Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

청소년 학습자 대상 한국어교육을 위한 관련 연구 동향 분석 - 비한국계 청소년을 중심으로 -

김지혜 ( Kim¸ Jihye ) 연세대학교 언어연구교육원 한국어학당 2021 외국어로서의 한국어교육 Vol.62 No.-

Along with an expanded interest in Korean, the number of adolescent Korean language learners has been increasing: as of February, 2020, about 145,000 learners in 1,635 secondary schools in 30 nations are taking Korean language classes. On top of that, if we include young people who learn Korean from online K-culture contents outside school, the number will rise more. So far, Korean language education has been mainly for immigrant youth (i.e. adolescents from foreign countries, multi-cultural families, and North Korea) and overseas adolescent Koreans. However, as many researchers now realize that systemic research on Korean language education for non-Korean adolescent is also needed, a lot of studies supported by the government are on-going. Reviewing research trends in Korean language education for the adolescent, this paper attempts to find a way that Korean language education for adolescent KFL learners would move forward. For this, 218 papers published in the last twenty years were reviewed, and 35 of them, the topics of which are adolescent KFL learners, are closely analyzed. As a result, it is turned out that research for practical curricula and materials for non-Korean adolescent Korean language learners is required. The primary research here means language knowledge (i.e. vocabulary, grammar, and pronunciation), the four language skills, language development, and the analysis of affective variables. (Seoul National University of Science and Technology)

공격적 ‘여성’ 중심주의의 사회적 맥락과 전개 양상에 관한 연구

김지혜 ( Kim¸ Jihye ) 부산대학교 여성연구소 2021 여성학연구 Vol.31 No.2

본 논문은 2016년 페미니즘의 재부상 이후, 공격적 ‘여성’ 중심주의(aggressive ‘female’-centrism)가 디지털-네이티브(native) 청년 여성 세대에게 대중화된 원인과 사회적 맥락을 검토하면서 i세대 주류 페미니즘의 주요 특징들을 비판적으로 고찰한다. i세대 여성들은 사회적으로 만연한 성폭력과 디지털 성폭력 사건들을 목도하면서, 한국 남성 전체에 대한 극도의 적대감을 표출하며 미러링(mirroring)-‘렏펨’(‘radical feminism’) 정치학을 전개했다. 호모 에코노미쿠스 주체로서 젊은 여성들은 생존경쟁의 불안 속에서 사회구조적 변혁보다는 개인의 성공과 자기 역량 강화 (self-empowerment)를 지향하는 신자유주의적 페미니즘을 추구한다. 그들은 안전할 권리를 주장하며, 타자/차이를 거부하고 사회적 소수자에 대한 차별을 정당화하기도 한다. 또한, i세대의 공격적 ‘여성’ 중심주의가 온라인을 중심으로 확산되면서 디지털 문화의 부정적인 측면은 페미니즘 담론과 실천을 제한하는 요소가 되었다. 속도와 효율성을 중시하는 온라인 페미니즘은 정보의 파급력이나 젠더 의제의 활성화라는 장점 이면에 확증 편향과 편향 동화, 반지성적 극단주의, 혐오 발화 등의 문제도 초래했다. i세대 여성들의 분노는 젠더 감수성을 계발하고, 페미니스트 의식을 고양하는 정치적 정동으로 기능했다. 그러나 공격적 ‘여성’ 중심주의가 강조하는 피해자 정치학은 젠더를 ‘생물학적’ 성으로 환원하는 젠더 본질주의와 배타적 부족주의의 한계를 드러낸다. This paper examines the main factors of mainstream i-generation feminism by investigating the causes and social contexts in which aggressive ‘female’-centrism became popular among digital-native young women after the re-emergence of feminism in 2016. Witnessing the widespread sexual violence and digital sex crimes, this generation of women has expressed hostility towards all Korean men and developed radical feminist politics and tactics of mirroring. These women, as homo economicus subjects, pursue neoliberal feminism that promotes individual success and self-empowerment rather than social structural reforms amidst their precarious survival. They justify discrimination against social minorities and rejection of others and differences by bringing security to the fore. With aggressive feminism of i-generation mainly distributed and spread online, negative aspects of digital culture have become the factors that restrict feminist discourse and practice. Online feminism, which emphasizes speed and efficiency, may generate information ripples and activate gender agendas, but also causes mass production of confirmation bias, biased assimilation, anti-intellectual extremism, and hate speech. The anger of i-generation women serves as the political affect that develops gender sensitivity and raises feminist consciousness. However, the politics of victimhood asserted by aggressive ‘female’-centrism prompts the error of gender-essentialism and tribalism that reduces gender to biological sex.

노년 세대를 위한 서사 교육의 설계와 방향

김지혜 ( Kim¸ Jihye ) 한국독서학회 2021 독서연구 Vol.- No.60

본 연구에서는 신체적, 정신적, 사회적 위기감을 극복하고 삶의 질을 높일 수 있도록 하는 노년 세대를 위한 서사 교육의 방안을 모색하고자 하였다. 먼저, 노년 세대를 위한 서사 교육의 이론적 토대를 설정하기 위해 노년 정체성의 특성과 폴 리쾨르(P. Ricoeur)의 이야기 정체성 이론을 살펴보았으며, 2018년 국립국어원의 <어르신 대상 국어문화 프로그램 개발 및 시범 적용>의 서사 부분 프로그램의 설계 및 내용을 소개함으로써 노인을 위한 서사 교육의 방향을 고찰하였다. 리쾨르의 서술적 정체성(narrative identity)에 따르면 서사는 자신의 삶을 이해하고, 그 이야기를 형상화하여 공유함으로써 새로운 자기 이해를 이룰 수 있게 한다. 그러므로 노년 세대를 위한 서사 교육은 노년기의 정체성을 재확립하고, 자아존중감을 높이는 데 중요한 역할을 할 수 있다. 2018년 개발된 국립국어원의 <어르신 대상 국어문화 프로그램 개발 및 시범적용>의 서사 교육 프로그램은 서사를 통해 노년의 삶에 대한 긍정적인 인식을 확립함으로써 자아존중감을 높이고 나아가 노년기의 삶의 질을 향상시키는 것을 목표로 삼았다. 이 프로그램의 내용은 크게 네 가지 단계로 구성되었는데, 첫 번째 단계는 ‘‘나’를 돌아보기’ 단계, 두 번째 단계는 ‘노년과 마주하기’, 세 번째 단계는 ‘행복한 노년의 삶 설계하기’, 네 번째 단계는 ‘‘나’를 표현하기’이다. 본 프로그램에서는 서사를 매개로 ‘과거-현재-미래’라는 연속적인 삶의 서사를 의미화하고 이를 표현함으로써 자신의 정체성을 긍정적으로 재정립할 수 있도록 하였다. 또한 본 연구에서는 서사 교육 프로그램의 시범 강의와 만족도 조사 결과를 통해 향후 노년 세대 대상 서사 교육의 긍정적 역할을 기대할 수 있었다. 향후 노년 세대를 위한 서사 교육은 폭넓은 노년 계층을 대상으로 한 다양한 교육, 여러 세대가 함께할 수 있는 쌍방향적 교육, 다양한 매체를 활용한 다각적인 교육이라는 방향성을 고려하여 발전해야 할 것이다. This study sought a narrative education plan designed for the elderly generation allowing them to overcome physical, mental, and social crisis and improve their quality of life. To establish the theoretical base of old age identity and narrative education, this study examined the theory of narrative identity by Paul Ricoeur. Meanwhile, it considered narrative education plans for the elderly by presenting the design and contents of a narrative program entitled “Development of programs of Korean language culture and pilot application oriented toward the elderly” developed by the National Institute of Korean Language in 2018. According to the narrative identity of Paul Ricoeur, the narrative helps one attain self-understanding by allowing one to understand and share one’s life through the embodiment of the story. Therefore, narrative education for the elderly generation can be instrumental in helping the elderly discover their old age identity and increase their self-esteem. The “Development of programs of Korean language culture and pilot application oriented toward the elderly,” developed by the National Institute of Korean Language in 2018, aimed to improve the self-esteem of the elderly by establishing a positive awareness of old age life through narratives. This program largely consists of four stages: “looking back at myself,” “facing old age,” “designing a happy old age life,” and “expressing myself. The first stage of “looking back at myself,” the second stage of “facing old age,” the third stage of “designing a happy old age life,” and the fourth stage of “expressing myself.” Through awareness of “past-present-future” based on these stages, this program allows the elderly to express and reflect on themselves. Future narrative education for the elderly will be required to develop multifarious education oriented toward a range of elderly groups, interactive education, and multidirectional education applying diverse media which various generations can experience together.

SSP 기후변화 시나리오에 따른 농업용 저수지 홍수조절능력 분석

김지혜 ( Kim¸ Jihye ),곽지혜 ( Kwak¸ Jihye ),황순호 ( Hwang¸ Soonho ),전상민 ( Jun¸ Sang Min ),이성학 ( Lee¸ Sunghack ),이재남 ( Lee¸ Jae Nam ),강문성 ( Kang¸ Moon Seong ) 한국농공학회 2021 한국농공학회논문집 Vol.63 No.5

The objective of this study was to evaluate the flood control capacity of the agricultural reservoir based on state-of-the-art climate change scenario - SSP (Shared Socioeconomic Pathways). 18 agricultural reservoirs were selected as the study sites, and future rainfall data based on SSP scenario provided by CMIP6 (Coupled Model Intercomparison Project 6) was applied to analyze the impact of climate change. The frequency analysis module, the rainfallrunoff module, the reservoir operation module, and their linkage system were built and applied to simulate probable rainfall, maximum inflow, maximum outflow, and maximum water level of the reservoirs. And the maximum values were compared with the design values, such as design flood of reservoirs, design flood of direct downstream, and top of dam elevation, respectively. According to whether or not the maximum values exceed each design value, cases were divided into eight categories; I-O-H, I-O, I-H, I, O-H, O, H, X. Probable rainfall (200-yr frequency, 12-h duration) for observed data (1973∼2020) was a maximum of 445.2 mm and increased to 619.1∼1,359.7 mm in the future (2011∼2100). For the present, 61.1% of the reservoirs corresponded to I-O, which means the reservoirs have sufficient capacity to discharge large inflow; however, there is a risk of overflowing downstream due to excessive outflow. For the future, six reservoirs (Idong, Baekgok, Yedang, Tapjung, Naju, Jangsung) were changed from I-O to I-O-H, which means inflow increases beyond the discharge capacity due to climate change, and there is a risk of collapse due to dam overflow.

Dynamic and coordinated single-molecular interactions at TM4SF5-enriched microdomains guide invasive behaviors in 2- and 3-dimensional environments

Kim, Hye-Jin,Kwon, Sojung,Nam, Seo Hee,Jung, Jae Woo,Kang, Minkyung,Ryu, Jihye,Kim, Ji Eon,Cheong, Jin-Gyu,Cho, Chang Yun,Kim, Somi,Song, Dae-Geun,Kim, Yong-Nyun,Kim, Tai Young,Jung, Min-Kyo,Lee, Kyun The Federation of American Societies for Experimen 2017 The FASEB Journal Vol.31 No.4

<P>Membrane proteins sense extracellular cues and transduce intracellular signaling to coordinate directionality and speed during cellular migration. They are often localized to specific regions, as with lipid rafts or tetraspanin-enriched microdomains; however, the dynamic interactions of tetraspanins with diverse receptors within tetraspanin-enriched microdomains on cellular surfaces remain largely unexplored. Here, we investigated effects of tetraspan(in) TM4SF5 (transmembrane 4 L6 family member 5)-enriched microdomains (T5ERMs) on the directionality of cell migration. Physical association of TM4SF5 with epidermal growth factor receptor (EGFR) and integrin alpha 5 was visualized by live fluorescence cross-correlation spectroscopy and higher-resolution microscopy at the leading edge of migratory cells, presumably forming TM4SF5-enriched microdomains. Whereas TM4SF5 and EGFR colocalized at themigrating leading region more than at the rear, TM4SF5 and integrin a5 colocalized evenly throughout cells. Cholesterol depletion and disruption in TM4SF5 post-translational modifications, including N-glycosylation and palmitoylation, altered TM4SF5 interactions and cellular localization, which led to less cellular migration speed and directionality in 2-or 3-dimensional conditions. TM4SF5 controlled directional cell migration and invasion, and importantly, these TM4SF5 functions were dependent on cholesterol, TM4SF5 post-translational modifications, and EGFR and integrin alpha 5 activity. Altogether, we showed that TM4SF5 dynamically interacted with EGFR and integrin a5 in migratory cells to control directionality and invasion.-Kim, H.-J., Kwon, S., Nam, S. H., Jung, J. W., Kang, M., Ryu, J., Kim, J. E., Cheong, J.-G., Cho, C. Y., Kim, S., Song, D.-G., Kim, Y.-N., Kim, T. Y., Jung, M.-K., Lee, K.-M., Pack, C.-G., Lee, J. W. Dynamic and coordinated single-molecular interactions at TM4SF5-enriched microdomains guide invasive behaviors in 2-and 3-dimensional environments. FASEB J. 31, 1461-1481 (2017). www.fasebj.org</P>

The Stress-Reducing Effects of Forest Healing Activities in Maladjusted Military Force Members

Jihye Kim,Changseob Sin,Jihye Kim,Dohyeong Kim,Yunsu Kim,Hyunchae Lee 인간식물환경학회 2020 인간식물환경학회지 Vol.23 No.3

Background and objective: In the Republic of Korea, military service is mandatory. Some of new recruits have the stress from the special environment, which could cause psychological maladjustment. The military forces have operated education programs such as green camp and healing camp. The study was conducted to investigate changes in psychological and physiological stress by conducting forest healing activities along with plant scent treatment for soldiers participating in a green camp. Methods: A total of 52 soldiers were participated including maladjustment soldiers and those recommended by their military units to protect the unfit soldiers who participated in forest healing activities in the green camp. The programs that were certified by the Korea Forest Service between 2014 and 2019, and were applied for stress reduction and relaxation training were classified into stress-coping programs and forest experience programs. Post-hoc surveys and cortisol measurements were carried out. Results: Green camp soldiers experience and differences in the level of stress responses were found to be very statistically significant between the treatment and control groups. The techniques for coping with stress were not significantly different in the control group, and the treatment group showed statistically significant results. In addition, the results of analyzing changes in the concentration of cortisol and measuring physiological stress were very statistically significant in forest healing activities at 4 p.m. Conclusion: Once green camp soldiers face stressful situations, forest healing programs using forest plant scents for green camp soldiers can have positive responses and forest healing activities can reduce psychological and physiological stress responses, improving maladjustment behaviors caused by stress and positively affecting the reduction of cortisol.

Inhibition of VEGF ‐dependent angiogenesis and tumor angiogenesis by an optimized antibody targeting CLEC 14a

Kim, Taek‐,Keun,Park, Chang Sik,Jang, Jihye,Kim, Mi Ra,Na, Hee‐,Jun,Lee, Kangseung,Kim, Hyun Jung,Heo, Kyun,Yoo, Byong Chul,Kim, Young‐,Myeong,Lee, Je‐,Wook,Kim, Su Jin,Kim, Eu John Wiley and Sons Inc. 2018 MOLECULAR ONCOLOGY Vol.12 No.3

<P>The C‐type lectin‐like domain of CLEC14a (CLEC14a‐C‐type lectin‐like domain [CTLD]) is a key domain that mediates endothelial cell–cell contacts in angiogenesis. However, the role of CLEC14a‐CTLD in pathological angiogenesis has not yet been clearly elucidated. In this study, through complementarity‐determining region grafting, consecutive deglycosylation, and functional isolation, we generated a novel anti‐angiogenic human monoclonal antibody that specifically targets CLEC14a‐CTLD and that shows improved stability and homogeneity relative to the parental antibody. We found that this antibody directly inhibits CLEC14a‐CTLD‐mediated endothelial cell–cell contact and simultaneously downregulates expression of CLEC14a on the surface of endothelial cells. Using various <I>in vitro</I> and <I>in vivo</I> functional assays, we demonstrated that this antibody effectively suppresses vascular endothelial growth factor (VEGF)‐dependent angiogenesis and tumor angiogenesis of SNU182 human hepatocellular carcinoma, CFPAC‐1 human pancreatic cancer, and U87 human glioma cells. Furthermore, we also found that this antibody significantly inhibits tumor angiogenesis of HCT116 and bevacizumab‐adapted HCT116 human colorectal cancer cells. These findings suggest that antibody targeting of CLEC14a‐CTLD has the potential to suppress VEGF‐dependent angiogenesis and tumor angiogenesis and that CLEC14a‐CTLD may be a novel anti‐angiogenic target for VEGF‐dependent angiogenesis and tumor angiogenesis.</P>

Physiological and psychological effects of forest healing focused on plant fragrance therapy for maladjusted soldiers

김지혜(Jihye Kim),신창섭(Changseob Sin),박종옥(Jong-ok Park),이현채(Hyunchae Lee),김지혜(Jihye Kim),김도형(Dohyeong Kim),김상희(Sanghee Kim) 인간식물환경학회 2021 인간식물환경학회지 Vol.24 No.4

Background and objective: This study verified the physiological and psychological effects of plant fragrance therapy to analyze whether the therapy has a forest healing effect on maladjusted soldiers. Methods: This study was conducted from March 14 to May 31, 2020 according to the Green Camp curriculum for 2 weeks per recruit class. Thus, plant fragrance therapy was implemented twice each for three recruit classes. Each session of the program was carried out for 4 hours from 1 p.m. to 5 p.m. The venue was the garden within the camp and the forest nearby. Forest healing activities using plant fragrances were led by 1 forest education specialist and 1 assistant. The effects of plant fragrance therapy for maladjusted soldiers were analzed analyzed by quantifying physiological and psychological changes through measuring brain waves and stress levels before and after the therapy. Results: As a result of analyzing BRQ and SQ to determine the physiological and psychological healing effect of plant fragrance therapy, both the left and right brain showed higher BRQ and SQ after the therapy with statistical significance. This indicates that plant fragrance therapy on maladjusted soldiers helped positively stabilize their negative psychological and behavioral dispositions, and stabilization of brain waves lowered physical and mental stress and improved self-regulation and immunity. On the other hand, there was no significant difference in ATO. This is the result of analyzing the stress-coping mechanism. Troubleshooting (t = -2.702, df = 61, p = .009), emotional remission (t = -2.173, df = 61, p = .034), pursuit of help (t = -3.286), df = 61, p = .002), and wishful thinking (t = -3.714, df = 61, p < .001) showed statistically significant results. Conclusion: Applying plant fragrance therapy to maladjusted soldiers positively stabilized their negative psychological and behavioral dispositions and helped their self-regulation. Thus, plant fragrance therapy has psychological and physical forest healing effect on maladjusted soldiers.

Effects of Long-acting Injectable 3-Monthly Paliperidone Palmitate on the Clinical and Social Performance of Patients with Schizophrenia

Jihoon Oh(Jihoon Oh),Jihye Oh(Jihye Oh),Dong Wook Kim(Dong Wook Kim),HyunChul Youn(HyunChul Youn),Sae-Hoon Kim(Sae-Hoon Kim),Soo In Kim(Soo In Kim),In Won Chung(In Won Chung),Kuan Shu Wang(Kuan Shu Wa 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.1

Objective: To investigate the effects of long-acting injectable 3-monthly paliperidone palmitate on the clinical and social functioning of patients with schizophrenia. Methods: This study enrolled patients with schizophrenia receiving long-acting injectable 1-monthly paliperidone palmitate for at least 4 months and who subsequently received 3-monthly paliperidone palmitate. Accordingly, 418 patients were followed up for 24 weeks. Their clinical symptoms and social functioning were measured using the Clinical Global Impression-Severity of Illness and Personal and Social Performance scales. Results: The Personal and Social Performance total score was significantly higher after 3-monthly paliperidone palmitate treatment than at baseline (baseline vs. week 24: 54.3 ± 18.0 vs. 61.0 ± 14.5 [mean ± standard deviation]; p < 0.001; Wilcoxon signed-rank test); the proportion of patients in the mildly ill group (scores 71−100) also increased significantly (baseline vs. week 24: 16.5% vs. 20.6%; p < 0.001; McNemar-Bowker test). The mean Clinical Global Impression-Severity of Illness score decreased significantly (baseline vs. week 24: 3.7 ± 1.0 vs. 3.4 ± 0.9; p < 0.001; Wilcoxon signed-rank test), as did the proportion of patients in the severely ill group (baseline vs. week 24: 4.1% vs. 2.1%; p < 0.001; McNemar-Bowker test). Conclusion: Continuous 3-monthly paliperidone palmitate treatment significantly enhances the personal and social performance of patients with schizophrenia and reduces the proportion of those with severe illness. These findings suggest that long-acting injectable antipsychotic administration at intervals longer than 1 month might improve the social functioning of and promote return to activities of daily living in patients with schizophrenia.