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Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604
Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3
A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.
서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)
김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3
Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.
최미혜,김경희,김귀옥,김기숙,김수강,김정신,김춘숙,노흥진,박지연,성혜연,오명선,이선희,이원옥,이윤영,이현수,장명재,차혜경,채정선,홍상희 중앙대학교 의과대학 간호학과 간호과학연구소 2001 중앙간호논문집 Vol.5 No.1
This study was designed to exam the aged's needs for spiritual nursing care. The purpose was to serve as a basis for the development of spiritual nursing practice. The major findings are as follows : 1. The degree of needs for spiritual nursing care as area was that needs of love and relationship mean 22.0, needs of meaning and object mean 28.2, needs of forgiving mean 13.5. Total needs for spiritual nursing care mean 63.7, which was on the upper middle level. The needs of meaning and object was rated highest. 2. Among the general characteristics of the subjects, needs of love and relatiohship wasn't significanlty different. 3. Among the general characteristics of the subjects, needs of meaning and object was significantly different according to two factors : age(F=7.260, p=0.001), religion(F=5.275, p=0.001). Higher needs of meaning and object was possessed by the older than the younger, by the one who have religion than the other. 4. Among the general characteristics of the subjects, four factors made a significantly difference to needs of forgiving : sex(t=-2.851, p=0.006), age(F=8.201, p=0.001), religion(F=6.928, p=0.000), disease(t=2.327, p=0.024). Higher needs of forgiving was possessed by man than woman, by the older than the younger, by the one who have religion than the other, by the one who have disease than the other.
( Kim¸ Ji Hee ) 동국대학교 영어권문화연구소 2014 영어권문화연구 Vol.7 No.1
Byron was different from most of his fellow contemporary writers who wrote about the East because his writings were not totally dependent on his imagination or written texts. He experienced Eastern culture first hand and had several different perspectives of the East. However, like other writers, Byron was still unable to free himself from Western point of views. This paper explores Byron's fascination with the East and the limitation of his perspective. The Seraglio in Don Juan was portrayed in detail as a magnificent and luxurious place. He simultaneously described the people who Juan met in the Seraglio. Byron also located a man in the harem as a slave and took everything Juan had. Juan, having been subordinated, was in an unusual position for a European. However, he could not go beyond the bounds of European convention in Don Juan. The description of Eastern places implies an awareness of superiority of Western culture. It seems to focus on material abundance although the palace was portrayed in detail. Byron described Oriental characters as negative and simple. There is an enlightenment mechanism to one of the characters in Don Juan, Gulbeyaz. Even though Byron had Eurocentric values, he clearly possessed a more positive attitude toward the East. His ambivalent attitude toward the East would be a great topic to further explore in researching Orientalism.
An affair of the heart: Sensibility, Revolution, Gender in Letters Written in France
( Kim¸ Ji Hee ) 동국대학교 영어권문화연구소 2014 영어권문화연구 Vol.7 No.2
Helen Maria Williams, one of the early romantic women writers, was a controversial figure of her age on the account of her being pro-French Revolution. Her support for the French Revolution is well expressed in Letters from France which were published in eight volumes between 1790 and 1796. Among them, I will focus on the first of volume of the Letters. This paper will explore why Williams used sensibility in her Letters (1790) and how she shows the sensibility with the political point of views in the epistolary form and the Memoir of Mos. and Madam Duf ―. Sensibility in Letters seems to be one of the ways to avoid critical remarks about female political prose. Williams would also use sensibility in Letters to accept her idea easily. In addition, the epistolary form assists Williams in expressing her political point of views with sensibility well in Letters and allows her to express her feelings freely and frankly. She can effectively persuade readers to sympathise with her feelings in the epistolary form. The other strategy enables Williams to show her sensibility with the political standpoint is the Memoir of Mons And Madam Duf - in later chapters of the Letters. The memoir is seemingly the love story but there is the contrast between the despotic government and the new government and the process of the revolution behind the romantic story. It is a sentimental story for covering Williams' political voice well. Williams's sentimental strategy looked to be a huge success before the revolution became violent. She expressed her political points of view with sensibility which could be the bulwark of her defence.
김지열,송호천,양광희,최근희,채기문,범희승,김광윤 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.3
본 연구에서는 마우스에 이미 오염된 방사성스트론튬(Sr-85)을 제거하는데 수용성카이토산이 어느정도 효과가 있는지 알아보고자 하였다. Sr-85를 정맥주사한 경우 주사후 1일째의 체내 잔류량은 72.9 ±5.7%, 5일째는 57.7 ±1.9%, 7일째는 54.2 ±1.4%로 서서히 감소하였으며, Sr-85를 복강내 주사한 경우는 주사후 5일째 54.4 ±1.2%, 15일째 50.6 ±0.8%로 정맥주사에 비해 낮은 잔류량을 보였다(5일째 잔류량의 비교, P<0.05), 0.3% 수용성카이토산을 1회 정맥주사해준 제21군 및 10% 수용성카이토산을 식이중에 섞어 먹인 제5군은 각각의 대조군에 비해 체내 잔류방사능의 차이가 없었으나 (P>0.05), 0.3% 수용성카이토산을 3일간 정맥주사한 제3군과 3% 수용성카이토산을 이틀 간격으로 15일간 복강내 주사한 제6군에서는 각각의 대조군에 비해 낮은 잔류방사능을 보였다. (P<0.01). 결론적으로 수용성카이토산을 연속적으로 정맥투여하거나 복강내 투여하는 경우에는 스트론튬의 골대사촉진 또는 골중의 스트론튬과의 반응등을 통해 그 배출을 촉진시킬 것으로 사료되었다. The aim of the present study was to elucidate the effect of the water soluble chitosans on the removal of contaminated radiostrontium(Sr-85) from the bone of mice. The remaining radioactivities in intravenously injected controls(group 1) were higher than in intraperitoneally injected controls (group 4, P<0.01). The % retention at day 5 were 57.7 ±1.9%, 54.4 ±1.2%, respectively. Single intravenous injection of 0.3% water soluble chitosan and continuous oral ingestion of 10% water soluble chitosan for 15 days were ineffective on the removal of contaminated radiostrontiums. Multiple intravenous or intraperitoneal injections of water soluble chitosan effectively removed contaminated radiostrontiums (P<0.01 vs controls). In conclusion, water soluble chitosan might remove once incorporated radiostrontium from bones of mice. further studies were needed to elucidate the mechanism of the removal.
대학생의 취업스트레스와 자아존중감, 자아효능감, 외모관리행동의 관계
김보라,김유진,김지희,노혜진,오승희,홍지혜,황윤희,박선아 水原大學校 2016 論文集 Vol.30 No.-
Purpose: Many university students are under a lot of job-seeking stress because the employment rate is low. This study sought to determine the relationship between job-seeking stress, self esteem, self efficacy and appearance management behavior amongst university students focusing on nursing. Methods: This descriptive correlational study evaluated the relationships between job-seeking stress, self esteem, self efficacy and appearance management behavior of 340 university students was conducted between September 1 and October 5, 2015. Results: The correlational analysis between job-seeking stress, self esteem, self efficacy and appearance management behavior revealed a negative correlation between job-seeking stress, self esteem and self efficacy and a positive correlation between job-seeking stress and appearance management behavior. In addition, age also correlated with self esteem, self efficacy and appearance management behavior. Conclusion: Stress correlated with self esteem and self efficacy and appearance management behavior. Therefore job-seeking stress may be reduced through not only self esteem and self efficacy enhancement programs but also management according to age.