전략적 데이터베이스 마케팅 구축과 활용 : 프로젝트중심의 구축 방법론과 마케팅전략을 중심으로

이병원,최영랑 동양대학교 1997 동양대학교 논문집 Vol.3 No.1

청소년 인터넷 중독에서의 내현적 자기애에 관한 연구

최원희(Won-Hee Choi),손정우(Jung-Woo Son),김영랑(Yeoung-Rang Kim),이상익(Sang-Ick Lee),신철진(Chul-Jin Shin),김시경(Sie-Kyeong Kim),주가원(Ga-Won Ju) 대한소아청소년정신의학회 2011 소아청소년정신의학 Vol.22 No.2

Objectives:This study compared covert narcissistic propensity in adolescents with internet addiction tendency to normal adolescents. Further, we investigated the correlation between such propensities and anonymity in cyberspace, presence feeling and interactivity in internet gaming, and achievement motivation in adolescents with internet addiction tendencies. Methods:Male middle school students with internet addiction tendencies (Addiction Tendency Group, N=27) and normal students (Control Group, N=29) were recruited. The scale of internet use, Covert Narcissism Scale, scale of achievement motivation, scale of anonymity in cyberspace, scale of presence feeling in internet games, and the scale of interactivity in internet games were administered. A comparison of the average scores and correlation analyses were performed. Results:1) Compared with Control Group, the Addiction Tendency Group showed significantly higher scores on all subscales of the Covert Narcissism Scale, and both the scale of presence feeling and that of interactivity in internet games. Further, the score on the scale of achievement motivation for the Addiction Tendency Group was significantly lower than that of the Control Group. 2) In the Addiction Tendency Group, the scores on several subscales of the Covert Narcissism Scale were significantly positively correlated with the scores on the scale of presence feeling in internet games, anonymity in cyberspace, and interactivity in internet games. However, in the Control Group, the scores of several subscales on the Covert Narcissism Scale were significantly negatively correlated with the score on the scale of achievement motivation, and was significantly positively correlated with the score on the scale of interactivity in internet games. There were no other significant correlations between the scores on the subscales of the Covert Narcissism Scale and the scores of either presence feeling in internet games or anonymity in cyberspace. Conclusion:These results suggested that adolescents with a tendency to be addicted to the internet exhibited higher covert narcissistic propensities than normal adolescents and that the covert narcissistic tendencies were related with presence feeling in cyber games as well as with anonymity in cyberspace.

각질 형성 세포에서 TNF-ɑ로 유도된 염증 반응에 대한 흑삼 추출물의 항염증 효과

최원영(Won Yeoung Choi),이계원(Gye Won Lee),권득상(Deuk Sang Kwon),신현중(Hyun Joong Shin),조영호(Young Ho Cho) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.10

본 연구에서는 85°C에서 9번 증숙한 흑삼 추출물의 총 폴리페놀 함량 및 항산화 활성을 측정하고, TNF-α 유도 피부 각질 형성 세포에서 항염증 효과와 LPS로 유도된 대식세포에서 NO 생성 억제능을 확인하였다. 그 결과 총 폴리페놀 함량은 증숙 전에 비해 증숙 후에 약 2.5배 증가하는 것으로 나타났다. 흑삼 추출물의 항산화 활성을 DPPH, ABTS, superoxide anion 라디칼 소거 활성 및 환원력으로 평가한 결과 증숙 후 흑삼 추출물이 증숙 전에 비해 항산화 활성이 높아지는 것으로 나타났다. Mouse 대식세포인 Raw 264.7 세포에 LPS를 처리한 후 NO 생성량을 확인한 결과 증숙 후 흑삼 추출물의 경우 LPS 단독 처리군에 비해 NO 생성량을 농도 의존적으로 감소시켰다(P<0.001). NO 생성량 감소에 따라 TNF-α로 자극된 피부 각질 형성 세포에서 친염증성 사이토카인인 IL-6, IL-1β 및 IL-8의 mRNA 발현량을 확인한 결과, 흑삼 추출물의 농도 50과 100 μg/mL에서 IL-6의 mRNA 발현을 TNF-α 처리군 대비 약 20% 감소시켰으며 IL-1β와 IL-8의 발현은 농도 의존적으로 감소시키는 것으로 나타났다. 흑삼 추출물이 친염증성 사이토카인의 유전자 발현을 감소시키는 것으로 나타났기 때문에 흑삼 추출물의 항염증 작용을 규명하기 위하여 염증신호전달에 관여하는 전사인자인 NF-κB와 염증 반응을 유도하는 단백질 중 하나인 iNOS와 COX-2 발현에 미치는 영향을 측정하였다. 그 결과 흑삼 추출물 처리 시 TNF-α 처리에 의해 증가된 NF-κB의 인산화와 염증 반응매개물질인 iNOS 및 COX-2의 발현을 유의적으로 감소시키는 것으로 확인되었다(P< 0.001). 이러한 흑삼 추출물의 항염증 효과는 NF-κB의 인산화 억제를 통해 염증 매개 분자의 발현을 감소시켜 친염증성 사이토카인의 생성을 억제하여 나타나는 것으로 확인되었다. 따라서 흑삼 추출물은 피부 염증 개선을 위한 관련 제품의 소재로 활용이 가능할 것으로 기대된다. We evaluated the antioxidant activity and anti-inflammatory effects of black ginseng manufactured by a steaming process in a cell-based inflammatory model. Free radical (DPPH, ABTS, superoxide anion) scavenging activity, reducing power, and total polyphenol content in the extracts of ginseng and black ginseng were investigated. The total polyphenol content of the black ginseng extract was 38.83±1.01 mg/g, which was 2.5 times higher than that of the ginseng extract. Also, the black ginseng extract showed higher free radical scavenging activity than the ginseng extract. The anti-inflammatory effect on lipopolysaccharide (LPS, 50 ng/mL)-induced inflammatory reactions in RAW 264.7 cells demonstrated that treatment with black ginseng extract decreased nitric oxide (NO) and inducible nitric oxide synthase (iNOS) production in a dose-dependent manner (P<0.001). The quantitative RT-PCR result indicated that the black ginseng extract markedly reduced the expression of pro-inflammatory cytokines (interleukin (IL)-6, IL-1β, and IL-8) mRNA in tumor necrosis factor (TNF)-α-treated human keratinocytes. Furthermore, the reduced mRNA expression was associated with the downregulation of iNOS, cyclooxygenase (COX)-2, and nuclear factor (NF)-κB activity. These results suggest that black ginseng extract exhibits anti-inflammatory effects by the downregulation of NF-κB and the consequent suppression of inflammatory mediators (iNOS and COX-2) in TNF-α-induced human keratinocytes and suppression of NO production in LPS-induced murine macrophages. Taken together, these results indicate that black ginseng extract could be a valuable anti-inflammatory agent in products used to treat skin inflammation.

뽕잎 품종별 기능성 성분 분석

채주영(Joo-Yeoung Chae),이준영(Jun-Young Lee),황인승(In-Sung Hoang),황보득(Duk Whangbo),최필환(Pil-Whan Choi),이완주(Won-Chu Lee),김진원(Jin-Won Kim),김선여(Sun-Yeou Kim),최상원(Sang-Won Choi),이순재(Soon-Jae Rhee) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.1

본 연구는 뽕잎을 이용한 고부가가치의 기능성식품의 제조에 적합한 뽕잎 품종을 선별하고자 품종별(YK-209, Cheong-il, Yongchon and Kaeryang), 산지별(영천 및 상주), 부위별 (상위, 중위 및 하위엽) 및 생육시기별(5월, 6월 및 8월)에 따른 뽕잎의 기능성 지표물질인 DNJ, GABA 및 flavonoid의 함량을 HPLC로 정량하였다. 4가지 뽕잎 중 YK-209 뽕잎의 기능성물질(DNJ, GABA 및 flavonoid) 함량이 가장 높았다. 그리고 YK-209 뽕잎으로부터 분리된 4가지 flavonoid 중 rutin 함량이 가장 높았으며, 그 다음으로 isoquercitrin>kaempferol-3-O-rutinoside>astragalin 순으로 나타났으나 그 외 3가지 뽕잎에서는 isoquercitrin의 함량이 가장 높았고 그 다음으로 rutin이 차지하였으나 품종별, 산지별, 부위별 및 성장 시기별에 따라 flavonoid 구성성분의 함량이 다소 차이가 있었다. 영천지역에서 수확한 YK-209 뽕잎의 3가지 기능성물질의 함량이 상주에서 수확한 뽕잎보다 그 함량이 높았다. 한편, YK-209 뽕잎의 부위 중 상위엽이 중위 및 하위엽보다 3가지 기능성물질의 함량이 높았다. 끝으로 YK-209 뽕잎의 성장시기 중 5월에 수확한 것이 6월 및 8월에 수확한 뽕잎보다 기능성물질의 함량이 높았다. 이러한 연구결과를 미루어볼 때 3가지 기능성성분 함량이 높은 YK-209 뽕잎(용천뽕과 개량뽕을 교배하여 얻은 것)의 5월산 상위엽을 향후 뽕잎의 이용한 기능성식품의 제조용 원료로 활용할 수 있을 것으로 기대된다. Quantification of functional components, such as [1-deoxynojirimycin (DNJ), γ-aminobutyric acid (GABA) and flavonoids] of four different cultivars (YK-209, Cheongil, Yongchon and Kaeryang) and parts (upper, middle and lower) of mulberry leaves harvested in different areas and periods was performed by HPLC to select the best quality of mulberry leaf suitable for manufacture of functional foods. Among four mulberry cultivars, YK-209 mulberry leaf had the highest DNJ, GABA and flavonoids levels. YK-209 mulberry leaf harvested in “Yeongcheon” area had greater DNJ, GABA and flavonoid contents than those harvested in “Sangju” area. Four major flavonoids of mulberry leaf were rutin, isoquercitrin, kaempferol-3-O-rutinoside, astragalin, and their levels varied greatly with cultivars. YK-209 mulberry leaf had the highest flavonoids content among four cultivars. DNJ, GABA and flavonoid contents of young YK-209 mulberry leaf were relatively higher in the upper part than those in the middle and lower parts. Additionally, levels of DNJ, GABA and flavonoids were higher in mulberry leaves harvested in the eariler growing season (May) than in the late growing seasons (June and August). Particularly, among four flavonoids, rutin content had the highest in the mulberry leaf harvested in May, while isoquercitrin content did the highest in the mulberry leaf harvested in June and August. These results suggest that YK-209 mulberry leaf having high amount of functional components, such as DNJ, GABA and flavonoids, may be useful as potential source of beverages and tablets.

Estimating environmental fate of tricyclic antidepressants in wastewater treatment plant

Choi, Jong-Won,Zhao, Yufeng,Bediako, John Kwame,Cho, Chul-Woong,Yun, Yeoung-Sang Elsevier 2018 Science of the Total Environment Vol.634 No.-

<P><B>Abstract</B></P> <P>TCAs are known to be toxicants and endocrine disrupting agents. Generally, after being used, TCAs are passed through wastewater treatment plants (WWTPs) to be treated. However, still trace amounts (ng/L to μg/L) of TCAs have been founded even in the treated water. Therefore, the aim of this study is to elucidate the environmental behaviors of TCAs in the sewage water from WWTPs (Jeonju, Korea). For the experiments, seven TCAs (amitriptyline, imipramine, clomipramine, desipramine, protriptyline, nortriptyline, and doxepin) were selected. Hydrolysibility, biodegradability, and adsorbability of the selected seven TCAs were evaluated. Based on the results, it was concluded that TCAs are not readily hydrolyzed in water and also not biodegraded by aerobic sludge. The 60% to 85% of TCAs were adsorbed immediately onto the activated sludge within 1 s via electrostatic and hydrophobic interactions. It was clearly observed that adsorption affinities were dependent on the types of activated sludge (i.e. anaerobic and aerobic sludge). The affinities of aerobic and anaerobic sludge towards the TCAs at trace concentrations e.g., 1 to 10 μg/L, were estimated to be in the range from 0.021 ± 0.000 to 0.087 ± 0.000 L/μg and from 0.001 ± 0.000 to 0.108 ± 0.001 L/μg, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> TCAs were neither readily hydrolyzed nor readily biodegraded under conditions of WWTPs. </LI> <LI> TCAs were quickly adsorbed onto the activated sludge via electrostatic and hydrophobic interactions. </LI> <LI> The adsorption affinities depended on the types of activated sludge, i.e. aerobic and anaerobic. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

1인 가구를 대비를 위한 일본 소형공동주택 계획특징에 관한 연구

최태원(Choi, Tae-Won),김영훈(Kim, Yeoung-Hoon) 대한건축학회 2012 대한건축학회 학술발표대회 논문집 Vol.32 No.2

pCT105로 유도된 치매모델에서 석창포(石菖蒲) 수추출액(水抽出液)이 미치는 영향

최혁 ( Hyuk Choi ),김상호 ( Sang Ho Kim ),이대용 ( Dae Yong Lee ),안대종 ( Dae Joong Ahn ),강형원 ( Won Hyung Kang ),류영수 ( Yeoung Su Lyu ) 대한한방신경정신과학회 2002 동의신경정신과학회지 Vol.13 No.2

Palladium recovery from aqueous solutions using Corynebacterium glutamicum biomass

Sung Wook Won,In-Seob Kwak,Sun Beom Choi,Juan Mao,Sok Kim,M. Sathishkumar,Min A Bae,Yeoung-Sang Yun 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.10

Multistage Operation of Airlift Photobioreactor for Increased Production of Astaxanthin from Haematococcus pluvialis

( Yoon E Choi ),( Yeoung Sang Yun ),( Jong Moon Park ),( Ji Won Yang ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.10

An internally radiating photobioreactor was applied for the production of astaxanthin using the unicellular green alga Haematococcus pluvialis. The cellular morphology of H. pluvialis was significantly affected by the intensity of irradiance of the photobioreactor. Small green cells were widespread under lower light intensity, whereas big reddish cells were predominant under high light intensity. For these reasons, growth reflected by cell number or dry weight varied markedly with light conditions. Even under internal illumination of the photobioreactor, light penetration was significantly decreased as algal cells grew. Therefore, we employed a multistage process by gradually increasing the internal illuminations for astaxanthin production. Our results revealed that a multistage process might be essential to the successful operation of a photobioreactor for astaxnthin production using H. pluvialis.

Adsorptive interaction of cationic pharmaceuticals on activated charcoal: Experimental determination and QSAR modelling

Zhao, Yufeng,Choi, Jong-Won,Bediako, John Kwame,Song, Myung-Hee,Lin, Shuo,Cho, Chul-Woong,Yun, Yeoung-Sang Elsevier 2018 Journal of hazardous materials Vol.360 No.-

<P><B>Abstract</B></P> <P>Due to high mobility and specific toxic actions of the ionizable pharmaceuticals in surface water with a normal range of pH, the pharmaceuticals should be removed before being discharged. Therefore, this study investigated the adsorptive interactions between cationic pharmaceuticals and a popular adsorbent (i.e., activated charcoal) frequently used in water treatment processes. For that, we performed isotherm experiments and then the results were plotted by Langmuir model to determine the adsorption affinity (<I>b</I>) and capacity (<I>q<SUB>m</SUB> </I>). Afterwards, to interpret the adsorption behaviors, two simple prediction models were developed based on quantitative structure-activity relationships (QSAR). In the modelling, molecular weight (MW), polar surface area (PSA), and octanol-water partitioning coefficient (log <I>P</I>) were used as model parameters. In the results, the combinations of these three parameters could predict the adsorption affinity and capacity in <I>R</I> <SUP>2</SUP> of 0.85 and 0.80, respectively. The robustness of models was validated by leave-one-out cross-validation (<I>Q</I> <SUP>2</SUP> <SUB>LOO</SUB>) and the estimated <I>Q</I> <SUP>2</SUP> <SUB>LOO</SUB> values were 0.60 and 0.55 for the adsorption affinity and capacity, respectively, which are higher than the acceptability of standard i.e., 0.5.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Adsorptive interactions between cationic pharmaceuticals and activated charcoal were obtained at natural pH. </LI> <LI> Simple and easy parameters were used to predict adsorptive behavior through QSAR-based modelling. </LI> <LI> Major factors influencing the adsorption include log <I>P</I>, polar surface area, and molecular weight of pharmaceuticals. </LI> <LI> The log <I>P</I> value of pharmaceutical was the most important factor. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>