Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions

Son, Hyun-Jin,Chu, Jung-Youb,Cho, Eui-Sic,Lee, Dong-Geun,Min, Myung-Gee,Lee, Suk-Keun,Cho, Nam-Pyo The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Expression of invasion/metastasis suppressor, E-cadherin, is reduced in many types of human carcinomas. Although somatic and germline utations in the CDH1, which encodes the human E-cadherin, have frequently been reported in cases with diffuse gastric and lobular breast ancers, irreversible genetic inactivations are rare in other human carcinomas. Recently, it has been well documented that some genes in human cancers may be inactivated by altered CpG methylation. Herein, we determined the expression and methylation status of E-cadherin in oral squamous cell carcinoma (SCC) by immunohistochemistry and methylation-specific PCR. The expression of E-cadherin was significantly higher in the well-differentiated oral SCCs than the moderately or poorly differentiated ones. None of eight tested benign pithelial hyperplasias showed aberrant methylation, whereas five of 12 oral squamous cell carcinomas showed aberrant methylation. When we compared E-cadherin expression with methylation status, oral SCCs with normal methylation showed a higher expression of E-cadherin than those with methylation. These findings suggest that aberrant CpG methylation of CDH1 promoter region is closely associated with transcriptional inactivation and might be involved in tumor progression of the oral mucosa.

골분을 이용한 구리 흡착 첨착

김은정,박정훈,김정섭,유현철,이봉섭,곽명화,우성훈,박승조 東亞大學校 環境問題硏究所 2005 硏究報告 Vol.27 No.1

Spent pig bone was consist of hydroxyapatite. And then we thought that it have a possibility as a adsorbent. The preparation of bone char prepared from spent pig bone in this study. We study on the recycling possibility as a adsorbent is able to adsorb copper ion from their single aqueous solutions. The results are summarized as follows. Bone char that prepared from spent pig bone at 105, 400, 700℃. The adsorption removal efficiency of copper ion onto bone char as adsorbent was above 90% for 15 hours. Increase in the initial pH of the copper ion solution result in an increase in the copper ion uptake per unit weight of the adsorbent. Freundlich isotherm model was found to be applicable for the experiment data of copper ion. The results showed that spent pig bone can be used for the absorption of the copper ion.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.

Three-Dimensional Porous Collagen/Chitosan Complex Sponge for Tissue Engineering

Kim, Sung Eun,Cho, Yong Woo,Kang, Eun Jung,Kwon, Ick Chan,Lee, Eunhee Bae,Kim, Jung Hyun,Chung, Hesson,Jeong, Seo Young The Korean Fiber Society 2001 Fibers and polymers Vol.2 No.2

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its boilogical stability and enhance its mechanical properties. The crosslinking medium has a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

위암 환자의 복강내 투여를 위한 Activated Charcoal-Alginate Bead 제형으로부터 Mitomycin C의 용출 거동

이진호,최선웅,서중기,김동민,정경수,오정연,김진향,노승무,민병무,김용백,김창식,박근성,강대영,송규상,양준묵,조준식,정현용,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherapy seems a logical choice for cancer chemotherapy. In this study, Mitomycin C (MMC)-activated charcoal (CH)-alginate (ALG) beads were prepared by the mixtures of CH particles adsorbed with MMC as an anti-cancer drug and aqueous alginate solution. The alginate is recognized as biodegradable, nontoxic, and biocompatible. The release of MMC from the beads in 0.1 M Tris buffer was stable and continuous until about 1 week. The MMC-CH-ALG beads can be applied in the peritoneal cavity for intraperitoneal chemotherapy since they provide a good adhesiveness on the tissue and controlled release pattern of the drugs.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

밭토양에 처리된 규산질 비료의 유효화와 토양반응 교정효과

조현종,최희열,이용우,이윤정,정종배 한국환경농학회 2004 한국환경농학회지 Vol.23 No.2

이화학적 특성이 다른 4가지의 밭토양에 대한 규산질 비료를 처리하여 토양의 유효규산 함량과 pH 변화에 미치는 효과를 조사하였다. 토양의 이화학적 특성과 기존 유효규산 함량에 상관없이 토양 유효규산 함량은 규산질 비료 시용량에 비례하여 증가 하였다. 규산질 비료의 유효화 정도는 토양마다 차이가 있었으며 최저 9.1%에서 최고 19.2% 정도의 비율을 나타냈다. 규산질 비료 100 kg/10a 수준의 처리는 용탈 등으로 제거되지 않는 한 10 cm 깊이 표토에 평균적으로 100 mg/kg 정도 의 유효규산을 공급할 수 있는 것으로 나타났다. 퇴비 혼합처리는 토양 유효규산 함량 증가에 큰 영향을 미치지 못했으며, 석회 처리는 유효규산 함량을 다소 증가시켰다. 토양 pH 변화는 모든 토양에서 규산질 비료 시비량에 비례하여 증가하였으며, 규산질 비교 100 kg/10a 처리로 토양 pH를 0.1~0.2 단위 증가시킬 수 있는 것으로 나타났다. 따라서 산성 토양의 경우에는 규산질 비료의 시용과는 별도로 pH 교정을 위해 석회비료를 시용해야 할 것이다. Although silicon (Si) has been known to be an essential element for rice growth, the optimum soil level of Si for upland crops remains unestablished. This study was conducted to estimate the availability of Si fertilizer in upland soils, and also effect of the Si fertilizer on soil pH was examined. Different application rates of Si fertilizer were tested using four soils of different available Si levels and pHs in a series of laboratory incubation study. The treatments included Si fertilizer levels of 100, 200, and 300 kg/10a. Also to examine the effects of compost and lime on the availability of Si fertilizer in upland soil, treatment of silicate fertilizer 200 kg/10a + compost 1,000 kg/10a and lime alone treatment were included. Changes of Si availability in the soils during the incubation period were measured by 1 N NaOAc extraction procedure. Availability of Si fertilizer was different among the tested soils, and about 9.1~19.2% of the applied Si fertilizer was extracted after 60 days laboratory incubation. Application rate could not influence the availability of Si fertilizer. Application of compost with Si fertilizer could not increase Si availability in upland soils, but lime treatment could increase Si availability. Soil pH increased by application of Si fertilizer, but the effect of Si fertilizer on soil pH was minimal. When Si fertilizer is applied on the purpose of Si nutrition in acid upland soils, lime treatment should be coupled with the Si fertilizer for remediation of soil acidity.

수림가장자리의 경관생태적 특성분석

조현주,나정화 慶北大學校農業科學技術硏究所 2007 慶北大農學誌 Vol.25 No.-

The aim of this research is to set improvement guidance a character analysis of woodland edge to cope with the ecological dysfunction of woodland which was caused by massive development project and thoughtless development in country areas. The summary of research result are as follows. 1) From the result of landscape ecology characteristic analysis of woodland in all seven research sites, to begin with, in proportion of appearance by vegetation layer and condition of composition, site 5 showed to be most satisfactory. 2) A width of woodland edge was revealed 7.5m as a minimum, 17.0m as a maximum, and 11.4m as a average and minimum edge was set as 10m according to integrated analysis on each example place. 3) As a result of flexibility analysis, site 1, 2 and 5 was shown high value 3, and it is thought that curve rather than linearity should be maintained in order to increase the ecological function. Also, a phenomenon of straight was prominent, and as a woodland edge, green network and buffering system showed to be somewhat unsatisfactory. 4) Based on the result of character analysis of landscape ecology, main guidelines for improvement of woodland edge were categorized into five in parallel structure and three in vertical structure respectively. The guidelines for improvement of woodland edge suggested by the research has a deep meaning in that it is used as a basic material to induce for controling more systematically or landscape-friendly the defamed forest problems caused by road construction, various development projects, and enlargement of agricultural lands.

EPC class-1 generation-2 표준의 RFID 태그의 RF/아날로그 설계

조정현,김학수,김시호 충북대학교 컴퓨터정보통신 연구소 2006 컴퓨터정보통신연구 Vol.14 No.2

RFID는 사용되는 주파수에 따라 ID 확인과 실별, 물류, 전자지불, 출입통제, 교통카드 등에 사용되고 있다. 그중 UHF대역의 RFID 시스템은 유비쿼터스의 대표기술로 인식되고 있으며, 바코드를 대체하는 기술로 그 응용분야가 급속히 증가할 것으로 기대되고 있다. 본 논문은 EPCglobal Class-1 Generation-2 표준을 만족하는 UHF RFID 태그의 구성과 RF/아날로그 전단부 회로 설계를 설계하였다. 본회로는 매그너 칩의 0.25 CMOS 공정 파라미터를 사용하여 설계하였다. RFID systems can identify, trace and manage the objects equipped with a semiconductor tag chip. Tag chip stores and extracts the electronic code in the tag memory through RF signals. A typical RFID system consists of a reader and a number of tags. This paper describes the design of analog circuit of a RFID tag that is compatible with the EPCglobal Class-1 Generation-2 Protocol.