Effects of plant growth regulator combination on embryo formation for haploid production in wheat

Young-jin Kim,Kyeong-hoon Kim,Hag-sin Kim,Jong-nae Hyun,Jae-hwan Roh,Kee-jong Kim,Kwang-geun Park,Ki-hun Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

Doubled haploid system is a very effective tool which has been widely applied in wheat breeding programmes. Wide-hybridization, wheat X maize cross, is used for the production of wheat doubled haploids (DH). The introduction of doubled haploid (DH) approach into breeding programs has reduced the times and population sizes required for the production of pure lines. We carried out the experiment for development on effective method of producing haploid in wheat. Emasculated spikelets of wheat were pollinated with maize pollen and cultured in the solution containing 40 g/ℓ sucrose and 2,4-D, ABA and GA3 24 h after pollination, and then incubated until embryo rescue. twelve to fourteen days after pollination, the embryos are excised and cultured in half-strength MS basal medium supplemented with 20 g/ℓ sucrose and 1 ㎎/ℓ NAA. The type of plant growth regulators was found to be most significant in production of haploid plants. The application of synthetic auxins to pollinated florets, stimulates haploid embryo development to a stage where the embryos can be rescued onto nutrient media. The percentage of embryos formed was significantly affected by 100 ㎎/ℓ 2,4-D plus 50 ㎎/ℓ BAP and 100 ㎎/ℓ 2,4-D plus 50 ㎎/ℓ GA3. There was varied efficiency in embryo formation from 5.7 to 53%.

Evaluation of wheat type I and II resistance to Fusarium head blight in the greenhouse and field

Sanghyun Shin,Kyeong-Hoon Kim,Hag-Sin Kim,In-Duck Choi,Jong-Chul Park,Jong-Nae Hyun,Kee-Jong Kim,Chul Soo Park,Chon-Sik Kang 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Fusarium head blight (FHB), caused by Fusarium graminearum is a major disease problem on wheat and barley in Korea and around the world. We screened for Type II resistance in the greenhouse using single floret inoculation and for Type I resistance in the field using spray inoculation. Sumai 3 was used the FHB resistant check. Three hundred and seventy lines were evaluated for resistance to spread of symptoms within spike (type II). The 2012 field screening with 300 wheat lines was located in Kimjae-si Joeonbuk Korea. All plots were inoculated twice. The first inoculation was applied at anthesis for wheat. The second inoculation was applied three days after the initial inoculation (dai) for each plot. The inoculum was F. graminearum (GZ3639) prepared at a concentration of 100,000 macroconidai/ml with Tween 20 added as a wetting agent. Mist-irrigation was applied from the first inoculation on May 7 till June 7 to facilitate FHB development. FHB severity was assessed visually 21 days after inoculation on 20 arbitrarily selected spikes per plot. FHB severity was determined as the percentage of symptomatic spikelets from the total of all spikelets observed in these 20 spikes. Based on the field test, we could observe four categories of FHB severity: resistant (R: 0-20%), moderately resistant (MR: 21-40%), moderately susceptible (MS: 41-60%), and susceptible (S: 61-100%). The results showed that forty four lines showed the resistant category on FHB severity between 2.7% and 19.8%. In addition, ten lines showed similar FHB severity compared to Sumai 3 (9.9%).

Influence of whole wheat flour substitution on bread dough fermentation

Induck Choi,Chon-Sik Kang,Kyeong-Hoon Kim,Hag-Sin Kim,Young-Jin Kim,Kyong-Ho Kim,Young-Keun Chenog,Jong-Nae Hyun,Kee-Jong Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Whole grain wheat bread and bakery products is highly considered as a functional food due to the high amount of vitamins, minerals, and dietary fiber in whole wheat flour. However, as the lower end-use properties of whole wheat products limit consumer selection, it is required to measure the functionality of whole wheat flour. Thus, it is observed the fermentation properties of bread dough from the composite flour of whole grain and white wheat flour. Whole grain cv. Chokyeong developed in the NICS was ground using a hammer mill with 0.5 mm sieve. The composite flour was prepared by substituting wheat flour with whole grain flour at 15, 30, 45% levels. Breads were prepared using the 100 g composite flour according to a basic straight-dough mehtod (AACC 10-10A) with slight modification. A rheofermentometer was used to measure the maximum dough height (Hm) and the maximum gas formation (H’m) during fermentation. Also, total CO2 production was recorded. The Hm decreased from 38.8 to 27.5 (mm) with increasing whole wheat flour from 15 to 45%. Whereas, the H’m was increased from 61.7 to 60.8 (mm) with whole wheat flour increment. Total CO2 production was also decreased with increasing whole wheat flour ranging 1312∼1164 (ml). The fermentation results could be useful to partly predict the effect of substituting whole wheat flour on the end-use quality of pan-breads.

Factors on Somatic Embryogenesis and Organogenesis of Soybean (Glycine max)

Kim, Kyong-Ho,Oh, Young-Jin,Suh, Sug-Kee,Kim, Hag-Sin,Kim, Tae-Soo,Lee, Mi-Ja,Park, Ho-Ki,Suh, Duck-Yong,Yeo, Up-Dong 한국콩연구회 2002 韓國콩硏究會誌 Vol.19 No.1

To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as meterial part and size of immature seed, 2,4-D pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon, and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up tp 23%) of somatic embryogenesis was obtained from the immature cotyledon, followed by embryonic axis and immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25%) of embryogenesis was obtained from 3-4 mm length of the seed after 25 days of flowering. The optimum concentration of 2,4-D for embryogenesis was 10mg/l. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tip from germinating somatic embryos, they were cultured on MS medium containing 2 mg/L IBA and 1 mg/L BAP, After then somatic embryos with one and three cotyledons were transferred to the plant growth regulator free medium. The medium exhibited the higher rate (50%) of development than the multiplication medium. To select the section with shoot formation ability, the calli and shoot formation from three sections of 5-days-seedings of soybean were induced on MS medium supplemented with 1.0 mg/L BAP, 3% sucrose, and 0.3% gelrite for one month. The first leaf section exhibited the highest shoot formation rate (51%), followed the hypocotyl section and the cotyledon section. The shoot formation rates and shoot number of the four excised sections of the first leaf were aldo investigated on the same medium. A half of the first leaf explant and the third of the first leaf explant had higher shoot formation rates (76-80%) and number (3-4/ explants) than those in other two explants. Effects of cytokinins on shoot formation were determined, using the half of the first leaf explants. Zeatin (1.0mg/L) exhibited the highest in shoot formation rate (94%) and combination (1: 1, v/v) of zeatin (1.0mg/L) and IAA (1.0 mg/L) eshibited the highest in shoot formation rate (96%) and numbers (16/explant), twice more than zeatin alone (1.0 mg/L). The shoot cuttings were transferred and cultivated on the rooting media supplemented with only auxin, IBA at variois concentrations. The highest root formation (8/shoot) was achieved on the medium supplementes with 1.5 mg/L. After 4 weeks of cultivation, the plantlets with an extensive root system were transplanted in pots with a soil mixture of vermiculite and fine sand. Transferred to field, about 75% of the plantlets survived.

The Factors on Somatic Embryogenesis of Soybean [Glycine max. (L.) Merrill]

Kim, Kyong-Ho,Kim, Hag-Sin,Oh, Young-Jin,Suh, Sug-Kee,Kim, Tae-Soo,Park, Ho-Kee,Park, Moon-Soo,Kim, Seok-Dong,Yeo, Up-Dong The Korean Society of Plant Biotechnology 2000 Plant molecular biology and biotechnology research Vol.2 No.3

To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as material part and size of immature seed, 2,4-D, pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up to 22.9%) of somatic embryogenesis was obtained from the immature cotyledon, following embryonic axis and the immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25.4%) of embryogenesis was obtained from 3-4 mm length of the seed (after 25 days of flowering). The optimum concentration of 2,4-D for embryogenesis was 10 mg/L. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tips from the germinating somatic embryos, they were cultured on MS medium containing 2 mg/L indole-3-butyyic acid (IBA) and 1 mg/L 6-benzyladenine (BA). After then somatic embryos with one and three cotyledons were transferred to the growth regulator free medium. The medium exhibited the higher rate (ca. 50%) of development than the multiplication medium.

Effective Acclimation System for in Vitro Regenerated Plant lets of Soybean

Kim, Young Jin,Park, Tae Il,Kim, Hyun Soon,Suh, Sug Kee,Kim, Hag Sin,Yun, Song Joong The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.2

To establish an efficient acclimation system for regenerated plantlets of soybean, we used various media with hydroponic nutrient solutions before regenerants were transplanted into soil. The hydroponic nutrient solution was essential for the survival of the plantlets. The vermiculite with nutrient solution at pH 5.5 was found to be the best medium with 97-100% survival rate and better growth of regenerants plantlets. Regeneraed grew best in the following order of solutions: Yoshida solution, modified Yoshida solution, SoyI, Soy II, and MS medium. However, Soy I solution (EC 2.9 mS/cm), developed by the Honam Agricultural Research Institute proved to be the most effective for acclimation in terms of the time required for vigorous growth and economical use of chemicals.

Effective Multiplication of Somatic Embryos Using Suspension Culture and Regeneration in Soybean

Kim, Young Jin,Park, Tae Il,Kim, Hyun Soon,Suh, Sug Kee,Kim, Hag Sin,Yun, Song Joong The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.2

The use of liquid-medium-based procedure relative to the solid media led to a 4.5-fold increase in the number of cotyledon-stage embryos. The most efficient system for multiplication and regeneration of somatic embryos was CP6 procedure with the media MSD40/MSD20/MSM6AC/FNL0S3S3GM. However, the rate of regeneration was lower. About 71% of the embryos with dicotyledon were continued to develop the roots after desiccation treatment and 92% of the germinated embryos produced shoots in 10 days. Of the four morphologically different types of embryos, dicotyledonous ones showed a high frequency of conversion, while only a few with fused and horn type cotyledon developed shoots. Mature somatic embryos were desiccated in empty petri dishes for 12-72 h. Embryo survival rate was the highest after 12 h of desiccation, but maximal germination was observed at 24 h. After desiccation, they were placed on MS medium without growth regulators for germination. Germinating embryos were transferred to small pots with vermiculite for plant regeneration. The etiolating the plants during the growth was resolved to add 1% activated charcoal on hormone-free MS medium.

경기장 부대서비스시설 만족이 이벤트이미지와 재관람에 미치는 영향

김학신(Kim Hag Sin) 한국사회체육학회 2003 한국사회체육학회지 Vol.19 No.1

This study was intended to investigate the effect of spectator`s event image and intention to revisit in an attempt to establish the plan for effective operational management of the professional baseball field. As a result, the following conclusion was drawn: First it was found that as for the service facility incidental to the stadium male spectators showed a higher level of satisfaction with factors such as F&B facilities and the bathroom than female counterparts. Seen in terms of age, the group of spectator belonging to the 10s and more than the 40s of age showed the higher level of satisfaction than the other age groups. Seen in terms of occupation, spectators employed in self-employed business, business/specialized technical job, and professional/freelance showed a relatively higher level of satisfaction compared to the other occupational groups. And seen in terms of monthly average income, the club-run concessions and F&B concessions showed the relatively higher level of satisfaction compared to the other income brackets. Especially, it was found that satisfaction with the service facility incidental to the stadium had the significant influence on the event image included the club-run concession, F&B facility, seating, bathroom, parking lots and the like. And it was found that F&B facilities, seating, bathroom and club-run concessions had a significant effect on spectators` intention to revisit the event.

치료받지 않은 고혈압 환자에서 발생한 신동맥류의 자발성 파열

김지일 ( Kim Ji Il ),서학준 ( Seo Hag Jun ),신옥란 ( Sin Og Lan ),송선화 ( Song Seon Hwa ),황은미 ( Hwang Eun Mi ),주강 ( Ju Gang ),김영수 ( Kim Yeong Su ),윤선애 ( Yun Seon Ae ),김영옥 ( Kim Yeong Og ) 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.6

Spontaneous rupture of renal artery aneurysm is a rare, but life threatening complication with high mortality. The etiology of non-traumatic renal artery aneurysm is fibromuscular dysplasia, atherosclerosis, vasculitis, and pregnancy. We here report a case of spontaneous rupture of renal artery aneurysm in a patient with untreated hypertension. A 39-year-old non-pregnant woman complained of sudden onset of right flank and lower quadrant abdominal pain. Ul-trasonography showed large fluid collection in right lower abdomen. Emergency laparotomy demonstrated huge retroperitoneal hematoma due to spontaneous rupture of right renal artery aneurysm. Microscopic examination of the aneurysmal wall revealed intimal hyperplasia without atherosclerotic change. (Korean J Nephrol 2003;22(6):763-766)

양파 육묘 및 정식작업 생력화에 따른 노력 및 비용절감 효과

김학신(Hag Sin Kim),이을태(Eul Tai Lee),장영석(Young Suk Jang),최칠구(Chil Koo Choi),최인후(In Hu Choi),정병춘(Byeong Choon Jeong) 한국원예학회 2003 한국원예학회 학술발표요지 Vol.2003 No.10