Production and characterization of soluble human TNFRI-Fc and human HO-1(HMOX1) transgenic pigs by using the F2A peptide

Park, Sol Ji,Cho, Bumrae,Koo, Ok Jae,Kim, Hwajung,Kang, Jung Taek,Hurh, Sunghoon,Kim, Su Jin,Yeom, Hye Jung,Moon, Joonho,Lee, Eun Mi,Choi, Ji Yei,Hong, Ju Ho,Jang, Goo,Hwang, Joing-Ik,Yang, Jaeseok,Le Springer-Verlag 2014 Transgenic research Vol.23 No.3

The Antioxidant Effect of the Rhaponticum uniflorum Extracts on the Oxidative Stress in H₂O₂ induced Human Keratinocytes

So Hee Ahn,Won Yeoung Choi,Ji Won Jeong,Geon Woo Park,Su Yeon Kang,Eun Jeong Kim,Su Rim Sin,Eun Seo Jang,Gye Won Lee,Young Ho Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, To investigate the antioxidant activities of Rhaponticum uniflorum extracts (RUE), we assessed scavenging activities on DPPH, ABTS radicals, and reducing power. The DPPH and ABTS radical scavenging activities (SC50) of RUE were 96.97±1.19 μg/mL and 31.26±0.13 μ g/mL, respectively, and the reducing power significantly increased dose-dependent manner. To study the antioxidant effecters of RUE in H₂O₂ induced human keratinocytes, expression of Nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot and qRT-PCR. RUE induced the expression of HO-1, a critical target enzyme of Nrf2, in a concentration-dependent manner. The expression of HO- 1 mRNA was also induced in H₂O₂-treated HaCaT cells but no significant difference in the level of HO-1 mRNA was observed. Taken together, these results suggest that RUE may be useful as a potential ingredient for anti-aging products.

Ethanol Extract of Ganoderma lucidum Augments Cellular Anti-oxidant Defense through Activation of Nrf2/HO-1

Lee, Yoo-hwan,Kim, Jung-hee,Song, Choon-ho,Jang, Kyung-jeon,kim, Cheol-hong,Kang, Ji-Sook,Choi, Yung-hyun,Yoon, Hyun-Min KOREAN PHARMACOPUNCTURE INSTITUTE 2016 Journal of pharmacopuncture Vol.19 No.1

Objectives: The mushroom Ganoderma lucidum has been widely used as a traditional herbal medicine for many years. Although several studies have focused on the anti-oxidative activity of this mushroom, the molecular mechanisms underlying its activity have not yet been clearly established. The present study investigated the cytoprotective effect of ethanol extract of Ganoderma lucidum (EGL) against oxidative stress (hydrogen peroxide, $H_2O_2$) and elucidated the underlying mechanisms in a C2C12 myoblast cell line. Methods: Oxidative stress markers were determined by using the comet assay to measure reactive oxygen species (ROS) generation and deoxyribonucleic acid (DNA) damage. Cell viability and Western blotting analyses were employed to evaluate the cellular response to EGL and $H_2O_2$ in C2C12 cells. Transfection with nuclear factor erythroid 2-related factor 2 (Nrf2)-specific small interfering ribonucleic acid (siRNA) was conducted to understand the relationship between Nrf2 expression and $H_2O_2$-induced growth inhibition. Results: The results showed that EGL effectively inhibited $H_2O_2$-induced growth and the generation of ROS. EGL markedly suppressed $H_2O_2$-induced comet-like DNA formation and phosphorylation of histone H2AX at serine 139 ($p-{\gamma}H2AX$), a widely used marker of DNA damage, suggesting that EGL prevented $H_2O_2$-induced DNA damage. Furthermore, the EGL treatment effectively induced the expression of Nrf2, as well as heme oxygenase-1 (HO-1), with parallel phosphorylation and nuclear translocation of Nrf2 in the C2C12 myoblasts. However, zinc protoporphyrin IX, a HO-1 inhibitor, significantly abolished the protective effects of EGL against $H_2O_2$-induced accumulation of ROS and reduced cell growth. Notably, transient transfection with Nrf2-specific siRNA attenuated the cytoprotective effects and HO-1 induction by EGL, indicating that EGL induced the expression of HO-1 in an Nrf2-dependent manner. Conclusion: Collectively, these results demonstrate that EGL augments the cellular anti-oxidant defense capacity through activation of Nrf2/HO-1, thereby protecting C2C12 myoblasts from $H_2O_2$-induced oxidative cytotoxicity.

부산·울산·경남지역 직업병 감시체계

김정일,김병권,김정원,채창호,이철호,강동묵,김지홍,김진하,김영욱,이영하,이지호,김정호,윤형렬,유철인,정백근,장태원,김운규,윤동영,강진욱,김종은,안진홍,이동준,장준호,이광영,송혜란,최영희,이용환,조병만,최홍렬,고상백,김은아,이유진,홍영습,정갑열,김정만,김준연 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.1

Objective: Occupational medicine specialists in the Busan, Ulsan and Kyung-Nam areas established an area-based occupational disease surveillance system and used this system to collected case information for the purpose of preventing occupational diseases Methods: l l hospital participated in this system. The authors selected five main diseases, which were hand-arm vibration syndrome (HAVS), work-related musculoskeletal disorder (WRMSD), occupational asthma, occupational skin disease and occupational and occupational lung cancer and established their case definitions. All cases were reported on the web, and real time analysis was conducted. Results: Between April 2001 and April 2003, 192 cases of HAVS, 118 cases of WRMSD, 33 cases of occupational asthma, 17 cases of occupational skin disease and 17 cases of occupational lung cancer (for a total of 377 cases of these five main diseases) were reported. most of the HAVS cases came from shipbuilding, and 172 of them (92.7%) were associated with grinding. Of the four main types of WRMSD, carpal tunnel syndrome (CTS) was the most prevalent with 46 cases and shipbuilding was also the main industry involved (83.9%). The ergonomic risk factors involved mainly associated with the hand. In 19 (57.6%) and 4 (12.1%) cases of occupational asthma, the agents involved. The causative agents of occupational lung cancer included Cr, welding fume, PAH, Ni, etc. 10(58.8%) of the cases were diagnosed as squamous cell carcinoma and 5(29.4%) as adenoma. Conclusion: This result showed that an area-based occupational disease surveillance system might provide an effective method of evaluating the prevalence of such diseases, however the Busan, Ulsan and Kyung-Nam provinces are too large to be treated as individual areas. Therefore, the authors suggest that each province should establish its own surveillance system.

AB 슬라이드, 크런치 및 윗몸일으키기 운동이 복직근 근활성도에 미치는 효과 : Comparison of It `s Myoelectrical Activities with Crunch and Sit-Up

지송운,김호성,양윤준,장재원 대한스포츠의학회 2004 대한스포츠의학회지 Vol.22 No.1

The purpose of this study was to compare the effect of AB slide™ on abdominal muscles with Crunch and Sit-up. Myoelectrical activities of Rectus abdominis muscles produced by AB slide™, Crunch and Sit-up were recorded. Healthy male aged from 17 to 36 years(23.0±5.9 yrs) were included fo the test. Surface electrodes were attached to upper and lower part of Rectus abdominis of the study subjects. Averaged integrated electromyography(AIEMG) and Normalized integrated electromyography(NIEMG) were recorded. The results are summarized as follows; First, the AIEMG of Sit-up were highest among them with insignificant difference statistically. Second, the NIEMG of upper part of Rectus abdominis were higher than the one of lower part with significant difference statistically(p<0.05). This phenomenon was same irrespective of the type of exercises. AB slide™ was not more effective than Sit-up and Crunch for strengthening of Rectus abdominis muscle. The upper part of Rectus abdominis was more activated than the lower part by all three exercises.

身體 發達 推移에 關한 硏究 : 國民學校 學生들을 中心으로 centering into the primary school pupils

蔣址奎,丁光福,鄭成浩 진주산업대학교 1981 論文集 Vol.19 No.-

In order to study on the transition of the development of body, a comparison of the result of physical examirations of the 68 primary school pupils under the juridiction of the Jinjoo Education Bureau was made, and from which the following conclusion was obtained. 1. For the comparison of boys and girls by age and the year, the incrdase of each age and year, it was developed gradually, and with a little difference between 1975 and 1976, it was revealt that result for 1975 was high. On the other hand, the increase per year has shown a similar increase per year for the result of all sorts excluding the height. Accordingly for the height, it was revealt as the highest increase per yaer. 2. For the comparison of the result of the physique for boys and girls, theheight, the weight and the sitting height have shown that boys were bigger than girls in each year but for only the of age of 11 that were bigger than boys showing a little difference. Especially for the circumference of the chest, it was revealt that the result of the boys has shown the highest difference. 3. In the comparison of the result of test of physique with the national standards for boys, the result of all other sorts excluding the height and the weight of the age of 9. the pupils under the juridiction of the Jinjoo Education. Bureau was superior to that of girls. And for girls, the result was superior to boys as shown for the boys in exception of little years by sorts.

상추(Lactuca sativa L.)의 ACAT 억제 Diterpenoid, Phytol : 식용 식물자원으로부터 활성물질의 탐색 Development of Biologically Active Compound from Edible Plant Sources

장태오,방면호,송명종,홍윤희,김지영,정대균,배동근,권병목,김영국,이현선,김인호,백남인 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

排球選手의 早期 運動衝擊이 手骨에 미치는 影向

蔣址奎,鄭成浩 진주산업대학교 1982 論文集 Vol.20 No.-

The present study was designed to analyse eariy-stage volley ball player's metacarpal bones aed phalanx histologically, pathologically by X-rays photographing. The main findings of the study were as follows; 1. Main attack plater among early-stage volley hall players, whose game carrier was longer than three years, was disposed to be shorter than assist attack player in epiphtseal line assimilation completion period. 2. Among ten early-stage volley ball player, J. H. Rho, J. J. Su & C. H. Kim almost completed epiphtseal line assimilation of metacarpal bones and phalanx. G. N. Kim & E. J. Kim wholly completed epiphtseal line assimilation with the acceleration phenomenon of metacarpal bones and phalanx resulting from the movement impulse by external power. M. H. O was completing epiphtseal line assimilation, G. H. Kang, W. H. Pack & M. S. Jung showed initial phenomenon. G. O. Wo made a complete failure in epiphtseal line assimilation of metacarpal bones and phalanx. 3. For all that the movement impulse by exteral power confimusously stimulated, early-stage volley ball player showed no pathological phenomenon. 4. Though the movement impulse through excessive training stimulated metacarpal bones and plalanx, early-stage volley ball player's bones and phalanx, carly-stage volley boll player's bones deformity and bones growth form was normal.

Flavobacterium meningosepticum에 의한 균혈증 1예

장성원,유진홍,진승원,김선우,김연식,박순민,이대훈,최민호,최주연,신완식,강문원,강지민 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

1996년 6월 카톨릭대학교 부천성가병원에 뇌경색으로 입원했던 61세 남자 환자의 혈액에서 Flavobacterium meningosepticum을 분리하였다. 환자는 폐렴을 동반한 패혈증의 예로 ceftriaxone과 metronidazole을 투여하였으나 입원 48시간만에 사망하였다. Flabovacterium meningosepticum is a rae cause of nosocomial infection which shows multi-drug resistance. It mainly invades patients with impaired immunity. Recently, we experienced a case of F. meningosepticum bacteremia in a patient with chronic debilitated state owing to stroke. The clinical progress was so rapid that he died within 48 hours. Blood culture revealed F. meningosepticum which showed characteristic yellow colony in blood agar plate.

김대중 정부의 대기업 구조조정정책 연구 : 역사적 제도주의의 적용 Based on Historical Institutionalism

장지호 한국정책학회 2003 韓國政策學會報 Vol.12 No.2

본 논문은 1997년 경제위기 이후의 김대중 정부가 시행하였던 대기업 구조조정정책의 결정과 집행과정에 있어서의 기존 제도의 경로 의존적 제약을 강조한다. 본 논문은 정책에의 제약을 가져오게 된 제도적 경로를 정부주도의 정책사고와 사유화된 대통령의 권력, 그리고 통제된 금융으로 파악한다. 국민정서와 법에 기반을 둔 정부주도의 정책사고는 민간기업의 경영에 대한 정부의 간섭과 행정규제의 기제로 작용한다. 대기업을 상대로 정책을 펼칠 경우 이러한 정책사고는 사유화된 대통령 권력의 사회적 용인과 맞물려 법이 뒤로 밀리고 정부가 주도한 약속이나 압박 등의 방식이 더 큰 위력을 발휘하게 한다. 또한 부실 금융기관을 정리·통폐합하고 재무구조 개선의 목적으로 공적자금을 투입한 결과 정부의 금융권에 대한 통제력은 강호되어 민간금융기관이 정부의 정책실현수단으로 이용된다. 본 연구는 정책구조의 제도적 제약을 살펴봄으로서 정책결정의 메커니즘을 발견한다. 역사적 맥락에 의해 형성된 제도가 경로의존적 특성으로 정책유형을 결정하는 상황 하에서는 효율적으로 여겨지는 정책일지라도 그의 선택이 용이하지 않다. 상호결합 되어있는 제도들의 변화가능성이 상당히 제약적이고 선행되었던 공식적·비공식적 제도의 경로가 정책변화의 범위를 규정하기에 정책을 시행하고 평가함에 있어서 장기적인 기준으로 행해져야 함을 말해주고 있다. Based on historical institutionalism, this study argues that there is continuity in institutions, emphasizing the constraints created by existing structures and institutions. This study assesses the Republic of Korea as a case study analyzing big business policy under the Kim Dae Jung government. The result found in this study is that there is no rapid disappearance of institutional pattern of state activism in Korea. This study examining four institutions : 1) state-led ideology : 2) centralized and personalized power of the president and bureaucratic system as a function of policy implementation : and 3) the state-advised financial sector. This study demonstrates that whereas formal institutions collapse or are dismantled, informal institutions such as operating procedures persists to shape the behavior of political actors. Institutional structure is so integrated that it cannot be easily divided into separate parts for the new regime in order to produce different or 'efficient' solutions to industrial restructuring.