혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

전국 10개 종합병원 중환자실 환자의 직장내 Vancomycin-resistant Enterococci(VRE) 보균율, 보균의 위험요인 및 유전적 다양성 조사

정희진,송준영,엄중식,김우주,최선주,최정현,이혁,정문현,이광미,신동현,손보라,최종성,우흥정,권정아,이경원,최강원,박승철 대한감염학회 2002 감염 Vol.34 No.5

전유전체 서열 분석과 가시화를 위한 워크벤치 개발과 응용

최정현,진희정,김철민,장철훈,조환규 한국유전학회 2002 Genes & Genomics Vol.24 No.2

An increasing number of genome sequencing projects results in explosive growth of whole genome sequences. Furthermore the number of studies on the functions of individual genes has also been rapidly increased. However on-memory algorithms are not applicable to the analysis of whole genome sequences, since the size of individual whole gene a ranges from several million base pairs to hundreds billion base pairs. In order to effectively manipulate the huge sequence data, it is necessary to use the indexed data structure for external memory. In this paper, we introduce the development and application of the workbench for the analysis and visualization of whole genome sequences using string B-tree that is suitable for the analysis of huge data. This system consists of two main parts, the analysis query part and the visualization part. The query system supports various transactions such as pattern matching, k-occurrence, and k-mer analysis. The visualization system helps biologists to easily understand whole genome structure and specificity by various kinds of visualization such as whole genome sequence viewer, annotation viewer, CGR (Chaos Game Representation) viewer, k-mer viewer, RWP (Random Walk Plot) viewer, and map viewer. We can find the relationships among organisms, support gene prediction in a genome, and study the function of junk DNA using our workbench. In this paper, we apply our workbench to investigating specific sequence such as avoided sequence, common sequence, and classifiable sequence.

어머니의 사회화통제유형과 아동의 자아개념

정현희,최경순 동의대학교 생활과학연구소 2000 生活科學論集 Vol.4 No.-

This study was to investigate the following research questions. (1) Are there any gender differences in the mother's socialization-patterns and child's self-concept? (2) Is there any relationship between mother's socialization-patterns and child's self-concept? (3) Is child's self-concept influenced by the mother's socialization-patterns and child's gender? The subjects of this study were 235 boys and 207 girls attending elementary schools in Busan. Lee, Kyung-Hee's questionnaires(1993) on mother's socialization patterns and Kim, Ki-Jung's questionnaires(1984) on child's academic self-concept and Kim, Hee-Kyung's questionnaires(1990) on child's physical, home, social self-concept were used. The results were as follows: 1)The mother's imperative mode for boys differed significantly from that for girls. Boys perceived mother's socialization-patterns as more imperative. And boy's physical self-concept and academic self-concept differed significantly from girl's. Boy had higher scores than girl in physical self-concept and academic self-concept. 2)There were significantly correlations between mother's position-oriented mode and boy's self-concept, between mother's poison-oriented mode and boy's self-concept. 3)The mother's poison-oriented mode was the predictor variable influencing on child's academic self-concept. And the mother's position-oriented mode and the mother's person-oriented mode were the predictor variables influencing on child's home self-concept.

초등과학영재학급 학생의 학습양식과 과학탐구능력 간의 상관관계

최선영,송현정,강호감 한국초등과학교육학회 2005 초등과학교육 Vol.24 No.2

The purpose of this study was to investigate the relation between the learning styles and science process skills of students of the gifted class in elementary school. Subjects were forty-eight students of the gifted class who are in the fifth grade studying at the gifted class of S elementary school in Bucheon, M and Y elementary school in Incheon on learning styles and science process skills of students. Learning Style Profile (LSP) was used as instrument to survey learning style of students of the gifted class which was developed by NASSP, and consists of four categories (cognitive skills, perceptual response, orientation and learning preferences) and twenty-four subscales. The results of this study were as follows: 1. In the learning styles test, students of the gifted class have higher scores of spatial skill, sequential processing skill, persistence orientation, manipulative preference, temperature preference and after-noon preference than general class students, but they have lower scores of discrimination skill and lighting preference, and there were statistically significant difference. 2. In science process skills test, there were statistically significant difference between students of the gifted class and general students. 3. In the correlation between the learning styles and science process skills, there was positive correlation of observing skill with spatial skill and manipulate skill of cognitive skill domain. For classifying skill, there was positive correlation with visual perceptual response, but was negative correlations with auditory and emotive perceptual response of perceptual response domain and with evening preference and verbal risk orientation of study preference domain. For measuring skill, there was positive correlation with sequential processing skill of cognitive skill domain. For formulating hypotheses, there was positive correlation with persistence orientation of study preference domain. For controlling variables, there was positive correlation with sequential processing skill and simultaneous processing skill of cognitive skill domain, and with verbal-spatial preference and early morning study preference of study preference domain. When planning and man-aging the gifted class, it will be beneficial and effective to consider the meaningful relations between the elements of learning style and science process skills in order to improve science process skills.

한국인 제2형 당뇨병 환자의 골격근에서 인슐린 신호전달체계의 결함

최준혁,이관우,김효정,이동훈,이종우,김정은,엄현채,김경미,최성이,정윤석,김현만 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.5

연구배경: 제2형 당뇨병에서 나타나는 인슐린 저항성은 간, 지방, 근육 같은 말초조직에서 인슐린의 작용이 떨어지는 것을 말한다. 제2형 당뇨병의 발생기전에 인슐린 저항성과 인슐린 분비능 저하가 같이 관여함은 잘 알려진 사실이다. 인슐린의 세포내에서의 저항성을 규명하기 위한 인슐린의 세포내 신호전달체계에 대한 연구가 활발히 진행되고 있으며 몇몇 연구에서 인슐린 저항성을 가진 골격근육에서 IRS와 관련된 PI3-kinase의 활성감소와 Akt kinase의 활성감소를 보고하고 있으나 아직까지 명확하게 그 기작이 설명되어지지 않고 있다. 본 연구에서는 정상성인, 제2형 당뇨병 환자를 대상으로 경구당부하검사 및 인슐린 클램프검사를 시행하고 인슐린 클램프 검사시 대상인의 근육을 채취하여 인슐린 신호전달 체계(IR-β, IRS, Akt(PKB, Rac) kinase, GSK-3)를 연구하였다. 방법: 연구대상자는 한국인으로서 경구당부하검사상 정상인 및 당뇨병 환자 각각 11명, 9명을 대상으로 하였으며, 정상인은 건강인으로 과거력상 당대사에 영향을 줄 질환이 없고, 현재 당대사에 영향을 줄 약물 복용 및 다른 소견이 없는 경구당부하검사상 정성 내당능을 보이는 대상자로 하였다. 당뇨병환자는 모두 제2형 당뇨병 환자로 이환기간이 만 5년 이내인 경우로 하고, 인슐린으로 치료하는 대상자는 제외하도록 하였다. 대상자의 연령, 체질량지수, 체지방량, 공복시 총콜레스테롤, 중성지방, 고밀도지단백 콜레스테롤 및 HbA1c, 인슐린, C-peptide를 측정하였고, "HOMA model"을 이용하여 베타세포의 기능와 인슐린저항성 정도를 평가하였다. 정상혈당클램프 검사(euhlycemic hyperinsulinemic clamp test)를 시행하고 인슐린 투입 전과 인슐린 투입 30분후, 두차례에 걸쳐 대퇴부 근육생검을 실시한후 western 법으로 IR-β, IRS, Akt kinase, GSK-3의 인산화량을 측정하였다. 결과: 정상인 11명과 당뇨병 환자 9명을 대상으로 하였으며 대상자의 평균 연령, 평균 체질량지수, 체지방량, 허리/엉덩이 둘레의 비(waist hip ratio; WHR)는 두 군간에 차이가 없었다. 평균 공복혈당은 정상군 98.0±0.3㎎/dL, 당뇨병군 208.1±16.5㎎/dL(p<0.05), HbA1c는 정상군 5.4±0.5%, 당뇨병군 9.2±0.6%(p<0.05)였다. "HOMA model"을 이용한 베타세포의 기능과 인슐린저항성은 정상군 56.4±8.5%, 1.4±0.2, 당뇨병군 72.2±52.3%(p<0.01), 10.2±6.3(p<0.01)였으며 정상혈당클램프 검사상 포도당 이용률은 정상군 8.2±0.6㎎/㎏/min, 당뇨병군 3.7±1.1㎎/㎏/min(p<0.01)로 정상군과 당뇨병군간에 유의한 차이를 나타내었다. Western blot법으로 IR-β, IRS, Akt kinase, GSK-3의 인산화량을 인슐린 투입전과 인슐린 투입 30분후 측정하였을 때 IR-β에서는 정상군이 103.9±2.3에서 241.3±18.6, 당뇨군이 108.9±2.2에서 198.7±6.3으로 증가하였다(p=NS). 이는 인슐린 투입후 당뇨병군이 정상군과 비교하여 인산화량의 증가정도가 통계적인 차이가 없었다. IRS에서는 정상군이 111.6±7.3에서 295.6±17.2, 당뇨군이 114.5±6.1에서 222.0±23.2로 증가하였으며(p<0.05), 인슐린 투입후 당뇨병군이 정상군과 비교하여 인산화량의 증가정도가 약 24% 감소하였다. Akt kinase에서는 103.4±6.0에서 416.8±29.5로 증가하였으며(p<0.01), 인슐린 투입후 당뇨병군이 정상군과 비교하여 인산화량의 증가정도가 약 43% 감소하였다. GSK-3에서는 정상군이 107.7±6.7에서 595.7±28.1, 당뇨군이 104.3±4.8에서 443.3±12.9로 증가하였으며(p<0.01), 인슐린 투입후 당뇨병군이 정상군과 비교하여 인산화량의 증가정도가 약 25% 감소하였다. 결론: 제2형 당뇨병 환자에서 정상인과 비교하여 인슐린 자극후 IR-β는 인산화량의 증가정도가 큰 차이가 없었으나, IRS, Akt kinase, GSK-3에서는 인산화량의 증가 정도가 감소되는 것으로 보아 상위 인슐린 신호전단체계부터 결함이 있는 것으로 생각된다. Background: The glucose uptake rate is the limiting step in glucose utilization and storage. The failure of insulin to stimulate glucose uptake in muscle appears to be a primary defect of insulin resistance. This study was undertaken to examine the effect of physiological hyperinsulinemia on the phosphorylation of the insulin receptor (IR-β), insulin receptor substrate (IRS), Akt kinase and GSK-3 in isolated skeletal muscle, in people with type 2 diabetes(n=9) and control subjects(n=11). Methods: 75g OGTT and euglycemic hyperinsulinemic clamp test were done. And vastus lateralis muscle was obtained before and 30 min into the euglycemic clamp. Western blots were performed for tyrosine phosphroylation of insulin receptor substrate (IRS) and phosphorylation of the insulin receptor(IR-β), Akt and GSK-3. Result: There were no statistical differences in the mean age, BMI and body fat between the control subjects and diabetic patients. The fasting blood sugar and HbA_1c in controls and diabetic patients were 98.0±0.3 and 208.1±16.5ng/dl, and 5.4±0.5 and 9.2±0.6%, and 1.4±0.2 in the control subjects, and 72.2±52.3%(p<0.01) and 10.2±6.3(p<0.01) in the diabetic patients, respectively. The insulin resistance from the euglycemic hyperinsulinemic clamp test were 8.2±0.6㎎/㎏/min and 3.7±1.1ng/㎏/min in the control subjects and in the diabetic patients, respectively(p<0.01). Compared with the normal controls, insulin-stimulated IR phosphorylation was no different to that in the diabetic patients. However, insulin-stimulated IRS phosphorylation, insulin-stimulated Akt phosphorylation and insulin-stimulated GSK-3 phosphorylation were reduced in the diabetic patients compared with the normal controls by 24, 43 and 25%, respectively(p<0.05). Conclusion: In Korean type 2 diabetic patients, the insulin resistance may be due to the impairment of the upstream insulin signal molecular network. Further studies will focus on determining whether these signaling defects are the cause of the development of insulin resistance, or secondary to the altered metabolic state, associated with type 2 diabetes mellitus (J Kor Soc Endocrinol 17:685∼697, 2002).

외안각 절제술과 경결막 절개에 의한 안와 접근법

정종철,김성범,서지훈,송민석,전창훈,최세훈,김현민 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.4

Various surgical approaches to the orbits have been used. But the transconjunctival approach, also called the inferior fornix incision, provides adequate access to the orbital floor with esthetic results. Additionally, transconjunctival approach with lateral canthotomy allows more wide surgical field and good access to the orbital area. Generally, preseptal approach is more used rather than postseptal approach in the transconjunctival incision for the prevention of fat herniation during surgery. There are some complications in transconjunctival approaches but these complications can be prevented with careful surgical techniques. We report clinical results in 12 patients who were treated by preseptal transconjunctival incision with lateral canthotomoy for the treatment of orbital trauma. We were satisfied with this technique for reduction and reconstruction of the orbital trauma and we could also observe relatively good esthetic results.

아버지의 양육참여와 유아의 역활수용능력 및 친사회적 행동과의 관계

정현희,최경순 동의대학교 생활과학연구소 1997 生活科學論集 Vol.1 No.-

The purpose of this study was to answer the following questions. (1) Is there any relationship between father's child-rearing behaviors and children's role-taking, between father's child-rearing behaviors and children's prosocial behavior? (2) Is there any relationship between children's role-taking and children's prosocial behavior? (3) Is there any differences in father's child-rearing behaviors, children's role-taking and children's prosocial behavior in relation to children's sex, SES, and mother's job? (4) What are the most important predictor variables which influence on children's role-taking? (5) What are the most important predictor variables which influence on children's prosocial behavior? The subjects of this study were 72boys and 70 girls attending the nursery schools and their mothers in Busan. For the measurement of father's child-rearing behaviors, Choi's(1991) questionnare were used and for children's role-taking children were interviewed with the Flavell's apple-dog stories children's prosocial behavior was measured by amount of candies to share with classmates. The results were as follows : (1) There were significant correlation between father's child-rearing behaviors and children's role-taking, between father's child-rearing behaviors and children's prosocial behavior. (2) Children's role-taking was positively related to children's prosocial behavior. (3) There were significant difference in father's child-rearing behaviors, children's role-taking and children's prosocial behavior according to children's sex, SES, and mother's job. (4) Father's child-rearing behaviors and SES were the most important predictor variables influencing on children's role-taking. (5) Children' s sex, children's role-taking and father's child-rearing behaviors were the most important predictor variables influencing on children's prosocial behavior. The findings stated above seemed father's child-rearing behaviors and children's role-taking, influenced on children's prosocial behavior.

안면비대칭자의 하악골 악교정수술 후 정면 연조직 변화 예측을 위한 연구

황현식,Lee, Jessica J.,황정현,최학희,임회정 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.4

본 연구는 안면비대칭 환자의 악교정수술 시 경조직 이동에 따른 연조직 변화를 정면에서 평가함으로써 정면 얼굴 이미지 시뮬레이션 프로그램 개발에 도움이 되고자 시행되었다. 안면비대칭이 동반된 하악골 수술 예정 환자 45명을 대상으로 정모 두부방사선규격사진과 얼굴사진(photo)을 술전 및 술후에 각각 같은 각도로 촬영한 후, 술전 및 술후의 방사선사진을 이용하여 경조직 계측점의 변화를, 얼굴사진을 이용하여 연조직 계측점의 변화를 수평 및 수직으로 구분하여 각각 측정한 후 경조직 변화에 따른 연조직 변화를 비교 분석하였다. 연조직 변화와 경조직 변화의 상관성을 살펴본 결과 수평 방향, 수직 방향 모두에서 전반적으로 낮은 상관성을 보였으며, 1:1 mean ratio 산출을 위하여 서로 상관성이 가장 높은 경조직 계측점을 연조직 계측점별로 선택한 결과 직하방에 있는 경조직보다는 다소 멀리 떨어져 있는 경조직 계측점이 선택되는 경우가 많이 나타났다. 경조직 변화를 이용하여 연조직 변화를 예측할 수 있는 회귀방정식을 연조직 계측점별로 산출한 결과 연조직 수평변화 예측에 경조직 수직변화도 사용되고 연조직 수직변화 예측에 경조직 수평변화도 사용되었으며, 수평과 수직변화 모두에서 가장 설명력이 높은 방정식은 연조직 menton에서 나타났다. 이상의 연구결과 하부 경조직 계측점과 상부 연조직 계측점의 비율을 이용하는 1:1 mean ratio방법은 불가능한 것으로 나타난 반면 회귀분석을 이용한 연조직 변화 예측은 임상에 도움이 될 수 있는 것으로 나타나 정면 이미지의 경우 컴퓨터를 이용한 시뮬레이션 프로그램이 반드시 필요함을 시사하였다. Objective: To aid the development of a frontal image simulating program, we evaluated the soft tissue frontal changes in relationship to movement of hard tissue with orthognathic surgery of facial asymmetry patients. Methods: Preoperative and postoperative frontal cephalograms and frontal view photographs of 45 mandibular surgery patients with facial asymmetry were obtained in a standardized manner. Vertical and horizontal changes of hard tissue and soft tissue were measured from cephalograms and photographs, respectively. Soft tissue change in result to hard tissue change was then analyzed. Results: Both vertical and horizontal correlation analysis showed a weak relationship between the changes. Hard tissue points that were picked for 1:1 mean ratio with soft tissue points did not show any significant relevance. For each soft tissue change, regressive equation was formulated by stepwise multiple regression analysis, and the equation for soft tissue Menton was most reliable in predicting changes. Both vertical and horizontal hard tissue changes were used together in prediction of vertical or horizontal soft tissue change. Conclusions: The results suggest that computerized image simulation using regression analysis may be of help for prediction of soft tissue change, while 1:1 mean ratio method is not useful.

치커리와 쑥갓의 무균자엽절편체 생산 효율 증진

황현정,전혜정,최근원,김영채 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

치커리와 쑥갓에 있어 식물조직배양이나 형질전환 과정에 필요한 무균 절편체를 얻기 위한 적절한 방법을 제안하기 위해 기내 파종을 위한 소독조건과 배지조성 및 종류에 관한 실험들이 실시되었다. 치커리 'Precole'은 10% NaOCl, 15분간, 'Chiavari'는 5% NaOCl, 15분간이 기내파종 시 발아 및 자엽전개를 촉진시키고 오염발생을 억제하는 조건으로 선발되었다. 쑥갓에서는 발아 및 자엽전개율에 있어 NaOCl 처리 간에 차이는 없었지만 10% NaOCl, 30분간 처리에서 가장 낮은 오염율이 관측되었다. 치커리와 쑥갓의 발아와 자엽전개율 모두 인조토양배지의 사용으로 MS 배지에 비해 현저히 향상 되었다. 비록 육안으로는 오염여부를 관측할 수는 없었지만 이들 인조토양배지에서 자란 쑥갓 묘의 자엽을 MS배지에 치상하여 배양 시에는 오염증상이 나타났으며 이들을 NaOCl 1%로 15분간 소독 후 치상한 결과 오염율을 현저히 낮출 수 있었다. Experiments on the sterilization condition and medium composition and type for in vitro sowing were conducted to suggest a proper way to produce enough aseptic explants for tissue culture or transformation study in chicory and garland chrysanthemum. Fifteen minutes soaking treatment with 10% NaOCl for chicory 'Precole' and 15 minutes with 5% NaOCl for chicory 'Chiavari' were selected as proper sterilization conditions to promote in vitro germination and cotyledon expansion and inhibit the seedling contamination from seed itself. In garland chrysanthemum, there was no significant difference in germination and cotyledon expansion ratio among NaOCl treatments, but the lowest seedling contamination ratio was observed by 30 minutes soaking with 10% NaOCl. Both germination and cotyledon expansion ratio in chicory and garland chrysanthemum were significantly increased by using the artificial soil media in contrast to MS media. Although seedling contamination with the artificial soil media could not be observed with bare eyes, cotyledon explants harvested from the plants grown on the media showed some fungal growth when they were placed onto MS media for further culture. However the percentage of the contamination from these explants was dramatically decreased by 15 minutes sterilization with 1% NaOCl.