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장효원,이미영,오계헌 순천향대학교 기초과학연구소 2001 순천향자연과학연구 논문집 Vol.7 No.2
The purpose of this work was to investigate the microbiological distribution and contamination from korean currency that is circulated generally among various kinds of people. Fifty 1,000-won bills were collected by seasons and regions for 18 months. supernatants derived from bills treated by ultrasonicator with .0.8% saline were inoculated and incubated on various selective media such as mannitol salt agar, potato dextrose agar, plate count agar, bismuth sulfite glucose glycine yeast extract agar, luria-Bertani agar. As the results, various kinds of bacteria and fungi including Staphylococcus aureus, Staphyolcoccus epidermidis, Streptococcus mitis, Streptococcus salivarius, Escherichia coli, Enterobacter sp., Salmonella sp., Pseudomonas sp., Candidasp. from 1,000-won bills grew on the solid media. Bacterial contamination was higher in spring and summer compared to winter, whereas fungal contaminatioj was predominated in winter. Especially, 1,000-won bills collected in fish markets and korean traditional markets were heavily contaminated by E. coli, staphylococcus aureus, Pseudomonas sp. Streptococcus sp.,Salmonella sp., and -hemolytic Bacillus. Similar results were obtained from 5,000-won bills and 10,000-won bills as well. In consequence, it was proven that korean currency of bank notes in current use was significantly contaminated by many pathogenic microorganisms.
절수에 의한 Mongolian Gerbil 장기중량변화에 관한 분석연구
김무강,이기훈,이강이,송치원,이경열,권효정,박미선,정승혁,이행연,김명철 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-
In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.
백합가루 첨가량에 따른 백합병(百合餠)의 관능적·텍스쳐 측정
이효지,정낙원,신수진 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.5
The purpose of this study was to determine gain the most desirable mixture ratio for the ingredients of Backhapbyung. Sensory examination and mechanical test were conducted on the texture, water contents and color degree of Backhapbyung with various ratios of liliibulbs powder and sugar added to nonglutinous rice flour. The results of sensory evaluation showed that Backhapbyung composed of put 8% (24g) liliibulbs powder, 40g sugar, 40ml water and 276g nonglutinous rice flour had the highest overall score in terns of acceptability, after-swallowing and taste of flavor, and overall quality preference. Overall among the recipes tested, that with 6% (24g) liliibulbs powder, 40g sugar, 40m1 water and 282g nonglutinous rice flour showed the highest marks in springiness, cohesiveness, gumminess and chewiness. The moisture content was 33.54% nonglutinous rice flour, and 11.8% liliibulbs powder. The overall quality of sensory examination for bitterness was positively correlated with the hardness of sensory examination and the mechanical examination for adhesiveness. The overall quality of sensory examination for chewiness was positively correlated with the moistness of sensory examination and the mechanical examination for cohesiveness. From the above results, the best mixture ratio of Backhapbyung, with a moisture content of 37.18%, is 8% (24g) of liliibulbs powder, -10g sugar, 40ml water, and 3g salt and 276g nonglutinous rice flour.
윤곽선 정보와 watershed segmentation을 이용한 영역 추출
이원효,설경호,염동훈,고기영,김두영 동아대학교 정보기술연구소 2003 情報技術硏究所論文誌 Vol.11 No.1
This paper proposed a extracting method of the region for image using segmentation and edge information. First proposed algorithm extract information using canny edge detector and the image was divided by watershed segmentation. And it extract the image with edge information by merging region. Finally we compare the proposed method with levelset method. In the result proposed method not only extract the image with accurate region but also reduce operation time.
Lidocaine의 산성도가 경막외 마취에 미치는 영향
이원형,신효철,신용섭 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1
The affective factors on regional anesthesia are lipophilic-hydrophilic balance of local anesthetics, protein binding, hydrogen concentration, structure of intermediate chain, and etc. Local anesthetics in solution exit in a chemical equilibrium between the basic uncharged form(B) and the charged cationic form(BH^+) by Henderson-Hasselbalch equation(PH_a=PK+log[B]/(BH^+)). The increasement of uncharged form brig about rapid onset, good quality, and long duration of anesthesia. Eight patients in group Ⅰ were given 2% lidocaine 21.7ml with 1: 200,000 epinephrine(pH 5.1), and eight patients in group Ⅱ were given 2% lidocaine 20ml with 1: 200,000 epinephrine added NaHCO_3 1.7ml(pH 7.2) to determine the epidural analgesic effect of the pH-adjusted solution. The results are summerized as follow 1. The onsets of analgesia and surgical anesthesia were significantly rapid in group Ⅱ. 2. The levels of analgesia were raised more rapidly in group.
들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향
이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.