몇가지 호르몬이 부신이 적출된 흰쥐의 장점막배상세포에 미치는 영향

안의태,김강석,오영희 최신의학사 1972 最新醫學 Vol.15 No.9

This experiment was performed in order to study the influence of adrenalectomy on the intestinal goblet cells of rats and the effects of some hormones on the cells of the adrenalectomized rats. Healthy adult rats weighing about 200g were divided into three groups: normal, adrenalectomized, and hormone-treated. Adrenalectomy was done under ether anaesthesia. The adrenalectomized rats were killed on the 3rd and 7th day after the operation and compared with those of sham operated. The hormone-treated group was further divided into adrenocorticotropic hormone, cortisone, deoxycorticosterone acetate, and epinephrine sub-groups, and each kind of hormone was injected subcutaneously every day from the 8th post-operative day with a dose of 1.25 1U, 25 mg, I mg and 0. 1 mg per kg body weight respectively. The hormone-treated animals were sacrificed on the 3rd and 7th day from the beginning of the injection. All findings of hormone-treated groups were compared with those of saline injected group. Duodenal and colonic mucosae of experimental rats were fixed in 10% neutral formalin solution, j embedded in paraffin wax, sectioned in 6 p thickness, and stained with hematoxylin-eosin stain for general findings and with periodic acid-Schiff reaction, alcian blue pH 2. 5, alcian blue pH 1. 0 and combined alcian blue-PAS for the mucosubstance of goblet-cells. Observed results were as follows: 1. Adrenalectomy caused the decrease of mucosubstance in volume, coagulation or dissolution of mucosubstance of goblet cells. Histochemically, sulfated mucosubstance most sensitively responsed. 2. Adrenocorticotrophic hormone did not influence the mucosubstance of intestinal goblet cells in the adrenalectomized rats. 3. Administration of cortisone, deoxycorticosterone acetate or epineprine to the adrenalectomized rats caused an increase in volume of the neutral and acidic mucosubstances of intestinal goblet cells

의과대학 학생의 뇌기능 특성화 경향

안의태,김민정,박경호,박대균,고정식 대한체질인류학회 2006 해부·생물인류학 (Anat Biol Anthropol) Vol.19 No.4

In this study, cerebral functional laterality patterns of medical students in the year 2005 were compared with those in the year 1995. Questionnaires on the behavior patterns were asked, and the laterality patterns were classified as right hemispheric (R)-balanced hemispheric (B)-left hemispheric (L). 385 students were studied (210 male and 175 female). Of the 3 categories, male students showed the patterns of R (42.8%), B (31.9%) and L (25.3%). Female students showed R (45.2%), B (30.9%) and L (23.9%). As the above result shows, laterality patterns of male and female were similar. The above data were compared with the another data in the article reported in 1995. Previous report showed that R (63.5%), B (24.9%) and L (11.6%) in the male students, and R (49.0%), B (22.4%) and L (28.6%) in female students, respectively. From the above results, It was suggested that cerebral laterality patterns of medical students in the year 2005 shifted toward left, but it still remained 42~45% in right hemispheric as contrast to 24~25% in left hemispheric. Hemispheric shift was interpreted as that, it may be the result of student’s adaptative or competitive activities in the fast changing social environment. 특정 학생집단의 전반적인 뇌기능 성향을 아는 것은 교육적으로도 큰 의의가 있다고 생각된다. 이 연구 는 의과대학 학생들에게 설문형식을 통해서 뇌기능의 편재성향을 알아보고, 10년 전에 시행한 연구 결과와 비교 하여 어떤 변화를 보이는지 알아보았다. 35개 설문 문항에 대해 오른쪽 뇌 성향과 왼쪽 뇌 성향에 맞는 대립 문항을 설정하였으며, 각 문항에서 한 개 씩만 택하게 한 다음, 강한 오른쪽 뇌형(ER, extremely right), (보통)오른쪽 뇌형(R, right), (오른쪽과 왼쪽) 균형 뇌 형(B, balanced), (보통) 왼쪽 뇌형(L, left), 강한 왼쪽 뇌형(extremely left, EL)으로 구분하여 편재성의 정도를 구별 하였으며 분석 결과는 다음과 같았다. 총 385명(남학생 210명, 여학생 175명)의 설문에서 남학생집단은 오른쪽 42.8%-균형 31.9%-왼쪽 25.3%였고, 여학생은 오른쪽 45.2%-균형 30.9%-왼쪽 23.9%로서 남학생과 여학생의 뇌기능 편재성이 매우 유사하였다. 1995 년 의과대학 학생을 대상으로 조사한 결과에서는 남학생은 오른쪽 63.5%, 균형 24.9%, 왼쪽 11.6%였고, 여학생은 오른쪽 49.0%, 균형 22.4%, 왼쪽 28.6%의 분포를 보여서 남학생의 오른쪽 뇌 편재성이 특히 강했었다. 이상의 결과를 종합해 보면 의과대학생들의 뇌기능 편재성은 1995년에는 오른쪽 뇌형이 압도적으로 많았으나, 10년이 지난 현재에는 그 경향이 많이 완화되었다고 생각되며, 이와 같은 변화는 그동안 빠르게 진행되어온 사회 변화와 더욱 심화된 경쟁사회 등의 상황과 관련이 있지 않을까 추측 된다.

19. 전공별 대학생집단의 뇌기능특성화 경향에 대한 연구

안의태,양남길,최기수 대한체질인류학회 1990 대한체질인류학회 학술대회 연제 초록 Vol.33 No.-

머리에 방사선 조사를 받은 흰쥐 신경뇌하수체의 미세구조 연구

안의태,김표년,양남길,고정식,박경호,김진국 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.1

방사선이 뇌실막세포의 미세구조에 미치는 영향

안의태,조휘동,김진국,박경호,고정식,Ahn, E-Tay,Cho, Hwee-Dong,Kim, Jin-Gook,Park, Kyung-Ho,Ko, Jeong-Sik 한국현미경학회 1999 Applied microscopy Vol.29 No.1

머리 부분에 많은 양의 방사선을 조사 받은 흰쥐 뇌실막세포의 미세구조에 대하여 연구하였다. 체중 $200\sim250g$의 흰쥐를 실험동물로 사용하였고, 방사선 발생장치로는 Mitsubishi linear accelerator (ML-4MV)를 이용하였다. 실험군의 흰쥐는 sodium thiopental로 마취시킨 후 머리부분이 조사구역 $(30cm\times30cm)$ 안에 들도록 눕힌 후, 조사거리 80cm, 조사 깊이 1.2 cm의 조건에서 200 rad/min의 속도로 연속 조사하였다. 실험군에 따라 3,000 rad 또는 6,000 rad를 조사시킨 후 각각 6시간, 2일, 6일 후에 동물들을 희생시켰다. 희생시에는 마취된 흰쥐의 가슴을 열고 심장을 통한 관류고정을 시행하였고, 관류고정액은 1% glutaraldehyde-1% paraformaldehyde액을 사용했다. 고정된 뇌에서 가쪽뇌실벽 일부를 메어 관류고정액과 같은 고정액에 다시 고정한 후, 2% osmium tetroxide 액으로 이차고정 하였고, 이후 통상적인 방법으로 전자현미경 절편제작 및 염색과정을 거친 후 전자현미경으로 관찰한 결과 다음과 같은 결론을 얻었다. 1. 방사선조사후 6시간군부터 뇌실막세포는 종창현상을 보였고 섬모의 배열이 흐트러졌으며 부분적으로 세포질이 뇌실공간으로 돌출하였다. 2. 방사선조사후 2일군부터는 뇌실막세포의 종창현상이 심하며 뇌실막밑조직의 부종이 심했다. 3. 뇌실막세포의 돌출부분 세포질에는 섬모바닥체, 사립체, 세포질세망들이 들어 있었다. 4. 방사선조사군에서는 확장된 뇌실막세포사이공간을 통하여 뇌실막밑층의 축삭성분 등이 뇌실속 까지도 돌출하였다. 이와 같은 결과로 보아 방사선조사에 의해 뇌실막세포에는 심각한 형태학적 변화가 초래되며, 이로써 뇌실질과 뇌척수액사이의 대사관문이 교란될 것으로 생각된다. Ultrastructure of the ependymal cells of X-irradiated rats on their head were studied. Rats weighing $200\sim250gm$ were X-irradiated on their head and neck areas. Total exposures were 3,000 rads or 6,000 rads depending on experimental groups. And irradiated rats were sacrificed on 6 hours, 2 days and 6 days following the radiation exposures. Animals were perfused through the heart with 1% glutaraldehyde-1% paraformaldehyde solution, under ether-anesthesia. The tissues from the wall of lateral ventricles were fixed in the 2% osmium tetroxide solution. The results observed with electron microscope were as follow: 1. In 6 hours group, many ependymal cells were swelled, luminal portions of cytoplasms of some cells protruded into the ventricular lumen, and many cilia were lost or irregularly altered. 2. In 2 days group, ependymal cells were swelled more severely and subependymal edema were pronounced. 3. Protruded cytoplasm contained usually basal bodies of cilia, groups of mitochondria, endoplasmic reticula , etc. 4. Following X-irradiations, some protruded masses contained neural elements including the axon terminals with dense core vesicles. Axons and axon terminals were also found in the enlarged intercellular spaces among ependymal cells. From the above results, the heavy irradiation on the head area of the rat induced alteration of the ependymal cells lining the lateral ventricle. Hence the ependymal functions of selective barrier, protective barrier, and metabolic barrier could be altered following X-irradiation on the head.

Ehrlich 암세포에 대한 Acriflavine-Guanosine 복합제 (AG60)의 항암효과 -광학현미경적, 자기방사법적 및 전자현미경적 연구-

안의태,고정식,김형진,김상건,이경영,강종구,유보림,정영신,홍은경,한영복 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2

연령증가에 따른 생쥐 혈액-가슴샘 관문의 미세구조

안의태,최석봉,고정식,경홍기,한영복 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.4

뇌 손상에 따른 신경아교세포의 가변성 반응

안의태,고정식,박경호 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.5

Acriflavine과 Guanosine 복합투여가 생쥐가슴샘겉질의 미세구조에 미치는 영향

안의태,박경호,고정식,정기수,한영복,흥은경,정영신,유보림,김상건 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.6

탈수상태가 흰쥐의 장관점액분비세포에 미치는 형태학적 영향

안의태,정성영 최신의학사 1971 最新醫學 Vol.14 No.10

In order to study on the morphological and histochemical changes of -mucus secretory cells in the rat intestinal mucosa following acute dehydration and during convalescence, this experiment was performed. Fifty healthy adult female rats weighing about 200gm were divided into normal, dehydrated, and convalescent groups. Dehydrated rats obtained by absolute restriction of drinking water were sacrificed at 1, 2, 3 and 5days, and convalescent ones obtained by permission of drinking water ad libitum after 5 days-restriction sacrificed at 1, 2, 3, 5 and 10 days. All of experimental rats were kept under constant room temperature and humidity. Specimens of duodenum and colon of experimental rats were fixed in 10% neutral buffered formalin solution, embedded in paraffin wax, sectioned in 6 micra thickness, and stained with hematoxylin-eosin stain and alcian blue (pH 2.5)-periodic acid-Schiff reaction for mucous substances. Observed results were as follows: 1. Atrophy, of the mucous membrane, severe desquamation of the surface epithelial cells, and increase of round cells in the lamina propria were seen in the duodenum and colon following acute dehydration. Severity of damage was lesser in the colonic mucosa than in the duodenal mucosa, and recovery of the colonic mucosa was faster than that of the duodenal ones in reconvalescent stage. 2. In the normal duodenal mucosa, blue or blue-purple mucus coating was seen, the goblet cells showed various tint from blue to violet according to their level in villi and, in general, alcianophilia predominated in the cells located at the tip or sides of villi, and the Brunner's gland cells revealed alcianophilia in the luminar cytoplasm. Under acute dehydrated condition, decrease of alcianophilia in the goblet cells and Brunner's gland cells and decrease of number of goblet cells were distinct, and during the convalescent stage, the recovery of number and mucus content of the goblet cells was prior to