만성 호중구성 백혈병 1례

기장도,박치영,류보열,김태종,모성환,곽재정,문철호,박근홍,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

Chronic neutrophilic leukemia is a very rare myeloproliferative disorder which is characterized by severe sustained mature neutrophilic leukocytosis in peripheral blood (PB), hepatosplenomegaly, elevated leukocyte alkaline phosphatase, serum uric acid. serun viatmin B 12 and the bleeding tendency despite normal platelet count and coagulation time, absence of philadelphia chromosome, and the absence of fever or underlying infection or disease sufficient to mimic a leukemoid reaction. We have experienced a 34-years-old male patient with atypical chronic neutrophilic leukemia. On admission, the leukocyte count was 56,860/uL. severe neutrophic leukocytosis with 76% neutrophils and rare immature forms on PB smear. Many neutrophils had toxic granules, and vacuoloes. Leukocyte alkaline phosphatase score was decreased. The cytogenetic study showed Philadelphia chromosome negative with normal karyotype.

First Record and Morphological Features of the Dinoflagellate Peridinium aciculiferum Lemm. (Dinophyceae) in Korean Freshwater

기장서,한명수 한국환경생물학회 2005 환경생물 : 환경생물학회지 Vol.23 No.4

Two morphologically different species of Peridiniumwere frequently observed innatural samples collected monthly from Togyo Reservoir. One was previously identified as P.bipes Stein f. occultatum(Lindem.) Lef.(Ki and Han 2005), the other was remained uncertainly sofar. We resolved their identity, based on the morphological observations with scanning electronmicroscopy. The formula of epithecal plate was recorded with 7 precingular, 3 intercalary and 4apical plates (4′, 3a, 7′′). An apical pore was apparently present. The conspicuous morphology ofthe Peridiniumwas three spines on the hypothecal plates. The average body length and widthwere 33.6m with a range of 2645 m, and 25.7 with a range of 19 31 m, respectively; thecausative organism was identified as P. aciculiferum(Lemm.), which was reported for the firsttime in Korean freshwater.

국민투자신탁

기장서,최성수 대한설비공학회 1995 대한설비공학회 강연회 및 기타간행물 Vol.- No.-

노르웨이 북극다산기지 주변에 형성된 일시적 담수지의 미세조류 및 Chlamydomonas 18S rDNA의 유전자 특성

기장서,강성호,박범수,한명수,정승원 한국해양과학기술원 2006 Ocean and Polar Research Vol.28 No.2

Freshwater algal studies in North polar environments are relatively few. This study presented the algal-flora, -biomass and genetic features of dominant cells collected from temporary ponds around the Polar Research Station (PRS), Norway. Water samples were collected from 4 stations around PRS, and analyzed for their environmental and biological variables. Water temperature, salinity and conductivity ranged from 5 to 10oC, 0.1 to 0.3 and 0.21 to 0.36 mS/cm, respectively. Chlorophyll a concentration ranged from 1.8 to 11.1 mg/l, and that of the size-fractionated cells was recorded from 0.7 to 1.1 mg/l in picoplankton, 0.3 to 6.5 mg/l in nanoplankton, and 0.4 to 3.9 mg/l in microplankton, respectively. Algal flora in the present study was recorded as 10 genera, in which Chlamydomonas, particularly, was dominant in all studied sites. By comparison of Chlamydomonas 18S rDNA sequences, including two isolates from PRS, they formed a distinct clade against others: sequence similarity was significantly low (<97.2%) with C. noctigama, being the highest score by BLAST search in GenBank. This study was valuable for basic knowledge regarding the freshwater algae around PRS and their genetic information.

한국산 와편모조류 Peridinium bipes f. occultatum의 Small-Subunit Ribosomal DNA(SSU rDNA) 염기서열 분석

기장서,조수연,한명수,Ki, Jang-Seu,Cho, Soo-Yeon,Han, Myung-Soo 한국조류학회(藻類) 2005 ALGAE Vol.20 No.1

To clarify some confusions concerning identification of the Korean Peridinium species, genotypic analysis was performed with their SSU rDNA sequences. PCR was used to amplify the partial SSU rDNA of Peridinium isolates collected from three different Korean waters (Juam, Sang-sa and Togyo Reservoirs). The PCR products were allowed directly to sequence, which revealed each 942 bp of rDNA sequence. Analyses of the rDNA sequences showed that all the Korean isolates had the same genotype (100% sequence homology), and they were nearly identical to a Japanese strain of P. bipes f. occultatum (NIES 364; 99.8% sequence similarity). The sequence-based comparisons could clearly resolve P. bipes f. occultatum isolated from three different Korean waters.

Vibrio 속 16S rRNA 유전자 염기서열의 이질성 분석

기장서,Ki, Jang-Seu 한국미생물학회 2009 미생물학회지 Vol.45 No.4

세균 16S rRNA 유전자 염기서열은 분자계통분류, 진화역사 규명, 미생물 검출 등 다양한 목적으로 이용되어 왔다. 세균 제놈(genome)은 multiple rRNA 오페론을 갖고 있으며, 이들 유전자 염기서열은 일부 변이가 있는 것으로 알려져 있다. 본 연구에서는 Vibrio 속의 16S rRNA 유전자 염기서열을 이용하여 세포 내 16S rRNA의 이질성을 규명하였다. 분석은 GenBank 자료 중에서 제놈 염기서열 annotation이 완료된 V. cholerae, V. harveyi, V. parahaemolyticus, V. splendidus, V. vulnificus를 이용하여 실시하였다. Vibrio 속은 1번 염색체에 7~10개의 16S rRNA 유전자 copy를 갖고 있으며, 이들의 세포 내 유전자 변이는 0.9% 이하 상이성(99.1%이상 DNA 상동성)을 보였다. 2번 염색체에서는 16S rRNA 유전자가 1개 이하로 존재하였다. 유전체내 16S rRNA 유전형은 최소 5개(V. vulnificus #CMCP6)에서 최대 8개(V. parahaemolyticus #RIMD 2210633, V. harveyi #ATCC BAA-1116)로 조사되었다. 본 결과는 Vibrio 속의 16S rRNA 유전자 염기서열이 높은 이질성을 갖는 것을 제시해 준다. Bacterial 16S rRNA gene sequences have been widely used for the studies on molecular phylogeny, evolutional history, and molecular detections. Bacterial genomes have multiple rRNA operons, of which gene sequences sometimes are variable. In the present study, heterogeneity of the Vibrio 16S rRNA gene sequences were investigated. Vibrio 16S rRNA sequences were obtained from GenBank databases, considering the completion of gene annotation of Vibrio genome sequences. These included V. cholerae, V. harveyi, V. parahaemolyticus, V. splendidus, and V. vulnificus. Chromosome 1 of the studied Vibrio had 7~10 copies of the 16S rRNA gene, and their intragenomic variations were less than 0.9% dissimilarity (more than 99.1% DNA similarity). Chromosome 2 had none or single 16S rRNA gene. Intragenomic 16S rRNA genotypes were detected at least 5 types (V. vulnificus #CMCP6) to 8 types (V. parahaemolyticus #RIMD 2210633, V. harveyi #ATCC BAA-1116). These suggest that Vibrio has high heterogeneity of the 16S rRNA gene sequences.

Comparative Molecular Analysis of Freshwater Centric Diatoms with Particular Emphasis on the Nuclear Ribosomal DNA of Stephanodiscus (Bacillariophyceae)

기장서 한국조류학회I 2009 ALGAE Vol.24 No.3

DNA-based discrimination of species is a powerful way for morphologically otherwise similar species, like centric diatoms. Here, the author sequenced long-range nuclear ribosomal DNAs, spanning from the 18S to the D5 region of the 28S rDNA, of Stephanodiscus, particularly including a Korean isolate. By comparisons, high DNA similarities were detected from the rDNAs of nine Stephanodiscus (>99.4% in 18S rDNA, >98.0% in 28S rDNA). Their genetic distances, however, were significantly different (Kruskal-Wallis test, p < 0.01) compared to two related genera, namely Cyclotella and Discostella. In addition, genetic distances of 18S rDNAs were significantly different Student’s t-test, p = 0.000) against those of the 28S rDNAs according to individual genera (Cyclotella, Discostella, and Stephanodiscus). Phylogenetic analyses showed that Stephanodiscus and Discostella showed a sister taxon relationship, and their clade was separated from a cluster of Cyclotella (1.00 PP, 100% BP). This suggests that Stephanodiscus has highly conserved sequences of both 18S and 28S rDNA; however, Stephanodiscus is well-separated from other freshwater centric diatoms, such as Cyclotella and Discostella, at the generic level.

○○대학교 의과대학 부속병원 설계사례

기장서,최영범 대한설비공학회 2000 대한설비공학회 학술발표대회논문집 Vol.2000 No.-

New Record of the Freshwater Dinoflagellate Peridinium umbonatum Stein (Dinophyceae) from Togyo Reservoir, Korea [Note]

기장서,한명수 한국조류학회I 2008 ALGAE Vol.23 No.2

Cells of the dinoflagellate Peridinium were frequently observed in water samples of Togyo reservoir, and some species were responsible for dense blooms. Recently, we could identify them as P. bipes f. occultatum Lindem. and P. aciculiferum Lemm., considering morphology (Ki et al. 2005a; Ki and Han 2005b): However, some unidentified Peridinium cells with different shapes and body sizes were found among the samples collected during early spring. Here we describe their morphological characteristics such as thecal plate and body size to characterize its taxonomic identity by morphological characters. The formula of epithecal plates was recorded as 4 apical, 2 intercalary and 7 precingular plates (i.e. 4’, 2a, 7’’) and the epicone in an apical view was symmetric. An apical pore was easy to make out under a light microscope. No cingular displacement was observed. The average body size was 33 μm in length with a range of 26-36 μm, and average 26 μm in width with a range of 21-31 μm, respectively; the cell was, therefore, shown slightly elongated. This way we identified Peridinium umbonatum Stein, 1883 for the first time from Korean freshwaters.

A Versatile Method for DNA Sequencing of Unpurified PCR Products using an Automated DNA Sequencer and Tailed or Nested Primer Labeled with Near-infrared Dye

기장서,한명수 한국수산과학회 2006 Fisheries and Aquatic Sciences Vol.9 No.2