Ginsenoside Rg_3 Enhances Large Conductance Ca^(2+)-Activated Potassium Channel Currents: A Role of Tyr360 Residue

최선혜,Tae-Joon Shin,이병환,황성희,Sang-Mok Lee,이병철,박철승,하달수,나승열 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.2

Ginsenosides, active ingredients of Panax ginseng, are known to exhibit neuroprotective effects. Large-conductance Ca^(2+)-activated K^+ (BK_(Ca)) channels are key modulators of cellular excitability of neurons and vascular smooth muscle cells. In the present study, we examined the effects of ginsenosides on rat brain BK_(Ca) (rSlo) channel activity heterologously expressed in Xenopus oocytes to elucidate the molecular mechanisms how ginsenoside regulates the BK_(Ca) channel activity. Ginsenoside Rg_3 (Rg_3) enhanced outward BK_(Ca) channel currents. The Rg_3-enhancement of outward BK_(Ca) channel currents was concentration-dependent, voltage-dependent, and reversible. The EC50 was 15.1± 3.1 μM. Rg_3 actions were not desensitized by repeated treatment. Tetraetylammonium (TEA), a K^+ channel blocker, inhibited BK_(Ca) channel currents. We examined whether extracellular TEA treatment could alter the Rg_3 action and vice versa. TEA caused a rightward shift of the Rg_3 concentration-response curve (i.e., much higher concentration of Rg_3 is required for the activation of BK_(Ca) channel compared to the absence of TEA), while Rg_3 caused a rightward shift of the TEA concentration-response curve in wild-type channels. Mutation of the extracellular TEA binding site Y360 to Y360I caused a rightward shift of the TEA concentration-response curve and almost abolished both the Rg_3 action and Rg_3-induced rightward shift of TEA concentration-response curve. These results indicate that Tyr360 residue of BK_(Ca) channel plays an important role in the Rg_3-enhancement of BK_(Ca) channel currents.

Activation of Lysophosphatidic Acid Receptor Is Coupled to Enhancement of Ca2+-Activated Potassium Channel Currents

최선혜,이병환,황성희,이상목,나승열,김현중 대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.3

The calcium-activated K+ (BKCa) channel is one of the potassium-selective ion channels that are present in the nervous and vascular systems. Ca2+ is the main regulator of BKCa channel activation. The BKCa channel contains two high affinity Ca2+ binding sites, namely, regulators of K+ conductance,RCK1 and the Ca2+ bowl. Lysophosphatidic acid (LPA, 1-radyl-2-hydroxy-sn-glycero-3-phosphate) is one of the neurolipids. LPA affects diverse cellular functions on many cell types through G proteincoupled LPA receptor subtypes. The activation of LPA receptors induces transient elevation of intracellular Ca2+ levels through diverse G proteins such as Gαq/11, Gαi, Gα12/13, and Gαs and the related signal transduction pathway. In the present study, we examined LPA effects on BKCa channel activity expressed in Xenopus oocytes, which are known to endogenously express the LPA receptor. Treatment with LPA induced a large outward current in a reversible and concentration-dependent manner. However, repeated treatment with LPA induced a rapid desensitization, and the LPA receptor antagonist Ki16425 blocked LPA action. LPA-mediated BKCa channel activation was also attenuated by the PLC inhibitor U-73122, IP3 inhibitor 2-APB, Ca2+ chelator BAPTA, or PKC inhibitor calphostin. In addition, mutations in RCK1 and RCK2 also attenuated LPA-mediated BKCa channel activation. The present study indicates that LPA-mediated activation of the BKCa channel is achieved through the PLC, IP3, Ca2+, and PKC pathway and that LPA-mediated activation of the BKCa channel could be one of the biological effects of LPA in the nervous and vascular systems.

ITIL기반 IT서비스 관리 체계 구축

최선혜 대한산업공학회 2007 ie 매거진 Vol.14 No.3

조선 전기 재지품관의 제사와 기복 민속의식

최선혜 조선시대사학회 2004 朝鮮時代史學報 Vol.29 No.-

Changes in c-Fos Expression in the Forced Swimming Test: Common and Distinct Modulation in Rat Brain by Desipramine and Citalopram

최선혜,정성,조진희,조윤하,김진욱,김정민,김희정,김현주,신경호 대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Rodents exposed to a 15-min pretest swim in the forced swimming test (FST) exhibit prolonged immobility in a subsequent 5-min test swim, and antidepressant treatment before the test swim reduces immobility. At present, neuronal circuits recruited by antidepressant before the test swim remain unclear, and also less is known about whether antidepressants with different mechanisms of action could influence neural circuits differentially. To reveal the neural circuits associated with antidepressant effect in the FST, we injected desipramine or citalopram 0.5 h, 19 h, and 23 h after the pretest swim and observed changes in c-Fos expression in rats before the test swim, namely 24h after the pretest swim. Desipramine treatment alone in the absence of pretest swim was without effect, whereas citalopram treatment alone significantly increased the number of c-Fos-like immunoreactive cells in the central nucleus of the amygdala and bed nucleus of the stria terminalis, where this pattern of increase appears to be maintained after the pretest swim. Both desipramine and citalopram treatment after the pretest swim significantly increased the number of c-Fos-like immunoreactive cells in the ventral lateral septum and ventrolateral periaqueductal gray before the test swim. These results suggest that citalopram may affect c-Fos expression in the central nucleus of the amygdala and bed nucleus of the stria terminalis distinctively and raise the possibility that upregulation of c-Fos in the ventral lateral septum and ventrolateral periaqueductal gray before the test swim may be one of the probable common mechanisms underlying antidepressant effect in the FST.

단릉(丹陵) 이윤영(李胤永)의 시문에 나타난 영남의 인상 : 1728년 무신란(戊申亂)의 흔적을 중심으로

최선혜 이화여자대학교 한국문화연구원 2014 한국문화연구 Vol.27 No.-

국문초록l1728년(영조 4) 이인좌를 중심으로 소론 강경파 및 남인 소북 세력은 경종의 복수를 명분으로 전국적인 규모의 무신란(戊申亂)을 일으켰다. 반란은 같은 소론의 오명항(吳命恒) 박문수(朴文秀) 등에게 17일만에 진압되었으나 그 여파는 막대하였다. 특히 영남은 누대의 명문세족들이 대거 몰락하였으며 사실상의 정치적 금고 상태에 놓이게 되었다. 이윤영(李胤永)은 노론의 강경파로서 평생 영조의 탕평책에 반대하여 벼슬을 포기하고 일생을 기행과 문학 창작에 바친 문인이었다. 그 중 영남 방문은 도합 세 차례 이루어진 것으로 보인다. 1740년에 금산 군수로 부임한 백부 이화중과 함께, 1748년에는 중부 이태중의 동래 부사 부임길에 동행했다. 그리고 1751년 단양 군수로 부임한 부친 이기중과 함께 간 단양에서 영남으로 유배를 떠나는 이유수를 만나 벗이 되어 안동까지 동행했다. 이윤영은 이러한 여행길에서 영남대로를 지나며 무신란과 임진왜란의 흔적 수려한 산수 명승지와 마주하였다. 교유의 범주에서 노론계 영남 문인들이 대부분이었다는 점에서 한계를 보였으나 이윤영은 영남의 전통과 무신란 이후의 현실에 대하여 안타까운 감정을 시에 담았다. 또한 세 차례에 걸쳐 영남을 방문하면서 시에 드러나는 심리는 옛 전란에 대한 불안에서 안타까움과 위로로 변화하는 양상을 보였다. 따라서 노론 문인의 영남에 대한 인식의 폭을 읽어낼 수 있다는 데 의의가 있다. The Mushin Rebellion(戊申亂) that broke out in 1728(Year 4 of Yeongjo) was one of the largest uprisings in the history of the Joseon Dynasty. It was instigated in 1728, the year of Mushin, on the pretext of revenging King Gyeongjong by an alliance of Soron hardliners and Namin(南人)/Sobuk(小北) political factions, and it was put down by Oh Myeong-hang(吳命恒) and Bak Mun-su(朴文秀), the ministers from the moderate Soron faction. Although the rebellion was suppressed in 17 days, it had a tremendous impact. Many noble families in Gyeongsangdo were ruined, the province was blamed for being the seat of the rebellion, and the families of Namin and Soron/Sobuk factions were placed in de facto political imprisonment. He has 3 times journey to yeongnam(嶺南). First, he went with uncle Yi Hwa-jung who was appointed governor in 1740. Second, he was accompanied uncle Yi Tae-jung who was appointed to the high government post of Dongrae Busa in 1748. And he see off Yi Yoo-soo who met by friend in Danyang. At this moment, his father Yi Gi-jung was appointed governor of Danyang, then he lived in Danyang. However, He defined the boundary in Noron scholars in Yeongnam. The Youngnam journey appearing in Yi Yun-yeong’s anthology Dalleungyugo(丹陵遺稿) follows the path of the Mushin Rebellion. On the road through Yeongnam there are beautiful views and countless scenic spots. Therefore throughout the poetry in Yi Yun-yeong’s anthology Dalleungyugo the writer’s complex feelings are expressed. From this we can grasp Yi Yun-yeong’s admiration for the old literary tradition of Yeongnam as well as his mixed sentiments about the region as the place of the rebellion.

Differential effects of ginsenoside metabolites on slowly activating delayed rectifier K+ and KCNQ1 K+ channel currents

최선혜,이병환,김현중,정석원,황성희,나승열 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.3

Channels formed by the co-assembly of the KCNQ1 subunit and the mink (KCNE1) subunit underline the slowly activating delayed rectifier K+ channels (IKs) in the heart. This K+ channel is one of the main pharmacological targets for the development of drugs against cardiovascular disease. Panax ginseng has been shown to exhibit beneficial cardiovascular effects. In a previous study, we showed that ginsenoside Rg3 activates human KCNQ1 K+ channel currents through interactions with the K318 and V319residues. However, little is known about the effects of ginsenoside metabolites on KCNQ1 K+ alone or the KCNQ1 + KCNE1 K+(IKs) channels. In the present study, we examined the effect of protopanaxatriol (PPT) and compound K (CK) on KCNQ1 K+ and IKs channel activity expressed in Xenopus oocytes. PPT more strongly inhibited the IKs channel currents than the currents of KCNQ1K+ alone in concentration- and voltage-dependent manners. The IC50 values on IKs and KCNQ1 alone currents for PPT were 5.18±0.13and 10.04±0.17 μM, respectively. PPT caused a leftward shift in the activation curve of IKs channel activity, but minimally affected KCNQ1 alone. CK exhibited slight inhibition on IKs and KCNQ1 alone K+ channel currents. These results indicate that ginsenoside metabolites show limited effects on IKs channel activity, depending on the structure of the ginsenoside metabolites

조선전기 지방의 산천제(山川祭)와 재지품관

최선혜 국립민속박물관 2003 민속학연구 Vol.- No.13

ITU-R SG1

최선혜,Choe, Seon-Hye 한국정보통신기술협회 2007 TTA저널 Vol.112 No.-

단릉(丹陵) 이윤영(李胤永)의 시문에 나타난 영남의 인상-1728년 무신란(戊申亂)의 흔적을 중심으로-

최선혜 이화여자대학교 한국문화연구원 2014 한국문화연구 Vol.27 No.-

The Mushin Rebellion(戊申亂) that broke out in 1728(Year 4 of Yeongjo) was one of the largest uprisings in the history of the Joseon Dynasty. It was instigated in 1728, the year of Mushin, on the pretext of revenging King Gyeongjong by an alliance of Soron hardliners and Namin(南人)/Sobuk(小北) political factions, and it was put down by Oh Myeong-hang(吳命恒) and Bak Mun-su(朴文秀), the ministers from the moderate Soron faction. Although the rebellion was suppressed in 17 days, it had a tremendous impact. Many noble families in Gyeongsangdo were ruined, the province was blamed for being the seat of the rebellion, and the families of Namin and Soron/Sobuk factions were placed in de facto political imprisonment. He has 3 times journey to yeongnam(嶺南). First, he went with uncle Yi Hwa-jung who was appointed governor in 1740. Second, he was accompanied uncle Yi Tae-jung who was appointed to the high government post of Dongrae Busa in 1748. And he see off Yi Yoo-soo who met by friend in Danyang. At this moment, his father Yi Gi-jung was appointed governor of Danyang, then he lived in Danyang. However, He defined the boundary in Noron scholars in Yeongnam. The Youngnam journey appearing in Yi Yun-yeong’s anthology Dalleungyugo(丹陵遺稿) follows the path of the Mushin Rebellion. On the road through Yeongnam there are beautiful views and countless scenic spots. Therefore throughout the poetry in Yi Yun-yeong’s anthology Dalleungyugo the writer’s complex feelings are expressed. From this we can grasp Yi Yun-yeong’s admiration for the old literary tradition of Yeongnam as well as his mixed sentiments about the region as the place of the rebellion. 1728년(영조 4) 이인좌를 중심으로 소론 강경파 및 남인‧소북 세력은 경종의 복수를 명분으로 전국적인 규모의 무신란(戊申亂)을 일으켰다. 반란은 같은 소론의 오명항(吳命恒)‧박문수(朴文秀) 등에게 17일만에 진압되었으나 그 여파는 막대하였다. 특히 영남은 누대의 명문세족들이 대거 몰락하였으며 사실상의 정치적 금고 상태에 놓이게 되었다. 이윤영(李胤永)은 노론의 강경파로서 평생 영조의 탕평책에 반대하여 벼슬을 포기하고 일생을 기행과 문학 창작에 바친 문인이었다. 그 중 영남 방문은 도합 세 차례 이루어진 것으로 보인다. 1740년에 금산 군수로 부임한 백부 이화중과 함께, 1748년에는 중부 이태중의 동래 부사 부임길에 동행했다. 그리고 1751년 단양 군수로 부임한 부친 이기중과 함께 간 단양에서 영남으로 유배를 떠나는 이유수를 만나 벗이 되어 안동까지 동행했다. 이윤영은 이러한 여행길에서 영남대로를 지나며 무신란과 임진왜란의 흔적‧수려한 산수‧명승지와 마주하였다. 교유의 범주에서 노론계 영남 문인들이 대부분이었다는 점에서 한계를 보였으나 이윤영은 영남의 전통과 무신란 이후의 현실에 대하여 안타까운 감정을 시에 담았다. 또한 세 차례에 걸쳐 영남을 방문하면서 시에 드러나는 심리는 옛 전란에 대한 불안에서 안타까움과 위로로 변화하는 양상을 보였다. 따라서 노론 문인의 영남에 대한 인식의 폭을 읽어낼 수 있다는 데 의의가 있다.