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들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향
이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.
서영범,김성욱,장재식,강혁주,이중현,윤병구,김욱년,이광헌,이구,유석동,양창헌,이정호,이영현,이창우,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-
자가면역성 간염은 대개 만성 경과를 가지며, 혈중 자가면역항체와 혈청 글로불린치의 상승, 그리고 조직학적으로 괴사 염증성 변화를 특징으로 하는 질환으로 아직 정확한 병인이 밝혀져 있지 않은 상태이다. 발병연령은 대개 젊은 영자(15-25세)에서 호발한다. 이 질환은 급성 간염의 임상경과를 보일 수 있으나, 심한 급성 간염이나 전격성 간염으로도 나타날 수 있는데, 이 경우 아주 나쁜 예후를 보인다고 한다. 치료는 자가면역성 간염 임상 경과의 다양성이나 병인, 병리기전의 불확실성에도 불구하고 대개 steroid 치료에 반응하는 것으로 알려져 있으며 대개 80%의 관해율을 나타내며 궁극적으로 간경변으로의 진행을 막을 수 있는 것으로 보인다. 저자들은 58세 남자에서 발생한 급성의 경과를 가지고 심한 황달을 동반한 자가면역성 간염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Autoimmune hepatitis (AIH) is a chronic necroinflammatory liver disorder of unknown cause associated with circulating autoantibodies and a high serum globulin level. The age of onset of AIH show a peak between the age of 15 and 25 years. AIH can develop and be manifested as acute hepatitis, but severe form of acute hepatitis or fulminant hepatic failure has a poor prognosis. Although AIH is likely to progress from chronic active hepatitis to cirrhosis, steroid therapy can control the disease activity, prolong survival, improve the quality of life , and defer liver transplantation. In the present report we describe a 58-year-old man who admitted because of progressive jaundice and fatigue. He was diagnosed with AIH from laboratory test result showing positivity for antinuclear antibodies, anti-smooth muscle antibodies, and negativity for hepatitis viral markers and from liver biopsy. Steroid therapy, oral administration of prednisolone, was effective in improving the liver function test. Following liver biopsy 6 months after onset shows markedly improved necroinflammatory activity.
서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)
김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1
In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.
GC/MS-SIM과 ³²P-postlabeling방법에 의한 염료와안료를 제조하는 사업장 근로자들의 방광암 조기발견을 위한 Biomarker개발에 관한 연구
이진선,신호상,김양호,노재훈,이범규 공주대학교 자원재활용신소재지역협력센터 2000 2차년도 센터 사업 성과집 Vol.2000 No.1
To develop the biomarker of non-invasive biomonitoring among workers who exposed benzidine-based dyes in its manufacturing and treating industries, we examined and evaluated the specific carciaogen-DNA adducts in exfoliated urothelial cells of 33 exposed workers. Samples were collected from urine of workers, and then isolated DNA from them. DNA adducts were analyzed using 32P-postlabeling, along with Nuclease P1. One adducts was predominated in those samples with 6 adducts present. It was formed in all exposed workers, but the others not. Its relative adducts level(RAL) was 73.2×107, which was statistically significantly higher than any other adducts' level(p<0.05). Analyzing regression between its RAL and employment period of workers, simple linear regression was positively increased with slop of 6.922(p<0.05, r2=0.548). And also, DNA adducts were analyzed using GC/MS-SIM, along with n-butanol extraction and trifluoroacetyl auhydride(TFA) derivatization. Standard material was synthetic N-(deoxyguanosin-8-yl)-N'-acetylbenzidine. The characteristics of sample and standard were observed on the same sites, such as M+(m/z 376) and M+(m/z 279) for benzidine-di-TFA and mono-TFA. And then, they were confirmed by analyzing with secondary ion mass(SIM). This study showed that benzidine-based dyes was formed one DNA adduct into significantly high levels in bladder of all exposed workers, its chemical formula was N-(deoxyguanosin-8-yl)-N'-acetylbenzidine. So we can do monitoring the workers with exfoliated urothelial cells of them by analyzing above DNA adduct as biomarker for preventing bladder cancer causing the exposure of the benzidine-based dyes.
편평세포폐암에서 p53 돌연변이의 발현과 TNM 병기와의 상관관계
이상숙,한승범,전영준 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4
Primary lung cancer is now recognized as a major cause of cancer death in Korea as well as the whole world. Non-small-cell lung cancers(NSCLC) comprise about 75% of lung cancer and squamous cell carcinoma is the most common type in Korea. Mutations of p53 gene are common in variety of human cancers, including lung cancer. The p53 gene appears to inhibit the proliferation of cells from the G1 to the S phase of cell cycle and is able to suppress the transformations of cells by other oncogenes, to inhibit the growth of malignant cells in vitro and suppress the tumorigenic phenotype of transformed cells. Alteration or inactivation of p53 by mutation, or by its interactions with oncogene products of DNA tumor viruses, can lead to cancer. These mutations seems to be the most common genetic changes in human cancers. p53 gene mutation is known to be a poor prognostic marker in breast cancer and has significant association with lymph node involvement. But in human lung cancer the association of p53 mutations and TNM stages are controversial. Immunohistochemical staining can detect only mutant p53 protein bacause of markedly prolonged half life of mutant p53 protein. We performed the immunohistoschemical staining on 48 surgically resected and 10 bronchoscopically biopsized specimens of primary squamous cell carcinoma of the lung with monoclonal antibody(DO 7) and analyzed the relationship between the expression of p53 protein and clinical TNM stages. p53 protein was detected in 60.3% of total 58 cases. p53 protein was positive in 52.9% of stage I(17cases), 83.3% of stage Ⅱ(6 cases), 72.2% of ⅢA(18 cases), 42.9% of ⅢB(7 cases) and 50% of stage Ⅳ(10 cases) which revealed no significant correlation between p53 protein detection and stages. There was no significant correlation between p53 protien detection and No and N₁-₃as well Mo and M₁. In conclusion, we couldn't find any significant correlation between p53 protein detection and TNM stages which suggests p53 mutation is frequent in squamous cell carcinoma of the lung but play different role in progression of clinical stages.
이형호,정성범 韓國福祉行政學會 2002 복지행정논총 Vol.12 No.1
본 연구는 지방자치제 실시 이후 지방정부들의 복지실태와 복지수요, 그리고 복지재정의 문제점과 복지재정의 확충방안을 목적으로 하고 있다. 범위로는 공공복지만을 대상으로 했으며 방법은 기존의 관련 문헌을 기초로 하였다. 이론적 배경으로 지방복지재정의 개념과 구성, 그리고 복지재원조달의 기본원칙과 지방정부의 복지수요, 지방정부와 지역복지와의 관계, 지방정부복지재정의 현황을 살펴보았다. 그리고 지방정부의 복지재정의 문제점을 복지전달체계상의 문제점, 지방정부 재정조정제도상의 문제점, 복지재정예산의 총체적 부족과 경직성, 수익자부담원칙에의 과도한 의존으로 지적하였고, 복지재정의 확충방안으로 내부조정에 의한 복지재정의 확충, 조세에 의한 복지재정의 확충, 행정기능의 재조정과 복지기능의 효율화로 복지재정의 확충, 생산적복지를 통한 복지비 지출의 감소, 공공복지지출체계의 효율화로 인한 복지재정의 확충 등으로 하였다.