혈당 측정에 있어서 반사광 비색법과 전기 화학 전극법의 비교

황유성,이도훈,우재근 충북대학교 의과대학 충북대학교 의학연구소 1995 忠北醫大學術誌 Vol.5 No.1

당뇨병환자에서 적절한 혈당을 유지하기 위한 목적으로 시험지봉 검사 방법이 1970년대 초 처음으로 개발된 이후 많은 자가 혈당기들이 고안되어 사용되고 있으며, 대부분이 반사광 비색법을 이용한 자가 혈당 측정기로 현재 국내에는 Glucometer(Ames), Glucoscan(Eiken), One TouchTRM Ⅱ(Lifescan), Accu-check Ⅲ(BM)등이 사용되고 있다. 저자들은 본원에서 이미 사용하고 있었던 반사광 비색법을 이용한 시험지봉으로 LIFESCAN사의 One TouchTM Ⅱ와 전기 화학 전극법을 이용한 Medisense사의 CompanionTM 2에 대하여 정밀도, 직선성, 비교방법과의 상관관계 및 혈액량과 헤마토크리트에 의한 영향, 관찰자간의 변이, 항응고제가 미치는 영향 등 측정에 영향을 미치는 여러 인자들에 대하여 평가를 시행하였다. 검사일내 정밀성(within day precision)은 두 기기 모두 5% 정도이었고, 검사일간 정밀성(day-to-day precision)은 CompanionTM 2가 3.14%∼6.53%, One touchTM Ⅱ가 4.69%∼8.13%의 변이계수를 보였다. 두 기기 모두 직선성이나 비교방법과의 상관관계가 우수하였다. CompanionTM 2와 One touchTM Ⅱ사이의 상관관계는 y=1.040x+21.679, r=0.9716이었다. 두 기기 모두 혈액량, 관찰자, 항응고제에 의해서는 영향을 받지 않았으며, 헤마토크리트에 의해서는 헤마토크리트가 낮을 때는 높은 혈당치를, 헤마토크리트가 높을 때는 낮은 혈당치를 나타냈다. The purpose of this study is to assess two monitors of blood glucose : One touch?? Ⅱ(Lifescan Inc.), which is using reagent strips by reflectance photometry and CompanionTM 2(Medisense Inc.), which is using electrochemical electrode method. Glucose was determined on capillary and venous blood samples from 70 adult diabetics by three different methodologies : One touchTM Ⅱ, CompanionTM 2 portable glucose meters (capillary) and the Hitachi 7150 analyzer(venous), which is using hexokinase method. In within-run precision studies for One touchTM Ⅱ and CompanionTM 2, coefficient variations (CVs, %) were 1.92∼5.09, 2.95∼4.79 at three different levels, respectively. In between-day percision studies for One touchTM Ⅱ and CompanionTM 2, CVs(%) were 4.69∼8.13, 3.14∼6.53 at three different levels, respectively. The linearity studies revealed r=0.9996 in One touch?? Ⅱ, r=0.9977 in CompanionTM 2, which were satisfactory. Correlation equation and coefficient for results by One touchTM Ⅱ and CompanionTM 2 vs those by hexokinase method(x) were : y=0.8087x-8.9566(r=0.9824) for One touchTM Ⅱ, y=0.8885x+4.4992(r=0.9807) for CompanionTM 2, which were satisfactory. The glucose levels were falsely high at low hematocrit concentration and falsely low at high hematocrit concentration in both monitors. The volume of blood applied to the test strips is not critical. We concluded that "One touchTM Ⅱ" using reagent strip by reflectance photometry and "Companion?? 2" using electrochemical electrode method is suitable for self monitoring of blood glucose.

자궁내막증 백서모형에서 Danazol과 Decapeptyl의 치료효과에 관한 연구

황선보,이민석,심재현,김동훈,배한익 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

본 연구의 목적은 장간막에 자궁내막 조직을 자가이식하여 수술적으로 유도된 자궁내막증 백서모형에서 자궁내막증의 치료를 위해 danazol과 decapetyl의 치료효과를 평가하는 것이다. 백서(n=48)들은 무작위적으로 정상대조군(n=12)과 수술적으로 유도된 자궁내막증군(n=36)으로 구분하였다. 후자는 비치료군과, danazol 치료군과 decapeptyl 치료군으로 세분하였다(각 군의 n=12). Danazol(8mg/일)은 수술후 4째주부터 구강을 통해 투여되었다. Decapeptyl(0.2mg/2주)은 근주하였다. 처음투약후 10일, 20일, 40일, 60일째에 각군의 2마리의 쥐를 희생시켰다. 저자들은 자궁내막증 이식체와 난소의 크기를 측정하여 다음의 결론를 얻었다. 1. 자궁내막증 이식체의 크기는 비치료군에 비해(3.91±1.08mm) danazol 치료군과 decapeptyl치료군에서 (각각2.28±0.17mm 과 2.78±0.97mm), 현저히 감소되었다(p<0.01). 2. danazol과 decapeptyl 두 치료군에서 장기치료가 단기치료보다 자궁내막증 이식체의 크기를 감소시키는데 더 효과적이었다(p<0.01). 3. 난소의 크기는 정상대조군과, 비치료군과 decapeptyl 치료군에서 유의하게 차이를 보이지 않은데 반해(각각 4.46±0.85mm, 4.77±0.82mm과 4.97±0.92mm), danazol 치료군에서는 유의하게 감소하였다(3.97±0.63mm) (p<0.63mm). 백서모형에서 수술적으로 이식된 자궁내막조직의 성장과 발달은 자궁내막증의 병인 이해와 신약개발에 유용한 연구모델을 제공할 수 있을 것 이다. The purpose of this study was to surgically induce endometriosis in the rat by autotransplanting endometrial tissue to the mesentery and to evaluate the efficacy of danazol and decapeptyl for the treatment of endometriosis. Rats(n=48) were randomly assigned to normal control group(n=12) and surgically induced endometriosis group(n=36). The latter was further divided into the untreated, danazol treated and decapeptyl treated groups (n=12 for each group). Danazol(8mg/day) was orally administrated from 4weeks after operation. Decapeptyl(O.2ml/2weeks) was injected intramuscularly. Two rats of each group are sacrificed on the 10th, 20th, 40th, 60th, 80th and lOOth day after the first drug administration. We measured sizes of endometriotic implants and ovaries. The results were as follows; 1. The sizes of endometriotic implants were significantly decreased(P<O.Ol) in the danazol and decapeptyl treated groups (2.28±O.71mm and 2.78±O.89mm, respectively) compared with untreated group(3.91±l.08mm). 2. Long term treatment was more effective than short term treatment in decreasing the size of endometriotic implants in both danazol and decapeptyl treated groups(P<O.Ol). 3. The size of ovaries was not significantly different among the normal control, untreated and decapeptyl treated groups(4.65±O.83mm, 4.77±O.82mm and 4.97±O.92mm, respectively) but was significantly decreased(P<O.Ol) in the danazol treated group(3.97±O.63mm). The successful growth and development of surgically transplanted endometrial tissue in the rat may offer an useful research model that can be used to understand pathogenesis of endometriosis and to explore new drug therapies.

퍼지 추론을 이용한 분류 벡터 양자화기의 성능 개선

황병하,김혜숙,서재성,박민식,곽훈성 全北大學校 1994 論文集 Vol.37 No.-

In Classified Vector Quantization(CVQ) for image coding, the block classification pondering the block characteristics will be the most important procedure if we consider system performance. But it does not take effect because classification of conventional CVQ followed act on a heuristic and statistics method. In order to solve the problem of conventional methods, i.e, for block classification effectively we propose a fuzzy inferencing algorithm to improve its performance. To do this, fuzzy rules must be generated in the first. Our rules are based on energy distribution in transform domain with respect to spatial variation. Ultimately fuzzy inferencing of this paper apply these fuzzy rules for efficient block classification on a input image. As a result of efficient classification, our proposed method shows that blocking effect is decreased and PSNR(peak to peak signal to noise ratio) is increased by about 1 ∼ 2 dB for 0.2 ∼ 0.6 bpp range, when the performance is compared with Aizawa's scheme^[9].

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.

중합효소연쇄반응법을 이용한 급성 치수 및 치근단 질환의 병원성 세균의 동정

김지훈,유소영,임선아,국중기,임상수,박슬희,황호길 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.2

The purpose of this study was to investigate the frequency of 7 putative pathogens in endodontic infections. The specimens were collected from infected pulpal tissue of patients who were referred for root canal treatment to the department of conservative dentisty, Chosun University. Samples were collected aseptically using a barbed broach and a paper point. The cut barbed broaches and paper points were transferred to an eppendorf tube containing 500 ml of 1 X PBS. DNAs were extracted from the samples by direct DNA extraction method using lysis buffer (0.5% EDTA, 1% Triton X-100). Identification of 7 putative pathogens was performed by PCR based on 16S rDNA. The target species were as follows: Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, and Treponema denticola. Our data revealed that the prevalence of P.endodontalis was found in 88.6% (39 / 54), P.gingivalis 52.3% (23 / 44), P.nigrescens 18.2%(8 / 44), P.intermedia 15.9% (7 / 44), B.forsythus 18.2% (8 / 44), A.actinomycetemcomitans 2.3% (1 / 44), T.denticola 25% (11 / 44) of the samples. The high prevalence of P. endodontalis and P.gingivalis suggests that they may play an important role in the etiology of endodontic infections.

복직근 및 박근 근피판술을 이용한 골반 및 서혜부 연부조직 결손의 재건

이재화,정윤규,김주봉,유대현,황성관,이훈범,김석원 大韓成形外科學會 1997 Archives of Plastic Surgery Vol.24 No.6

Many operations for the reconstruction of the pelvic area have been studied until now. The local muscle flap is often used for the closure of these complex wound. But, these local muscle flaps may not be available for reconstruction due to the factors of infection, radiation, and surgical trauma. Since rectus abdominis muscle and gracilis muscle were introduced, their use has been steadily increased for the reconstruction of soft tissue defect of the pelvic area. Inferiorly based rectus abdominis muscle flap and the gracilis muscle flap have been adopted for well-vascularized soft tissue coverage of the defect of pelvis and groin area. These techniques were utilized in seven patients. Of these patients, rectus abdominis muscle flap was used on four patients and three patients were treated with gracilis muscle flap. Among these, chronic osteomyelitis of pelvic bone has been managed with inferiorly based rectus abdominis muscle flap in three patients. No complication has been found. The mean postoperative follow-up period was 26 months, with a range of 12 to 39 months. In conclusion, these muscles are functionally expandable , and provide adequate tissue volume with sufficient blood supply away from the zone of injury, and readily transposed into the pelvis and groin area through subcutaneous tunnel, and serve as an antibiotic delivery system that aids in the eradication of infection. These findings suggest a reconstructive option for the defect of pelvis and groin, and/or bone infection in this area.

L1210 세포증식에 대한 Glycyrrhizin의 억제작용 기전

殷載淳,徐龍勳,權鎭,柳東和,吳贊鎬,蘇俊魯,全焄,黃甲洙 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The purpose of this research was to investigate the mechanism of inhibitory action of Glycyrrhizin(GZ) on the proliferation of mouse leukemia cell-line, L1210 cells. The cytotoxic activity was tested using a colorimetric tertrazolium assay(MTT assay), the apoptosis was tested using flow cytometry. Nitric oxide(NO) production form mouse peritoneal macrophage was tested using a Griess method and the phagocytosis of human polymorphonuclear cells was tested using a lucigenin chemiluminescence. GZ ingibited the proliferation of L1210, BALB/c 3T3 cells and mouse thymocytes at 50 ug/ml/ GZ did not affect nitric oxide production from mouse peritoneal macrophages in vitro, but ingibited nitric oxide production from lipopolysaccharide and y-interferon treated macrophages. Macrophages of GZ-administered mice accelerated NO production. The proliferation of L1210 cells apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. The apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. GZ increased the phagocytosis of human polymorphonuclear cells. These results suggest that GZ inhibit the proliferation of L1210 cells not only as a direct cytotoxic agent o tumor cells, but also by the enhancement of NO production and phagocytic activity.

고도정수처리 공정에서의 천연유기물질 특성변화 및 소독부산물 전구물질의 제거특성 평가

노재순,손희종,박은주,황영도,최동훈,배석문,신판세 慶星大學校 環境問題硏究所 2002 環境硏報 Vol.12 No.1

In this research the movement and apparent molecular weight distribution of hydrophilic/hydrophobic organic matters in advanced water treatment process were investigated using the raw water of Maeri area, located in downstream of Nakdong river, and the variation of the disinfection by-product formation potential was also investigated. In the raw water used in experiments, the content of BDOC (biodegradable dissolved organic carbon) in dissolved organic carbon (DOC) was about 39%, and this value is far above a normal, Pre-ozone treatment showed an increase of 40% in the BDOC content, and this means that biodegradability was remarkably increased by ozone treatment BDOC concentration in BAC treated water was 0.09 mg/L, which means 84% of BDOC removal rate compared with that in post-ozone treated water. The variation of chlorine disinfection by-product formation potential in each process unit of water treatment process was analyzed. TOXFP concentration in raw water was 449 ㎍/L, and the contents of THMFP and HAAFP among TOXFP were 24% and 23%, respectively. The removabilities of these materials BAC process had an order of TOXFP > HAAFP > THMFP. BAC process was less effective for THMFP than others. The distribution of hydrophilic organic matters were evaluated in each treatment process. Hydrophilic and hydrophobic matters in the raw water had a ratio of 50 : 50, and the ozone treatment decreased the content of hydrophobic matters. The BAC treatment made the ratio 85:15. These mean that the removal of hydrophilic matters is more difficult than that of hydrophobic materials in water treatment process. In addition the portions of humic and fulvic acid in hydrophobic matters were analyzed. Fulvic acid a large portion among hydrophobic matters in the raw water, and the portion was 78%. Ozone treatment was more effective on the removal of fulvic acid than humic acid. After BAC treatment, the concentration of residual fulvic acid was high than humic acid. Chlorine disinfection by-product formation for hydrophilic and hydrophobic mattrts was compared in each process unit. in the raw water TOXFP and THMFP formation for hydrophobic matter was higher, and HAAFP was highly formated for hydrophobic matter. After post-ozone or BAC treatment, chlorine disinfection by-product formation has high potential for hydrophobic matters. Comparing humic and fulvic acids, chlorine disinfection by-product formation potential was higher for humic acid than for fulvic acid in all processes. In the comparison of the reactivity with chlorine disinfection by-product per unit DOC, THMFP and DOXFP showed higher reactivity with hydrophobic matter than with hydrophilic matter in all processes, and HAAFP was highly reacitive with hydrophilic matter. Apparent molecular weight distributions of organic matters were investigated in each process step.The content of low molecular weight organic matters less than 1000 Da in DOC was high in all processes except raw water, but molecular weight organic matters more than 1000 da was higher than molecular weight organic matters less than 1000 Da in the formation potential of chlorine disinfection by-product.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

의료영상처리를 위한 고해상도 LCD 컨트롤러 개발

이채욱,황재석,박재훈 대구대학교 2005 대구대학교 학술논문집 Vol.1 No.1

In this paper we proposes the Implementation of Medical Image LCD Controller Using Multi-Image Equipment. Existent LCD is incongruent in resolution to use by medical. Therefore, proposed LCD is system that can do high definition display of suitable WUXGA (1900 * 1200) to medical. the existent LCD displays to detect TV signal or PC's signal, so developed LCD can detect PC's signal as well as TV signal. Five video signal is DVI, D-SUB, S-Video, Composite, Component and designed so that AUTO-SELECTION of five signal may be possible. This has advantage that detecting PC's signal as well as TV signal can make use of TV and monitor together doing unlike existent monitor. 본 논문에서는 의료영상처리를 위한 고해상도 LCD(Liquid Crystal Display) 컨트롤러의 구현 방법에 대하여 논한다. 기존의 LCD는 해상도 면에서 의료용으로 사용하기에는 부적합하다. 따라서 의료용에 적합한 WUXGA(1900 * 1200)의 고해상도 디스플레이를 해 줄 수 있는 고해상도 LCD를 개발하고자 한다. 또한 기존의 LCD는 TV신호 또는 PC신호만을 DETECTING해서 DISPLAY시켜주는 반면 본 LCD는 TV신호 뿐만 아니라 PC의 신호도 동시에 DECTECTING이 가능한 LCD 시스템이다. 5개의 영상신호는 PC신호인 DVI, D-SUB이며, TV 신호인 S-Video, Composite, Component이며 5개의 신호의 AUTO-SELECTION이 가능하도록 설계하였다. 이는 기존의 모니터와는 달리 TV신호 뿐만 아니라 PC의 신호도 DECTECTING함으로써 TV와 모니터를 같이 사용할 수 있는 장점을 가지고 있다.