Simulated Moving Bed를 이용한 Bupivacaine 키랄분리 : (1) Preparative Chromatography를 이용한 기본조건 결정 (1) Optical Separation Conditions for Preparative Chromatography

윤지연,이중기,서성섭,최민호,박태진 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.6

Racemate Bupivacaine의 키랄분리를 위한 크로마토그래피의 최적분리조건을 조사하였다. Kromasii^(�) 키랄고정상에서 최적이동상조성 성분은 n-hexane/2-propanol/acetic acid/triethylamine, 99/1/0.3/0.05(vol.%)이였다. 이동상 조성성분인 2-propanol의 부피비가 증가할수록 체류시간과 분리도가 동시에 감소되었다. 이동상 성분 중에서 미량으로 존재하는 triethylamine이 증가할수록 피크의 sharpness가 증가하고 체류시간 역시 감소하였다. 또한, acetic acid가 없으면, 크로마토그래피 광학분할이 불가능하였으며 부피 0.3%에서 분리도가 가장 높음을 확인할 수 있었다. 한편, 키랄의약품의 대량분리방법중의 하나인 SMB(simulated moving bed)크로마토그래피 시스템(직경 1.0cm×10cm×8개 칼럼으로 구성)의 최적분리조건을 전산모사에 의해 구하였으며, 이를 실험으로 확인하였다. 그 결과 switching time 15분, feed의 유량 0.1 ml/min, desorbent 0.86 ml/min, raffinate 0.3 ml/min, extract 0.66 ml/min, recycle 1.0 ml/min일 때 100% 순도의 R-form과 S-form을 얻을 수 있었으며, 실험치와 전산모사치간의 오차는 ??2%이내였다. The chiral separation of racemate Bupivacaine was carried out to find the optimum condition for chromatographic system. The optimum species and composition for mobile phase were n-hexane/2-propanol/acetic acid/triethylamine, 99/1/0.3/0.05(vol.%) in case Kromasil� chiral stationary phase was employed. The retention time and resolution decreased with the increase in the ratio of 2-propanol in the mobile phase. The sharpness of peak and retention time decreased, as the content of triethylamine increased. Without the existence of acetic acid, the chromatographic separation didn't occur, and the resolution was the highest at the acetic acid volume ratio of 0.3%. The optimum condition of SMB (simulated moving bed) was determined by simulation and its results was compared with exprimental data from lab-scale SMB (10 mm 1D×8 ea). Rased on the SMB simulation and experimental results, 100% purity of R and S Bupivacaine were obtained and the error between the calculated and experimental value was within ±2%.

오이 추출물에 존재하는 Superoxide Dismutase의 열안정성

김은애(Eun-Ae Kim),김기남(Gi-Nahm Kim),길지은(Ji-Eun Kil),이민경(Min-Kyung Lee),김석환(Suk-Hwan Kim),서정식(Chung-Sik Suh),박인식(Inshik Park) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.27 No.6

오이속의 조효소액에 존재하는 Superoxide dismutase(SOD)활성의 pH 안정성은 pH 8.0에서 가장 안정하였고 pH5.0~9.0 사이의 범위에서는 비교적 안정하였다. 최적 온도는 25℃였고 열 안정성은 60℃까지는 안정하였다. 100℃에서 5분간 보관하였을 경우에는 12%만이 남아있었다. 오이에 존재하는 SOD 활성이 섭취 후에도 안정한가를 확인하기 위한 실험에서는 위속의 pH와 동일하도록 오이속의 조효소액의 pH를 2.0으로 변형시킨 후 36.7℃에서 3시간 동안 보관 후에 잔존활성이 10%였고, 장내의 환경인 pH 7.0으로 바꾸어 6시간 동안 둔 후 잔존하는 SOD의 활성은 25%로 활성이 증가되었다. 다양한 열처리 후에 잔존하는 오이의 SOD활성은 오이속은 데치기에서(끓는 물에서 2분) 25%, 껍질은 찌는 동안에(3분) 53%, 그리고 속과 껍질로 분리하지 않은 오이는 데치기에서 27%의 활성잔존률을 보였다. 4℃에서는 20일간 보관한 후에 오이속의 조효소액은 81%활성이 있었고, 30℃에서는 17%의 활성이 남아 있었다. 투석한 결과 SOD의 활성은 변화가 없었으므로 오이속에 존재하는 SOD는 적어도 분자량이 12,000 이상의 물질로 추정된다. The superoxide dismutase(SOD) in peeled pericarp of cucumber was most stable at pH 8.0 and relatively stabe between pH 5.0 and 9.0. The enzyme was stable up to 60℃ and retained 12% by heat treatment at 100℃ for 5 min. At pH 2.0, the peeled pericarp enzyme activity was decreased to 10% by incubation for 3 hrs. However, the enzyme activity was increased above 25% after incubating the enzyme at pH 7.0 for 6 hrs. Retention of SOD activity in cucumber by various heating methods was also measured. The residual SOD activities of peeled pericarp and whole cucumber was estimated to be 25% and 27% after blanching (2 min), respectively. The skin enzyme retained 53% of its activity after steaming (3 min). When the peeled peri carp enzyme was incubated at 4℃ for 20 days, the enzyme activity remained about 81%. However, when the enzyme incubated at 30℃ for 20 days, the peeled pericarp enzyme activity decreased to 17% of its original activity. The enzyme activity of peeled pericarp cucumber was not changed after exhaustive dialysis for 3 days, which indicated that the SOD activity in cucumber seems to have molecular weight above 12,000.

복강경 검사로 진단한 결핵성 복막염 1례

박민경,서원석,박재옥,김창휘,고은석,임철완 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Tuberculous peritonitis is discovered from sixty to ninty percent of pulmonary tuberculosis patients. Clinical symptoms are nonspecific such as mild fever, weakness and malaise but show tenderness in right lower abdomen and abdominal distension. Diagnosis is confirmed by laparoscopic and pathologic findings. We report a case of tuberculous peritonitis in an 11-year-old boy who was hospitalized because of abdominal distension with pain on right lower abdomen which had developed one day before he came to hospital. Chest X-ray showed pleural effusion on both sides. We did laparoscopic examination and observed diffuse nodular infiltration. In biopsy specimen we observed osseous granuloma and acid-fast bacilli in AFB stain.

Hop Extract Produces Antinociception by Acting on Opioid System in Mice

Park, Soo-Hyun,Sim, Yun-Beom,Kang, Yu-Jung,Kim, Sung-Su,Kim, Chea-Ha,Kim, Su-Jin,Seo, Jee-Young,Lim, Su-Min,Suh, Hong-Won The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.3

In the present study, the antinociceptive profiles of hop extract were characterized in ICR mice. Hop extract administered orally (from 25 to 100 mg/kg) showed an antinociceptive effect in a dose-dependent manner as measured in the acetic acid-induced writhing test. Antinociceptive action of hop extract was maintained at least for 60 min. Moreover, cumulative response time of nociceptive behaviors induced with intraplantar formalin injection was reduced by hop extract treatment during the 2nd phases. Furthermore, the cumulative nociceptive response time for intrathecal injection of substance P ($0.7{\mu}g$) or glutamate ($20{\mu}g$) was diminished by hop extract. Intraperitoneal pretreatment with naloxone (an opioid receptor antagonist) attenuated antinociceptive effect induced by hop extract in the writhing test. However, methysergide (a 5-HT serotonergic receptor antagonist) or yohimbine (an ${\alpha}_2$-adrenergic receptor antagonist) did not affect antinociception induced by hop extract in the writhing test. Our results suggest that hop extract shows an antinociceptive property in various pain models. Furthermore, the antinociceptive effect of hop extract may be mediated by opioidergic receptors, but not serotonergic and ${\alpha}_2$-adrenergic receptors.

이질균의 R Plasmid의 유전적 특성

서민호,서성일,박종욱 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

In order to choose adequate antimicrobial drugs for shigellosis and to know the mechanisms of durg resistances and molecular epidemiologic data, 67 strains of Shigella flexneri and 11 strains of Shigella sonnei were tested for the antimicrobial susceptibilities, conjugal transfer of R plasmids, incompatibility grouping, plasmid DNA profiles and the recombinations of resistance genes. In S. flexneri, 88.1 to 97% of the strains were susceptible to kanamycin(Km) and rifampicin(Rf), 76.1% of the strains were susceptible to nalidixic acid(Na) and 37.3 to 52.2% of strains to sulfisomidine(Su), trimethoprim(Tp), and cotrimoxazol(Co). In S. sonnei, 100% were susceptible to Km and Rf, and 63.6% to Na. There was no susceptible strain to Cm, Tc, Sm, and Su. In contrast to S. flexneri, many strains(81.8%) of S. sonnei were susceptible to Ap and few(0 to 9.1%) strains to Su, Tp, and Co. Most of drug resistances except to Na and Km in 50 to 100% of resistant strains were co-transferred to recipient E. coli RG488, indicationg that multiple drug resistances were R plamid mediated phenomenon. Most of R plasmids were 62.4 mega dalton(Mdal) in size and classified into the incompatibility group FⅡ. Somewhat larger plasmids, 115.6Mdal in size were frequently found in Shigella. Doubles containing both resistances of incoming plasmid and resident plasmid in incompatibility grouping were tested for the plasmid DNA profiles and the conjugations to the plasmid free recipient. According to these results, we fround that doubles had single recombinant plasmid, 64.5 Mdal in size and the mechanisms of the recombination of these resistance genes were thought to be transpositions between two plasmids.

Antinociceptive profiles and mechanisms of orally administered coumarin in mice.

Park, Soo-Hyun,Sim, Yun-Beom,Kang, Yu-Jung,Kim, Sung-Su,Kim, Chea-Ha,Kim, Su-Jin,Lim, Su-Min,Suh, Hong-Won Pharmaceutical Society of Japan 2013 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.36 No.6

<P>In the present study, the antinociceptive profiles of coumarin were examined in ICR mice. Coumarin administered orally (from 1 to 10 mg/kg) showed an antinociceptive effect in a dose-dependent manner as measured in the acetic acid-induced writhing test. Duration of antinociceptive action of coumarin maintained at least for 60 min. But, the cumulative response time of nociceptive behaviors induced by a subcutaneous (s.c.) formalin injection, intrathecal (i.t.) substance P (0.7 ?g) or glutamate (20 ?g) injection was not affected by coumarin. In addition, intracerebroventricular (i.c.v.) or intrathecal (i.t.) administration with coumarin (10-40 ?g) attenuated acetic acid-induced writhing response in a dose dependent manner. Intraperitoneal (i.p.) pretreatment with naloxone (an opioid receptor antagonist) attenuated antinociceptive effect induced by coumarin in the writhing test. Furthermore, i.c.v. or i.t. pretreatment with naloxone (5 ?g) reversed the decreased acetic acid-induced writhing response. However, methysergide (a 5-HT serotonergic receptor antagonist) or yohimbine (an α2-adrenergic receptor antagonist) did not affect antinociception induced by coumarin in the writhing test. Our results suggest that coumarin exerts a selective antinociceptive property in the acetic acid-induced visceral-derived pain model. Furthermore, the antinociceptive effect of coumarin may be mediated by activation of central opioid receptors, but not serotonergic and adrenergic receptors.</P>

회장에 장폐색을 유발한 Anisakiasis 1예

박수헌,서정민,문인성,백남종,심규식,김부성 대한소화기내시경학회 1990 Clinical Endoscopy Vol.10 No.2

Anisakiasis refers to the infestation of humans by species of marine nematode larvae belonging to the subfamily Anisakiae. An acute abdominal symptom may occur after ingestion of raw marine fish containing nematode larvae of the genus Anisakis. Migration of the parasite into the wall of gtomach, small intestine or, less commomly, the colon elicits a striking eosinophilic granulomatous tissue response. A 39 year old man was visited St. Vincent Hospital because of abdominal distension and tenderness. The plain abdominal X-ray showed ileus pattern suggesting intestinal obstruction. Hence we presented a case of intestinal Anisakiasis combined with intstinal obstruction.

관절염으로 발현한 재발성 다발성 연골염 1례

엄민식,박원우,서승철,이진호,조주연,최혜영,이찬희,임소덕 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Relapsing polychondritis is an uncommon multisystemic disease which is characterized by recurrent episodes of inflammation and progressive destruction of cartilaginous tissue. The cause of relapsing polychondritis is unclear and suspected to be autoimmune pathophysiology. It involves cartilage of nose, vertebra, trachea, peripheral joint as well as structures of eye, heart, blood vessels or inner ear. Auricular chondritis is present in almost all patients, and multiple arthritis, nasal chondritis, saddle nose, eye involvement, laryngotracheal involvement and skin manifestations are also present. We report a case of relapsing polychondritis presented a arthritis with review of literatures.

환자복 디자인 개선에 관한 연구

안민영;서미아;박재옥;이연희 한양대학교 2004 韓國 生活 科學 硏究 Vol.- No.24

The purpose of this project was to find out design elements of patients' uniform which satisfied needs and wants of patients and nurses. Data were obtained via a self-administered questionnaire from 127 patients who were admitted and nurses who employed to Hanyang University Hospital. The questionnaires were composed of dissatisfaction of exiting patient's uniform, evaluation criteria, design preference, and demographic factors. For data analysis, Descriptive statistics, one-way ANOVA, and Duncan test were employed. The results of this study were as follows; 1. Patients and nurses considered important design elements of neckline, sleeve length, waistband of the pants, and number of pocket. 2. There were differences in dissatisfaction of exiting patient's uniform related to role types. Patients considered factors related to movement such as comfort, easy to dress more importantly, while nurses considered factors related to affairs such as convenient for medical treatment, classification of size, and colorfastness to the laundry more importantly. 3. There was a significant difference in evaluation criteria of patients' uniform related to role types. Nurses considered more important expressive and economic criteria, while patients considered more important functional criteria than others. 4. There was a difference in deign preference of patients' uniform related to role types. Patients preferred uniform design which composed of U-neckline, sleeve of three-quarter length, pants of ankle length, elastic-band with a drawstring, and colors of pink and light green, while nurses preferred design which composed of round neckline, long sleeve length, pants of floor length, and colors of light green and light blue.

154kV용 활선 안전 경보기 개발에 관한 연구

정민규,조현광,우종수,안창회,서영석,김응태,박창기,오세득 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

In this report we developed a high voltage alarm for the safety of workers who check and repair the 154kV power line. This high voltage alarm we developed is attached on workers' helmet and alarms automatically in order to let workers know when workers work over the safety distance near 154kV power line. Until now the exact safety distance hasn't been regulated and high voltage alarm we've been using was for the range of 6∼22.9kV power line. We fabricated this high voltage alarm on the basis of electric density and actual measurement of power line. The experimental result was that high voltage alarm at 66kV power line alarmed near 90cm, at 154kV power line alarmed near 160cm from the line. Accordingly, we developed the new high voltage alarm which is operated in the range of 66∼154kV power line.