전통한의학으로부터 소아성장촉진 바이오소재 개발연구

임강현 충북연구원 2003 충북발전연구원 학술세미나 Vol.- No.-

Porcine Skin Gelatin Hydrolysate Promotes Longitudinal Bone Growth in Adolescent Rats

임강현,이세나,장애라,김혜경 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.5

Collagen hydrolysates (CHs) are mixtures of peptides obtained by partial hydrolysis of gelatin that are receiving scientific attention as potential oral supplements for the restoration of osteoarticular tissues. The aim of this study was to evaluate the effectiveness of CHs for promoting longitudinal bone growth in growing rats. An in vitro study was carried out in osteoblast-like MG63 cells and the most effective CH on bone formation was selected among 36 various CHs. An in vivo study confirmed the functional effects of a selected CH with molecular weight of <3 kDa on longitudinal bone growth. CHs dose-dependently promoted the longitudinal bone growth and height of the growth plate in adolescent male rats, whereas gelatin failed to affect longitudinal bone growth. Insulin-like growth factor-1 and bone morphogenetic protein-2 in the CH treated group were highly expressed in the growth plate. These results suggest that CHs isolated in this study may provide beneficial effects on bone metabolism of growing animals and humans.

다엽(茶葉)의 4 - VO 로 유발한 흰쥐뇌허혈의 신경방어효과

林康鉉 대한본초학회 2001 大韓本草學會誌 Vol.16 No.1

Green tea(茶葉), the leaves of Camellia sinensis (L.) O. Kuntze (Thea sinensis L.), whose property, flavour and channel tropism is bitter and sweet in flavour(苦甘), cool in property(?), acting on the heart, lung, stomach and kidney channels(歸心???經), has the effects of clearing the head and eyes(淸?目), removing the thirst(???), digesting the food(?食), resolving the dampness(化?), facilitating the urination(利?), neutralizing a poison(解?). And this is also one of the most popular beverages in the world. So, this study was planned to check the neuroprotective effect of green tea on the global ischemia induced by 4-vessel occlusion in Wistar rats. Tea extract was lyophilized after extraction with water. We induced 4-vessel occlusion for 10 minutes and reperfused again. The number of CA1 pyramidal neurons were counted after 7 days of reperfusion under the cresyl violet staining. The results obtained that in 4-VO ischemia, Green tea showed significant neuroprotective effects(20 and 100 ㎎/㎏ of green tea extracts, p<0.05 and p<0.001 respectively) compared with control group. Each neuroprotective ratio was about 53.8%, 97.6% respectively. We estimated the amount of the main component (-)epigallocatechin gallate(EGCG) by HPLC, found out its net content in extract was about 9%. However, EGGG given at 10 ㎎/㎏ had HPLC, found out its net content in extract was about 9%. However, EGGG given at 10 ㎎/㎏ had no effect on global ischemia, indicating that EGGG may not be the only main active component of green tea. Immunohistochemical data partially explained the protective effects of green tea via promotion of bcl-2 induction in hippocampus. Consequently, green tea has neuroprotective effects on the global ischemia induced by 4-vessel occlusion in Wistar rats. This work was supported by Korea Research Foundation Grant. (KRF-2000-003-F00311)

Hydrolyzed Collagen Tripeptide Promotes Longitudinal Bone Growth in Childhood Rats via Increases in Insulin-Like Growth Factor-1 and Bone Morphogenetic Proteins

임강현,Sanga Kim,Junsik Lim,박해정,Yong Chul Shin,Joong Su Lee 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.11

Previous studies have reported that collagen tripeptide (CTP) derived from collagen hydrolysate has variousbeneficial effects on health by protecting against skin aging and improving bone formation and cartilage regeneration. Collagen-Tripep20TM (CTP20), which is a low-molecular-weight CTP derived from fish skin, contains a bioactive CTP, Gly-Pro-Hyp >3.2% with a tripeptide content >20%. Herein, we investigated the osteogenic effects and mechanisms of CTP20(<500 Da) on MG-63 osteoblast-like cells and SW1353 chondrocytes. And we measured promoting ratio of the longitudinalbone growth in childhood rats. First, CTP20 at 100 lg/mL elevated the proliferation (15.0% and 28.2%), alkaline phosphataseactivity (29.3% and 32.0%), collagen synthesis (1.25- and 1.14-fold), and calcium deposition (1.18- and 1.15-fold) in MG-63cells and SW1353, respectively. In addition, we found that CTP20 could promote the longitudinal growth and height of thegrowth plate of the tibia in childhood rats. CTP20 enhanced the protein expression of insulin-like growth factor-1 (IGF-1) inMG-63 and SW1353 cells, and in the growth plate of childhood rats, along with Janus Kinase 2, and signal transducer andactivator of transcription 5 activation in MG-63 and SW1353 cells. CTP20 also elevated the expression levels of bonemorphogenetic proteins (BMPs) in MG-63 and SW1353 cells and in the growth plates of childhood rats. These results indicatethat CTP20 may promote the endochondral ossification and longitudinal bone growth, through enhancing of IGF-1 and BMPs. (Clinical Trial Registration number: smecae 19-09-01).

Effects of Albizziae Cortex Extracts on the Elastase Activity and DPPH and NO Scavenging Activities

임강현 한의병리학회 2011 동의생리병리학회지 Vol.25 No.2

Elastic fibers are found in the skin, lungs, arteries, veins and other structures. Elastases destroy the elastic fibers and cause the emphysema and pulmonary hypertension. Oxidative stress is needed for these pathologic changes. Accordingly, present study was designed to investigate the effect of Albizziae Cortex extracts (ACE) on elastase activity and anti-oxidative effects of ACE. The in vitro inhibitory effects on elastase and di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) and nitric oxide (NO) free radical scavenging activities of ACE were measured. The elastase activity was significantly inhibited by ACE. DPPH and NO free radicals were significantly scavenged as well. ACE showed the elastase-inhibiting effects and anti-oxidative activities in vitro. These results suggest that ACE may have potential roles in the treatment of pulmonary emphysema and pulmonary hypertension

Effects of Meretrix Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity

임강현 한의병리학회 2011 동의생리병리학회지 Vol.25 No.3

This study was designed to investigate the collagen metabolism and tyrosinase activity of Meretrix extracts (ME). The effect of ME on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of ME was measured as well. Type I procollagen production was recovered by ME in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by ME. The tyrosinase activity and L-DOPA oxidation were significantly reduced as well. However, the effects on tyrosinase activity and L-DOPA oxidation were not powerful enough to be used as whitening agents. ME showed the anti-wrinkle effects and some whitening effects in vitro. These results suggest that ME may be a useful drug as an anti-wrinkle treatments.

Effect of Cibotii Rhizoma on the Growth Hormone Secretion of Adolescent Male Rats

임강현,부영민,박선영,이동환,김호철 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Effects of Subfractions of Cibotii Rhizoma on Longitudinal Bone Growth of Adolescent Rats

임강현,김명규,김호현,박선영,이동환,부영민,김호철 대한본초학회 2003 大韓本草學會誌 Vol.18 No.3

석고의 생쥐 비장세포 인터루킨-4 분비에 미치는 효과

임강현,김성우,김현미,김정선,조정제,유영천,안현종 한약응용학회 2002 한약응용학회 학술대회 Vol.2002 No.-

구척이 흰쥐의 장골 길이성장에 미치는 영향

임강현,김호철 대한본초학회 2001 大韓本草學會誌 Vol.16 No.2

In this study, we searched some herbal medicines on promoting the heights of longitudinal bone and found that the root of Cibotium barometz promotes longitudinal bone growth. The longitudinal bone growth was measured in the proximal tibia by fluorescence microscope with tetracycline. Tetracycline plays a chelate with calcium ion which is accumulated in bone forming-zones. The accumulated tetracycline made fluorescent lines corresponding to two injections. The growth rate of bones are evaluated by measuring the length between two lines. The longitudinal bone growth rate of control group for two days was 489.0±70.1 ㎛ but, C. barometz administration significantly promoted the longitudinal bone growth(100 ㎎/㎏, 764.2±41.1 ㎛, p<0.01). And the heights of proliferative and hypertrophic zones were also increased in C. barometz administered group measured by Nissl staining. To evaluate its growth promoting mechanisms, the induction of local insulin-like growth factor-1(IGF-1) and bone morphogenetic protein-2(BMP-2) were measured by using immunohistochemical method. C. barometz increased local induction of BMP-2 in hypertrophic and ossification zones, whereas IGF-1 did not increase. These results showed that administration of the extracts of C. barometz promoted longitudinal bone growth and its mechanism could be explained in part by regulation of BMP-2 induction in growth plates.