배추(Brassica campestris L. var. pekinensis Makino)의 cDNA library 구축 및 상동성 비교

안주미,권오병,전봉균,이풍연,이정민,권무식 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

Expressed sequence tag(EST) has a good value to discover a new gene or to study its structure. Some 20 ESTs were generated to obtain new genetic resources of chinese cabbage(Brassian campestris L. var. pekinensis Makino). Poly A+ RNAs were isolated from 10-day-old seedlings grown at 25℃ under the day light. cDNA gene bank was constructed using λ ZAP /cDNA synthesis /Giga Pack Gold Packing kit. About a million clones were able to obtain from the library. All the clones examined so far had insert DNAs. Nucleotide compositions of randomly selected clones were determined by the Sanger mthod. The DNA sequences were compared with those deposited in the GenePept and GenBank database to figure out nucleotide homologies. Two ESTs showed significant similarities to the enlisted sequences. They are chloroplast GADPH subunit of Arabidopsis thaliana and carbonic anhydrase of A. thaliana. The full DNA sequences of the two clones are being determined. The cDNA gene bank constructed. in this experiment will being used to isolate more genes induced by the light in the plant.

성남시 보건소 유아구강사업 개발을 위한 구강건강실태에 관한 조사연구

안용순,김미정 한국치위생교육학회 2001 한국치위생학회지 Vol.1 No.2

Dental caries causes the majority of tooth loss among Koreans. Korea is experiencing an increase in dental caries. And it shows a high prevalence of caries in preschool children. which contrasted with the much-improved situation in developed countries. So. it is important to control caries from preschool periods in Korea. Recently. inspections of the teeth of preschool children for evidence of dental disease became a legislative duty in Korea following Oral Health Act(2000). But. standardized oral health programs in preschool children is not yet proposed. Therefore, it is necessary to develop an community based oral health program in preschool children. The purpose of this study was to obtain basic data for the development of community based oral health program in preschool children of Sungnam city. Oral health status in primary dentition were surveyed in 862 2-6 year-old preschool children of Sungnam city by WHO criteria. From the data. df rate. percentage of preschool children with active caries, dft index. dt rate, percentage of preschool children with pit and fissure sealant of each age were calculated. and discussed. The obtained results were as follows : 1. The experience of dental caries in primary dentition showed a tendency to increase quickly between 2 and 3 year old children. So. the oral health program in preschool children should be developed to prevent dental caries and control their oral health under three years of age. 2. In 5 year-old children. the percentage with active caries was 60.8% and dft index was 5.06. The dt rate showed a tendency to decrease as proportion to age. but even though 6 year-old children. the dt rate was 47.7%. Therefore. the annual screening dental examination and oral health education programs at the institution for preschool children should be developed to detect and treat dental caries in primary dentition at early stage. 3. The percentage of preschool children with pit and fissure sealant at six years of age was 10.2%. So. pit and fissure sealant and professional fluoride application programs in Public Health Center should be developed to prevent dental caries. 4. Also. it is necessary to establish oral health goals in preschool children and develop water fluoridation program to improve and preserve oral health of preschool children in Seongnam city. effectively.

소양호에서 세균군집 크기에 미치는 폴랑크톤의 영향

안태석,정미정,이은주,조규송 江原大學校 附設 環境硏究所 1996 環境硏究 Vol.13 No.-

To scrutinize the biological effects of plankton communities to bacterial community, the total bacterial numbers, chlorophyll a concentration and phytoplankton, zooplankton communities were analyzed in Lake Soyang from May 13, to July 14, 1994. At 0m depth, total bacterial numbers positively coi-related with chlorophyll o concentration. On the other hand, at 2m depth, the two parameters had negative correlation. At the middle of the June, whe dominants of phytoplankton, zooplankton were changing, the bacterial numbers dropped down. These results suggest that as the Anabaena becoming dominant species, the some zooplankton species changed to bacterivore. The bacterial community was mainly effected by phytoplankton at 0m, and by zooplankton at 2m depth.

성남시 보건소 유아구강보건사업 개발을 위한 구강건강실태에 관한 조사연구

안용순,김미정 한국치위생교육학회 2001 한국치위생학회지 Vol.1 No.2

Dental caries causes the majority of tooth loss among Koreans. Korea is experiencing an increase in dental caries. And it shows a high prevalence of caries in preschool children. which contrasted with the much-improved situation in developed countries. So, it is important to control caries from preschool periods in Korea. Recently, inspections of the teeth of preschool children for evidence of dental disease became a legislative duty in Korea following Oral Health Act(2000). But, standardized oral health programs in preschool children is not yet proposed. Therefore, it is necessary to develop an community based oral health program in preschool children. The purpose of this study was to obtain basic data for the development of community based oral health program in preschool children of Sungnam city. Oral health status in primary dentition were surveyed in 862 2-6 year-old preschool children of Sungnam city by WHO criteria. From the data, df rate, percentage of preschool children with active caries, dft index, dt rate, percentage of preschool children with pit and fissure sealant of each age were calculated, and discussed. The obtained results were as follows : 1. The experience of dental caries in primary dentition showed a tendency to increase quickly between 2 and 3 year old children. So. the oral health program in preschool children should be developed to prevent dental caries and control their oral health under three years of age. 2. In 5 year-old children, the percentage with active caries was 60.8% and dft index was 5.06. The dt rate showed a tendency to decrease as proportion to age, but even though 6 year-old children, the dt rate was 47.7%. Therefore, the annual screening dental examination and oral health education programs at the institution for preschool children should be developed to detect and treat dental caries in primary dentition at early stage. 3. The percentage of preschool children with pit and fissure sealant at six years of age was 10.2 %. So. pit and fissure sealant and professional fluoride application programs in Public Health Center should be developed to prevent dental caries. 4. Also. it is necessary to establish oral health goals in preschool children and develop water fluoridation program to improve and preserve oral health of preschool children in Seongnam city. effectively.

麻黃의 사람 비점막 섬유아세포 monocyte chemotactic protein 중 MCP - 1 , MCP - 2 , 및 MCP - 3 분비에 대한 효과

김현미,이향숙,김정선,조정제,유영천,안현종,최훈,임강현 대한본초학회 2002 大韓本草學會誌 Vol.17 No.1

Recent reports have proposed that Ephedrae Herba may modulate the immune response on allergy or asthma. Human nasal mucosal fibroblasts are a rich source cytokines, inflammatory mediators, and chemokines. Chemokines are important for the recruitment of leukocytes to sites of infection, which is essential in host defense. Objectives : The objective of this study was to investigate the effect of Ephedrae Herba on the release of the chemokines such as monocyte chemotactic protein(MCP)-1, MCP-2, and MCP-3 in human nasal mucosal fibroblasts after stimulation with cytokines like tumor necrosis factor -α(TNF-α), interferon-γ(IFN-γ), and interleukin -1β(IL-1β).

시설농업지에서의 해충과 천적의 발생상

서미자,안수정,박덕기,윤영남 충남대학교 농업과학연구소 1997 농업과학연구 Vol.24 No.1

For the investigation of occurrence of insect pests and their natural enemies, total number of 353 controlled agricultural farms were visited and surveyed. In the 73% of total controlled agricultural farms, 52 species insect pests were occurred. Among these pests, the western flower thrip (Frankliniella occidentalis), the two spotted spider mite (Tetranychus urticae), the greenhouse whitefly (Trialeurodes vaporariorum), the cotton aphid (Aphis gossypii) and the green peach aphid (Myzus persicae) were mainly occurred. On the other hand, small numbers of natural enemies were sustained in 40 controlled agricultural farms, which was 11% and 17% of visited farms and pest occurred farms, respectively. For the most part of collected natural enemies, there were included in Braconid, Coccinellid and Anthocorid, as 63, 20 and 8% of them, respectively.

대전 도덕봉과 백운봉의 산림군락 분류

김효정,이미정,지윤의,안승만,이규석,송호경 한국환경생물학회 2002 환경생물 : 환경생물학회지 Vol.20 No.3

본 연구는 계룡산국립공원의 지류인 도덕봉과 백운봉의 산림식생을 대상으로 식물사회학적 방법에 의해 산림군락을 분류하고 이 지역의 식생 현황 파악과 국립공원관리에 필요한 기초자료를 제공하고자 하였다. 식물사회학적 방법으로 89개 조사구를 분석한 결과, 신갈나무군락, 졸 참나무군락, 굴 참나무군락, 소나무군락, 서어나무군락, 리기다소나무-곰솔군락으로 구분되었다. 신갈나무군락은 해발고가 가장 높고 경사가 급한 지역에 분포하였으며,졸참나무군락은 해발고가 비교적 낮고 경사가 급한 지역에 분포하였다. 그리고 굴참나무군락은 신갈나무군락에 비해 해발고가 낮은 남사면의 건조한 지역에 분포하고 있었다. 소나무군락은 해발고가 비교적 높고 폭이 좁은 건조한 능선 부위에 분포하고 있으며, 서어나무군락은 주로 남사면 계곡부의 중부에 분포하고 있었 다. 그리고 리기다-곰솔군락은 조림군락이다. This study was carried out to classify forest vegetation community of Dodeokbong and Baekwunbong in Gyeryongsan. Employing the releve method of Dierssen, 89 plots were sampled. Forest community was classified into Quercus mongolica community, Quercus serrata community, Quercus variabilis community, Pinus densiflora community, Carpinus laxiflora community, and Pinus rigida-Pinus thunbergii community. Quercus mongolica community was found in high altitude and steep area, Quercus serrata community in relatively low altitude and steep slope area, and Quercus variabilis in southern arid and low area compared with Quercus mongolica community. Pinus densiflora community is present in relatively high altitude and narrow arid ridge area. And, Carpinus laxiflora community is mainly distributed in southern valley area. Pinus rigida-Pinus thunbergii community is planted community.

말티즈개의 구강에서 발생한 골육종

정윤경, 조현예, 이상섭, 김미희, 이계리, 변현섭, 장동우, 안병우 충북대학교 동물의학연구소 2007 Journal of Biomedical and Translational Research Vol.8 No.1

본문참고

A New Sweetpotato Variety for Food and Starch, "Healthymi"

Young Sup Ahn,Byeong Choon Jeong,Mi Nam Chung,Joon Seol Lee,Hag Sin Kim 한국육종학회 2006 한국육종학회지 Vol.38 No.1

Healthymi is a new sweetpotato variety developed from Mokpo Experiment Station, National Institute of Crop Science(NICS), RDA in 2003, which was for the utilization as food and starch. This variety was selected from the cross between Seonmi and MI9001-12

남자대학생의 흡연태도와 행동에 관한 연구

강경진,김정미,민혜영,안소진,오경숙,은경희,이현,이희선,홍주미,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

There have been so many researches about problems of cigarettes and relation of smoking and heath since 1950's and generally known that smoking is one of the worst factor of increasing disease and mortality. No matter what such many proofs about various problems of smoking have been researched, smoking rate of current male collage students has not been decreased. Besides, most of the researches about cigarette use and its addiction have been focused on the adolescent or adult women smokers or adult male, not male collage student. Therefore, the subject of this study is about smoking attitude and smoking behavior of male collage students by general features. Smoking attitude was analyzed by modifying 'Guidelines for the content of tobacco smoking surveys of the general population'(WHO, 1982). Two hundred of male collage students participated to the distributed questionnaire for one week in May. The smoking attitude was categorized into duration of smoking and number of cigarette per day(CPD), and nicotine dependency, investigated by Fagerstrom Tolerance Questionnaire(FTQ) (Fagerstorm & Schneider, 1989). All statistics were performed by procedure of SAS according to number, percentage, mean, standard deviation, and ANOVA. The results are as follows: 1. Smoking attitude level was mean 25.73±5.15 marks, ranging from 13 to 39 marks of the total 40 marks. 2. Duration of cigarettes, number of cigarette per day, and nicotine dependency were mean 5.14±2.84 years, 12.88±6.85 pieces, and 4.78±1.69, respectively. 3. Smoking attitude showed significance according to general features such as marital state and allowance, but the significance according to marital state is not valuable because the numerical difference is so big between married one and single that they cannot be compared with each other. 4. Duration of smoking was significant according to general features such as age and allowance. 5. Smoking amount showed significance the factors of age only. 6. ll1ere was no significance in nicotine dependency according to general features. As a result, smoking attitude of male collage student in this study shows moderate level. Smoking attitude and duration is significant according to allowance, and smoking amount is significant according to age. Nicotine dependency according to general features is not significant but comparing with the preceding study about adult male, male collage students in this study are respectively more nicotine-dependent than adult male smokers. In conclusion, the right allowance usage strategies and effective heath education focused on the young are needed. In addition, helpful programs and information spreading strategies are necessary for establishing higher level of smoking attitude. At last, systemic regulations in daily living for ""anti-nico-dependent"" and perspective reforming of male university student are needed for reducing nicotine dependency.