Effects of Traditional Chinese Cooking Methods on Formation of Heterocyclic Aromatic Amines in Lamb Patties

Haitao Guo,Zhenyu Wang,Han Pan,Xin Li,Li Chen,Weili Rao,Yuan Gao,Dequan Zhang 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.3

Different amounts of the potent mutagenic and/or carcinogenic heterocyclic aromatic amines (HAAs) areformed in muscle-based foods under different cookingmethods. HAAs (9 varieties) in lamb patties cooked usingtraditional Chinese cooking methods (roasting, frying, panfrying,and stewing in seasonings) were investigated. Thetotal HAAs contents in roasted, fried, pan-fried, and stewedpatties were 4.39-123.15 ng/g, 3.59-43.24 ng/g, 0.71-10.05ng/g, and 51.07-120.32 ng/g, respectively. Amounts of HAAsincreased as cooking time increased. 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) was the dominantHAAs in deep roasted and fried samples, while 1-methyl-9H-pyrido [3,4-b] indole (Harman) and 9H-pyrido [3,4-b]indole (Norharman) were the main HAAs in pan-fried andstewed samples. Types and contents of HAAs formed atdifferent cooking times using different methods are unique. Stewing in seasoning generated a higher HAAs contentthan the high-temperature cooking methods roasting,frying, and pan-frying.

Comparative proteomic analysis of parasitic loranthus seeds exposed to dehydration stress

Limei Pan,Lingyun Wan,Lili He,Lisha Song,Hairong Long,Xiaowen Ji,Ni Jiang,Juan Huo,Shugen Wei,Jine Fu 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.1

Parasitic loranthus [Taxillus chinensis (DC.) Danser] is an important medicinal plant that produces recalcitrant seeds that are sensitive to dehydration. Desiccation tolerance is critical for the survival of recalcitrant seeds in low-moisture environments. Clarifying how these seeds respond to desiccation is important for long-term conservation. Thus, the viability, germination, microstructure, and antioxidant enzyme activities of dehydrated parasitic loranthus seeds were investigated. Diverse organelles were degraded or deformed during dehydration treatments. Additionally, superoxide dismutase and catalase activities gradually decreased in response to desiccation stress. A proteomic analysis involving TMT-labeling and LC–MS/ MS were performed. A total of 1479 proteins were identifed, of which 141 were diferentially expressed proteins (DEPs) at 16 and 36 h after initiating the dehydration treatments. A functional annotation based on gene ontology revealed that the DEPs were mainly localized in chloroplasts and were related to energy metabolism, responses to stimuli, and the regulation of biological processes. A KEGG pathway enrichment analysis determined that several of the identifed proteins were associated with signal transductions, photosynthesis, and glycolysis/gluconeogenesis. The results suggest that the efcient removal of excessive ROS amounts may be crucial for promoting parasitic loranthus seed germination under dehydration stress conditions. A series of candidate dehydration stress-related proteins were identifed and may be relevant for enhancing the dehydration tolerance of the recalcitrant seeds. To the best of our knowledge, this is the frst study to elucidate the possible molecular mechanisms underlying the sensitivity of recalcitrant parasitic loranthus seeds to dehydration via a proteomic analysis involving TMT-labeling.

OSI-027 alleviates rapamycin insensitivity by modulation of mTORC2/AKT/TGF-β1 and mTORC1/4E-BP1 signaling in hyperoxia-induced lung injury infant rats

Long Li,Liang Mulin,Yanling Liu,Wang Pan,Dang Hongxing 대한독성 유전단백체 학회 2021 Molecular & cellular toxicology Vol.17 No.3

Background The mechanism of long time and high-concentration oxygen treatment leading to acute lung injury (ALI) or developmental lung disease in infants is currently unclear. Here we found that compared with the effect of rapamycin, pan-mTOR1/2 inhibitor OSI-027, alleviates hyperoxia-induced lung injury (HILI) by modulation of mTORC2/AKT/TGF-β1 and mTORC1/4E-BP1 signaling in infant rats. Objective Infant rats were treated with continuous inhalation of 90% medical oxygen. Normal saline, rapamycin, or OSI-027 was intraperitoneally injected, and the status of lung injury was tested on days 3, 7, and 14. The activation of mTOR/AKT/TGFβ1 and mTORC1/4E-BP1 signaling was confirmed by immunohistochemistry and Western blot analysis in normal and hyperoxia-treated live precision-cut lung tissues. The inhibitory effect of OSI-027 extended to the active state of other proteins implicated in mTOR1/2 signaling was demonstrated in hyperoxia-induced injured lung tissues. Results Our data demonstrate that hyperoxia-induced serious lung inflammation and fibrosis. OSI-027 significantly attenuated the pathological process of HILI, inhibit the phosphorylation of the primary downstream targets of mTORC1/C2, and reduce the activation of TGF-β1 signaling. Conclusions The results suggest that mTORC2/AKT/TGF-β1 and the rapamycin-insensitive mTORC1/4E-BP1 (Thr37/46) signaling has an important effect during HILI with a potential meaning for the progress of novel anti-hyperoxia-injury strategies. Background The mechanism of long time and high-concentration oxygen treatment leading to acute lung injury (ALI) or developmental lung disease in infants is currently unclear. Here we found that compared with the effect of rapamycin, pan-mTOR1/2 inhibitor OSI-027, alleviates hyperoxia-induced lung injury (HILI) by modulation of mTORC2/AKT/TGF-β1 and mTORC1/4E-BP1 signaling in infant rats. Objective Infant rats were treated with continuous inhalation of 90% medical oxygen. Normal saline, rapamycin, or OSI-027 was intraperitoneally injected, and the status of lung injury was tested on days 3, 7, and 14. The activation of mTOR/AKT/TGFβ1 and mTORC1/4E-BP1 signaling was confirmed by immunohistochemistry and Western blot analysis in normal and hyperoxia-treated live precision-cut lung tissues. The inhibitory effect of OSI-027 extended to the active state of other proteins implicated in mTOR1/2 signaling was demonstrated in hyperoxia-induced injured lung tissues. Results Our data demonstrate that hyperoxia-induced serious lung inflammation and fibrosis. OSI-027 significantly attenuated the pathological process of HILI, inhibit the phosphorylation of the primary downstream targets of mTORC1/C2, and reduce the activation of TGF-β1 signaling. Conclusions The results suggest that mTORC2/AKT/TGF-β1 and the rapamycin-insensitive mTORC1/4E-BP1 (Thr37/46) signaling has an important effect during HILI with a potential meaning for the progress of novel anti-hyperoxia-injury strategies.

벤처기업의 아웃소싱 사례연구

강판국 한국중소기업학회 2003 기업가정신과 벤처연구 Vol.6 No.1

대동바이오텍은 본래 자동차부품의 UV 코팅을 전문으로 하는 소기업이었으나 우연히 저온에서도 원적외선이 방사되는 희귀성 약돌(pegmatite)로 만든 건강 팔찌를 만들어 주변 친지들에게 판매하게 되었다. 이를 사용해본 주변의 많은 사람들이 건강상에 좋으니 본격적으로 개발해보라는 권유에 따라 대학의 창업보육센터에 입주하였고, 지엽적 시장을 벗어나기 위하여 약돌을 미세분말하여 원단에 코팅처리한 원단을 이용하여 헬스 다이어트복을 개발하기로 결정하였다. 제품의 효능을 과학적으로 증명하여 소비자들에게 제품의 신뢰도를 높이고자 경북대학교의 물질화학연구소의 이광필 박사에게 의하였다. 연구프로젝트는 진행 내용은 다음과 같다. 1. 저온에서 원적외선이 방사되는 미세분말 코팅 섬유원단을 개발 2. 저온에서 원적외선이 방사되는 미세분말 코팅 섬유원단의 특성분석 3. 저온에서 원적외선이 방사되는 미세분말 코팅 섬유원단 헬스다이어트복 개발 4. 기능성 헬스다이어트복의 임상실험을 통한 신체에 미치는 효과측정 과학적 데이터가 갖추어져 상품의 신뢰도가 높아졌으므로 제품의 전국적 대량시장으로 확장시켜 나가기 위해서 벤처기업의 시장진입 모델 연구를 호서대학교 강판국 교수와 산학연구가 체결되어 진행 프로젝트 내용은 다음과 같다. 1. 제품의 디자인과 브랜드 'PANTAS'의 전략화 이성훈 교수의 자문하에 상표의 디자인과 브랜드를 바꾸고 상표등록 2. 매스미디어의 활용 매스컴의 벤처기업의 신개발품 신상품코너를 이용 제품 및 브랜드의 홍보 3. 마케팅 채널의 선택 1) 타켓 마케팅 전략: 헬스센터, 스포츠 센터/전문점을 이용한 판매전략 2) 다단계판매망을 통한 직접 판매채널 3) 전국적 대리점을 구축 4) 판매의 아웃소싱 벤처기업들의 대부분은 자원 및 기업 인프라의 부족이라는 태생적 문제를 여전히 가지고 있다. 이러한 문제점들에 대한 해결의 단초를 제공할 수 있는 것이 아웃소싱 전략이다. 대학의 창업보육센터에 입주한 후 대학의 신뢰도와 인적인 네트워크를 이용하게 되었고 기술적 아웃소싱을 통하여 제품의 효능에 대해 과학적 증명을 통해 신뢰도를 높일 수 있었다. 경영적 아웃소싱을 통해 제품의 브랜드화, 매스미디어를 활용한 마케팅 프로모우션, 다양한 마케팅 채널 탐색과 전략을 전개해 볼 수 있었다. Daedong Bio-Tech Co. was a small business, whose primary business being UV coating of automobile parts. Daedong started to make health bracelets made of yakdol(pegmatite) emanating far-infrared rays and sold them to close acquaintances. Those who used the bracelet found that it helped their health. Encouraged by their responses, Daedong entered a business incubator operated by Keimyung University, and started to make health diet sportswear using fabrics coated with the powder of yak-dol. To secure consumer's confidence on the product by scientifically proving the effects of the product, the research was outsourced to The Material Chemical Research Institute of Kyungpook National University. The research showed that exercising wearing the product which emanates far infrared rays helps reduce fat through heightened body metabolism and the rapid circulation of the blood. This result helped Daedong to acquire confidences of consumers and distributors. The research project was conducted as follows. First, develop a fiber that emanates far-infrared light under normal temperature. Second, analyze the characteristics of a fiber that emanates far-infrared light under normal temperature. Third, develop health sportswear using the developed fiber. Fourth, measure the health effects of the sportswear through clinical tests. Also, developing marketing strategy was outsoursed to Prof. Kang Pan Kuk of Heseo University, and the project was was conducted as follows. 1. build a design and brand strategy 2. take full advantage of free mass media, such as new products introduction columns for venture companies 3. Selection of sales channel 1) determine the target market : health center, sports center/specialy stores 2) direct sales through network-marketing company 3) build up an nationwide agency 4) outsourcing of marketing function

Crack propagation in flexural fatigue of concrete using rheological-dynamical theory

Aleksandar Pančić,Dragan D. Milašinović,Danica Goleš 사단법인 한국계산역학회 2021 Computers and Concrete, An International Journal Vol.27 No.1

The concrete fatigue analysis can be performed with the use of fracture mechanics. The fracture mechanics defines the fatigue crack propagation as the relationship of crack growth rate and stress intensity factor. In contrast to metal, the application of fracture mechanics to concrete is more complicated and therefore many authors have introduced empirical expressions using Paris law. The topic of this paper is development of a new prediction of fatigue crack propagation for concrete using rheological-dynamical analogy (RDA) and finite element method (FEM) in the frame of linear elastic fracture mechanics (LEFM). The static and cyclic fatigue three-point bending tests on notched beams are considered. Verification of the proposed approach was performed on the test results taken from the literature. The comparison between the theoretical model and experimental results indicates that the model proposed in this paper is valid to predict the crack propagation in flexural fatigue of concrete.

제6차와 제7차 교육과정의 슬기로운 생활 교과서 삽화 비교 분석 : 1학년 교과서를 중심으로 Focused on the textbook of the 1st Grade

송판섭,김정덕,문병찬,김해경 한국초등과학교육학회 2003 초등과학교육 Vol.22 No.3

This study aims to analyze illustrations in Wise Life textbooks for the 6th and the 7th elementary school curricula. The 7th textbook has been decreased in the number of illustrations comparing with the 6th one. But illustrations per each page in the textbook and each lesson have been increased in number. In the ratios to a page face, these illustrations for the 6th and 7the textbooks are mostly below 1/4 up to now. Meanwhile, the illustrations of two, 2/3 and 1/3 ratios to a page face have been increased in the 7th textbook, relatively. So, it is considered that the stupid arrangements of these illustrations for 6th textbook have been improved for 7th one. Photos of above illustrations are mostly quadrangular and circular in the 6the and the 7th textbooks. Of above photos, the quadrangular ones in the 7th textbook have been decreased about 10% comparing with ones of the 6th textbook. Although the role of motivation inducement for the illustrations of the 7th textbook is more or less improved, those ones keep below 10% so far. The illustrations of the present textbooks possess backgrounds showing urban and farming lives, in general. On the other hand, it is suggested that the contents of several life styles which are exampled as a fishery village or developing field of oceanic resource would be included in the illustrations of Wise Life textbook, in future.

부산시 대기먼지 오염원의 성분비 특성

신판세,정장표 慶星大學校 1998 論文集 Vol.19 No.2

It is necessary to establish the reasonable management plans for controlling level of suspended particulate matter that source profile characterization for the suspended particulate matter are analyzed and identified. Therefore, in this study, emission inventory for the sources emitting particulate matter in Pusan area, was made and assessed through investigation and analysis and source profiles which are considered to be important information to identify the relationship between source and receptor, were developed and arranged through the comparison of related of related source profile data. From this study, the following conclusions were obtained. 1. The concentrations of TSP in Pusan were showed higher in winter and spring than in summer and autumn and the concentration and emission quantity of TSP had a close relationships. (r=0.7693) 2. The concentration of the emission quantity of TSP from the regions of Buk-Gu and Saha-Gu was dominant and calculated as 83% of total emission quantity of TSP in Pusan and the variation of that showed less than of the other regions. 3. The larger the fraction of area occupied by building and residential house was, the higher variation of TSP concentration showed. 4. In the fact that the fractions of Al, Si, K, and Ca were showed high, while that of Com Mo, and Cd were showed low, it was exist, and it was remarkable that high fraction of Cl was calculated in transportation source profile of Pusan. 5. From the results of developed source profiles for the selected point sources, it was obtained that the composition fractions of K, Na, and Ca were showed high while those of Co, Se, Mo and Ti were calculated low. And as the scale of emission facility goes larger, the composition fractions showed higher, especially this trend was showed more in alkali and alkaline-earth metals such as K, Mg, and Ca. 6. By the kind of vehicle, speed pattern and sampling place for the source profile of transportation, and used fuel, emission facility, the kind and the scale of industry for that of point sources, it was identified that the source profiles were different from each other. 7. The related study on source profile should be performed to get the reprehensive source profiles for the sources contributed to level of suspended particulate matter in Pusan.

자석을 이용한 영재학습도구 개발 및 적용

송판섭,문병찬 光州敎育大學校 初等敎育硏究所 2003 初等敎育硏究 Vol.17 No.2

The instruction for the development of creativity of is not only the important gifted studying goal but its dealing in the seventh curriculum. For this reason, we are trying to make an effort to develop the creative programs. Many students can acquire the scientific principles by the experiment activities, which can lead them to the stage of the creative development. One of these kinds of the experiment activities, the loadstone which is the familiar with our life is interesting by most elementary student. But most of the students always perceive that there is the attraction between the loadstone and iron, and they think that two iron must need inserted the loadstone in order to addict directly each other. This is invented to change the students' preconception above this and to expand the students' curiosities on the loadstone: the equipment used magnetic field is that the students can see that two iron are contacted without the relation directly to the loadstone.

광합성의 생리생태 (2) : 환경변화에 대한 광합성의 적응반응 Adaptation of the Photosynthetic Apparatus to Changing Environment

김판기,이은주 한국농림기상학회 2001 한국농림기상학회지 Vol.3 No.3

현장실험을 통한 VES-LMC 균열발생 원인분석

최판길,이봉학 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

Many concrete bridge decks develop transverse cracking shortly after construction. These cracks accelerate corrosion of reinforcing steel and lead to concrete deterioration, damage to components beneath the deck, unsightly appearance. These cracks shorten the service life and increase maintenance costs of bridge structures. In this study, VES-LMC overlay, which provides the same benefits as a conventional overlay, is designed to cure very quickly. Although the materials for VES overlays are more expensive, the cost is more than offset by the savings on traffic control and work zone safety measures. Otherwise, reaction of hydration occurs very rapidly in beginning step(concrete placing). As a results, thermal cracking can be occur by thermal stress in accordance with hydration-heat The purpose of this study was to estimate diagnosis of crack occurrence of VES-LMC through field tests at early-age.