鷄胎器官性長에 미치는 Mitoycin C의 影響에 關한 硏究

金武剛,金弘善,崔大卿,趙聲煥,金源式 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

Administration of Mitomycin C into various species of animals has widely been reported since last decade, however, none of them were utilized a growth formula proposed by Zimmerman and Huxley. In this paper we attempt to analyze the specific growth rate in general and organ relative growth rate using the chick embryo under the influence of Mitomycin C which is well known as an antibacterial, antiviral and antitumor by the mechanism of the alkylation. Congenital malformation of chick embryo were not considered. On the sixth day incubation, 8㎍ and 16㎍ of Mitomycin C were injected into the chorioallantoic and the egg was broken for weighing the body weight and individual organs such as brain, heart and lung on the day of 12th, 14th, 16th and 18th. The effects of the growth were analyzed by the analysis of variance method. The parameters included in growth quantity were obtained by the parameters included in growth quantity were obtained by the mathod of the last square using the measured weight, the formulas applied to analysis growth quantity and relative growth were : y=a+bt+ct^2, y=bx^2, and the results this obtained were summarized as follows : When injected with Mitomycin C, the chick embryos were observed with remarkable growth inhibition in all groups of the body, brain, lung and 14th groups of heart (p<0.005) and 12th, 16th, 18th groups of heart(p<0.05). Growth rate was increased with the development in the 8㎍ injected groups of the body, lung and 16㎍ injected groups of the lung, on the other hand, it was decreased in the both injected group of the brain and heart. The specific growth rate of all groups showed tendency of decrease in 8㎍ and 16㎍ Injected groups. The coefficient of relative growth of injected groups to control groups in the various organs showed positive allometry. The growth center of the growth gradient of all injected groups to control groups were on the eighteenth day.

成長에 따른 肝細胞의 量的 變化에 關한 硏究

金武剛,金源式 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

To evaluate the morphological and functional changes of the hepatic cells of hybrid chick according to its growing stages, the volume of cytoplasm, nucleus and nucleolus were measured in 10 specimens of each group; the group of 2nd day, 14th day, 2nd month, 5th month and the group of 11th month after hatching. The results were as follows; 1. The volume of nucleus and nucleolus was increased from the 2nd day to the 14th day after hatching (actively growing period) and from the 5th month to the 11th month after hatching (the period of hypertrophy). The volume chage of cytoplasm appears oppositely to that of nucleus and nucleolus. 2. The nucleoplasmic index was increased at actively growing period and the period of hypertrophy, and the ratio of nucleolar volume to nuclear volume was increased at actively growing period, but unchanged at the period of hypertrophy.

A Histologic Demonstration of Siliceous Materials in Simian Lung Mite Infected Lung Tissues by Microincineration

김무강,James C.S.Kim 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Histologic Demonstration of Siliceous Materials in Simian Lung Mite Infected Lung Tissues by MicroincinerationJames C. S. Kim and Moo-kang Kim1,*President, Diagnostic Forum, New Jersey, USA1College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yusong-Gu, Daejeon 305-764, KoreaReceived April 13, 2003 / Accepted July 18, 2003J. Vet. Sci. (2003), 4(2), 117-123JOURNAL OFVeterinaryScience*Corresponding author: Moo-kang Kim College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea Tel: +82-42-821-6752, Fax: +82-42-825-6752 E-mail: mgkm@cnu.ac.kr

Expression of tyrosine kinase A in the cerebral cortex of postnatal developing rat

김무강,Hyo-Jung Kwon,Kyoung-Youl Lee,Il-Kwon Park,Mi-Sun Park,Mi-Young Lee 대한수의학회 2005 Journal of Veterinary Science Vol.6 No.3

Tyrosine kinase A (TrkA) is an essential component of the high affinity nerve growth factor (NGF) receptor necessary to the mediate the biological effects of the neurotrophins, NGF. This study examined the distribution of TrkA-immunoreactivity (IR) cells in the postnatal rat cerebral cortex and the changes that occur in postnatal development as a result of the expression of this protein. TrkA-IR was detected at postnatal day (PD) 3, PD6, PD9 and PD15. Base upon their somatodendritic morphology, the most commonly labeled cell type was the pyramidal neurons. At PD3 and PD6, layer I, II, III and V was immunopositive for TrkA, at PD9, not only at layer I, II, III, and V but also at layer VI. At PD15, the TrkA-positive cells were distributed in all layers. These TrkA-positive cells were not detected at PD0. In contrast, there was significant increase in the percentage of cells exhibiting TrkA-IR with development and the highest level was detected at PD15. These results suggest that the cerebral cortex expresses TrkA strongly during the postnatalperiod. Moreover, the postnatal evelopment-related increase in the expression of TrkA-cells shows that NGF may have a trophic effect on these cerebral cortex neurons from the postnatal period.

Distribution of trkA in cerebral cortex and diencephalon of the mongolian gerbil after birth

김무강,II-kwon Park,Xilin Hou,Kyung-Youl Lee,O-sung Park,Kang-Yi Lee,Min-young Kim,Tae-sun Min,Geun-Jwa Lee,Won-sik Kim 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.4

TrkA is essential components of the high-affinity NGF receptor necessary to mediate biological effects of the neurotrophins NGF. Here we report on the expression of trkA in the cerebral cortex and diencephalon of mongolian gerbils during postnatal development. The expression of trkA was identified by immunohistochemical method. In parietal cortex and piriform cortex, higher levels of trkA-IR (immunoreactivity) were detected at 3 days postnatal (P3) and at P9. Although trkA was not expressed till P3 in the parietal cortex, it was detectable at birth in the piriform cortex. Several regions, such as Layers I, IV & VI, did not show much expression. Layer I showed especially weak labeling. In the hippocampus, thalamus, and hypothalamus, higher levels of trkA-IR were detected at P6 and P12 than earlier days. But trkA was not expressed at birth in the hippocampus, at P3 in the reticular thalamic nucleus, or neonatally in the dorsomedial hypothalamic nucleus. This data shows that expression of trkA is developmentally regulated and suggests that high affinity neurotrophin-receptors mediate a transient response to neurotrophines in the cerebral cortex and diencephalon during mongolian gerbil brain ontogeny.

Rifampin이 鷄胎의 組織分化 및 成長에 미치는 影響에 關한 硏究

金武剛 충남대학교 의과대학 지역사회의학연구소 1974 충남의대잡지 Vol.1 No.1

This is a study of the effect of Rifampin on the chicken embryo, involving an analysis of the growth of body weight, brain, heart, lung, liver, and kidney. By using the growth formula in varying amounts, its effect on specific growth rate, growth power, and the initial velocity of growth was carefully determined. Moreover, by using a relative growth formula we have compared the coefficient of relative growth and the growth gradient of various organs to body weight. Results of this research are as follows: 1) Rifampin is an inhibiting factor to the growth of the chicken embryo. The 2mg injected group less effect than the 4mg injected group. 2) With the passage of time, growth rate was increased in the following: a) body weight of the control group and the 2mg injected group; b) the liver of the control group and 4mg injected group; c)the lung and kidney of all groups. However, the passage of time showed a decrease in the following: a) the body weight and liver of the 4mg injected group; and b) the brain and heart of all groups. 3) In terms of specific growth rate, all groups showed a tendency toward steady decrease, forming a concave curve line downward. The one exception was the lung of the control group which increased until the fifteenth day. 4) As for the coefficient of relative growth of the various organs to body weight. the kidneys of the control group, the liver and kidneys of the 2mg inected group, and the brain, heart, and lungs of the 4mg injected group showed positive allometry. However, the remainder showed negative allometry. 5) A study of the growth gradient reveals that the liver of the control group and the 2mg injected group, and kidney of the 2mg injected group and the 4mg injected group have a growth center on the 12th day, while the heart of the 2mg injected group has its growth center on the 13th day. The brain of the 2mg injected group and the heart of the 4mg injected group have their growth center on the 14th day; the brain and heart of the control group have theirs on the 15th day. For others the growth center occurs on the 18th day.

Immunohistochemical Localization of Nerve Growth Factor, Glial Fibrillary AcidicProtein and Ciliary Neurotrophic Factor in Mesencephalon, Rhombencephalon,and Spinal Cord of Developing Mongolian Gerbil

김무강,Mi-SunPark,Mi-YoungLee,Keun-JwaLee,Young-GilJeong,Chul-HoLee,Kwon-SooHa,Man-HeeRhee,Kang-YiLee,Kyoug-YoulLee,Chi-WonSong,권효정 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.3

The distribution of the nerve growth factor (NGF),the glial fibrillary acidic protein (GFAP) and theciliary neurotrohic factor (CNTF) was performed incoronal sections of the mesencephalon, rhombencephalonand spinal cord in the developing Mongolian gerbils.Generaly, NGF specificaly recognizes neurons withthe NGF receptor, whereas GFAP does the glia, andwas examined separately in gerbils between embryonicdays 15 (E15) and postnatal weks 3 (PNW 3). TheNGF-IR was first observed in the spinal cord at E21,which might be related to the maturation. The GFAPreactivity was peaked at he postnatal days 2 (PND2),while the highest CNTF-reaction was expresed atPNW 2. The GFAP stains were observed in theaqueduct and the spinal cord, which appeared toproject lateraly at E19. The CNTF was observed onlyafter the birth and found in both the neurons andcerebelum and the spinal cord from PND1 to PNW3.These results uggest hat NGF, GFAP and CNTF areimportant for the development of the neurons and theneuroglia in the central nervous system at he lateprenatal and postnatal stages.

절수에 의한 Mongolian gerbil 시상하부의 Vasopressin 및 Oxytocin 분비세포 변화에관한 면역조직화학적 연구

김무강,이근좌,정영길,송치원,이경열,박일권,이철호,윤원기,손화영 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.1

절수에 의한 Mongolian gerbil 신장조직의 변화에 관한 형태학적 연구

김무강,이근좌,정영길,송치원,이경열,박일권,이철호,이기훈,현병화,김길수,Kim, Moo-Kang,Lee, Keun-Jwa,Jeong, Young-Gil,Song, Chi-Won,Lee, Kyeng-Youl,Park, Il-Koun,Lee, Chul-Ho,Lee, Ki-Houn,Hyun, Byung-Hwa,Kim, Gil-Soo 한국현미경학회 1997 Applied microscopy Vol.27 No.4

Mongolian gerbil (Meriones unguiculatus) has been as an animal model for studing the neurological diseases such as stroke and epilepsy because of the congenital incompleteries in Willis circle, as well as the investigation of water metabolism because of the long time-survival in the condition of water-deprived desert condition, compared with other species animals. In order to accomplish the this research, first of all another divided the laboratory animals 5 groups of which each group include the 5 animals. In this study were investigated the histological structure in the kidney, measured the plasma osmolalities at the time of sacrifice of indivisual animals, and the body weights every day during water-deprived. The results obtained in this study were summarized as followings: 1. The body weights and decreasing rates of the body weight in water-deprived mongolian gerbil groups were continuosly decreased. 2. The plasma osmolalities were increased from the 5th water-deprived day, after then the gradually increasing reached nearly its equilibrium state at the 10th water-deprived day. 3. The urine volumes were abruptly decreased from the 2th water-deprived day, after then the gradually decreasing patterns were reached nearly its equilibrium state at the 10th day, and stopped the 11th day. 4. In the light microscopical observation of the kidney, glomerular capillary loop thickening, mesangial matrix increasing, sclerosis, glomerular cystic atrophy, interstitial fibrosis, tubular dilatation, mononuclear interstitial inflammation, interstitial mineralization, and hyperplasia of the collecting duct epithelium in the cortex area, were observed from the 10th water deprived day, and the lesions were gradually severe changed as the time lapse. 5. In the electron microscopical findings of the kidney, the degenerative changes of endothelial cell, podocyte and mesangial cell in glomeruli were initially observed on the 10th water-deprived day as well as the degeneration of microvilli and intracellular organelle in the renal tubules.

"간장발효,미생물학적고찰"

김무강 중앙대학교 문리과대학 1965 생록원 Vol.8 No.-