직접 검화법을 이용한 조제분유의 콜레스테롤 분석법 개발

김진만,박정민,윤태형,임동길,윤창용,정자영,정인식,곽병만,안장혁,Kim, Jin-Man,Park, Jung-Min,Yoon, Tae-Hyung,Leem, Dong-Gil,Yoon, Chang-Yong,Jeong, Ja-Young,Jeong, In-Seek,Kwak, Byung-Man,Ahn, Jang-Hyuk 한국축산식품학회 2011 한국축산식품학회지 Vol.31 No.6

본 연구에서는 조제분유 중의 콜레스테롤을 신속하고 정확하게 분석하기 위해 지방 추출 과정을 거치지 않는 직접 검화 방법을 선택하여 분석법 개발을 시도하였다. 조제분유 분말시료를 직접 검화 수기에 취하여 검화 온도, 검화 시간, KOH 농도의 3가지 인자에 대해 콜레스테롤 회수율이 가장 양호하게 나타나는 최적 검화조건을 확립하고, 검화 후 수세과정에서 액액 분배가 용이한 용매 조건도 확립하였다. 또한 콜레스테롤 피크의 완전한 분리를 위한 적정 기기 조건을 확립하였다. 그 결과, 시료 약 2 g에 16 M-KOH 10 mL를 넣고 $90^{\circ}C$에서 60분 가열하여 검화한 후 diethyl ether로 3회 추출하고 hexane을 최종시험용액으로 하여 기기분석을 했을 때의 회수율이 98.80%로서 가장 양호하게 나타났다. 본 연구를 통해 개발된 조제분유의 효율적인 액액분배 및 직접가열 검화법은 일원배치 분산법에 의해 유화가공식품의 콜레스테롤 분석법으로 유효성이 검증되었으며, 아울러 개발된 전처리 방법 및 기기 분석 조건을 활용해 다양한 분석 기관에서 신속 정확하고 효율적인 콜레스테롤 분석을 수행할 수 있을 것으로 기대된다. 또한 산업체의 품질관리 및 검증기관에서 필요시 모니터링에 적극 활용 가능할 것으로 사료되며, 이를 통해 유화가공식품 류의 함량표시 및 규격관리의 정확성과 효율성 증대에 기여하여 제조업체의 정확하고도 효과적인 품질 및 안전성 확보에 기여할 수 있을 것으로 판단된다. 특히 위해 물질에 대한 정확한 함량 판단이 중요한 조제분유 등의 영 유아용 식품의 안전성 확보에 지대한 공헌을 할 수 있을 것으로 기대된다. An improved cholesterol analysis method was developed for powdered infant formula by gas chromatographic separation after liquid-liquid extraction and partition. In the official Korea Food Standard method for cholesterol analysis, the water phase and solvent phase were not well separated in the case of emulsified foods such as powdered infant formulas and baby foods. For the rapid and simple sample preparation method, an optimized direct saponification condition was established for heating temperature, heating time, and KOH concentration. From the results, the optimum conditions were as follows: heating temperature $90^{\circ}C$, heating time 60 min, and 16 M KOH 10 mL for a 2 g infant formula sample; improved separation condition for gas chromatography was as follows: the initial oven condition was $250^{\circ}C$ for 25 min, the oven temperature was increased to $290^{\circ}C$ by $10^{\circ}C$/min ratio, and finally the oven temperature remained at $290^{\circ}C$for 9 min. The developed method could be implemented for the study of cholesterol, providing the advantages of reduced inspection time and cost in emulsified foods such as infant formula.

다량 조리시 조리 방법에 따른 상용 채소류의 비타민 B_1, B_2, C 및 무기질 함량의 변화

장명숙,서민자,김나영 단국대학교 1998 論文集 Vol.32 No.-

The contents variation of vitamin B_1, B_2, C and mineral components of daily vegetables by cooking methods in the quantity food preparation was investigated. Because various nutrients change during food preparation, it is important to estimate nutrient contents of food items as served. Many kinds of daily vegetables served in the hospital during two months was analyzed for this study. 8 kinds of different cooking methods including seasoned fresh vegetables, seasoned blanched vegetables, deep-fat fried vegetables, pan fried vegetables, steamed vegetables, braised vegetables etc, were applied to 37 vegetables items. And the vegetables analyzed were classified as four groups : leafy vegetables, root vegetables, fruit vegetables, and the other vegetables As a result of this study, all of the four vegetables groups showed same tendency that most of the nutrients content were various by cooking methods in spite of same vegetable item. In the leafy vegetables group, moisture content of seasoned fresh vegetables were higher than seasoned fresh vegetables. In the root vegetables group, crude ash content among the cooked radish root showed the highest value in the seasoned fresh radish root. Vitamin B_1 contents were the highest in the seasoned fresh radish root, pan-fried onion comparing to the same vegetable cooked by any other cooking methods. Vitamin C contents had the same currency seasoned fresh radish root and Doraji, deep-fat fried onion. In the fruit vegetables, crude protein content of seasoned fresh green red pepper was the highest in the middle of cooked green red pepper. In the case of pumpkin, immature the highest vitamin B_1 content was observed in the pan-fried pumpkin, immature but that of vitamin B_2 was observed in the saute fresh pumpkin, immature. Seasoned fresh cucumber with hot pepperpaste had the highest contents in most mineral components excepting iron, respectively. Finally, the other vegetables group analysed was consist of dishes cooked with more than two kinds of vegetables or vegetables which couldn't be included in the previous classification. Therefore, the nutrients content of cooked daily vegetables acturally served in the hospital were investigated. Also, there were differences of nutrient contents by various cooking method in spite of same vegetables item under the condition for this study. Furthermore, it would be desirable that investigation about comparison of nutrient contents between quantity food preparation and home made cooking carried out.

동적 채널 할당을 윈한 무선 중계 시스템

장자영,노순국,김송민,김재섭,허영팔,박창균 조선대학교 동력자원연구소 1997 動力資源硏究所誌 Vol.19 No.1

Being adopted the Cellular Systems in Korea, the method of a radio repeater is that the mobile station(MS) can be used only in a service area of the base station(BS). In case that traffic congestion is occured around the specific BS because of an event etc., the traffic blocking rates are being maximized and the subscriber service is being dropped. To improve these problems, we propose an algorithm for a radio repeater network that distributes the traffics of the specific BS, predicted traffic congestion, to an adjacent BS by sharing a service area. And then, we confirm the valid through the simulation.

혈당검사 방법 별 지각된 통증정도에 대한 비교 : 시각적 상사 척도 이용

최자윤,장금성,김현오,최옥엽,박민희 성인간호학회 2003 성인간호학회지 Vol.15 No.1

Purpose: The purpose of this study is to determine the level of pain related to a blood sugar test. Specifically, the study attempts to compare the varying degree of pains when different types of blood sugar test are used. Method: A sample of 56 subjects is composed of DM patients admitted to a medical ward of C university hospital in Gwangju. Data were collected from July, 2001 to December, 2001. The blood sugar tests were administered in four different ways: (1) the use of 27G needle only. (2) the use of 27G needle followed by ice-packed treatment, (3) the use of 27G needle after EMLA cream application, and (4) the use of lancet. The degree of pain is measured with a visual analogue scale and performed twice. Result: in both measures, the use of 27G needle only method is shown to cause the highest level of pain in comparison with the rest of methods (F=4.01, p=.01; F=8.14, p=.00). However, the differences in pain between time in all methods were not found to be significant (t=-.85, p=.40; t=.80, p=.42; t=.31, p=.75; t=.19, p=.85). Conclusion: The study results indicate that the method using lanceter is more recommendable than the use of 27G needle only method. Further research is needed to support the current study result with the use of different measurement scales and to determine effective methods of blood sugar test to lower pain and compliance.

B형 간염예방에 대한 교육매체의 효과 : 중·고등학생을 대상으로

김매자,신계영,장애경,서민희 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: Our research team developed two types of hepatitis B prevention materials: video tape and leaflet. We showed them to two groups of subjects separately, and the effect of education was compared to find out a more effective educational material for preventing hepatitis B. Method: Students at a middle school and a high school, one class for each school year, participated in the five areas of this study. They were educated for hepatitis management using the video tape and the leaflet. Before and after the education, they were tested for knowledge on hepatitis and health belief to analyze the effect of education. Results: 1) The video tape was more effective than the leaflet in delivering hepatitis knowledge and enhancing sensitivity & severity in health belief. 2) Hepatitis knowledge was improved significantly after the education. 3) Sensitivity & severity in health belief was improved significantly after the education. 4) Benefit in health belief was improved significantly after the education 5) Barrier in health belief decreased significantly after the education. 6) There was a correlation between hepatitis knowledge, sensitivity & severity in health belief and benefit in health belief. Conclusion: We suggest that for preventing hepatitis B it would be helpful to educate students in middle school and high school face to face with educational materials.

성인 지역사회 폐렴의 원인 미생물에 대한 전향적 다기관 연구 : Legionella, Leptospira, Hantaan virus and Orientia tsutsugamushi

김민자,정희진,손장욱,심희선,박대원,박승철,우준희,강재명,김유겸,신완식,김양리,이환종,김지희 대한감염학회 2001 감염 Vol.33 No.1

Background : Despite rigorous investigations, the etiology of community-acquired pneumonia remains unknown in about 50% of hospitalized patients. The diagnosis of the etiological agent is becoming more challenging and more critical as number of newer pathogens have been recognized in recent years. In the 3-year period prospective study we investigated adult patients with community-acquired pneumonia for Legionella, Leptospira, Hantaan virus and Orientia tsutsugamushi as potential etiologic agents. Methods : A prospective multicenter study was performed from May 1997 to April 2000. A total of 431 patients with community-acquired pneumonia under the inclusion criteria were examined for specific microbial diagnosis; sputum culture and PCR, and serologic teats including indirect immunofluorescence antibody (IFA) test for Legionella, and hemagglutination tests for Leptosoira, Hantaan virus and O. tsutsugamushi. Etiologic diagnosis was determined on the basis of the review of case record forms and specific laboratory diagnostic criteria. Results : During the study period a total of 385 sputum and 283 serum samples were examined. Legionella pneumonia was diagnosed in 2.3% (10/431) of the cases examined : 1.4% cases with PCR-positive (5/367) and 2.1% with positive IFA test (6/283). Leptospirosis and scrub typhus were diagnosed in 0.4% (1/252) and 2.0% (5/252), respectively. All 5 cases with scrub typhus occurred in late fall, and rash or eschar was not found. None of cases was Hantaan virus infection. Conclusion : The results suggest that Legionella, Leptospira, and O. tsutsugamushi should be considered in the etiologic diagnosis and empirical antibiotic therapy of community-acquired pneumonia. (Korean J Infect Dis 32:24∼31, 2001)

소아 Henoch-Schonlein 신염의 임상경과와 조직학적 특성 : Renal Outcome and Prognostic Factors

김병목,장창환,조민현,곽정식,고철우,구자훈 대한신장학회 2003 대한신장학회잡지 Vol.22 No.6

서울시에서 산발적으로 분리된 Salmonella enterica Serotype Typhi 임상분리균주들의 분자역학적 분석

이희무,김철현,손장욱,김민자,박미선,이복권 대한감염학회 2000 감염 Vol.32 No.5

지속성 외래복막투석 환자들의 예후에 영향을 미치는 요인들에 관한 고찰

권혁만,김찬덕,조지형,장민화,최혁준,이현철,박자용,박선희,김용림 대한신장학회 2004 대한신장학회잡지 Vol.23 No.2

크론병에 대한 CoPPLX의 방어효과에 대한 연구

이호학,김유림,문창기,이재일,양병윤,김종수,이정민,정재혁,안석진,박상준,김윤권,김소연,김영중,조민구,최영자,장성조 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1