Panipenem을 포함한 Carbapenem 계열 항생제의 몇 가지 임상균주에 대한 시험관내 항균력

이동건,김명신,강진한,전혜선,최수미,위성헌,김상일,최정현,유진홍,신완식,강문원 대한감염학회 2003 감염과 화학요법 Vol.35 No.2

목적 : Panipenem은 일본에서 개발된 카바페넴계 항생제로 그람양성균, 그람음성균, 혐기균에 이르기까지 광범위한 항균력을 나타낸다고 보고되고 있지만 국내에서 분리되는 균주에 대한 감수성 검사결과는 아직 없다. 본 연구에서는 임상에서 분리된 E. coli, K. pneumoniae, P. aeruginosa, S. pneumoniae 균주를 대상으로 carbapenem 계열 및 몇 가지 항생제와 감수성을 비교하고, 특히 P. aeruginosa의 경우 carbapenem의 효과를 Muller-Hinton agar (MHA)가 반감시킬 수 있는 특성을 고려하여 감수성에 변화가 있는지 확인하고자 하였다. 방법 : 2002년 3월부터 6월까지 가톨릭대학교 성모병원, 강남성모병원, 성가병원, 성모자애병원, 성빈센트병원에서 분리된 임상균주 E. coli, K. pneumoniae, P. aeruginosa, S. pneumoniae를 대상으로 하였다. 항생제 감수성 검사는 NCCLS 기준에 따랐고 P. aeruginosa의 경우 MHA 이외에 minimal agar Davis (MAD)를 추가로 사용하였다. 감수성 결과 해석시 panipenem은 imipenem과 같은 기준을 적용하였다. 결과 : E. coli, K. pneumoniae, P. aeruginosa는 각각 100균주였고, S. penumoniae는 134균주였다(PSSP 41, PNSP 93균주). E. coli, K. pneumoniae는 모든 균주가 imipenem, meropenem, panipenem에 대해 감수성을 보였다. P. aeruginosa의 경우 imipenem, meropenem, panipenem, ceftazidime의 MIC_(90)은 각각 16, 8, 32, 64㎍/mL이었다. MHA와 MAD 배지를 이용하여 P. aeruginosa에 대한 항생제 감수성을 비교해 본 결과 imipenem, panipenem의 MIC_(90)가 각각 16, 32㎍/mL에서 8㎍/mL로 감소하였고, imipenem에 비해 panipenem이 더 많이 감소하였다. S. pneumoniae의 경우 PSSP는 imipenem, meropenem, panipenem에 대해 모두 내성이 없었고 PNSP는 imipenem, meropenem, panipenem에 대한 MIC_(90)가 각각 1, 2, 0.25㎍/mL이었다. 결론 : 결론적으로 panipenem은 E. coli, K. pneumoniae에 대해 기존의 카바페넴계 항생제와 유사한 우수한 항균력을 보였다. P. aeruginosa의 경우 ceftazidime, imipenem과 비슷한 항균력을 보였고 아미노산 함량이 적은 배지를 사용함에 따라 MIC가 낮아졌으며 imipenem보다 더 많은 영향을 받았다. S. pneumoniae의 경우 특히 PNSP에서 기존의 카바페넴계 항생제보다 우수한 항균력을 보였다. Panipenem은 주요 그람음성균 및 폐렴연쇄구균 감염에 유용할 것으로 기대된다. Background : Panipenern (PAPM) is a new carbapenern which has an enhanced broad spectrum against both gram-positive and negative organisms. The aim of study was to compare the activities of PAPM with those of imipenern (IMPM), meropenern (MRPM) against several clinical isolates in Korea. Methods : We tested the in vitro antimicrobial activities of PAPM, IMPM, and MRPM against total 300 clinical isolates of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa and 134 Streptococcus pneumoniae collected from 5 different university hospitals. Using NCCLS guidelines, MICs of PAPM, IMPM, MRPM, and/or ceftazidime were determined. Results : All isolates of E. coli and K. pneumoniae were susceptible to PAPM, IMPM and MRPM. MIG_(90) of PAPM, IMPM, and MRPM against P. aeruginosa were 32, 16, and 8 ㎍/mL, respectively. Comparing Mueller-Hinton agar (MHA) and minimal agar Davis (MAD), the MIG_(go) of PAPM and IMPM were reduced from 16 and 32 ㎍/mL to 8 and 8 ㎍/mL, respectively. PAPM was more influenced by MAD than IMPM. All isolates of penicillin susceptible S. pneumoniae showed 0% of resistance to the carbapenems tested. MIC_(90) of PAPM, IMPM and PRPM against penicillin non-susceptible S. pneumoniae were 0.25, 1 and 2 ㎍/mL, respectively. Conclusion : Panipenern could be one of the potentially useful drugs for the treatment of infections caused by E. coli, K. pneumoniae, and S. pneumoniae. We also showed that PAPM had good anti-pseudomonal activity when examined in MAD, the amino acid-limited media. Therefore, it could be useful for the treatment of infections caused by P. aeruginosa.

원저 : 재치료 폐결핵환자의 임상적 세균학적 특성

오승준 ( Seoung Joon Oh ),윤기헌 ( Ki Heon Yoon ),유지홍 ( Jee Hong Yoo ),강홍모 ( Hong Mo Kang ) 대한결핵 및 호흡기학회 1995 Tuberculosis and Respiratory Diseases Vol.42 No.1

Prepenem과 Carbapenem 항생제의 몇가지 임상균주에 대한 시험관내 항균력 비교

유진홍,이동건,김명신,강진한,전혜선,송민진,최수미,위성헌,김상일,최정현,신완식,강문원 대한감염학회 2003 감염과 화학요법 Vol.35 No.6

흡연력과 폐기능에 대한 혈중 호중구 , 호산구 및 호산구성 양이온 단백의 관계에 관한 연구

박병규(Byung Kyu Park),김준홍(Jun Hong Kim),고원욱(Won Ook Ko),이준상(Jun Sang Lee),성낙현(Nak Heon Seoung),박승근(Seung Keun Park),박순규(Soon Kew Park),신영기(Young Kee Shin),이인태(In Tae Lee),박정현(Jung Hyun Park),김치학(Chi Hak 대한내과학회 1997 대한내과학회지 Vol.52 No.2

N/A Objectives: A prospective study was performed to find out the correlation between neutrophil, eosinophil and eosinophilic cationic protein (ECP) the granular protein in eosinophil - and smoking history and pulmonary function change, Methods: In this study there were seventy smokers and seventy lifetime nonsmokers. None of them had the history of atopy, or the evidence of parasitic infestation. Smoking history, spirometric pulmonary function test and blood samples for CBC, serum ECP were analyzed. Results: 1) Eosinophil and neutophil count was elevated in the smokers compared with the nonsmokers. 2) Serum ECP was elevated in smokers and the magnitude of this increase was greater than that of eosinophil count. 3) In the smokers, there was a direct proportional correlation between serum ECP and FEV1, but inverse proportional correlation between neutophil count and FEV. 4) In the smokers, neutrophil count was elevated regardless of FEV value. 5) The level of serum ECP well correlated with the daily tobacco consumption but not with the duration of smoking or pack-year consumption. Conclusion: Incresed neutrophil count, decreased eosinophil count and decreased serum ECP level could be useful in the understanding of pathophysiology of lung parenchymal destruction in smokers.

Tocotrienol regulates osteoclastogenesis in rheumatoid arthritis

( Kyoung-woon Kim ),( Bo-mi Kim ),( Ji-yeon Won ),( Hong Ki Min ),( Seoung Joon Lee ),( Sang-heon Lee ),( Hae-rim Kim ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.0

Background/Aims: The present study aimed to investigate whether tocotrienol regulates interleukin 17 (IL-17)-induced osteoclastogenesis in rheumatoid arthritis (RA). Methods: We evaluated the effect of tocotrienol on IL-17-induced receptor activator of nuclear factor kappa B ligand (RANKL) production using RA fibroblast-like synoviocyte (FLS), together with real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Osteoclast differentiation was confirmed after culturing IL-17-treated RA FLS and Th17 cells with tocotrienol and monocytes. We analyzed the suppressive effect of tocotrienol on Th17 cells percentage or Th17-cytokine levels among peripheral blood mononuclear cells using flow cytometry. Results: We found that IL-17 stimulated FLS to produce RANKL and tocotrienol decreased this IL-17-induced RANKL production. Tocotrienol decreased the IL-17-induced activation of mammalian target of rapamycin, extracellular signal-regulated kinase, and inhibitor of kappa B-alpha. When monocytes were incubated with IL-17, RANKL, IL-17-treated FLS or Th17 cells, osteoclasts were differentiated and tocotrienol decreased this osteoclast differentiation. Tocotrienol reduced Th17 cell differentiation and the production of IL-17 and sRANKL; however, tocotrienol did not affect Treg cell differentiation. Conclusions: Tocotrienol inhibited IL-17- activated RANKL production in RA FLS and IL-17-activated osteoclast formation. In addition, tocotrienol reduced Th17 differentiation. Therefore, tocotrienol could be a new therapeutic choice to treat bone destructive processes in RA.

Biological Activity of Bamboo Salt

Shin, Hye-Young,Na, Ho-Jeong,Moon, Phil-Dong,Seo, Sang-Wan,Shin, Tae-Yong,Hong, Seoung-Heon,Lee, Ki-Nam,Park, Rae-Kil,Kim, Hyung-Min The Korean Society of Food Science and Nutrition 2004 식품산업과 영양 Vol.9 No.1

Bamboo salt has been used for the purpose of prevention and treatment of various diseases in Korea. Present study was carried out to ascertain the effects of purple bamboo salt upon anti-allergic effect, anti-inflammatory activity and immune-enhance effect as well. Purple bamboo salt significantly inhibited the ear swelling response and histamine release induced by compound 48/80 in mice and rat peritoneal mast cells. Purple bamboo salt (0.01 ∼ lg/kg) also dose-dependently inhibited the passive cutaneous anaphylaxis by oral administration. Purple bamboo salt (1 mg/mL) in hibited phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-1${\beta}$ and IL-6 secretion, by 67.04${\pm}$0.08%, 68.01${\pm}$1.85%, 69.48${\pm}$0.54%, respectively. In addition, purple bamboo salt inhibited the expression of TNF-${\alpha}$ mRNA in HMC-1 cells. Finally, we investigated the effect of purple bamboo salt in the forced swimming test (FST) and the change of purple bamboo salt-mediated cytokine production from MOLT-4 cells. At the 7th, immobility time was significantly decreased in the purple bamboo salt-administration group (35.4 ${\pm}$5.9 s for 1 g/kg) in comparison with the control group (93.2 ${\pm}$ 15.45). After FST, the content of glucose in the blood serum was increased and the levels of blood urea nitrogen, lactic dehydrogenase was decreased in purple bamboo salt-administration group. However, it had no effect on the elevation of CK and TP level. Purple bamboo salt (1 mg/mL) significantly increased the interferon (IFN)-${\gamma}$ and IL-2 level compared with media control (about 3.7-fold for IFN-${\gamma}$, about 3.5-fold for IL-2, p〈0.05) but did not affect the IL-4.

[특집 : 질병예방과 영양ㆍ의약식품] Biological Activity of Bamboo Salt

Hye-Young Shin,Ho-Jeong Na,Phil-Dong Moon,Sang-Wan Seo,Tae-Yong Shin,Seoung-Heon Hong,Ki-Nam Lee,Rae-Kil Park,Hyung-Min Kim 한국식품영양과학회 2004 식품산업과 영양 Vol.9 No.1

Bamboo salt has been used for the purpose of prevention and treatment of various diseases in Korea. Present study was carried out to ascertain the effects of purple bamboo salt upon anti-allergic effect, anti-inflammatory activity and immune-enhance effect as well. Purple bamboo salt significantly inhibited the ear swelling response and histamine release induced by compound 48/80 in mice and rat peritoneal mast cells. Purple bamboo salt (0,01~1 g/㎏) also dose-dependently inhibited the passive cutaneous anaphylaxis by oral administration. Purple bamboo salt (1 ㎎/mL) inhibited phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated tumor necrosis factor (TNF)- α, interleukin (IL)-1β and IL-6 secretion, by 67.04±0.08%, 68.01±1.85%, 69.48±0.54%, respectively. In addition, purple bamboo salt inhibited the expression of TNF- α mRNA in HMC-1 cells. Finally, we investigated the effect of purple bamboo salt in the forced swimming test (FST) and the change of purple bamboo salt-mediated cytokine production from MOLT-4 cells. At the 7th, immobility time was significantly decreased in the purple bamboo salt-administration group (35.4±5.9 s for 1 g/㎏) in comparison with the control group (93.2±15.4 s). After FST, the content of glucose in the blood serum was increased and the levels of blood urea nitrogen, lactic dehydrogenase was decreased in purple bamboo salt-administration group. However, it had no effect on the elevation of CK and TP level. Purple bamboo salt (1 ㎎/mL significantly increased the interferon IFN)- γ and IL-2 level compared with mediacontrol (about 3.7-fold for IFN- γ, about 3.5-fold for IL-2, p<0.05) but did not affect the IL-4.

췌장의 유두상 낭성종양

박두호(Doo Ho Park),조현미(Hyun Mi Cho),최상욱(Sang wook Choi),서은주(Eun Joo Seo),이성(Seong Lee),홍승덕(Seoung Dug Hong),강동헌(Dong Heon Kang) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.3

Papillary cystic neoplasm of the pancreas is a rare tumor and is mostly found in young women. Grossly, it is usually large and well demarcated and on cross section contains widespread areas of hemorrhage and necrosis. Microscopically, it is composed of papillary and solid pattern. Ultrastructurally, evidence of acinar, ductal, and (sometimes) endocrine cell differentiation has been found. The treatment is surgical and the prognosis is excellent, although a few cases resulting in local recurrence and liver metastasis have been reported. The accumulated experiences indicate that this neoplasm should be regarded as a low-grade, potentially malignant tumor. The authors experienced a case of the papillary cystic neoplasm of the pancreas and so report it with reviews of the references.

목 건강 고위험군의 움직이는 모니터 사용에 따른 두부전방 자세 교정 효과에 관한 연구

최경희(Kyeong-Hee Choi),공용구(Yong-Ku Kong),심현호(Hyun-Ho Shim ),조민욱(Min-Uk Cho),박채원(Chae-Won Park),홍보람(Bo-Ram Hong),김승훈(Seoung-Hoon Kim),정현주(Hyun-Ju Jung),송현아(Hyun-A Song),류지헌(Ji-Heon Ryu),김재경(Jae-Kyeong Ki 대한인간공학회 2019 대한인간공학회 학술대회논문집 Vol.2019 No.10

Objective: The aim of this study was to verify the correction effect of Forward Head Posture (FHP) by using Moving Monitor in a high-risk group of the neck. Background: Office workers who have been used VDTs are subject to high loads on the neck and shoulders during their work due to the high frequency of FHP. Thus, our research team developed a Moving Monitor Arm that continuously induced calibration movements when the users use the monitor. Method: Seven participants who rated as a high-risk group of the neck to study the effect of FHP correction using the Moving Monitor. The fatigue and posture of the neck were quantified using Flexion Relaxation Ratio (FRR), Onset Angle, Offset Angle, Craniovertbral Angle (CVA) and Subjective Discomfort Rating in this study. Results: The effects of task pre/post on the FRR and subjective discomfort, and the effect of monitor type on the Onset/Offset angles was significant in this study, respectively. Conclusion: Based on the results of the experiment, correction effect of Moving Monitor was verified. Application: The results of this study showed that moving monitors can be applied to prevent musculoskeletal disorders associated with VDTs.