人蔘에탄올엑기스가 鉛毒性에 미치는 影響

안영근,정종갑,김주영,김정훈,김관수 한국환경독성학회 1987 환경독성보건학회지 Vol.2 No.1

The effects of ginseng ethanol extract on the toxicity of lead acetate in mice were examined. Mice were given intraperitoneally daily doses of lead acetate 50mg/kg with ginseng ethanol extract 50mg/kg, 100mg and 200mg/kg for 3 weeks. The exposure of lead acetate showed the toxicity at all experimental assay such as the gain of body weight, the ratio of some organs weight to body weight, serum transaminase activity and creatinine value, hematocrit and WBC counts. These toxicities were inhibited significantly by the ginseng ethanol extract administration. The 50mg/kg and 100mg/kg administration of ginseng ethanol extract inhibited histopathological changes on kidney by lead acetate, whereas the 200mg/kg administration of the fraction enhanced histopathological changes.

Vitamin A가 마우스의 免疫反應에 미치는 影響

安榮根,金周永,金正勳 원광대학교 식품약품안전성연구소 1987 食品藥品安全性硏究 Vol.1 No.-

The effect of vitamin A on the immune response in ICR mice was studied. The effects were evaluated by immuno organ weight, peripheral circulating white blood cells, HA and HY titer, peritoneal exudate cells, RFC, Arthus reaction and DTH in mice. The spleen of mice was significantly hypertrophied by deficiency or over doses of vitamin A as compared with control group (50IU/㎏). Arthus reaction, RFC and peritoneal exudate cells were sharply decreased according to the increase of vitamin A doses. The number of white blood cell was decreased according to the increase of vitamin A doses, but in the case of vitamin A 50,000 IU/㎏ treated group, it was significantly increased. These results suggest that deficiency or over dose of vitamin A decrease ed humoral and cellular immune response.

랏트에 있어서 에탄올과 삭카린이 免疫反應에 미치는 影響

안영근,김주영,김정훈,염정열 원광대학교 식품약품안전성연구소 1987 食品藥品安全性硏究 Vol.1 No.-

Experiments were performed to investigate effects of ethanol and saccharin on the immune system in rats. 4% ethanol and 0.02, 0.20, 2.00% saccharin solution in 4% ethanol were provided ad libitum by tap water for 4 weeks. Rats were sensitized and challenged with sheep red blood cells(S-RBC). Immune responses were evaluated by relative immuno organ weight, antibody production, Arthus reaction, delayed type hypersensitivity, and rosette forming cell. Ethanol exposure decreased thymus weight and delayed type hypersensitivity. A combined solution of ethanol and saccharin decreased water intake, growth rate, spleen weight, thymus weight, humoral and cellular immune response. Especially, a 2% saccharin solution in 4% ethanol very significantly suppressed cellular immunity.

마우스에 있어서 Cimetidine, Ranitidine 및 Famotidine이 면역반응에 미치는 영향

안영근,김정훈,이상근 환경독성보건학회 1990 환경독성보건학회지 Vol.5 No.3

Experiments were performed on mice to investigate the influences of cimetidine, ranitidine and famotidine on the immune response. Immune response were evaluated by antibody, Arthus reaction (Arthus), delayed type hypersensitivity (DTH), rosette forming cell (RFC), phagocyte activity and whit( blood cell (WBC) in mice, sensitized and challenged with sheep red blood cells (SRBC). The weight of liver, spleen and thymus were measured. Following results obtained in this experiment. 1) The administration of cimetidine as compared to normal group significantly decreased Arthus, Hemagglutinin titer (HA), RFC, DTH, WBC and phagocyte activity, but increased the activity of serum albumin. 2) The administration of ranitidine as compared to normal group decreased RFC and HA. 3) The administration of Famotidine as compared to normal group decreased DTH and RFC, and significantly decreased HA, Arthus and serum protein. 4) The administration of ranitidine and famotidine decreased more humoral immune response than cellular immune response, but the administration of cimetidine significantly decreased humoral and cellular immune response, WBC and phagocyte activity.

클로람페니콜의 免疫毒性에 미치는 食餌脂肪의 影響

安榮根,金周永 원광대학교 식품약품안전성연구소 1987 食品藥品安全性硏究 Vol.1 No.-

chloramphenicol은 靜菌 및 殺菌作用을 갖은 抗生物質로서 骨髓의 毒性에 의하여 再生不良性貧血, 血液異混和症 및 汎血球減少症을 일으키며 이는 直接的인 毒作用에 의하거나 人體內에서 hapten으로 作用하여 自家免疫反應을 誘發함에 기인하며^1∼3), 免疫形成에도 약간의 影響을 미친다고 報告되어 있다.^4∼7) 한편 不飽和脂肪酸은 prostaglandin E_1 및 E_2의 合成을 增加시키어 淋巴球의 免疫機能을 抑制하며, 설치류의 皮膚同種移植生存率을 延長시키고 腎臟移植時 免疫抑制劑의 補助劑로 使用할 수 있으며, 淋巴球幼若化反應을 in vitro에서 抑制한다.^8∼11) Weyman 등^12)은 phytohemagglutinin(이하 PHA)에 의한 淋巴球의 uridine incorporation이 胞和 및 不胞和脂肪酸에 의해서 抑制되나 胞和 및 不胞和脂肪酸의 적절한 混合에 의하여 그 抑制作用을 減少시킬 수 있다고 하였으며, Kollmorgen 등^13)은 高脂肪食餌는 mitogen의 作用을 抑制한다고 하였으며, Fernandes 등^14∼15) 및 Ibrahim 등^16)은 高脂肪食餌는 自家免疫疾患을 顯著하게 發顯시킨다고 하였다. 따라서 著者는 클로람페니콜의 免疫毒性을 究明함과 아울러, 이러한 毒性이 食餌脂肪의 種類 및 量에 의하여 影響을 받을 것으로 예측되어 近來 食生活에서 섭취가 增加하고 있는 쇠기름(포화지방) 및 들기름(불포화지방)을 各種 組成으로 給與하여 마우스를 飼育하여 클로람페니콜을 投與한 후 細胞性 및 體液性免疫反應을 緬羊赤血球 抗原을 이용, 檢査하여 有意한 結果를 얻었기에 報告하는 바이다. The effect of dietary fat on the immunotoxicity of chloramphenicol was investigated in mice, sensitized and challenged with sheep red blood cells. Chloramphenicol suppressed more cell-mediated immunity than humoral immunity. The saturated fat diet elevated humoral and cell-mediated immunotoxicity, whereas the unsaturated fat diet decreased humoral immunotoxicity, but elevated cell-mediated immunotoxicity of chloramphenicol. Especially, the normal diet in saturated and unsaturated fat restored immunosuppressive effect of chloramphenicol compared to the saturated fat diet and the unsaturated fat diet.

Amygdalin의 經口投與毒性에 미치는 Cysteine과 Streptomycin의 影響

安榮根,吳琮,鄭址乾,金正勳 한국환경독성학회 1989 환경독성보건학회지 Vol.4 No.2

Amygdalin has been used for a long time as an anticancer agent. But because of its toxicity, it is difficult to administer continuously for treatment of cancer. This paper was attempted to reduce the side effect and toxicity of amygdalin. That is, effects of cysteine and streptomycin on the toxicity of amygdalin were investigated in rats orally administered amygdalin. 1. The group administered only amygdalin 400 mg/kg was effected on the lung and body weight, hematocrit, hemoglobin, clotting time, SGOT and albumin value. That is, lung and body weight, hematocrit hemoglobin and albumin value were significantly decreased. SGOT and clotting time were significantly increased compared with those of normal group. 2. Weight of lung was significantly increased in the C group (administred amygdalin 400 mg/kg and cysteine 300 mg/kg), D group (amygdalin 400 mg/kg and streptomycin 10 mg/kg), E group (amygdalin 400 mg/kg, streptomycin 10 mg/kg and cysteine 200 mg/kg)and F group (amygdalin 400 mg/kg, streptomycin 10 mg/kg and cysteine 300 mg/kg). 3. Values of hematocrit and hemoglobin were significantly increased, and clotting time was significantly decreased, in the E group and F group compared with those of A group. 4. SGOT was significantly decreased in the C group, E group and F group compared with that of A group. 5. The blood cyanide concentration was significantly decreased in the E group and F group compared with that fo A group. 6. In short, coadministration of cysteine and streptomycin are considered to reduce the toxicity of amygdalin in rats orally administered.

랏트에 있어서 에탄올과 식카린이 免疫反應에 미치는 影響

안영근,김주영,김정훈,염정열 한국환경독성학회 1987 환경독성보건학회지 Vol.2 No.1

Experiments were performed to investigate effects of ethanol and saccharin on the immune system in rats. 4% ethanol and 0.02, 0.20, 2.00% saccharin solution in 4% ethanol were provided ad libitum by tap water for 4 weeks. Rats were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by relative immuno organ weight, antibody production, Arthus reaction, delayed type hypersensitivity, and rosette forming cell. Ethanol exposure decreased thymus weight and delayed type hypersensitivity. A combined solution of ethanol and saccharin decreased water intake, growth rate, spleen weight, thymus weight, humoral and cellular immune response. Especially, a 2% saccharin solution in 4% ethanol very significantly suppressed cellular immunity.

마우스에 있어서 Cimetidine의 免疫毒性에 미치는 人蔘抽出物의 影響

안영근,이상근 원광대학교 식품약품안전성연구소 1991 食品藥品安全性硏究 Vol.4 No.-

The immunopotenciating effects of petroleum ether extract, ethanol extract and butanol fraction of panax ginseng on the immunotoxicity of Cimetidine were investigated in ICR mice. Immune responses were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), and rosette forming cell(RFC) in mice, sensitized and challenged with sheep red blood cells. To investigate the change of the non-specific immune responses, phagocyte activity and number of leukocytes in peripheral blood were measured also. The results of this study are summarized as followings; 1. Cimetidine treated group as compared with normal group generally decreased HA, 2-MER, RFC, number of circulating leukocytes and phagocyte activity whereas increased Arthus reaction and DTH. 2. The panax ginseng petroleum ether extract combined administration group as compared with the control group remarkably increased HA, 2-MER, number of circulating leukicytes and phagocyte activity. 3. The panax ginseng ethanol extract combined administration group as compared with the control group remarkably increased Arthus reaction, DTH, HA, RFC, number of circulating leukocytes and phagocyte activity. 4. The panax ginseng butanol fraction combined administration group as compared with the control group remarkably increased Arthus reaction, HA, 2-MER, RFC number of circulating leukocytes and phagocyte activity.

월견초종자유가 생쥐의 면역반응에 미치는 영향

안영근,오연준,김정훈 원광대학교 식품약품안전성연구소 1992 食品藥品安全性硏究 Vol.5 No.-

The purpose of this experiment was to investigate both the immunomodulatory effect of evening primrose(EP) oil and the effects of EP oil immunoregulation by cyclophosphamede in mice. EP oil at doses of 0.1, 0.2 and 0.4㎖/㎏ were orally administered to ICR male mice once daily for 28 consecutive days. Cyclophosphamide was injected intraperitoneally to ICR mice with a single dose of 5㎖/㎏ at 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells(S-RBC). Immnune responses were evaluated by humoral and cellular immune responses and non-specific immune response. The results of this study were summarzed as follows; (1) The humoral immune responses such as hemagglutination titer(HA). hemolysin titer(HY), Arthus reaction and plaque forming cell(PFC) were significantly enhanced in the low dose EP oil administered groups(0.1 and 0.2㎖/㎏). However, in the high dose EP oil administered group(0.4㎖/㎏) the responses were significantly lowered. (2) In the case of cellular immune responses, delayed type hypersensitivity reaction(DTH) was significantly decreased in EP oil whereas rosette forming cell(RFC) was remarkably enhanced. (3) Activities of natural killer cells and phagocyte were generally enhanced in EP oil. In addition, serum albumin and globulin were also increased.

環境汚染과 免疫 : 環境化學物質의 免疫毒性

安榮根 원광대학교 식품약품안전성연구소 1987 食品藥品安全性硏究 Vol.1 No.-