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Human Aortic Smooth Muscle Cell에서 하엽(荷葉)의 항동맥경화 활성 연구
김선모 ( Sun Mo Kim ),윤현정 ( Hyun Jeong Yun ),이효승 ( Hyo Seung Yi ),원찬욱 ( Chan Wook Won ),김재은 ( Jai Eun Kim ),박선동 ( Sun Dong Park ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: The proliferation and migration of human aortic smooth muscle cells (HASMC) in response to activation by various stimuli plays a critical role in the initiation and development of atherosclerosis. This study was conducted to examine the effects of Nelumbo nucifera leaves (NNL) on the proliferation and migration of HASMC. Additionally, the mechanisms involved in any observed effects were also evaluated. Methods: Apoptotic cells were measured by staining with FITC-labeled annexin V, followed by flow cytometric analysis. The expression level of apoptosis related proteins was confirmed by western blot. And MMP-9 activity was measured by gelatin zymography and MMP-9 expression was measured by ELISA Results: NNL completely inhibited the proliferation of HASMC via induction of the expression of apoptotic proteins including annexin V, cleaved poly ADP-ribose polymerase (PARP), and caspase-3 and -8. NNL treatment resulted in the release of cytochrome c into cytosol, a loss of mitochondrial membrane potential, a decrease in Bcl-2 and Bcl-xL and an increase in Bax expression. NNL also blocked HASMC migration via suppression of MMP-9. Conclusions: Taken together, these results indicate that NNL has the potential for use as an anti-artherosclerosis agent.
부자(附子) 및 천오(川烏)를 포함한 처방이 혈중 Aspartate Aminotransferase, Alanine Aminotransferase 수치에 미치는 영향
한현영 ( Hyun Young Han ),황원덕 ( Won Duk Hwang ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: Aconiti Lateralis Preparata Radix (附子) and Aconiti Tuber (川烏) are not commonly prescribed, but are necessary for some clinical conditions, despite of the fact that some negative effects have been known to occur with these medicines. This study shows the consequences for aspartate aminotransferase (AST), alanine aminotransferase (ALT) due to herbal medicines, including Aconiti Lateralis Preparata Radix (附子) and Aconiti Tuber (川烏). Methods: From 1st June 2007 to 10th May 2009, the results were analyzed for 64 patients belonging to the OO Oriental Medical Hospital who took herbal medicine, including Aconiti Lateralis Preparata Radix (附子) and Aconiti Tuber (川烏), more than 20 days. This is the study about the comparison and the investigation of AST, ALT. And the results were taken upon their hospitalization and to their departure. The Criteria of Diagnosis in Liver Injury of CIOMS was used as a standard for their examinations. Results: AST, ALT (total 64). The number of patients who were within normal reference value: 49. The number of patients who exceed the normal reference value after dosage, without satisfying the Criteria of Diagnosis in Liver Injury: 5. The number of patients who were recovered in normal reference value after dosage: 9. The number of patients who satisfying the Criteria of Diagnosis in Liver Injury after dosage: 1. Conclusions: This results suggest that these herbal medicines, including Aconiti Lateralis Preparata Radix (附子) and Aconiti Tuber (川烏), didn`t cause adverse side-effects on AST, ALT of the patients who are taking them.
RAPD 분석을 통한 대황(大黃)과 종대황(種大黃) 감별용 SCAR 유전자 마커 개발
문병철 ( Byeong Cheol Moon ),이영미 ( Young Mi Lee ),천진미 ( Jin Mi Chun ),이아영 ( A Young Lee ),윤태숙 ( Taesook Yoon ),전명숙 ( Myeong Sook Cheon ),추병길 ( Byung Kil Choo ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: Due to the morphological similarity and frequent occurrence of intermediate forms as well as morphological variations of aerial part, the correct identification between Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma is very difficult. To develop a reliable method for correct identification and improving the quality standards of Rhei Radix et Rhizoma and Rhei Undulatai Rhizoma, we analyzed RAPD and developed SCAR marker. Methods: To amplify target DNA at the genomic level, 32 Operon 10-mer random primers were applied with four Rheum species, R. officinale, R. palmatum, R. tanguticum and R. undulatum. The nucleotide sequences were determined and species-specific primers were prepared depending on the species-specific RAPD amplicons after subcloned into the pGEM-Teasy vector. To develop the SCAR markers, species-specific PCR amplification and mutiplex-PCR were carried out using the single species-specific primer pairs and combinations of them, respectively. Results: We used RAPD analysis of four Rheum plant species to obtain several species-specific RAPD amplicons. From nucleotide sequences of these RAPD amplicons, we developed two SCAR markers that amplified 314 bp and 390 bp DNA fragments in only R. undulatum but not in R. officinale, R. palmatum, R. tanguticum and R. undulatum, for distinguishing Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma. Furthermore, we established SCAR markers for the simultaneous discrimination of the three species within a single reaction by using multiplex-PCR. Conclusions: These genetic markers can be used for the efficient discrimination of plants species and commercial herbal medicines between Rhei Undulatai Rhizoma and Rhei Radix et Rhizoma, to ultimately prevent indiscriminate distribution and prescription of these herbal medicines.
청경사물탕(淸經四物湯) 추출물의 성장기 흰쥐 장골길이 성장에 대한 효과
이정헌 ( Jung Hun Lee ),이세나 ( Se Na Lee ),손재봉 ( Jae Bong Son ),금소현 ( So Hyun Keum ),임강현 ( Kang Hyun Leem ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: This study was designed to investigate the effects of Cheunggyeongsamul-tang extract on the growth of longitudinal bone in adolescent female rats. Methods: Longitudinal bone growth was measured by fluorescent microscopy. To examine the effects on the growth plate metabolism, the total height of growth plate, the induction of local insulin-like growth factor-1 (IGF-1), IGF-1 receptor, bone morphogenetic protein-2 (BMP-2), BMPR-1A, indian hedgehog (IHH), and parathyroid hormone-related protein (PTH-rP) were measured. Results: Cheunggyeongsamul-tang extract enhanced longitudinal bone growth and total height of the growth plate. Also, it promoted the induction of local IGF-1, BMP-2, IHH and PTH-rP of the growth plate. Conclusions: This study shows that the Cheunggyeongsamul-tang extract effects longitudinal bone growth in adolescent rats and might be used for both stunted adolescents and inherent growth failure patients.
TNCB로 유발한 아토피피부염 생쥐 모델에서 천일염가미방(天日鹽加味方)과 청기해독산(淸肌解毒散)의 병용 효과
이경미 ( Kyoung Mee Lee ),김선빈 ( Seon Bin Gim ),최학주 ( Hak Ju Choi ),최정준 ( Jeong June Choi ),노성수 ( Seong Soo Roh ),김동희 ( Dong Hee Kim ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: Atopic dermatitis is a chronic inflammatory disease characterized by typically distributed eczematous skin lesion with pruritus, lichenification and dry skin. In this study, we performed to assess the therapeutic effects of co-treatment of Chenilyeomgamibang (CGB) and Chenggihaedok-san (CHS, C&C) on the TNCB(trinitrochlorobenzene)-induced atopic dermatitis in NC/Nga mice, characterized by the onset of atopic dermatitis along with an increase the number of inflammatory cells and dysregulation of Th2 cytokines. Methods: Defined amount of CGB was sprayed on mice skin and CHS was simultaneously orally administrated to TNCB treated NC/Nga mice for 5 weeks. The immune cell types were caracterized by flow cytometry using each specific antibody. The amount of Th2 cytokines in serum and splenocytes culture supernatant were measured by ELISA. Results: Administration of C&C significantly reduced clinical dermatitis severity including pruritus, edema, eczematous and erythema. Histological findings indicated that the thickening of epidermis/ dermis and dermal infiltration of inflammatory cells were dramatically reduced. Flow cytometry analysis showed that infiltrated immune cell numbers of CCR3+, B220+/IgE+, Gr-1+/CD11b+, and CD117+ were significantly reduced in C&C-treated dorsal skin lesion. Furthermore, T cell composition rate in PBMC was also dramatically decreased by the treatment. C&C greatly down-regulated production of Th2 cytokines including IL-4, IL-5, IL-13 in the serum. The down-regulatory effects of C&C on these Th2 cytokines production were also detected in CD3/ CD28 activated splenocytes. Conclusions: These results indicated that C&C is a plausible therapeutic agent for treatment of atopic dermatitis through regulating the Th2 skewed immune system.
성장관련 한약 혼합제제의 흰쥐 장골 길이성장에 대한 효과
임강현 ( Im Gang Hyeon ),김호현 ( Kim Ho Hyeon ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.3
N/A Objectives : This study was designed to investigate the effects of herbal composition on the growth of longitudinal bone in the adolescent male rats. Methods : Longitudinal bone growth was measured by fluorescence microscopy after injection of tetracycline, which formed fluorescent band to the bone forming sites. To examine the effects on the growth plate metabolism, the heights of growth plate was measured. Results : Treatment of herbal composition (200 mg/kg 및 500 mg/kg) significantly enhanced longitudinal bone growth compared with control group. And herbal composition promoted the height of growth plate. Conclusion : Herbal composition enhanced longitudinal bone growth and promoted the height of growth plate in adolescence male rats .
LDL Receptor Knockout Mouse에서 부추추출물의 죽상경화증 예방 효과
권오준 ( O Jun Kwon ),이주영 ( Jooyoung Lee ),노성수 ( Seong-soo Roh ) 대한본초학회 2016 大韓本草學會誌 Vol.31 No.2
Objectives : The present study was designed to evaluate the protective effect of Allium tuberosum (AT) extract on atherosclerosis in LDL receptor knockout (LDLr KO) mouse fed western diet. Methods : The AT was extracted 70% ethanol. The experimental groups were divided with four groups of LDLr KO mice, one group fed a normal diet and the others fed a Western diets for 8weeks. Two Western diet groups were orally administered AT extract at dosage of 100 and 300 mg/kg body weight. The body weight and food intake were measured every day. We measured levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and Glucose in serum. Also, effect of AT extract performed using H&E staining. Results : The AT treatment groups showed decrease in body weight and food efficiency in comparison with control group. Blood biochemistry parameters such as TG, TC, LDL, and glucose levels were increased in control group, while AT treatment groups were reduced. Also, the increased levels of ALT and AST were improved by AT extract. We confirmed that the weights of liver, kidney, subcutaneous fat, epididymal fat, kidney leaf fat, and intraabdominal fat were change in LDLr KO mice treated AT extract. In addition, histopathological changes in liver and aorta were similar to normal group. Conclusions : Based on these results, the AT extract is considered to make prevention of atherosclerosis through reduction and functional improvement of the liver and vascular endothelial cells in the body fat accumulation and lipid content in LDLr ko mouse model.