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Trichoderma viride에서 분리한 Endoglucanases와 Cellulose의 상호작용에 있어서 Enzyme에 존재하는 Trytophan기의 역할
원성미,장영훈,김동원,정영규,류해일 공주대학교 사범대학 과학교육연구소 1996 과학교육연구 Vol.27 No.1
곰팡이 Trichoderma viride 셀룰라아제로부터 세가지의 endoglucanase (endoglucanase Ⅰ,Ⅱ및Ⅲ)가 다양한 크로마토그래피 과정을 통해 분리되었다. 또한 활성에 미치는 tryptophan기의 역할을 화학적 변형을 통하여 알아보았다. N-bromosuccinimide에 의한 효소의 화학적 변형으로부터, endoglucanase Ⅰ,Ⅱ및Ⅲ의 분자당 지니고 있는 tryptophan의 개수가 각각 6,1,1.5개 임을 알수 있었다. 즉 endoglucanase Ⅰ은 분자당 여섯 개의 tryptophan을 함유하고 있으며, 활성부위에는 한 개가 영향을 미치는 것으로 나타났다. 반면에 endoglucanase Ⅱ및Ⅲ는 분자내에 대략 하나의 tryptophan이 단지 활성부위에서만 작용하는 결과를 보여주었다. 또한 endoglucanase Ⅰ의 경우 활성부위가 아닌 다른 부분에 있는 tryptophan기가 기질의 표면에 흡착하는데 미치는 영향을 알아보았다. Three endoglucanases (endoglucanase Ⅰ,Ⅱ and Ⅲ ) as major cellulase components were isolated from the fungus Trichoderma viride by a of chromatography procedures, and the role of tryptophan residues in the activity towards carboxymethyl cellulose were investigated through kinetic and chemical modification studies. Through the modification of cellulase with N-bromosuccin-imide, it was found that endoglucanase Ⅰ,Ⅱ and Ⅲ contained 6, 1, and 1.5 tryptophan residue(s) per enzyme molecule, respectively. From the result of the kinetic data on the modification, it was estimated that the active site of enzyme includes at least one or two tryptophan residue. These results indicated that, in the case of endoglucanase Ⅰ, five tryptophan residues are exposed to another region of the enzyme except that of active site. In the case of the endoglucanase Ⅱ and Ⅲ, however, tryptophan residue exists almost in the active site. In addition, the relationship between endoglucanase activity towards cellulose and the role of tryptophan residue exposed to the enzyme surface was discussed.
류석용,김원율,김경환,이상래,이경호,김홍용,이승한 대한응급의학회 2000 대한응급의학회지 Vol.11 No.2
Background: The prehospital emergency medical system(EMS) for 119 rescue has progressed considerably, but leaves much to be desired. To improve prehospital EMS, we need to evaluate prehospital patient care, and reeducation. Methods: The records of 1,882 patients, who visited Sanggye Paik Hospital Emergency Department via 119 rescue from April 1 to July 30, 1999, were analyzed according to the quality of patient care as documented by the 119 rescue protocol and database. Patients are grouped into 5 classes based on the quality of the care received. Class 1A received adequate care, class 1B received inadequate care, class 1C did not receive the necessary care, and class 2A received unnecessary care, class 2B did not receive unnecessary care. The results of such analyses are presented to the fireman at monthly meetings, thus reeducating them. Results: The overall results were 1027 cases in Class 1A(55%), 83 in 1B(4%), 149 in 1C(8%), 21 in 2A(1%), and 602 in 2B(32%). Well performed care(1A+2B) occured in 1629 cases(87%) and badly performed care(1B+1C+2A) occured in 253cases(13%). Well perfomed care gradually increased from April to July (April 83%, May 85%, June 87%, and July 89%). Conclusion: Meticulous appraisal of the quality of prehospital patient care and reeducation through the regular meetings of EMS physicians and firemen in each community is needed for developing a model protocol for indirect retrospective medical control of the prehospital EMS.
제7차 교육과정에 따른 국어과 읽기·쓰기 수행평가 연구(Ⅰ)
류성기,곽재용,천경록,이원희 한국초등국어교육학회 2002 한국초등국어교육 Vol.20 No.-
이 연구에서는 제7차 교육과정에 따라 초등학교에서 읽기와 쓰기의 수행평가를 실제적으로 적용하는 방안에 대하여 연구하였다. 제7차 국어과 읽기 교과서에서 '되돌아보기' 학습 면을 둔 것은 전체적으로 수행평가를 현실화하는데 긍정적 기여를 하고 있으나 실제로 분석해보니 단원별로 부족한 부분이 많았다. 따라서 이 연구에서는 되돌아보기 학습면을 보완한 학습지와 성취기준, 평가기준, 평가기록표를 개발하였다. 이 방안을 초등학교 교실에 한 학기 동안 적용하고, 수행평가의 현장 적용 가능성과 교수·학습 개선여부를 살펴보았다. 연구 방법은 개발 연구, 사례연구, 질적 연구, 관찰, 면담, 전문가 협의회 개최등의 방법을 사용하였다. 연구 결과, 교과서 되돌아보기를 보완한 읽기 수행평가 방안은 학생의 읽기 발달에 관한 질적 정보를 수집하는 방법이라는 결론을 얻었다. This paper have developed performance assessment strategies for reading and writing as the 7th Korean National Curriculum, and applied them one elementary school 6th grade classroom during one semester. There is review pages in every reading textbook chapters of 7th korean national curriculum. This contribute to performance assessment. But some of them is not so good. So we developed common tools, achievement standards, assessment standard, report card to compensate review pages, and to apply performance assessments. Method of study is case study, developing study, qualitative study, observation, interview, and panel conference. As a results, this performance assessment strategy could many good developmental informations of students.
韓國産 잎벌亞目(벌目)에 대한 系統分類學的 硏究 Ⅳ. 즐잎벌亞科에 대한 走査電子顯微鏡的 硏究
柳聖萬,崔元煐,李鍾郁 嶺南大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.13 No.-
As a result of this study the Korean Selandriinae consist of 16 species, 7 genera including 4 newly recorded species, Strongylogaster macula, Aneugmenus temporalis, A japonicus and A. coronatus, from Korea.
Emulsion형 化粧品의 Escherichia coli 汚染防止에 대한 防腐制效果
柳美淑,金樟圭,元省皓,金男紀 成均館大學校 科學技術硏究所 1992 論文集 Vol.42 No.2
Most of cosmetics are emulsion products that contain the source of nutrition-vegetable oil, mineral oil and carbohydrate etc. -so there is much possibility of contamination by microbials. The purpose of this study is to get the data necessary not only to prevent dermatopathia occurred by Escherichia coli but to sustain the quality and the wear of cosmetics as the result of finding optimum growth condition and the growth condition of Escherichia coli for enviro-factor, utilizing antiseptic so as to prevent contamination by observing it in emulsion type cosmetic. The results are as follows : 1. The optimum growth condition of Escherichia coli : pH7.0 and 37.5℃ The mixing volume ratio of water / oil was 70/30 in this experiment. 2. Adding antiseptics into emulsion type cosmetic, the number of Escherichia coli was decreased from 10^8(cell/ml) to 8 x 10^3(cell/ml) 3. Antibacterial effect against Escherichia coli was better as follows, p-hydroxy benzoic acid propyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid buthyl ester + acetic acid buffer solution > p-hydroxy benzoic methyl ester + phosphoric acid buffer solution > p-hydroxy benzoic acid methyl ester + p-hydroxy benzoic acid buthyl ester >p-hydroxy
퇴행성 슬관절염 환자 평가 설문도구의 내적 신뢰도, 상관성에 관한 연구
류성룡,안경애,서병관,강중원,이상훈,이재동,최도영,김건식,이두익,이윤호 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-
Objective : The aim of this study is to evaluate internal reliability and correlation among assessment instruments of knee OA. Methods : Data were obtained from 85 patients with knee OA. They were asked to answer VAS pain scale, two disease-specific questionnaires (WOMAC and LFI) and one instrument about health-related quality(KHAQ). Reliability was assessed using cronbach's alpha. Correlation was assessed by examining the pearson's correlation coefficients. Results : Cronbach's alpha of all Index except WOMAC stiffness subscale(.6679) exceeded the value of 0.70. A good correlation was obtained between WOMAC Subscale (especially Pain Subscale) and LFI (.726<cronbach's alpha<.791). Also, Correlation between KHAQ and two disease-specific questionnaires (WOMAC and LFI) was good(.612<cronbach's alpha<.662). Conclusions : All used instruments have good internal reliability. also, There are good correlation among them. The treatment of knee OA must be focused on health related quality. For this, we should use proper assessment instruments.
류성만,최원영,이미경,이종욱 嶺南大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.12 No.-
저자들은 1992년 9월20일부터 1992년 11월 7일까지 경상북도 경산시 영남대학교 교내에 위치한 삼천지에 대한 곤충상을 조사하였다. 연구 결과 총 8目 56科 121屬 132種이 서식하고 있는 것으로 밝혀졌다. We studied insect fauna in the Samchun-impoundment during the period of from september 20, 1992 to November 7, 1992. The total number of insect taxa are composed of 132 spceies, 121 genera, 56 families, 8 orders including this examination.