmagnesium chloride and H2SO4 resulted in the surface tethering of -CH2C6H4SO3H groups at a high coverage rate (0.50-CH2C6H4SO3H/nm(2)). The pore structure of the silica remained unchanged during this surface-modification process. Next, the -CH2C6H4SO3H groups on the surface were successfully used for preparing a supported catalyst for CO/ethylene copolymerization; the Si-CH2C6H4SO3H groups on the surface were reacted with [1,3-bis(di(2-methoxyphenyl)phosphino)propane]Pd-( OAc)(2) to generate dicationic palladium species, which were anchored on the silica surface through ionic interactions with the sulfonate anions generated on the surface. The supported catalyst prepared in this way exhibited a high activity (up to 43 kg/g-Pd or 0.61 kg/g-cat) with respect to CO/ethylene copolymerization. The morphology of the obtained polymer particles replicated that of the silica particles. Thus, a polymer powder that exhibited a high bulk density (0.30 g/mL) could be obtained while causing minimal reactor fouling. (C) 2015 Elsevier B.V. All rights reserved.</P>

한우 암소의 개체별 성장곡선 모수 추정

이창우,최재관,전기준,나기준,이채영,황정미,김병완,김종복 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

Weight records of Hanwoo cows from birth to 36 months of age collected in Daekwanryeong branch, National Livestock Research Institute(LNRI) were fitted to Gompertz, von Bertalanffy and Logistic functions. For the growth curve parameters fitted on individual records using Gompertz model, the mean estimates of mature weight(A), growth ratio(b) and growth rate(k) were 383.42±97.29㎏, 2.374±0.340 and 0.0037±0.0012, respectively, and mean estimates of body weight, age and daily gain rate at inflection were 141.05±35.79㎏, 255.63±109.09 day and 0.500±0.123㎏, respectively. For von Bertalanffy model, the mean estimates of A, b and k were 410.47±117.98㎏, 0.575±0.057 and 0.003±0.001, and mean estimates of body weight, age and daily gain at inflection were 121.62±34.94㎏, 211.02±105.53 and 0.504±0.124㎏. For Logistic model, the mean estimates of A, b and k were 347.64±97.29㎏, 6.73±0.34 and 0.006±0.0018, and mean estimates of body weight, age and daily gain at inflection were 173.82±37.25㎏, 324.47±126.85 and 0.508±0.131㎏. Coefficients of variation for the A, b and k parameter estimates were 25.3%, 14.3% and 32.4%, respectively, for Gompertz model, 28.7%, 9.9% and 33.3% for von Bertalanffy model, and 27.9%, 5.0% and 30.0% for Logistic model.

한우 암소의 성장곡선 모수에 영향을 미치는 요인

이창우,최재관,전기준,나기준,이채영,황정미,김종복 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

Some growth curve models were used to fit individual growth of 1,083 Hanwoo cows born from 1970 to 2001 in Daekwanryeong branch, National Livestock Research Institute(NLRI). The effects of year-season of birth and age of dam were analyzed. In analysis of variance for growth curve parameters, the effects of birth year-season were significant for mature weight(A), growth ratio(b) and maturing rate(k)(P<.01). The effects of age of dam were significant for growth ratio(b) but not significant for mature weight(A) and maturing rate(k). The linear term of the covariate of age at the final weights was significant for the A(P<.01) and k(P<.01) of Gompertz model, von Bertalanffy model and Logistic model. For the growth curve parameters fitted on individual data using Gompertz model, von Bertalanffy model and LOgistic model, rusulting the linear contrasts(fall-spring), Least square means of A in three nonlinear models were higher cows born all fall and A of Logistic model was significant(P<.05) between the seasons. According to the results of the least square menas of growth curve parameters by age of dam, least square means of mature weight(A) in Gompertz model was largest in 6 year and smallest estimating for 3 and 8 years of age of dam. The growth ratio(b) was largest in 2 year of age of da and smallest estimating in 8 year. The A and k were not different by age of dam(p>.05), On the other hand, the b was different by age of dam.(p<.01). The estimate of A in von Bertalanffy model was largest in 6 year and smallest in 8 and 9 years of age of dam. The b was largest in 2 year and tend to decline as age of dam increased. The A and k were not different by age of dam(p>.05), On the other hand, the b was highly significant by age of dam(p<.01).

한국 재래 닭 품종 특성 및 초기성장 개량을 위한 분자표지 개발

오재돈,박미현,공홍식,이학교,전광주,연성흠,상병돈,최철환,조병욱,Oh J. D.,Park M. H.,Kong H. S.,Lee H. K.,Jeon G. J.,Yeon S. H.,Sang B. D.,Choi C. H.,Cho B. W. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.1

This study was conducted to estimate the effects of genotype for chicken major histocompatibility complex (MHC) B-LB genes on economic traits. To detect polymorphism, 400 bp fragments of MHC B-LB genes were obtained and sequenced. After digestions using restriction enzyme Hea III, two restriction enzyme sites were observed. There were two mutations at position 427 and 651 those were decided as Type I and Type II, respectively. Using RFLP analyses, type I were genotyped to TT, TC and CC, and type II to MM, Mm and mm. The relatively higher TC genotype frequencies (0.8) of Type I and Mm genotype frequencies (0.88) of Type II were observed in Korean native chickens. The effects of the genotype on 150 days body weight trait were investigated by the associations of CC and Mm genotypes (P<0.05) in Korean native chickens. This result suggests that a significant association exists between the SNP and 150 days body weight. 본 연구는 한국 재래 닭의 유전적 특성을 분자표지를 이용하여 그 차이를 규명하고 초기성장에 미치는 영향을 분석하여 이를 이용한 재래닭의 개량을 목적으로 실시하였다. MHC class II B-LB 유전자 내의 염기변이체가 경제형질에 미치는 영향에 대하여 연구하였다. MHC class II B-LB유전자 내 400 bp 크기의 유전자를 증폭하여 염기서열 분석과 제한효소 처리를 이용한 다형성 분석을 실시하였다. 연구결과 두 개의 제한효소 절단지역이 발견되었으며 427 지역을 Type I 으로 651 지역은 Type II로 정하여 RFLP 분석을 실시하였다. Type I지역의 유전자형은 TT, TC, CC로 나타났으며, TypeII 지역의 유전자형은 MM, Mm, mm으로 나타났다. TC와 Mm 유전자형이 다른 유전자형과 비교하였을 때 한국재래 닭에서 높은 출현빈도를 보였다(0.8, 0.88). 유전자형이 한국 재래 닭의 150일령 체중에 미치는 영향을 분석한 결과 CC와 Mm 유전자형에서 통계적 유의성이 도출되었다 (P<0.05). 따라서 본 연구의 결과를 이용하여 한국 재래 닭의 유전적 특성을 규명할 수 있으며 초기 성장이 높은 성적을 나타내는 CC, Mm 유전자형을 개량에 이용하게 된다면 큰 효과를 얻을 수 있을 것으로 사료되어진다. 본 연구의 결과는 차후 한국 재래 닭의 과학적이고 지속적인 유전자원의 보존과 육종 전략에 있어 매우 유용하게 활용될 것으로 기대된다.