RAPD와 핵 리보솜 DNA ITS2 염기서열에 의한 한국산 전나무속 식물의 유전적 변이 및 계통유전학적 분석

정용환,한상현,오유성,고미희,고석찬,김문홍,오문유 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

We analyzed the genetic variation and phylogenetic relationship among fourteen taxa of GenusAbies distributed in Korea using random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) 2 sequences of the nuclear ribosomal DNA (rDNA). RAPDs were used for identification of selected taxa by amplification using single 10-mer primers of arbitrary sequence. Fourteen taxa were clearly classified with 10 arbitrary primers, which generated 102 useful polymorphic bands that were evaluated according to the presence or absence of the fragments of equal size. The genetic distance between A. koreana for. chlorocarpa and A. koreana for. rubrocarpa was 0.127, showing the closest among any other pairs. By comparing the base sequences in the ITS2 region of the nuclear rDNA, genetic relationship was assesed among selected taxa. It showed twenty six sites of point mutation (base substitution) in 243 base pairs (bp) of ITS2 region. The G+C contents of ITS2 region were ranged from 58.3% to 61.7%. The value of nucleotide divergence among selected taxa was ranged from 0.4% to 9.8%. These results showed that RAPD and ITS2 sequence analysis was a useful tool for elucidating phylogenetic relationship and selection of specific marker in Abies species.

벗나무속 수종간의 핵 리보솜 DNA의 ITS Ⅰ 염기서열 비교

오유성,김기옥,정용환,오문유,한상현,고미희 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-

Comparison of four species (Prunus yedoensis, P. sargentii, P. pendula for. ascendens, P. serrulata var. quelpaertensis) and one cultivar of P. yedoensis were carried out by comparing their nucleotide sequences from the internal transcribed spacers (ITS) I of nuclear ribosomal DNA (rDNA). The length of the ITS I region was ranged from 229 to 236 base pairs (bp). The G+C contents of ITS I ranged from 58.5 to 60.2%. The value of nucleotide divergence across for four Prunus species and one cultivar ranged from 0.4 to 6%. The pairwise distance between P. pendula for. ascendens and P. yedoensis was 0.004 showing the lowest value among any other pairs. On the other hand, the pairwise distance betwen P. yedoensis and cultivated P. yedoensis was 0.026. The nucleotide analysis based on ITS I sequence was considerably similar to previous studies on the basis of random amplified polymorphic DNA (RAPD).

단백질 전기영동상에 의한 제주도 사철란속 4종간의 유전적 유연관계

오문유,송성희,김세재 제주대학교 방사능이용연구소 1989 연구보고 Vol.4 No.-

제주도 사철란속(Goodyera) 4종(Goodyera velutina, G. maximowicziana, G. schlechtendaliana, G. macrantha)에 대하여 SDS polyacrylamide gel electrophoresis (SDS PAGE)와 two-dimensional gel electrophoresis(2-DGE) 방법을 이용하여 유전적 유연관계를 조사 하였다. SDS PAGE 방법을 이용한 사철란속 4종간의 단백질 pattern은 서로 유사 하였다. 2-DGE 방법을 이용한 genetic distance는 G. maximowicziana와 G. schlechtendaliana가 0.5773으로서 가장 가까운 유연관계를 나타내었고, G. velutina와 G. macrantha간의 genetic distance는 0.5858로 나타났다. 이들에 비해 G. maximowiczina와 G. macrantha간의 genetic distance는 0.7378로서 가장 먼 유연 관계를 나타내었다. 이상과 같은 결과로 미루어 볼 때 사철란속 4종간의 유전적 유연관계는 이들의 생태적 특징과 거의 일치하는 것으로 사료된다. The genetic relationships among four species(Goodyera velutina, G. marimowicziana, G. shlechtendaliana, G. macrantha) in Cheju island were examined by one dimesional(1-D) and two-dimensional gel electrophoretic(2-D) methods. The protein patterns of four species analysed by 1-D were relatively similar to one another. The genetic distances among four species were estimated by analysing the protein spots obtained by 2-D. The genetic distances between G. maximorowicziana and G. shlechtendalina, G. velutina and G. macrantha, G. maximowicziana and G. macrantha 0.5773, 0.5858, 0.7378, respectively. The genetic relationships among four species estimated in this study were coincident with their ecological characteristics.

인권 보장의 현황과 과제 : 노동인권의 보장을 중심으로

오문완,김유미,도회근 울산대학교 사회과학연구소 2000 사회과학논집 Vol.10 No.1

우리나라에서 인권 보장의 현실을 생각할 때 우리는 앞으로도 많은 과제를 해결해야 한다. 특허 노동인권의 보장 문제가 되면 더욱 그러하다 그리고 문제를 해결하는 데는 기본적인 시각의 정립이 필요한데, 우선 노동인권의 성격에 대해 제대로 이해하고 있어야 한다. 또한 사회적 기본권의 구체적 권리성을 인정하는 견해에 주목할 필요가 있다. 아울러 노동인권의 보장에 관한 국제적인 움직임을 주시해야 한다. 노동시장의 변화하는 모습 역시 놓쳐서는 안된다. To consider the reality of guaranteeing human rights in Korea, there are many problems to be resolved, especially in the field of labor. Most of all we should settle our position about labor rights and the character of those. And it may be helpful to examine the opinion approving the concrete aspects of the social rights. We should not neglect to pursue the international movement of guaranteeing labor rights and the change of labor market.

濟州道 雜犬의 繁殖生理에 關한 硏究 : 發情 持續期間에 對하여

吳文儒,金永洪,玄寬宗 제주대학교 1976 논문집 Vol.8 No.-

An observation was made on the duration of estrus and the blood values during the estrous cycles in 15 mongrel bitches. The results obtianed were as follows ; 1. The length of proestrus was reckoned up the time from first appearance of blood in the vaginal smears to first acceptance of the male; varied 4 to 12 days (mean±S.D.=7.8±2.6 days). 2. The duration of estrus was determined by the acceptance test following the days between first and last acceptance; varied from 4 to 13 days (8.1±2.6 days). 3. The duration of visible bieeding out of vigina varied from 12 to 23 days (16.9±2.6days). 4. The seasonal occurrence of estrus did not exhibit. 5. During proestrus and estrus, the erythrocyte numbers increased 5-6% above those of established for beagle in anestrus. No significant variation in total leucocyte numbers was noted during the estrous cycles.

제주먹초파리(Drosophila sp ; Robusta Group)에서 난황 단백질 합성에 미치는 유충 호르몬Ⅲ(Juvenile hormone Ⅲ)의 영향

김기옥,오문유,김세재 濟州大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.5 No.1

제주먹초파리 (Drosophila sp: robusta group)에서의 난황 단백질 합성은 우화 후 2일된 female 성체의 지방체와 난소의 여포세포 (follicle cell)에서부터 합성되기 시작했다. 그리고 합성된 난황 단백질은 hemolymph로 방출된 후 선택적으로 난소로 흡수되어 완전히 성숙한 embryo를 생성하는데는 우화 후 약 4일정도가 소요되었다. 난황 단백질 유전자 발현은 곤충 호르몬인 20-hydroxyecdyson(20HE)과 juvenile hormone (JH)에 의하여 조절된다. 미성숙한 female성체에서 복부만을 분리하여 10-6M에서 10-3M의 juvenile hormoneⅢ 을 0.3 ㎕정도 주입하여 난황단백질을 합성을 유도 할수 있었다. 그리고 10-3M의 JHⅢ 처리후 2-3시간 사이에 난황 단백질의 합성을 추적할 수 있었다. 우화 후 2일된 female 성체에 수종의 호르몬 (Acetone, JHⅢ, JHⅢ-20HE)을 처리한 결과 난소의 발달 효과는 JHⅢ 처리구에서 가장 높았다. 따라서 JHⅢ는 vitellogenesis동안에 지방체와 여포세포에 있는 난황 단백질 유전자를 활성화시킴은 물론 난소의 발달에도 많은 영향을 미치는 것으로 보인다. Three yolk proteins of Drosophila sp (robusta group) begin to be synthesized in fat body and ovarian follicle cells of two female after eclosion, and selectively accumulated by the oocyte during vitellogenesis. Expression of the yolk protein genes are regulated by the insect hormone, each as 20-hydroxyecdyson and juvenile hormone. An injection of 0.3 ㎕ of 10-6 M JHⅢ in one day-old female was sufficient to promote synthesis and secretion of yolk proteins in isolated abdomens. Three yolk proteins were first detected between 2 and 3 hrs after treated of 10-3 M JHⅢ in abdomens removed from the whole body of one day-old female flies. The vitellogenin oocytes development by treatment with some hormones (acetone, JHⅢ, and JHⅢ-20HE) was highly affected in JHⅢ of 2-day old female flies after eclosion. Thus it seems like that increase juvenile hormone levels not only switches on yolk protein genes during vitellogenesis but also promotes the oocytes development.

제주도 畜犬의 犬사狀충에 관한 연구

金永洪,吳文儒 제주대학교 1973 논문집 Vol.5 No.-

1. Examining the blood of 440 dogs in Jeju Do by using the Modified Knott Method from April to December in 1973, 49 dogs were infected 11.1% positive. (In the blood examination in July, 19 dogs were infected among 90 dogs 21.1% positive) Measuring the microfilariae found in the blood examination, the length averaged 304.1μ(292-321μ) and the width 6.89μ(6.7-7.1μ). According to the result, the author could find all the microfilariae found in the blood examination of the dogs in Jeju Do, were only Dirofilaria immitis. 2. According to this study, microfilariae appeared in the priphery of the dog from April, increased rapidly in July, decreased gradually in October, and disappeared nearly in December from the periphery. 3. The maximal density of microfilariae in the periphery was happened at midnight, but the minimal density at midday. 4. Though mosquitoes (Ae togoi; 105, C.p.p.; 63, Ae. alb.;12) were collected from the vicinity of the infected dogs' houses examined under the stereoscope, no microfilariae were found.

유해화학물질의 계통분석

박성우,박유신,이진훈,유재훈,김유나,오세진,이진숙,김남이,박종신,남금문,노동석 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

다발성 골수종으로 이행한 단일형질세포종 1예

정재용,모성환,기장도,문철호,김창욱,전용준,박유환,정춘해,오윤경 조선의대 1995 The Medical Journal of Chosun University Vol.20 No.2

초등학교 고학년 학생의 장애아동에 대한 인식 조사

김보람,김호영,손유라,오진주,윤슬기,이효정,장문영 대한감각통합치료학회 2011 대한감각통합치료학회지 Vol.9 No.1

목적 : 초등학교 고학년 학생의 장애아동에 대한 인식을 알아보는 것이다. 연구방법 : 연구 대상은 김해시에 소재한 통합교육을 실시하지 않는 초등학교의 4, 5, 6학년의 비장애 아동이었다. 연구 기간은 2008년 10월 2일부터 10월 17일까지였으며, 설문지를 사용하였다. 설문지의 하위 영역은 사귐, 활 동, 학업 영역의 세 부분으로 각 10문항으로 이루어져 있으며 SPSS 12.0을 사용하여 분석하였다. 결과 : 장애아동에 대한 인식조사 결과 첫째, 성별에 따른 인식은 남학생보다 여학생의 평균 점수가 높았으나 통계 적으로 유의미한 차이는 없었다. 둘째, 학년에 따른 인식은 4학년의 평균 점수가 가장 높았고 6학년이 가장 낮았으 며 통계적으로 유의미한 차이가 있었다(F=3.546, p<0.05). 셋째, 장애인 접촉 유무에 따른 인식은 접촉 경험이 없 는 학생이 있는 학생보다 평균점수가 높았으나 통계적으로 유의미한 차이는 없었다. 결론 : 비장애학생의 장애아동에 대한 인식은 학년에 따라 차이가 있었다. 본 연구는 작업치료사들에게 장애아동에 대한 또래집단의 인식에 대한 정보를 제공함으로써 학교환경에 대한 이해를 도울 수 있을 것이다. Objective : The purpose of this study was to investigate attitude of grade-schoolers toward children with disabilities. Methods : The subjects of this study were grade-schoolers who have not received an inclusive education in Gim-hae. The study was surveyed from October second to 17th in 2008. The survey was categorized into three parts; comradeship, activity, and study. Each part of the survey consists of 10 questions. Data were analyzed by SPSS (version 12.0). Results : The results were as follows; First, the attitude of girls toward disabled students was non-significant compared to boys. Second, the order of attitude degree in grade of students was 4, 6, and 5. The attitude toward children with disabilities showed statistically significant difference according to grade(F=3.546, p<0.05). Third, the attitude of students who have experience of contact with people with disabilities was non-significant compared to non-experienced students. Conclusion : In conclusion, therefore peer groups in lower class levels showed more positive attitude toward the children with disabilities. The result will be useful information for understanding disabled children and making positive attitude. Also it is expected that occupational therapists help disabled children to participate in school life successfully using these information.