소방대원의 근무교대방식이 현장대응능력에 미치는 영향

심희선,정영진 한국화재소방학회 2017 한국화재소방학회 학술대회 논문집 Vol.2017 No.추계

끝없는 내리 사랑

심희선 샘터사 2001 월간 샘터 Vol.32 No.5

중·소도시 뉴타운지역 주거세입자 의식 및 심리조사 연구

심희선,권대중 대한부동산학회 2010 大韓不動産學會誌 Vol.28 No.2

This study attempted to make an empirical analysis of resident tenants’ awareness about the housing redevelopment project in its process with a focus on the new town district of Kimpo City(housing redevelopment district of the urban improvement promotion project), the small- to medium-sized city. As a result, the following findings were obtained: First, Kimpo city is the place where many low-income brackets said to be the urban needy live because of the geographical locational condition that it is located near from Seoul. It is necessary to consider the measure for livelihood of those low-income brackets as well as the measure for their migration in case the housing redevelopment project is promoted in the future. Second, the survey of their awareness of the housing redevelopment project showed that a considerable part of them were not aware of the housing redevelopment project well. Especially, 95% of the respondents never knew about the rights given to tenants, which can be said to be a big problem. Accordingly, it is necessary to provide them with education to help them understand the housing redevelopment project or the presentation meeting for migration measure along with the project presentation meeting. Third, a considerable part of the respondents said that they would not migrate to another place until proper compensation is given to them in relation to the problem occurring at the time of migration as well as the point in time of migration at the time of progressing the redevelopment project. It remains as a problem in the process of promotion in the future. Accordingly, it is necessary to induce them to understand the provisions of compensation for migration stipulated in the law sufficiently through education and public relations as long as the association does not provide them with the livelihood measure and compensation for migration additionally. Fourth, it is necessary to enable them to live in the small-scale rental apartment with low key money and monthly rent in case they move into the public rental apartment. Fifth, it was found that all of the respondents felt unsatisfied with the compensation for migration of resident tenants and the supplier of rental housing. So it is necessary to continue to study it in the future as the current law cannot be amended. 중·소도시인 김포시 뉴타운지역(도시재정비촉진사업지구 입안 중, 주택재개발지구)을 중심으로 주택재개발사업 과정에 주거 세입자들이 주택재개발사업에 대한 의식과 심리조사를 실증·분석 연구한 결과 첫째, 김포시는 서울특별시에서 근거리에 위치하는 지리적입지조건 때문에 도시빈민이라고 할 수 있는 저소득 계층이 많이 거주하고 있어 향후 주택재개발사업이 추진될 경우 저소득층인 이들에 대한 이주대책을 정부에서 마련해 두었지만 생계대책까지도 고려해 두어야 할 것이다. 둘째, 주택재개발사업에 대한 의식조사에 서 거주자의 상당부분이 재개발사업을 잘 모르고 있었으며 특히, 세입자에게 주워지는 권리에 대해 95%가 전혀 모르고 있어 역시 문제점으로 나타났다. 따라서 이들에게 주택재개발사업에 대한 이해를 돕기 위한 교육 또는 사업설명회와 아울러 이주대책 설명회 등이 마련되어 있지만 철저한 준비와 교육이 강조된다. 셋째, 재개발사업 진행시 이주시점과 이주 시 문제점에 대해서는 상당부분 세입자가 주거 이전비를 받더라도 적절한 보상이 더 이루어질 때까지 이주하지 않을 것이라고 응답해 향후 추진과정에 문제점으로 남는다. 따라서 조합에서 추가로 이들에게 생계대책이나 이주보상비를 지급하지 않는 한 법으로 정해진 이주 보상규정을 충분히 교육과 홍보를 통해 이해시킬 필요성이 있다. 넷째, 공공임대아파트에 입주할 경우 소형임대아파트를 원하고 있어 저렴한 보증금과 월세로 입주할수 있도록 해야 할 것이다. 다섯째, 주거세입자의 이전 보상비에 대한 의견과 임대주택공급대상자에 대한 의견에서 모두 불 만족한 것으로 조사되어 현행법을 고칠 수는 없으나 향후 이에 대한 연구가 계속 되어야 할 것이다.

서울시내 대형건물 냉각탑수의 레지오넬라균의 오염도 조사와 분자형별 분석에 관한 연구

김민자,김우주,김권범,박승철,유세화,함희진,박석기,심희선 대한감염학회 1998 감염 Vol.30 No.3

목적 : 레지오넬라증의 발생빈도를 낮추고 유행을 예방하기 위해서는 그 지역 환경수의 레지오넬라 오염도의 감시조사와 감염원을 찾을 수 있는 분자역학적 도구가 필요하다. 본 연구는 여름철 전 서울시내 대형건물들의 냉각탑수에서 레지오넬라균의 오염도를 조사하며, 지역별로 분포하고 있는 레지오넬라 균주의 분자형별을 분석함과 동시에 신속하면서도 재현성이 높은 분자역학적 기법을 시도하여 레지오넬라 감염의 유행적 또는 산발적 발생시에 감영원을 효율적으로 규명할 수 있는 역학적 지표를 확립하고자 하였다. 방법 : 1997년 6월 한달 간 서울시내 25개 구의 120개 대형건물의 냉각탑수를 채취하여 배양검사를 시행하고, Legionella species와 Legionella pneumophila에 특이한 duplex- polymerase chain reaction(PCR)과, 55-rRNA와 mip 유전자를 소식자로 사용하는 Southern hybridization 방법을 시행하여 레지오낼라균을 검출하여 오염도를 조사하였다. 분리된 레지오넬라 22균주를 대상으로 sfiI 제한효소를 이용한 pulsed gel electrophoresis(PFGE)와 M13 reverse primer를 이용한 arbitrarily primed(AP)-PCR을 시행하여 분리형별을 분석하였고, 두 방법간의 신속성과 분별력을 비교하였다. 결과 : 전체 120개 냉각탑수의 배양검사에서 레지오넬라균은 17개 구의 22개(18.3%) 시료에서 동정되었으며, L. pneumophila serogroup 1이 17주(77.3%), L. pneumophila serogroup 6는 4주(18.2%) 였고, 나머지 동정 미상의 Legionella가 1주였다. Duplex-PCR에서 전체 25개구의 106개(88.3%) 시료에서 레지오넬라가 검출되었고, 두 가지 방법을 모두 적용했을 때 냉각탑수는 전체적으로 90.8%(109/120)의 오염도를 나타냈었다. 분자형벌의 분석에서 17주의 L. pneumophila serogroup 1은 PFGE법에 의해 7개의 아형, 즉, A0형이 9주, A1 및 A3형이 각각 2주, A2, B, C, D형이 각각 1주로 구분되었고, L pneumophila serogroup 6의 4주는 모두 동일한 E형, 동정이 불가능했던 1주는 F형으로 구분되었다. AP-PCR법에 의해서 L. pneumophila serogroup 1은 la형이 11주, Ib 및 Ic형이 각각 2주, Ⅱ과Ⅲ형이 각각 1주로서 5개의 아형으로, L. pneumophila serogroup 6의 4주는 모두 Ⅳ형으로, 동정미상의 1주는 V형으로 구분되었다. L. pneumophila serogroup 1의 PFGE와 AP-PCR 형별의 일치율은 76.5%(13/17)였고, 전체 분리균주들의 일치율은 81.8%(18/22)였다. 분자형별의 분포는 지역적으로 다양하였고, 마포구에서 분리된 5주는 모두 다른 분자형별을 보였다. 결론 : 서울 시내 대형건물의 냉각탑수의 대부분은 이미 여름철 이전에 레지오넬라균에 오염되어 있었다. 주요 분리균인 L. pneumophila serogroup 1의 분자형별은 다양하였으며, 지역적 분포의 연관성은 없었다. AP-PCR을 이용한 레지오넬라의 분자형별 분석법은 매우 신속하고 간편하며, 비교적 분별력이 우수하고 재현성이 좋아서 레지오넬라증의 감염원 조사에 유용한 분자역학적 도구의 하나로 사료된다. Background : To prevent and control legionellosis outbreaks, it is important to monitor cooling towers for Legionella and establish epidimiological markers. We determined level of contamination with Legionella of cooling tower in Seoul city, analyzed the distribution of Legionella subtypes, and evaluated molecualar typing methods for discrimination power and feasibility. Methods : Water samples from 120 cooling towers in 25 areas(Gu) of Seoul city were collected during June, 1997. Culture and duplex-PCR(polymerase chain reaction) with Southem hybridization probed with Legionella-specific genes were performed with filtered samples. Twenty-two Legionella isolates were analyzed comparatively by pulsed-field gel electrophoresis(PFGE) and arbitrarily primed(AP)-PCR using a M13 reverse primer. Results : Culture and duplex-PCR with Southern hybridization were positive for Legionella in 22(18.3%) and 106(88.3%) of 120 samples, respectively, resulting in 90.8%(109/120) of contamination level. Out of 22 Legionella isolates, 17 were identified as Legionella pneumophila serogroup 1, 4 as L. pneumophila serogroup 6 and 1 as an unknown. Molecular analysis of 17 isolates of L. pneumophila serogroup 1 showed 7 subtypes by PFGE(A0 in 9 isolates; A1, 2; A2, 1;A3, 2; B, 1; C, 1; D, 1) and 5 subtypes by AP-PCR(la in 11 isolates; 1b, 2; 1c, 2; Ⅱ, 1; Ⅲ, 1) The agreement of results of both methods was 76.5%(13/17) of L. pneumophila serogroup 1 and 81.8%(18/22) of all isolates, respectively. Conclusion : Most of cooling towers in Seoul city were already contaminated with Legionella just before summer, requiring decontamination measures and continuous surveillance. L. pneumophila serogroup 1 was the predominant isolate with variable subtypes. The AP-PCR can be used as a rapid and reproducible screening tool in tracking legionellosis outbreak.

홍삼성분의 혈당강하작용 연구 ( Ⅱ ) : 쥐의 배양 간세포의 당대사 관련 효소 활성에 미치는 홍삼 지용성 분획의 영향 조사 ( Hypoglycemic Action of Red Ginseng Components ( Ⅱ ) : Investigation of the Effect of Fat Soluble Fraction from Red Ginseng on Enzymes Related to Glucose Metabolism in Cultured Rat Hapatocytes )

이현아,심희선,최강주,이희봉 고려인삼학회 1998 Journal of Ginseng Research Vol.22 No.1

레지오넬라 폐렴의 진단용 바이오마커의 발굴: A/J 마우스 감염 모델에서 Legionella pneumophila의 독력 유전자들의 발현양상 분석

김승민,심희선,김희남,심호기,윤영경,김정연,박윤선,박대원,손장욱,김민자 대한감염학회 2010 Infection and Chemotherapy Vol.42 No.1

Background: Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe form of pneumonia. After L. pneumophila is inhaled through contaminated aerosols, it is phagocytized by alveolar macrophages, multiplies in a specialized phagosome approximately 10 h postinfection, and eventually leads to the death of host cells. Currently available diagnostic tests for Legionella pneumonia have some limitations. This study was conducted to find diagnostic biomarkers for Legionella pneumonia using virulence gene expression profiling in a murine experimental model. Materials and Methods: A/J mice were intranasally inoculated with L. pneumophila serogroup 1, and lungs were harvested 4, 8, 24, and 48 h postinfection. The strain grown in buffered yeast extract broth was used as reference samples. Cy-dye labeled cDNA samples were prepared with total RNA from lungs or broth culture, and hybridized on the oligo-microarray slide containing 2,895 genes of L. pneumophila serogroup 1. Virulence gene expression patterns were analyzed using a MIDAS software from TIGR (www. tigr.org). Results: Among a total of 332 virulence genes examined, 17 genes including sidA, lepB, the genes related to flagella assembly (fliR and fliP), LPS lipid A biosynthesis, and the enhanced entry protein EnhA were up-regulated at all four time points. We further confirmed by quantitative real-time reverse transcription PCR that the expression of fliP gene was highly expressed in lung tissue as well as in bronchoalveolar lavage fluids from the mouse infected with L. pneumophila serogroup 1. Conclusions: Through gene expression analysis of L. pneumophila in a mouse model, several candidate biomarkers for diagnosing Legionnaires’ disease could be identified. Background: Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe form of pneumonia. After L. pneumophila is inhaled through contaminated aerosols, it is phagocytized by alveolar macrophages, multiplies in a specialized phagosome approximately 10 h postinfection, and eventually leads to the death of host cells. Currently available diagnostic tests for Legionella pneumonia have some limitations. This study was conducted to find diagnostic biomarkers for Legionella pneumonia using virulence gene expression profiling in a murine experimental model. Materials and Methods: A/J mice were intranasally inoculated with L. pneumophila serogroup 1, and lungs were harvested 4, 8, 24, and 48 h postinfection. The strain grown in buffered yeast extract broth was used as reference samples. Cy-dye labeled cDNA samples were prepared with total RNA from lungs or broth culture, and hybridized on the oligo-microarray slide containing 2,895 genes of L. pneumophila serogroup 1. Virulence gene expression patterns were analyzed using a MIDAS software from TIGR (www. tigr.org). Results: Among a total of 332 virulence genes examined, 17 genes including sidA, lepB, the genes related to flagella assembly (fliR and fliP), LPS lipid A biosynthesis, and the enhanced entry protein EnhA were up-regulated at all four time points. We further confirmed by quantitative real-time reverse transcription PCR that the expression of fliP gene was highly expressed in lung tissue as well as in bronchoalveolar lavage fluids from the mouse infected with L. pneumophila serogroup 1. Conclusions: Through gene expression analysis of L. pneumophila in a mouse model, several candidate biomarkers for diagnosing Legionnaires’ disease could be identified.

부모-자녀 성격유형의 일치와 불일치에 따른 애착수준 및 의사소통유형과의 관계

권창현,심희선 한국심리유형학회 2003 심리유형과 인간발달 Vol.10 No.-

레지오넬라 폐렴의 진단용 바이오마커의 발굴 : A/J 마우스 감염 모델에서 Legionella pneumophila의 독력 유전자들의 발현양상 분석

김승민,심희선,김희남,심호기,윤영경,김정연,박윤선,박대원,손장욱,김민자 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe form of pneumonia. After L. pneumophila is inhaled through contaminated aerosols, it is phagocytized by alveolar macrophages, multiplies in a specialized phagosome approximately 10 h postinfection, and eventually leads to the death of host cells. Currently available diagnostic tests for Legionella pneumonia have some limitations. This study was conducted to find diagnostic biomarkers for Legionella pneumonia using virulence gene expression profiling in a murine experimental model. Materials and Methods: A/J mice were intranasally inoculated with L. pneumophila serogroup 1, and lungs were harvested 4, 8, 24, and 48 h postinfection. The strain grown in buffered yeast extract broth was used as reference samples. Cy-dye labeled cDNA samples were prepared with total RNA from lungs or broth culture, and hybridized on the oligo-microarray slide containing 2,895 genes of L. pneumophila serogroup 1. Virulence gene expression patterns were analyzed using a MIDAS software from TIGR (www.tigr.org). Results: Among a total of 332 virulence genes examined, 17 genes including sidA, lepB, the genes related to flagella assembly (fliR and fliP), LPS lipid A biosynthesis, and the enhanced entry protein EnhA were up-regulated at all four time points. We further confirmed by quantitative real-time reverse transcription PCR that the expression of fliP gene was highly expressed in lung tissue as well as in bronchoalveolar lavage fluids from the mouse infected with L. pneumophila serogroup 1. Conclusions: Through gene expression analysis of L. pneumophila in a mouse model, several candidate biomarkers for diagnosing Legionnaires’ disease could be identified.

청소년의 심리 유형과 애착과의 관계

권창현,심희선 한국심리유형학회 2003 심리유형과 인간발달 Vol.10 No.-

포스터 발표 전시 순서 : 감염 ; 인플루엔자의 진단을 위한 Multiplex-real time RT-PCR assay의 임상적 유용성 평가 (초)

윤영경,천병철,심희선,김남훈,김정연,박대원,손장욱,이창규,김민자 대한내과학회 2009 대한내과학회지 Vol.77 No.4S