연 착화제 효능에 관한 동물실험적 연구

김정만,김병권,김성환,서병성,김인식,김용규,정갑열,김준연 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.1

한국인 갑상선 수질암 환자에서 RET 원종양유전자 점돌연변이 양상

김형훈,김현진,정윤재,민용기,이명식,이문규,김광원,기창석,김종원,정재훈 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.4

연구배경: 갑상선 수질암의 25∼30%는 유전성으로 발현되는데, MEN 2A, MEN 2B 또는 가족성 수질암의 형태로 나타난다. RET 원종양유전자의 점돌연변이가 유전성 갑상선 수질암의 발생에 중요한 역할을 하므로, 진단 당시의 연령이나 가족력 유무에 관계없이 모든 갑상선 수질암 환자나 또는 RET 변이가 발견된 수질암 환자의 가족 구성원들에서 RET 변이 검색을 하여야 한다. 또한 일부 문헌에서 RET 변이의 양상에따라 임상상이 다르게 표현됨이 보고된 바 있다. 이에 저자들은 한국인 갑상선 수질암 환자에서 RET 원종양유전자의 점돌연변이의 양성률을 알아보고, 변이 양상에 따른 임상상의 차이가 있는지를 알아보고자 본 연구를 시행하였다. 방법: 치근 7년간 본원에서 갑상선절제술을 통해 갑상선 수질암으로 진단받은 29예에서 RET 원종양유전자의 점돌연변이 검사를 시행하였다. 29예의 평균연령은 39세(20∼60세)이었고, 남자 7예, 여자 22예 이었다. 이들의 말초혈액에서 genomic DNA를 분리하고, 특이 시발차를 이용하여 RET 원종양유전자의 exon10, 11, 13, 14, 16부위를 증폭하였다. 증폭된 부위를 자동염기서열분석기를 이용하여 직접 분석하였다. 양성으로 나온 경우는 모든 가족 구성원을 대상으로 RET 변이 유무를 검색하였다. 결과: 대상 환자 29예 중 9예 (31%)에서 RET 원종양유전자 점돌연변이가 발견되었다 RET 변이가 발견된 9예 (남자 3예, 여자 6예)의 평균 연령은 33세 (20∼51세)로 RET 변이가 발견되지 않은 20예의 평균연령 42세(24∼60세)보다 의미 있게 적었다. RET 변이가 발견된 9예 중 MEN 2A가 5예, 가족성 수질암이 1예, 그리고 산발성 수질암이 3예이었고, MEN 2B는단 1예도 진단되지 않았다. MEN 2A 5예 중 4예는 exon 11의 codon 634번(C634R 2예, C634Y 2예)에서, 그리고 나머지 1예는exon 10의 codon 618번 (C618R)에서 변이가 각각 발견되었다. 가족성 수질암 1아는 codon 634번(C634W)에서, 산발성 수질암 3예도 모두 codon 634번 (C634y 2예, C634s 1예)에서 각각 변이가 발견되었다. RET변이 양상 또는 위치에 따른 임상상의 차이는 발견할 수 없었다. 결론: 갑상선 수질암 환자 31%에서 RET 원종양유전자의 점돌연변이를 발견할 수 있었다. 유전성 수질암 중 가족성 수질암 1예를 제외하고 나머지 5예는MEN 2A이었다. 저자들의 5예와 지금까지 국내에서 보고 된 7예를 합친 국내 MEN 2A 12예 중 75% (9/12)는 exon 11의codon 634번(C634R 4예, C634y 4예, C634w 1예)에서, 그리고 나머지 25% (3/12)는 exon 10의 codon618번(C618R 2예, C618s 1예)에서 변이가 발견되었다. 국내에서는 codon 634과 codon 618 두 곳에만 국한된 양상이었고, codon 634에서의 C634R 변이는 1/3에서만 나타났다. 비록 본 연구에서는 제한된 환자 수 때문에 변이 양상과 임상상의 관계를 규명할 수 없었지만, 향후 많은 수의 환자를 대상으로 전향적인 연구를 시행하여 genotype-phenotype 관계 규명을 하는 것이 필요하다. Background: Medullary thyroid carcinomas (MTC) have been reported as hereditary in about 25 -30% of cases. The identification of germline mutation in RET proto-oncogene is important in the diagnosis of hereditary MTC, and occurs in three forms: MEN 2A, MEN 2B and familial MTC (FMTC). To evaluate the prevalence of the relationship of RET proto-oncogene mutation and genotype-phenotype was studied in Korean patients with MTC. Methods: Genomic DNA was obtained from 29 patients, with MTC, who underwent a total thyroidectomy, between 1997 and 2003, at the Samsung Medical Center. There were 7 male and 22 female patients, with an average age of 39, ranging from 20 to 60 years. Exon 10, 11, 13, 14 and 16 of the RET proto-oncogene were amplified, with specific primers, using PCR. A sequencing analysis was performed on the PCR product using an automatic sequencing analyzer. Results: Nine of the 29 patients (31%) were identified as having RET mutations. The average age of these 9 patients was 33 years, ranging from 20 to 51, with a female to male ratio of 2. Five patients had MEN 2A and one had FMTC, with the other 3 thought to have non-hereditary (sporadic) MTC. The 4 patients with MEN 2A had RET mutations on codon 634 of exon 11 (2 patients, C634R; 2 patients, C634Y) and the other patient on codon 618 of exon 10 (C618R). One patient with FMTC had a mutation on codon 634 (C634W). Three patients with sporadic MTC had RET mutations on codon 634 (2 patients, C634Y; 1 patient, C634S). However, no genotype- phenotype relationship could be found, due to the limited number of patients. Conclusion: Thirty-one percent (9/29) of the patients with MTC had RET proto-oncogene mutations. Three-quarters (9/12) of the Korean patients with MEN 2A, including another 7 patients reported in 3 papers in Korea, had RET mutations on codon 634 of exon 11 (4 patients, C634R; 4 patients, C634Y; 1 patient, C634W), but a quarter (3/12) had mutations on codon 618 of exon 10 (2 patients, C618R; 1 patient, C618S). Although no relations could be found between the genotypes and phenotypes, extensive prospective studies will be required to verify this (J Kor SOC Endocrinol 18:360-370, 2003).

연 착화제 효능에 관한 동물실험적 연구

서병성,김준연,김정만,김용규,정갑열,김성환,김인식,김병권 동아대학교 부설 산업의학연구소 1997 산업의학연구소 논총 Vol.- No.3

This experiment was carried out to compare the efficacy of 2,3-dimercaptosuccinic acid(DMSA) and calcium ethylenediamine tetraacetate(CaEDTA) as chelating agents for lead poisoning. The rats had been fed water containing 0.5% lead acetate for 3 months and randomly divided into three groups. We administered DMSA only for group I(n=5), CaEDTA only for group II(n=5), and both DMSA and CaEDTA for group III(n=10), for 4 days. We evaluated urinary lead(PbU) level, blood lead(PbB) level and blood zinc protoporphyrin(ZPP) level before and after administration of the chelating agents. The results obtained were as follows; 1. After administration of chelating agents, the mean PbU levels, which were 248.2㎍/dl, 252.6㎍/dl and 270.0㎍/dl in group I, II and III before administration, were increased to 484.6㎍/dl, 479.9㎍/dl and 1,792.1㎍/dl in group I, II and III, respectively(p<0.05). 2. After administration of chelating agents, the mean PbB levels, which were 67.4㎍/dl, 59.9㎍/dl and 62.5㎍/dl in group I, II and III before administration, were decreased to 38.1㎍/dl, 36.3㎍/dl and 31.3㎍/dl in group I, II and III, respectively(p<0.05), but there were no different changes of ZPP level among groups. 3. The blood lead clearances, which were calculated by PbB level before and after administration of chelating agents, were 49.8%, 43.5% and 39.5% in group III, I and II, respectively.

분만 전·후 사료급여 형태가 젖소의 생산성에 미치는 영향

기광석,김현섭,이왕식,이현준,김상범,정하연,은정식,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This experiment was carried out to protect drops of feed intake should be plural prepartum and postpartum to reduce metabolic diseases after calving to know how feeding systems, which is divided as a low quility, a high quility and total mixed rations(TMR), affects on postpartum productivity of Holistein cows. Three diets (low or high quality forage separately fed with concentrate and TMR containing high quality roughage) were fed to 21 cows from 3 weeks prepartum to 8 weeks postpartum to examine their effects on the productivity of cows. DM intakes was noticed significantly higher with TMR (17.11kg/day) than low- quality (13.48 kg/day) and high-quality forage (13.10kg/day). TDN and CP intakes were also higher with TMR compared to other experimental diets. Mean daily milk yield was non-significant among the cows fed different diets. Blood non-esterified fatty acids(NEFA) content was higher in cows fed low-quality or high-quality forage separately with concentrate compared with those fed TMR. The results concluded that TMR feeding to transitional cows is better than feeding the low or high quality forage separately for their health and productivity. 본 시험은 분만 전 3주부터 분만 후 3주까지 전환기라고 지칭되는 시기의 젖소들에 대한 대사적 변화를 최소화하기 위한 방법으로 사료 섭취량 저하를 방지하고 분만 후 대사성 질병의 발생을 줄이기 위한 목적으로 수행되어졌다. 대사성 질병에 관여하는 주요 요인으로는 건물섭취량, 혈중 glucose 함량, NEFA 함량, 반추위 환경, 면역 등인데, 분만 전·후 Holstein 젖소에 사료급여 형태를 조·농분리(저질 조사료와 양질 조사료 급여구) 및 TMR로 다르게 하였을 때 사료섭취량 변화와 혈중 NEFA 및 glucose 함량 변화를 측정하고 분만 후 젖소의 생산성에 미치는 영향을 구명하기 위하여 수행하였다. 처리별 사료건물 섭취량은 저질 조사료 조·농 분리, 양질조사료 조·농 분리, TMR 급여구에서 각각 13.48, 13.10, 17.11kg으로 TMR 급여구에서 유의성 있게 높았으며(P〈0.05), TDN 섭취량은 처리구별 각각 7.35, 7.19, 5.66kg으로 처리간에 차이가 없었다(P〉0.05). 신체충실지수(BCS)는 저질조사료구에서는 3.03이였으며, 양질 조사료구와 TMR 급여구에서는 각각 3.19, 3.16이었다. 분만후 1주부터 5주까지 측정한 평균 산유량은 저질조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 34.1, 35.1, 35.6kg 였으며, 분만 후 4∼5주에 최고 비유기에 달했다. 처리별 평균 유지율은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 3.74, 3.68, 3.95%였다. 혈청내 NEFA 함량은 저질 조사료 조·농 분리, 양질 조사료 조·농 분리, TMR 급여구에서 각각 744.4, 381.8,266.7μEq/L로 처리간에 큰 차이를 보여 양질 조사료 급여와 TMR 급여시 혈중 NEFA 함량이 저질 조사료 급여에 이해 유의성 있게 낮았다(P〈0.05). 혈청내 glucose함량은 저질조사료 및 양질 조사료 조·농 분리, TMR 급여구에서 각각 39.3, 56.3, 62.4mg/dl로 양질 조사료 급여와 TMR 급여시 혈중 glucose 함량이 저질 조사료급여에 비해 유의성 있게 높았다(P〈0.05). 이상의 결과들을 종합해 볼 때, 분만 전 3주부터 분만 후 3주까지의 전환기 동안 사료섭취량 증가와 대사성 질병의 지표로서 혈중 NEFA 및glucose 함량 변화를 살펴 본 결과 분만 전 3주부터는 양질의 조사료나 TMR 형태로 급여하는 것이 사료섭취량 증가와 대사성 질병의 발생을 감소시킬 수 있을 것으로 사료되었다.

체질분류에 기초한 감성공학

김원식,이용호,박찬복 한국정신과학학회 1999 韓國精神科學學會誌 Vol.3 No.2

지구환경의 영향에 의존하여 특정되어 가는 인간의 기질을 고찰하였고, 이러한 기질의 차이에 따라 동일한 제품 환경에 대하여도 서로 다른 감성을 느끼게 됨을 추론하였으며 체질론에 기초하여 체질에 기질이 가장 잘 반영되어있다고 유추할 수 있어서 각 체질에 적합한 제품·환경 설계를 위하여 과학적인 채질 분류와 모델을 제안하였다. A person’s temper is characterized by the influence of his(her) earth environment- Depending on the various tempers, different human sensibilities can be evoked from the same manufactured goods. Based on the hypothesis of habitude in which the temper is related intimately, we suggested a model of classifying habitudes In order to design a product which can be compatible with the habitude of its user.

중원지역의 설화를 동화화하기 위한 연구 및 개발

김정란,유소영,박혜숙,박용식 건국대학교 중원인문연구소 1999 중원인문논총 Vol.19 No.-

This study describes the research and development of fairy tales from the folk tales in Joung-Won area. The need for this problem was assessed through the special training course of fairy tale writing sponsored by Cho-Bang. The first step was the study of the suitable definition and types of fairy tales. Assuming that the problem in defining the field of folk tales is the lack of common agreement in the labels, definitions and standard classification models of folk tales, literature related to these areas were reviewed. The application of this research and development will be the fairy tale writing. The next step was therefore to select the folk tales suitables to the fairy tale writing and to explore the Joong-Won area related to these stories. By this study we found that Joon-Won area's folk tales have few supernatural, fantastic elements which are the common characteristics of fairy tales. Considering the lack of these characteristics, we selected and wrote the story of "General Shin Lip", "the funny stories", "the story of the Castle Mago"(from the Nam mountain folk tales), "princess Ari and prince Koko"(from the folk tales of Kye-Myung mountain), etc... We found the development of the fairy tales a meaningful and rewarding work and experience. Our purpose to gain the basic knowledge in the development of this field has been met. But these fairy tales need to be visualized to be published. We should prepare for the implementation of practical application of this study and development: contact with a higher administrator in Joong Won area, discussion on the support possibility, visual production, addition of necessary materials, etc... Only the surface has been touched in this study. But the authors hope that in the near future, we will be able to create the new cultural work needed in the Joon-Won area.

컴퓨터 自動化 生産시스템 導入 戰略에 관한 硏究

金容式 圓光大學校大學院 1990 論文集 Vol.5 No.-

Modern enterprises are understood as organisms which survive in the dynamic aociety. This means that they cannot live or evolve if they fail to adapt effectively to the organizational environment. Managers are interested in the automation of their systems as one of major business strategies to cope with consummer's needs which are getting various and the market share which is undergoing obviously under uncertainty. Factory Automation(FA) which was started with Numerical Control machines is expected to realize the peopleless factory in the near future as micro computers, industrial robots and Flexible Manufacturing Systems(FMS) with Cmputer-Aided Design(CAD), Computer-Aided Manuf-acturing(CAM) and Computer-Aided Engineering(CAE) have been introducing into the organizations. The main objectives of this study are to introduce the basic theory on Manufacturing Automation and the models to evaluate the projects for it and to present basic strategic guidelines to help the managers who are considering with inplementing Computer-Automated Manufacturing Systems. Major findings through this study are as follows; 1. Computer-Sutomated Manufacturing Systems should be designed in the point of view that the managerial departments as well as manufacturing departments are included. Thus Top-down approach must be performed. 2. The evaluation simply by cost-benifit analysis is not sufficient to accept the project because the great amount of cost and the long period of return on investment is necessary, and the effect is difficult to be measured as the system affects the whole organization. Therefor new models which are considered as a long-run and strategic point have been recommended. Futher study is necessary to prove that the findings are acceptable to Korean firms, on which the empirical or case study should be included. The production systems for the manufacturing industry in Korea are far behind those in advanced countries, such as The United States of America and Japan, on the aspect of system technology. Nowadays, Korean firms are being suffered from the impression for high wage and the rapid appreciation of WON(Korean monetary unit) against US dollars( $ ). These enable the managers in our counrtry to take account of Factory Automation with high flexibility to market's environment. Acceptable theories and guidelines are necessary to help them to introduce the new system into their firms.

삼첨판 기계식 인공판막 대치술을 포함한 세 차례 개심술을 시행한 환자에서 대심정맥을 통한 영구 박동조율기 이식 1예 보고

김재욱,이진호,최주원,김우식,김용인 인제대학교 백병원 2003 仁濟醫學 Vol.24 No.2

A 26-year-old man. who had undergone three times open heart surgeries including tricuspid mechanical valve replacement 4 months before, with general weakness and mild dyspnea was admitted. He presented a third degree atrioventricular block on the electrocardiogram and required permanent pacemaker implant. We successfully implanted a permanent pacemaker transvenously with a ventricular lead introduced into the great cardiac vein via coronary sinus to stimulate the left ventricle, an atrial lead in the right atrium, and his symptoms improved.

情報시스템要員 및 END-USER의 敎育訓練需要에 관한 硏究 : 敎育/訓練의 有用性/熟達程度/不足程度 分析

金容式 順天靑巖大學 1994 論文集-順天靑巖大學 Vol.18 No.-

Education and training in information technology may be a critical factor for most business organizations in the era that national boundaries does not exist in worldwide market. The quality and efficiency of information systems including applications is very important for organizations to achieve their own objectives. Information system staff and End-Users should cooperate each other to produce better applications. But, both group alike may suffer from a gap of a different kind: a lack of understanding of the organization and its environment. The best way to solve the problem is to provide those with a sophisticate education/training system. The main objective of this paper is to review preperformed studies and practical results of empirical investigations which have mostly been conducted in advanced countries. This kind of works could help the practitioners to evaluate the needs of education/training and to develop their human resources effectively. Several suggestions are indicated in this paper and are summarized as follows; 1. Improve information system literature of all employees. 2. Provide IS staff with more management-related education. 3. Provide End-Users with more skills of Information technology. 4. Educate both group to cooperate more closely. 5. Evaluate the needs of education/training in the view of deficiency. The empirical study should be conducted on the base of the conclusions. For this, the instruments verified with reliability test must be developed by referring to Nelson's questionnaire and modifying it according to the present evolutional stage of End-User computing in Korea.

건물의 가압방연시스템 설계를 위한 유동해석에 관한 연구

김명배,한용식 社團法人 韓國火災 ·消防學會 2000 한국화재소방학회논문지 Vol.14 No.2