항암제 AG60이 흰생쥐 위점막 상피세포의 DNA합성에 미치는 영향 : 자기방사법적연구

박경호,안의태,고정식,하상선,한영복,정영신,홍은경,유보림,김상건,이경영,강종구 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

산화동과 산화철을 이용한 색유약

심보경,이병하 明知大學校 産業技術硏究所 2006 産業技術硏究所論文集 Vol.25 No.-

Objectives of this study is no know an effect of coloring oxide such as CuO and Fe₂O₃ in the case of that has definite ratio on the basic components, and the an effect of colorizing agent in the present of SnO₂ and bone ash. On the three kinds of basic glaze(Iime, lime-barium and lime-magnesia glaze) the 1, 2, 3, 4, 5, 6, 7, 10 and 15% CuO and the 1, 3, 5 and 7% Fe₂O₃ were added, respectively, the changed color were detected when colorizing agents(the 1, 3 and 5% of SnO₂ and the 1, 3 and 5% of bone ash) were presented as firing at the 1250℃, oxidation and reduction firing, respectively.

미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자에서 혈장 Endothelin의 변화

서예경,정상원,남직화,신병호,전종민,김정국,김동희,하승우,김보완 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : 혈관내피세포에서 생성되는 endothelin(ET)는 강력한 혈관수축작용을 가진 펩타이드로 당뇨병 환자에서 혈관내피세포의 손상여부에 따라 혈중 농도가 증가할 수 있으며 혈관합병증과 관련이 있다는 보고가 있다. 그러나. 혈중 ET치의 상승과 당뇨병성 합병증의 상관성에 대해서는 논란이 많다. 이에 저자들은 인슐린 비의존성 당뇨병 환자에서 미세알부민뇨의 동반유무에 따른 혈중 ET치의 변화 및 혈장 ET치와 요중 알부민 배설률과의 상관성을 검토하였다. 대상 및 방법 : 인슐린 비의존성 당뇨병 환자중 미세알부민뇨를 보인 22명과 정상알부민뇨를 보인 43명을 대상으로 하였으며 혈장 ET를 측정하여 요중 알부민 배설률, 당뇨병성 망막증 및 당뇨병 관련인자들과의 관계를 비교하였다. 결과 : 당뇨병성 망막증이 합병된 군에서 망막증이 합병되지 않은 군에 비하여 혈장 ET치가 유의하게 높았던 반면 (각각 1.9±0.66, 1.5±0.76pg/㎖, p<0.05) 미세알부민뇨를 보인 군과 정상알부민뇨를 보인 군사이에 혈장 ET치는 통계학적으로 유의한 차이가 없었다(각각 1.9±1.14, 1.7±0.69pg/㎖). 또한 요중 알부민 배설률과 혈장 ET치 사이에 유의한 상관관계는 없었으며 미세알부민뇨를 동반한 인슐린 비의존성 당뇨병 환자군에서 혈장 ET치는 당뇨병의 관련인자들과도 유의한 상관관계가 없었다. 결론 : 인슐린 비의존 당뇨병 환자에서 혈장 ET치가 초기 당뇨병성 신증의 합병여부나 미세알부민뇨의 정도를 반영하기는 어려울 것임을 시사한다. Background : Endothelin(ET), a potent vasoconstritor peptide released from vascular endothelial cells, is known to be increased in diabetic patients with vascular damage and related with diabetic angiopathy. However, whether plasma ET levels may be related to diabetic complications is controversial. We studied the change of ET levels in non-insulin dependent diabetics with microalbuminuria and relation between ET and urinary albumin excretion rate. Methods : Plasma ET levels of 22 non-insulin dependent diabetics with microalbuminuria and 43 patients with normoalbuminuria were measured and relations of plasma ET to diabetic complications such as urinary albumin excretion rate, retinopathy and other associated factors were studied. Results : Plasma ET levels were significantly higher in patients with diabetic retinopathy than those without retinopathy(1.9 ± 0.66 vs 1.5 ± 0.76 pg/㎖. p<0.05). The difference, however, between diabetics with and without microalbuminuria was not significant(1.9 ± 1.14 vs 1.7 ± 0.69 pg/㎖). There were no correlations of plasma ET to urinary albumin excretion rate and to the other associated factors in non-insulin dependent diabetics. Conclusion : These results suggest that plasma ET levels may not seem to act as a predictor of diabetic nephropathy or microalbuminuria in non-insulin dependent diabetics.

황사 기간 동안에 어린이, 성인, 노인에서의 자각증상과 예방행동에 대한 조사연구

이보은,하은희,박혜숙,권지숙,신경림 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: Recently there are scientific evidences for the health effects of Asian dust-storms. Particularly in 2002, the daily average of PM_(10) exceeded 600 and 700 ㎍/㎥ in March and April respectively. We examined the effects of Asian dust-storms on perceived symptoms and preventive behaviors among children, adults, and elders. Method: The subjects of this study were 425 children, 444 adults and 60 elders. A questionnaire survey was carried out on the children and the adults by teachers and on the elders by interviewers to obtain information on demographic variables, perceived symptoms and preventive behaviors, previous respiratory disease, environmental tobacco smoke, and proximity between the house and the road during Asian dust-storms. We analyzed using descriptive statistics, X_(2) test and multiple logistic regression models. After adjustment for potential confounders, we estimated the odds ratios and 95% confidence intervals of the children and the elders for perceived symptoms and preventive behaviors. Result: The elders had a significantly higher prevalence of respiratory symptoms than the other groups. The children had a significantly higher prevalence than the adults in getting URI (1.51), coughing (1.68), rhinorrhea (1.46), fever (2.39) and medication for allergy or asthma (1.90). The children had better behaviors than the elders in closing windows (3.57), taking less outdoor recreational activity (2.59) and staying home (2.60).

일부 여대생의 스트레스와 철분 영양상태에 관한 연구

승정자,하보경,김미현,최선혜 숙명여자대학교 건강. 생활과학연구소 1999 生活科學硏究誌 Vol.14 No.1

The purpose of this study was to evaluate the iron status of 39 female adult who divided into five groups according to the stress condition. The iron status was evaluated based on dietary intake and blood analysis. The average age of the subjects was 23.10years, and the average score of BMI was 20.06kg/m2. The proportion of subjects in each stress condition was : 2.56%(1) in A1type(the group who easily get disease associated with stress), 64.10%(25) in A2type(condition susceptible to the disease associated with stress), 30.77%(12) in ABtype(middle type of A and B), 2.56%(1) in B2type(appropriate stressed condition) and 0%(0) in B1type(risk free to the disease associated with stress). The average daily intake of energy in study subjects was 2,399kcal, ti caloric ratio of carbohydrate, protein, fat was 59.90 : 14.39 : 25.71, respectively. There was no significant difference in anthropometric measurements, dietary intake status, hematologic indices of iron status between high stress group(A1type and A2type) and low stress group(ABtype and B2type). To compare the iron stores between high stress group and low stress group, subjects in each group were devided into iron 0 mg, 250 mg, 500 mg group. Stress level did not affect iron stores although in both groups, number of subjects in iron 0 mg group were higher than those of iron 250 mg and 500mg groups. Correlation coefficients between anthropometric measurements, nutrient intake status, hematologic indices of iron status and stress score of subjects did not reveal any significance. Therefore, the results suggest that psychological stress did not affect the iron status in normal female adult. However, be more systematic study on stress and the iron status is required in the future.

성인에서 장기간 Furosemide오용과 연관된 신수질 석회화

김윤구,이윤하,이규백,장세호,김대중,오하영,김보현,김미경,조윤숙 대한신장학회 1996 대한신장학회잡지 Vol.15 No.4

Streptozotocin-당뇨병 백서에서 당뇨병 이환기간에 따른 대동맥의 이완능 저하 및 Oxygen-Free Radical에 의한 이완능의 손상정도

김정국,김신우,김수동,서예경,하승우,김보완,백운이,권삼,강승완 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

목적: 당뇨병의 이환기간이 혈관의 내피세포 의존성 및 비의존성 이완능의 저하에 미치는 영향과 이환기간에 따라 oxygen-free radical(OFR)에 의한 동맥이완능의 손상정도에 차이가 있는지를 알아보고자 하였다. 방법: Streptozotocin(STZ)으로 당뇨병이 유도된 3 주 및 10주째의 백서군과 연령이 비슷한 정상 대조백서군의 적출 대동맥환을 norepinephrine으로 수축시킨 상태에서 내피세포 의존성 이완제인 acetylcholine(ACh) 및 비의존성 이완제인 nitroglycerine(NTG)에 대한 이완능을 즉정하여 비교 관찬하였다. 또한 xanthine과 xanthine oxidase의 혼합으로 생성된 H₂O₂에 대동맥환을 노출시킨뒤 역시 ACh과 NTG에 대한 이완능의 손상정도를 비교하였다. 결과: 3주및 10주째 당뇨병 백서의 대동맥환은 모두 대조군 백서의 대동맥환에 비해 ACh에 대한 이완능이 유의하게 감소하였으며 10주째 당뇨병 백서군에서의 ACh에대한 이완능의 저하는 3 주째 당뇨병 백서군에 비해 유의하게 심하였다.H₂O₂에 노출시킨 뒤 ACh에대한 이완능은 3주 및 10주 째 당뇨병 백서군에서 모두 대조군에 비해 유의하게 심한 손상을 보였으나 3주와 10주째 당뇨병 백서군 사이의 손상정도의 차이가 통계학적으로 유의하지는 않았다. 한편 3주 및 10주째 당뇨병 백서군 모두 NTH에 대한 대동맥환의 이완능은 대조 백서군과 별다른 차이가 없었으며 또 H₂O₂에 노출시킨 뒤에도 NTG에 대한 이완능의 손상은 관찰되지 않았다. 결론: STZ-당뇨병백서에서 대동맥의 이완능은 당뇨병에 이환된지 3 주째에 이미 ACh에 대한 내피세포 의존성 이완능이 선택적으로 저하되며 당뇨병의 이환기간이 길수록 이완능의 저하가 심하였고, OFR에 의한 동맥 이완능의 손상도 내피세포 의존성 이완능에만 선택적으로 나타나나 이환기간에 따라 OFR에 의한 이완능 손상정도에는 뚜렷한 차이가 없음을 시사하고 있다. Objectives: Diabetes mellitus produces histological evidence of endothelial as well as changes in vascular function in experimental animal. Oxygen free radicals interfere with of destroy endothelium-dependent relaxation in normal and diabetic blood vessels. The present study was investigated whether the duration of diabetic state can affect the severity of impairment in endothelium-dependent and/or independent relaxation and the sensitivity to oxygen free fadical (OFR)-induced damage in diabetic rat vasculature. Methods: The relaxative responses of aortic ring precontracted with norepinephrine in vitro to cumulative concentrations of acetylcholine (ACh) and nitroglycerine (NTG) were conducted in 3- and 10- week streptozotocin (STZ)-diabetic rats and agematched controls. After exposure to H₂O₂generated by mixing xanthine and xanthine oxidase, the degree of impairment in relaxative responses to ACh and NTG were also compared between 3- and 10-week diabetic rats. Results: The relaxative responses of aortic rings produced by cumulative concentrations of ACh were significantly lower in both 3- and 10-week diabetic rats than in the age-matched controls. The impairments of relaxative responses to ACh in 10-week diabetic rats were significantly greater than those in 3-week ones. After H₂O₂ exposure, the impairments of relaxative responses to ACh in 3- and 10-week diabetic aortas was also significantly greater than those in the control in the control ones. However, the severity of H₂O₂-induced damage in 10-week diabetic aortas was not significantly different than that in 3-week ones. In contrast, aortic rings of 3- and 10-week diabetic rats could still be fully relaxed by NTG, even after exposure to H₂O₂. Conclusion: These findings suggest that in SRZ-diabetic rat aortas selective impairment of endothelium-dependent relaxation to Ach can be found early even over a 3-week period after the induction of diabetes and the impairment may be greater in those with the longer duration of the diabetic state and that the impairment of the vascular relaxation by OFR is also selective to endothelium-dependent response but the sensitivity of OFR-induced damage may not be related to the duration of the diabetic state.

중 고등학교 수학교육 개선을 위한 연구

邊龍聖,金東碩,邊東壽,崔雲行,洪慶和,鄭寶鉉 弘益大學校 1991 弘大論叢 Vol.23 No.2

A great many students are working for their mathematics learning in secondary schools and nevertheless its learning effect is rather low. This phenomenon is largely due to a biased social recognition. Furthermore the educational environment including big class size and ability differences enlarge such a difficulty. This study aims at seeking an improving device to enhance learning efficiency under the circumstances. Questionaires about problem finding of teachers educational activity and mathematics teaching were distributed and collected. Data were analyzed to establish a desirable mathematics education and teaching. Devices for individualized instruction and learning tasks and evaluation methods were proposed. This recommendations are supposed to be an improving way for students' mathematics learning. In relation to this result, a further study is strongly required and adminstrative supports by government are badly needed to achieve such a goal.

당뇨병 백서의 대동맥에서 열 자극이 혈관 운동성에 미치는 영향

구성모,이순희,한정훈,정기영,김인겸,김정국,하승우,김보완 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5

연구배경:열자극은 열자극 단백질의 생성을 유도하여 유해한 자극에 대한 세포내 반응을 유도하는 것으로 알려져 있다. 열자극 단백질은 여러 조직에서 발현되며 일부 열자극 단백질은 산화 스트레스에 대해 세포를 보호하는 기능을 나타내기도 한다. 최근 보고에 의하면, 일부 열자극 단백질은 혈관운동성과 관련이 있다고 한다. 당뇨병은 수축물질에 대한 과다한 수축과 이완물질에 대한 이완능의 저하를 특징으로 하는 혈관 운동성의 장애를 초래하는 대표적인 질환이다. 이런 혈관운동성의 장애는 당뇨병성 혈관병증의 병인에 중요한 요소이다. 그러나 고혈당의 조건에서 혈관 운동성에 대한 열자극의 효과에 대해서는 잘 알려져 있지 않으며 그 기전에 대해서도 밝혀진 것이 없다. 이에 스트렙토조토신으로 당뇨병을 유발한 백서 대동맥에서 열자극이 혈과운동성에 미치는 영향에 대하여 연구하였다. 방법:당뇨병 백서 대동맥을 적출하여 대동맥환을 준비하고 장치한 후 42도에서 45분간 열자극을 가한군과 열자극을 가하지 않은 군사이의 혈관 수축물질에 대한 수축반응과 이와물질에 대한 이완반응을 측정하였다. 혈관반응을 측정한 후 대동맥환을 얼리고 열자극단백질 HSP 70의 발현을 조사하였다. 결과:당뇨병 백서와 정상 대조군 백서의 대동맥에서 KCI과 phenyephrine에 대한 혈관 수축반등은 열자극을 준 군에서 주지 않은 군에 비해 현저하게 증가하였다. 열자극은 당뇨병과 정상 대조군의 대동맥에서 혈관수축반응의 증가 뿐만 아니라 HSP 70의 발현을 의미있게 증가시켰다. 정상 대조군의 대동맥에서 아세틸콜린에 대한 이완반응은 열자극을 준 군과 주지 않은 군사이에 통계학적 유의한 차이는 없었다. 반면에 당뇨병 백서의 대동맥에서는 열자극을 준 군이 주지않은 군에 비해 아세틸콜린에 대한 이완반응이 유의하게 감소하였다. 결론:이상의 결과는 당뇨병과 정상쥐에서 열자극에 대한 혈관 수축반응의 증가는 HSP 70의 발현을 유도하여 일어날 수 있음을 시사하여 당뇨병군에서 정상 대조군에 비해 열자극에 대한 이완반응이 감소하는 기전은 많은 연구가 되어야 할 것으로 생각된다. Background: Heat shock has been known to change cellular response to noxious stimuli by inducing heat shock proteins(HSP). HSP are expressed in many tissues, and increased expression of some HSP enhances the survival of cells exposed to oxidative stress. Recently, Some HSP are known to associate with vascular reactivity. Under diabetic conditions, there is a abnormal vascular reactivity to relaxing or contracting factors. Abnormal vascular response to some stimuli is an important role in the development of diabetic complications. However, the effects of heat shock on the vascular reactivity in diabetic condition is unclear. Therefore, we investigated effects of heat shock on the vascular reactivity in isolated aorta of streptozotocin-induced diabetic rats. Methods: After mounced in organ bath, aortic ring preparations were exposed to 42℃ for 45 minutes followed by being subjected to contraction and relaxation in 4 hours. Tissues were frozen for measurement of HSP 70 and phosphorylation of myosin light chain after functional study. Results: Heat shock not only increased expression of HSP70 in rat aorta but also augmented contraction to KCI and phenylephrine in the aorta of control and diabetic rats(p<0.05). Relaxation responses to acetylcholine (Ach) were not changed in the aorta of control rats with and without heat shock for 45 minutes. However, heat shock for 45 minutes decreased relaxative responses to Ach in the aorta of diabetic rats compared to those in the aorta of control rats. Conclusion: This result suggests that heat shock increases vascular contractility in the aorta of diabetic and control rats through the induction of HSP70 while heat shock seems to decrease relaxative response in the aorta of diabetic rats compared to control rats(p<0.05). Whether heat shock impaired relaxative response in the aorta of diabetic rats deserves additional studies (J Kor Diabetes Asso 25:343~353, 2001).

Improved Retinoids Production from Metabolically Engineered Escherichia coli with a Two-phase Culture System

Bo-Kyung HA,Hui-Jeong JANG,Sang-Hwal YOON,Zhou JIA,Chong-Long WANG,Deok-Kun OH,Seon-Won KIM 한국생물공학회 2012 한국생물공학회 학술대회 Vol.2012 No.4