A Study on the Functionality of Halophytes from Coast area of Jeollanam-do

Tae-Man Ha,Yang-Joon An,Mi-Yeong Shin,Sook Park,Hak-Jae Park,Hyo-Jeong Kang,Su-Jeong Choi,Jae-Yeong Yu,Ji-Yun Jeon,Gyu-Yeon Oh 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

This study was undertaken to improve the perceived value of halophytes and contribute to the activity of the local economy by analyzing the antioxidant activity and mineral content of Suaeda japonica, Tetragonia tetragonoides, and Spergularia marina that grow wild on the mud flats and coastal areas. The total phenolic compound content found in each order is as follows: Suaeda japonica (3.674∼5.989 mg TAE/g dry wt.), Tetragonia tetragonoides (2.515∼4.471 mg TAE/g dry wt.), and Spergularia marina (2.450∼4.067 mg TAE/g dry wt.); DPPH radical scavenging and ABTS cation radical scavenging activities were also recorded in the same order. In the case of minerals, Iron(Fe) was relatively high in Suaeda japonica (960.1∼1,346.7 mg/kg), Manganese(Mn) in Tetragonia tetragonoides (197.1∼574.8 mg/kg), and Zinc(Zn) was relatively high in Spergularia marina (63.19∼216.56 mg/kg). It was confirmed that the mineral content of Suaeda japonica, Tetragonia tetragonoides, and Spergularia marina was significantly higher than that of Chinese cabbage, lettuce, leek, and spinach.

Long-term outcomes of single-port laparoscopic myomectomy using a modified suture technique

( Mina Kang ),( Jihye Kim ),( Tae-joong Kim ),( Jeong-won Lee ),( Byoung-gie Kim ),( Duk-soo Bae ),( Chel Hun Choi ) 대한산부인과학회 2020 Obstetrics & Gynecology Science Vol.63 No.2

Objective To evaluate the long-term outcomes, including the pregnancy outcome and recurrence rate after single-port laparoscopic myomectomy (LM) using a modified suture technique with a Hem-o-lok clip (Choi’s LM) and conventional 4-port LM. Methods A retrospective study of patients who underwent Choi’s LM (n=55) and 4-port LM (n=102) in a single institutional hospital was conducted. Patients with <3 symptomatic myomas sized <10 cm each and operated on by a single surgeon were included. Recurrence was confirmed when a myoma measuring ≥3 cm was detected. Results The patients in both groups had similar demographic characteristics. Single (76.4% vs. 62.7%) and intramural (52.7% vs. 56.9%) tumors were commonly detected in both groups in the mean diameter (6.8±1.5 cm vs. 7.0±1.6 cm; P=0.40). In Choi’s LM, 16 patients (29.1%) needed an additional port; those who were nulliparous and/or had a large leiomyoma more frequently required an additional port (P=0.023 and 0.04, respectively). During a median follow-up period of 69 months, 17 patients (7.1% vs. 14.6%) had recurrence. The size of dominant myomas at recurrence was significantly smaller in patients who underwent Choi’s LM (3.4±0.7 cm vs. 5.7±2.4 cm; P=0.004). All 13 patients in both groups who successfully conceived had a full-term delivery. No major complications occurred during pregnancy. Conclusion Although an additional port was frequently used, the long-term outcomes of patients who experienced recurrence and pregnancy after Choi’s LM were acceptable. Considering its usability, Choi’s LM is feasible for the treatment of uterine leiomyoma.

Effect of 2-Deoxy-D-Glucose on Metabolic Status, Proliferative Capacity and Growth Rate of FSall Tumor

Hyesook Chang(장혜숙) , Eun Kyung Choi최은경) , Jeong Gill Cho(조정길) , Tae-Hwan Lim(임태환) , Tae-Keun Lee(이대근) ,Yun Yi(이윤) ,Young Joo Cho(조영주) , Gon Sup Kim(김곤섭) 대한방사선종양학회 1991 Radiation Oncology Journal Vol.9 No.1

2-DDG가 쥐의 섬유육종(FSall)에 미치는 영향에 대한 연구로 에너지 신진대사는 체내에서의 ³¹P - 자기공명 분광기를 이용하여 관찰하였고 세포 증식 능력은 유세포 분석기를 사용하여 연구하였다. 성장속도는 개의 세포를 C₃Hj/Sed 쥐의 발등에 이식한 후 3차원적으로 측정하여 관찰하였다. 2-DDG를 투여한 경우에는 이식후 12일에 복강내로 주사하였다. 이식후 12일의 종양의 평균 크기는 250㎣이었다. FSall 종양의 성장속도는 semilog graph의 기울기와 종양의 doubling time으로 측정하였다. 2-DDG를 투여한 후 성장속도가 감속되었다. 5~12일 사이의 성장속도의 기울기가 0.828, 종양의 Idubling time이 0.84일이고 대조군에서는 13~28일 사이의 기울기가 0.218, doubling time이 3.2일인 반면 2-DDG 투여군에서는 성장속도의 기울기가 0.135이고 doubling time이 5.1일이었다. ³¹P-자기공명 분광기를 이용하여 2-DDG의 영향을 분석해 본 결과 2-DDG 투여후 종양증식 속도의 감속과 더불어 phosphomonester (PME)와 inorganic phosphate (Pi)의 증가속도가 감소하였다. 이것은 2-DDG 투여후 세포의 괴사가 감소하였다는 의미가 있다. 유세포 분석기를 이용하여 종양의 증식 능력을 분석한 결과는 2-DDG 투여후 S-phase와 G₂+M phase의 DNA 분포가 크게 증가하였다. 이것은 2-DDG 투여후 세포가 좀더 방사선에 민감한 cycle로 진행함을 의미하는 것으로 해석할 수 있다. 이에 저자들은 2-DDG가 FSall 종양세포에 미치는 흥미있는 결과를 토대로 방사선 치료에 미치는 영향과 실제 이용 가능성에 대하여 더 연구하고자 한다. The effect of 2-deoxy-d-glucose (2-DDG) on C₃H mouse fibrosarcoma(FSall) was studied, Metabolic status, especially for energy metabolism, was studied using in vivo P-MRS, prolifer-ative capacity was observed on flow cytometry(FC) and growth rate was measured after trans-plantation of viable tumor cells in the dorsum of foot of C₃Hf/Sed mice. One gram of 2-DDG per kg of body weight was injected intraperitoneally on 12th day of implantation. Average tumor size on 12th day of implantion was 250㎣. Growth rate of FSall tumor was measured by tumor doubling time and slope on semilog plot. After 2-DDG injection, growth rate slowed down. Tumor doubling time between tumor age 5-12 days was 3.2 days with slope 0.218 in control group. After 2-DDG injection, tumor doubling time was elongated to 5.1 days with slope 0.136. The effect of 2-DDG studied in vivo P-MRS suggested that the increase of phosphomonoes-ter (PME) and inorganic phosphate (Pi) by increasing size of tumor, slowed down after 2-DDG injection. Flow cytometry showed significantly increased S-phase and G₂ + M phase fraction suggesting increased proliferative capacity of tumor cells in the presence of 2-DDG. Authors observed an interesting effect of 2-DDG on FSall tumor and attempt to utilize as an adjunct for radiotherapy.

청심연자탕(淸心蓮子湯)으로 호전된 뇌내출혈에 고혈압이 동반된 태음인 환자 치험 1례

오정민,엄태민,최고은,허종원,유호룡,설인찬,김윤식,Oh, Jeong-Min,Eom, Tae-Min,Choi, Ko-Eun,Heo, Jong-Won,Yoo, Ho-Ryong,Seol, In-Chan,Kim, Yoon-Sik 대한중풍순환신경학회 2014 대한중풍.순환신경학회지 Vol.15 No.1

■ Objectives The purpose of this case study is to report that a hypertension with intracerebral hemorrhage of tae-eumin was treated with 'Cheongsim Yeunja-tang' and then the symptoms were improved and blood pressure was decreased. ■ Methods Although the patient who had hypertension with intracerebral hemorrhage took antihypertension drugs, intermittent increase of blood pressure was shown. We diagnosed him as Tae-eumin and treated with Cheongsim Yeunja-tang. We daily checked blood pressure and evaluated the symptoms. ■ Results After the treatment with Cheongsim Yeunja-tang, blood pressure was decreased and symptoms were improved. ■ Conclusion The result shows Cheongsim Yeunja-tang has antihypertensive effect and improve hypertension symptoms of Tae-eumin patient with intracerebral hemorrhage.

21가지 장미꽃 품종의 페놀성 화합물 함량 및 항산화 활성

조연재(Yeon Jae Jo),서정현(Jeong Hyun Seo),홍채영(Chae Young Hong),김성태(Seung Tae Kim),최은경(Ehn-Kyoung Choi),김윤배(Yun-Bae Kim),이준수(Junsoo Lee),정헌상(Heon Sang Jeong) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.4

본 연구는 21품종의 장미꽃에 대한 식품 및 화장품 원료로 사용 가능성을 살펴보기 위해 페놀성 화합물과 항산화 활성을 평가하였다. 총 폴리페놀, 총 플라보노이드 및 타닌 함량은 러버샤이 품종에서 각각 379.70 mg GAE/extract g, 50.10 mg CE/extract g 및 339.44 mg TAE/extract g으로 높게 나타났으며, 칠백리 품종에서 각각 107.25 mg GAE/extract g, 9.46 mg CE/extract g 및 36.31 mg TAE/extract g으로 낮았다. 페놀산 함량은 총 폴리페놀 함량과 유사한 결과를 보였으며, 러버샤이 품종에서 27.40 mg/extract g으로 높았다. 안토시아닌은 대부분 cyanidin-3,5-diglucoside로 확인되었으며 총 안토시아닌 함량은 유니나 품종에서 8.80 mg/extract g으로 가장 높았다. ABTS 및 DPPH 라디칼 소거 활성은 각각 428.10 및 419.59 mg AAE/extract g로 러버샤이 품종에서 가장 높았으며, 항산화 활성과 페놀 화합물의 함량과 높은 양의 상관관계를 보였다. 이러한 결과는 러버샤이와 같은 항산화 활성이 뛰어난 품종들이 관상 목적뿐만 아니라 높은 기능성을 가진 식품 및 화장품의 원료로 사용 가능성이 있다고 판단된다. This study evaluated the functional components and antioxidant activities of 21 different rose flower extracts, to confirm their value as a food and cosmetics ingredient. The highest values of total polyphenol, flavonoid and tannin contents (379.70±1.64 mg GAE/extract g, 50.10±0.76 mg CE/extract g, and 339.44±1.20 mg TAE/extract g, respectively) were obtained in Lover Shy. The proanthocyanidin content ranged from 61.88 mg CE/extract g in the Unina cultivar to 5.34±0.13 mg CE/extract g in the Aileen cultivar. The phenolic acid content obtained was highest in the Lover Shy cultivar (27.40±0.26 mg/extract g), whereas highest levels of anthocyanin (8.83±0.03 mg/extract g) were obtained in the Unina cultivar. High ABTS and DPPH radical scavenging activities were determined in the Lover Shy cultivar (428.10±0.29 and 419.59±3.12 mg AAE/extract g, respectively). Taken together, these results indicate that the antioxidant activity and functional components of Lover Shy cultivar are superior, and this extract can potentially be applied as a food and cosmetic ingredient.

형질전환 제초제저항성 벼 밀양 204호의 농업적 특성

정응기,이기환,원용재,박향미,전남수,최준호,구연충,한창덕,은무영,김태산,남민희 Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.

This study was conducted to investigated the major characteristics of genetically modified rice of "Milyang 204" originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Junambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of "Milyang 204" showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic "Milyang 204" showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the nontransgenic varieties rice.

Tsukamurella sp. 26A에 의한 생물계면활성제의 생산

최경숙,김순한,정영기,장경립,이태호 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

The strain producing biosurfactant was isolated from soil. The isolated strain was identified as the genus Tsukamurella through its morphological, cultural, physiological, menaquinone type, fatty acid composition characteristics. The highest biosurfactant production by Tsukamurella sp. 26A was observed after 4 days cultivation in the culture medium containing n-hexadecane 7%, NaNO₃0.2%, KH₂PO₄0.001%, K₂HPO₄0.02%, MgSO₄·7H₂O 0.02%, CaCl₂·2H₂O 0.02%, yeast extract 0.02%(pH 6.8-7.0, 30℃). The surface and interfacial tension of an aqueous solution reached 30 mN/m and 1.5 mN/m, respectively. The biosurfactant stabilized oil-in-water emulsion with a variety of hydrocarbones, edible oils and petroleum oils.

한국 일부지역의 이주근로자의 근로조건과 산업재해실태 조사

최재욱,김정아,김해준,박종태 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

This study was conducted to evaluate and to recognize the status and the problems of working conditions, and industrial accidents for migrant workers in a group of 342 who lives in Ansan area, Kyunggi province. By the questionnaire written by 5 languages(English, Bangladesh, Indonesian, Chinese and Korean), the informations of socio-demographic factors, occupational conditions of engaged company and general & occupational health status were obtained from June, 1st to July 30th, 1998. We used the ANOVA to examine differences of working hours, days and average monthly wages between overseas industrial trainee and illegal migrant workers, and by using logistic regression, odds ratio of general risk factors for industrial accidents in migrant workers were calculated. The results obtained were as follows: 1. The major distribution of nationals were from Philippine, Indonesia, Bangladesh and China(92.1%), and the most of subjects were man(78.9%) and over high school graduate(81.9%). 2. Most migrant workers worked at small scale companies under 50 employees, and engaged in dye·textile·clothing·leather industry. The average working hours were 59.9 hours per week in overseas industrial trainee, 64.9 hours in illegal migrant worker respectively. 3. Trainees have transferred to another company for 1.89 times, and workers who have changed qualification from trainee to illegal status were 3.09 time, and illegal workers moved 2.36 times. The major portion of reason to move to another company were low salary and overdue salary. 4. Trainees, workers who have changed position from trainee to illegal status, and illegal workers knows 62.27%, 42.31%, and 20.45% in each about legal documentation for foreign industrial trainees in Korea "Regulations for protecting and managing of foreign industrial trainees in Korea " published on March 1995. Additionally, trainees and illegal workers have experienced medical examination by 52.3% and 18.2% respectively. 5. Industrial accident condition from Jun. 1st, 1997 to Dec. 31th, 1997 were as follows : in case of frequent rate, trainees and illegal workers were 30.04, 23.78 each, and 8.6%, 7.4% for industrial accident rate respectively. 6. The most common causes and types of industrial accident were inappropriate motions of body and abrasion, and body sites most vulnerable to injury were the hands and fingers. The results suggest that working status and industrial accidents of migrant workers are very poor in Korea. Therefore, occupational safety and health policy aimed at migrant workers are needed to protect the migrant workers.

대인ㆍ대내인간 통합적 의사소통 프로그램 개발

최태산,양정남 동신대학교 2005 論文集 Vol.15 No.-

연구는 대인, 대내인간 통합적 의사소통 훈련 프로그램을 개발하기 위하여 실시되었다. 연구목적을 달성하기 위해 먼저 선행연구와 임상경험을 토대로 프로그램을 구성하였다. 구성된 프로그램의 효과는 동신대학교 남·여 대학생 30명을 실험집단과 통제집단으로 구분한 후, 총 24시간(6X4)의 프로그램을 실시하여 사전-사후간의 차이를 비교, 검증하였다. 측정도구는 문제해결에 대한 자기평가검사와 자아존중감검사를 사용하였는데, 결과를 요약하면 다음과 같다. 첫째, 대인간·대내인간 통합적 의사소통 훈련 프로그램이 지각된 문제해결능력에 미치는 효과를 검증한 결과, 유의수준 .001에서 통계적으로 유의미한 차이가 있었다. 둘째, 대내인간 통합적 의사소통 훈련 프로그램이 자아존중감에 미치는 효과를 검증한 결과, 유의수준 .001에서 통계적으로 유의미한 차이가 있었다. 따라서 대인간·대내인간 통합적 의사소통 훈련 프로그램은 문제해결능력과 자아존중감 향상에 효과가 있다고 할 수 있다. This study has a goal to develop of the Integrated(Inter·Intra) Communication Training Program. The Integrated(Inter·Intra) Communication Training Program based on preceding research and clinical experience was developed. The subject of this study were 30 Dongsin university students. They divided into experimental group and control group, and then experienced 24 hours program. to examine the effects of Measurement instruments were Problem Solving Inventory and Self-Esteem Inventory. The results are as follow: 1) The results of The Integrated(Inter·Intra) Communication Training Program at a problem solving ability is revealed on significant difference(p<.001). 2) The results of The Integrated(Inter·Intra) Communication Training Program at increasing of self-esteem is revealed on significant difference(p<.001). For that reason The Integrated(Inter·Intra) Communication Training Program is effective to problem solving ability and increasing of self-esteem.

결핵균 단세포군항체에 의한 항산균항원의 면역화학적 분석에 관한 연구

최대경,백태현,박정규,김화중 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

β-antigen is one of the major protein of Mycobacterium tuberculosis. We purified reference β-antigen from the unheated culture filtrate of Mycobacterium tuberculosis by serial ammonium sulfate precipitation, gel filtration on Sephacryl S-200 column and preparative polyacryamide gel electrophoresis. Nine monoclonal antibodies against β-antigen were produced by hybridoma technique. The nine monoclonal antibodies were divided into two groups according to their reaction patterns on Western blots. Four monoclonal antibodies(group Ⅰ) reacted with two major bands of 83 and 52 kDa, and two minor bands of 110 and 56 kDa molecules while the other five monoclonal antibodies(group Ⅱ) reacted with a major band of 83 kDa and a minor and of 110 kDa molecule. The interspecies reactivities of nine monoclonal antibodies on the sonicated antigens from 21 species of mycobacteria were also examined by dot blots. Two monoclonal antibodies, designated 4G5E10(group Ⅰ) and 5F3F2(group Ⅱ), showed a specificity restricted to Mycobacterium tuberculosis complex. β-antigien from the cell extract of Mycobacterium tuberculosis was purified by affinity chromatography with 5F3F2 monoclonal antibody and checked its molecular characterization. Affinity purified β-antigen showed two distinct bands of 83 and 52 kDa molecules on SDS-PAGE. These results indicating that β-antigen is a associated protein with molecular weights of 83 and 52 kDa, and contains at least two different specific epitopes for Mycobacterium tuberculosis complex on β- antigen molecules. Affinity purified and reference β- antigens were applied for its potential diagnostic utility for determination of LgG antibody in the cerebrospinal fluids and sera of tuberculosis patients by ELISA. The mean ELISA values of IgG antibody activities to affinity purified β-antigen at the CSF dilution of 1 : 5 were 0.866±0.607 in 12 patients with tuberculous meningitis and 0.058±0.040 in 24 controls(P<0.001). The mean ELISA values of IgG antibody activities to reference β-antigen at the serum dilution of 1 : 100 were 0.861±0.350 in 97 patients with minimal tuberculosis, 1.052±0.443 in 51 patients with moderately advanced, 1.381±0.396 in 49 patients with far advanced and 0.454±0.248 in 138 controls(P<0.01). An indirect sandwich ELISA using specific biotinylated 5F3F2 monoclonal antibody as tracer was designed to detect β-antigen in the culture filtrate of Mycobacterium tuberculosis and sputum samples from patients with pulmonary tuberculosis. The detection limit of indirect sandwich ELISA was 0.1㎍ culture filtrate/㎖. The mean ELISA values of β-antigen reactivities in sputum samples from 52 patients with pulmonary tuberculosis and 25 controls were 0.766±0.404 and 0.453±0.133(not significant).