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- 저자 : Xiaowen Ji (검색결과 3 건)
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Comparative proteomic analysis of parasitic loranthus seeds exposed to dehydration stress
Limei Pan,Lingyun Wan,Lili He,Lisha Song,Hairong Long,Xiaowen Ji,Ni Jiang,Juan Huo,Shugen Wei,Jine Fu 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.1
Parasitic loranthus [Taxillus chinensis (DC.) Danser] is an important medicinal plant that produces recalcitrant seeds that are sensitive to dehydration. Desiccation tolerance is critical for the survival of recalcitrant seeds in low-moisture environments. Clarifying how these seeds respond to desiccation is important for long-term conservation. Thus, the viability, germination, microstructure, and antioxidant enzyme activities of dehydrated parasitic loranthus seeds were investigated. Diverse organelles were degraded or deformed during dehydration treatments. Additionally, superoxide dismutase and catalase activities gradually decreased in response to desiccation stress. A proteomic analysis involving TMT-labeling and LC–MS/ MS were performed. A total of 1479 proteins were identifed, of which 141 were diferentially expressed proteins (DEPs) at 16 and 36 h after initiating the dehydration treatments. A functional annotation based on gene ontology revealed that the DEPs were mainly localized in chloroplasts and were related to energy metabolism, responses to stimuli, and the regulation of biological processes. A KEGG pathway enrichment analysis determined that several of the identifed proteins were associated with signal transductions, photosynthesis, and glycolysis/gluconeogenesis. The results suggest that the efcient removal of excessive ROS amounts may be crucial for promoting parasitic loranthus seed germination under dehydration stress conditions. A series of candidate dehydration stress-related proteins were identifed and may be relevant for enhancing the dehydration tolerance of the recalcitrant seeds. To the best of our knowledge, this is the frst study to elucidate the possible molecular mechanisms underlying the sensitivity of recalcitrant parasitic loranthus seeds to dehydration via a proteomic analysis involving TMT-labeling.
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Wan Lingyun,Pan Limei,Song Lisha,Fu Jine,Huang Qiulan,Ji Xiaowen,Wei Shugen,Zhang Zhanjiang 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.5
The hook of Uncaria rhynchophylla is an important external phenotype representing the quality of Gou-Teng. The hook formation pattern of U. rhynchophylla displays a unique feature that solitary hook and opposite hooks alternately occur in the leaf axils forming a liner series. The solitary hook only grows on the lower side of the leaf axil, resulting from the decline in the total yield of hooks. However, the reasons for solitary hook formation in U. rhynchophylla are not clear. Therefore, a survey on the molecular mechanisms of solitary hook formation needs to be performed urgently. We obtained 250.67 million high-quality clean reads from the lower side of axillary stem segments (HS) and the upper side of axillary stem segments (HSCK) libraries. We detected 42 DEGs (differentially expressed genes) between HS and HSCK libraries; 11 were down-regulated, and 31 were up-regulated. Gene ontology functional classification of the DEGs indicated that they included two genes that encoded LAZY1 related to the regulation of polar auxin transport, two genes that encoded AUX/IAA and AP2/ERF related to plant hormone signaling transduction, and two genes that encoded F3H related to the flavone synthase activity. These six genes were associated with lateral polar auxin transport (PAT) regulation, and their expression level had a high correlation with the pattern of IAA distribution upon gravity stimulation. And the inhibition of solitary hook development was observed after treatment with N-1-naphthylphthalamic acid (NPA) or quercetin, which was known as the polar auxin inhibitor. The above findings might provide new information about the molecular mechanisms of regulation of lateral PAT in the axillary bud development process under gravity.
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Strategy to increase distortional rigidity of crane box girder: Staggered truss diaphragm
Yangzhi Ren,Wenjing Guo,Xuechun Liu,Bin Wang,Piyong Yu,Xiaowen Ji 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.86 No.4
This paper proposes a novel method for increasing the distortional frame rigidity of off-rail box girder bridges for cranes by reinforcing the diaphragm with staggered truss. The study starts by using the Matrix Displacement Method to determine the shear angle of the staggered truss diaphragm under two assumptions: hinge joint and rigid joint. To obtain closedform solutions for the transversal and longitudinal deformations and warping stress of the crane girder, the study employs the Initial Parameter Method and considers the compatibility of shear deformation at joints between the diaphragms and the girder. The theoretical solutions are validated through finite element analysis, which also confirms that the hinge-joint assumption accurately represents the shear angle of the staggered truss diaphragm in girder distortion. Additionally, the study conducts extensive parameter analyses to examine the impact of staggered truss dimensions on distortional stress and deformation. Furthermore, the study compares the distortional warping stresses of crane girders reinforced with staggered truss diaphragms and those reinforced with perforated ones, emphasizing the importance of incorporating stagger truss in diaphragms. Overall, this paper provides a thorough evaluation of the proposed approach's effectiveness in enhancing the distortional frame rigidity of off-rail box girder bridges for cranes. The findings offer valuable insights into the design and reinforcement of diaphragms using staggered truss to enhance the structural performance of crane girders.
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