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Production of Vanillin from Ferulic Acid Using Recombinant Strains of Escherichia coli
Yoon Sang-Hwal,Li Cui,Lee Young-Mi,Lee Sook-Hee,Kim Sung-Hee,Choi Myung-Suk,Seo Weon-Taek,Yang Jae-Kyung,Kim Jae-Yeon,Kim Seon-Won The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.4
Vanillin is one of the world's principal flavoring compounds, and is used extensively in the food industry. The potential vanillin production of the bacteria was compared to select and clone genes which were appropriate for highly productive vanillin production by E. coli. The fcs (feruloyl-CoA synthetase) and ech (enoyl-CoA hydratase/aldolase) genes cloned from Amycolatopsis sp. strain HR104 and Delftia acidovorans were introduced to pBAD24 vector with $P_{BAD}$ promoter and were named pDAHEF and pDDAEF, respectively. We observed 160 mg/L vanillin production with E. coli harboring pDAHEF, whereas 10 mg/L of vanillin was observed with pDAHEF. Vanillin production was optimized with E. coli harboring pDAHEF. Induction of the fcs and ech genes from pDAHEF was optimized with the addition of 13.3 mM arabinose at 18 h of culture, from which 450 mg/L of vanillin was produced. The feeding time and concentration of ferulic acid were also optimized by the supplementation of $0.2\%$ ferulic acid at 18 h of culture, from which 500 mg/L of vanillin was obtained. Under the above optimized condition of arabinose induction and ferulic acid supplementation, vanillin production was carried out with four different types of media, M9, LB, 2YT, and TB. The highest vanillin production, 580 mg/L, was obtained with LB medium, a 3.6 fold increase in comparison to the 160 mg/L obtained before the optimization of vanillin production.
Yoon, Sang-Hwal,Lee, Young-Mi,Kim, Ju-Eun,Lee, Sook-Hee,Lee, Joo-Hee,Kim, Jae-Yean,Jung, Kyung-Hwa,Shin, Yong-Chul,Keasling, Jay D.,Kim, Seon-Won Wiley Subscription Services, Inc., A Wiley Company 2006 Biotechnology and bioengineering Vol.94 No.6
<P>To increase expression of lycopene synthetic genes crtE, crtB, crtI, and ipiHP1, the four exogenous genes were cloned into a high copy pTrc99A vector with a strong trc promoter. Recombinant Escherichia coli harboring pT-LYCm4 produced 17 mg/L of lycopene. The mevalonate lower pathway, composed of mvaK1, mvaK2, mvaD, and idi, was engineered to produce pSSN12Didi for an efficient supply of the lycopene building blocks, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Mevalonate was supplied as a substrate for the mevalonate lower pathway. Lycopene production in E. coli harboring pT-LYCm4 and pSSN12Didi with supplementation of 3.3 mM mevalonate was more than threefold greater than bacteria with pT-LYCm4 only. Lycopene production was dependent on mevalonate concentration supplied in the culture. Clump formation was observed as cells accumulated more lycopene. Further clumping was prevented by adding the surfactant Tween 80 0.5% (w/v), which also increased lycopene production and cell growth. When recombinant E. coli harboring pT-LYCm4 and pSSN12Didi was cultivated in 2YT medium containing 2% (w/v) glycerol as a carbon source, 6.6 mM mevalonate for the mevalonate lower pathway, and 0.5% (w/v) Tween 80 to prevent clump formation, lycopene production was 102 mg/L and 22 mg/g dry cell weight, and cell growth had an OD<SUB>600</SUB> value of 15 for 72 h. © 2006 Wiley Periodicals, Inc.</P>
Production of vanillin by metabolically engineered Escherichia coli
Yoon, Sang-Hwal,Li, Cui,Kim, Ju-Eun,Lee, Sook-He,Yoon, Ji-Young,Choi, Myung-Suk,Seo, Weon-Taek,Yang, Jae-Kyung,Kim, Jae-Yeon,Kim, Seon-Won Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
E. coli was metabolically engineered to produce vanillin by expression of the fcs and ech genes from Amycolatopsis sp. encoding feruloyl-CoA synthetase and enoyl-CoA hydratase/aldolase, respectively. Vanillin production was optimized by leaky expression of the genes, under the IPTG-inducible trc promoter, in complex 2YT medium. Supplementation with glucose, fructose, galactose, arabinose or glycerol severely decreased vanillin production. The highest vanillin production of 1.1 g 1^(-1) was obtained with cultivation for 48 h in 2YT medium with 0.2% (w/v) ferulate, without IPTG and no supplementation of carbon sources.
Production of Vanillin from Ferulic Acid Using Recombinant Strains of Escherichia coli
Yoon, Sang-Hwal,Li, Cui,Lee, Young-Mi,Lee, Sook-Hee,Kim, Sung-Hee,Choi, Myung-Suk,Seo, Weon-Taek,Yang, Jae-Kyung,Kim, Jae-Yeon,Kim, Seon-Won Plant molecular biology and biotechnology research 2005 Plant molecular biology and biotechnology research Vol.2005 No.
Vanillin is one of the world's principal flavoring compounds, and is used extensively in the food industry. The potential vanillin production of the bacteria was compared to select and clone genes which were appropriate for highly productive vanillin production by E. coli. The fcs(feruloyl-CoA synthetase) and ech (enoyl-CoA hydratase/aldolase) genes cloned from Amycola-topsis sp. strain HR104 and Delftia acidovorans were introduced to pBAD24 vector with P_(BAD) promoter and were named pDAHEF and pDDAEF, respectively. We observed 160 mg/L vanillin production with E. coli harboring pDAHEF, whereas 10 mg/L of vanillin was observed with pDDAEF. Vanillin production was optimized with E.coli harboring pDAHEF. Induction of the fcs and ech genes from pDAHEF was optimized with the addition of 13.3 mM arabinose at 18 h of culture, from which 450 mg/L of vanillin was produced. The feeding time and concentration of ferulic acid were also optimized by the supplementation of 0.2% ferulic acid at 18 h of culture, from which 500 mg/L of vanillin was obtained. Under the above optimized condition of arabinose induction and ferulic acid supplementation, vanillin production was carried out with four different types of media, M9, LB, 2YT, and TB, The highest vanillin production, 580 mg/L, was obtained with LB medium, a 3.6 fold increase in comparison to the 160 mg/L obtained before the optimization of vanillin production.
( Jun Sang Ahn ),( Jung Suh Shin ),( Min Ji Kim ),( Gi Hwal Son ),( Eung Gi Kwon ),( Jae Yoon Shim ),( Il Young Kim ),( Sung Myoun Cho ),( Sang Rae Cho ),( Byung Ki Park ) 한국축산학회(구 한국동물자원과학회) 2019 한국축산학회지 Vol.61 No.3
This study aimed to investigate the effects of temperature and retention time of the pressurized steam chamber on the ruminal fermentation characteristics and nutrient degradability of corn flakes in three Korean native Hanwoo cows and three Holstein cows implanted with a ruminal fistula. Corn kernels were categorized into 13 groups based on the chamber temperature (range, 100°C-116°C) and retention time (range, 700-950 s). The pH value was lowest in T1 regardless of breed. Propionate concentration was the highest in T2 (p < 0.05). Total-volatile fatty acid (VFA) concentration was slightly but not significantly greater in T2 than in other conditions. Dry matter (p < 0.05), starch, and crude protein (p < 0.05) degradability were the highest in T1. At different incubation times and with different breeds, dry matter, starch, and crude protein degradability of corn flakes were the highest in T1. Thus, the present results indicate that the optimal temperature and retention time of the pressurized steam chamber should be 100°C-105°C and 700-720 s.
Farnesol production from <i>Escherichia coli</i> by harnessing the exogenous mevalonate pathway
Wang, Chonglong,Yoon, Sang‐,Hwal,Shah, Asad Ali,Chung, Young‐,Ryun,Kim, Jae‐,Yean,Choi, Eui‐,Sung,Keasling, Jay D.,Kim, Seon‐,Won Wiley Subscription Services, Inc., A Wiley Company 2010 Biotechnology and bioengineering Vol.107 No.3
<P><B>Abstract</B></P><P>Farnesol (FOH) production has been carried out in metabolically engineered <I>Escherichia coli</I>. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, <I>E. coli</I> was metabolically engineered to overexpress <I>ispA</I> and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two‐phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant <I>E. coli</I> harboring the pTispA and pSNA plasmids for <I>ispA</I> overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from <I>E. coli</I> without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by <I>E. coli</I> while no detectable FOH production was observed in the endogenous MEP pathway‐only control. Biotechnol. Bioeng. 2010;107: 421–429. © 2010 Wiley Periodicals, Inc.</P>
모듈러 유닛 공장 생산성 향상을 위한 철골 부재 표준화에 관한 연구
장활제(Jang, Hwal-Je),윤석운(Yoon, Seok-Woon),이정훈(Lee, Jeong-Hoon),이현수(Lee, Hyun-Soo),박문서(Park, Moon-Seo),현호상(Hyun, Ho-Sang) 대한건축학회 2016 대한건축학회논문집 Vol.32 No.1
The purpose of this study is to propose of standardized modular steel structure unit for productivity enhancement of productivity in modular factory. For the verification, comparison review in various points of view between existing modular steel structure unit and standardized modular steel structure unit based on recently completion of modular system buildings. This research is suggestion of enhancement of standardized steel structure"s members for better production workability. The research is based on (1) steel structure process which is most high rate (approximately 30%) of modular unit manufacturing process and (2) prototype unit size of 3M × 6M × 3.2M(H) among the most used in recently project. For the verification, comparison analysis between existing steel structure unit and standardized steel structure unit"s manufacturing process. The research result, one modular unit of standardized steel structure manufacturing process is reducting (1) 250,042won production cost, (2) 3 hours and 49 minute of production time. Also, (3) increase the factory area availability of 203.84㎡.