Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

일본 오사카시에 거주하는 일부 초등학생의 체중에 대한 인식 조사

김미옥 대구보건대학 2009 대구보건대학 論文集 Vol.29 No.-

응집과 흡착에 의한 음용수중 유해 중금속의 제거

옥치상,김정아,박미연 高神大學校 保健科學硏究所 1992 보건과학연구소보 Vol.2 No.-

Water added with humic acid(10㎎/L) and heavy metals(??, ??, ??, ??, ??) were investigated the advanced removal effects of heavy metals in drinking water by alum, ion exchange resin and activated carbon. Based on the results, the removal rates of heavy metals in optimum alum coagulation (5mg/L) were ??, 38.7%; ??, 35.5%; ??, 34.8%; ??, 19.7%; ??, 4.3%. And the removal effect series of heavy metals were ??, ?? for contact time 1hr in batch with ion exchange and ?? for filtration time 1hr in column packed with ion exchange, and ?? for contact time 1hr in batch with activated carbon and ??, ?? for filtration time 1hr in column packed with activated carbon. The removal of heavy metals by continuous treatment by column packed with ion exchange and activated carbon after alum coagulation were more increase than those of each treatment, was a marked increase as contact time was increased.

유방암 수술 후 조기 운동프로그램이 어깨관절기능, 통증, 림프부종 및 일상생활 수행능력에 미치는 영향

김미옥,전창순,조영자,조의영 대한간호협회 2005 대한간호 Vol.44 No.2

Purpose : The purpose of this study was to investigate the effects of an early exercise program after mastectomy and provide the early exercise program protocol for patients who had undergone mastectomy. Method : It was conducted with the experimental design similar to the Non-Equivalent control group pre-post test. The subjects were twenty-one women who were diagnosed with breast cancer(11 in the experimental group and 10 in the control group). The subjects in the experimental group participated in early exercise program for fourteen days. The exercise program consisted of deep breathing, shoulder stretching, pose correction of upper body, promotion of physical state, ROM exercise(passive, active). Data was analysed with a χ2-test(Fisher's exact test), Mann-Whitney U test, Wilcoxon test using SPSS WIN 11.0program. Result : There were significant differences at post exercise. The experimental group showed significant increases in shoulder flexion, adduction, abduction, internal rotation, external rotation and decreased in arm circumference of operative side. Also the experimental groups relief pain, had less difficulty of shoulder function of daily living(comb hair, fasten brassiere hook, reaching the ipsilateral scapula, and contralateral scapula with fingers on the operated side, spread a blanket, lift a shopping basket). Conclusion : The results of this study suggest that, after mastectomy, the early exercise program can make a significant contribution to the return of more normal shoulder function and decrease of swelling in operative side, relief pain, recovery of activities of daily living.

Kinematic Evaluation of Gait Variables in Developmental Studies

이경옥,김미애 이화여자대학교 동작과학연구소 2004 이화체육논집 Vol.7 No.-

본 연구의 목적은 유아 보행 발달 과정과 변화를 통한 발달 전이를 설명하기 위해 사용되는 몇가지 운동학적 변인들을 중심으로 각각의 변인들이 유아 보행의 발달 과정과 변화, 그리고 전이를 어떻게 설명하는지를 평가하는 것이다. 본 연구를 위한 실험은 부천 재활 공학 센터 보행 실험실에 설치된 Oxford Metrics 사의 Vicon 370 시스템(Oxford England)을 사용하였다. 이시스템의 자동 추적 방식은 신체 표시(mark)의 위치를 토대로 신체 내부에 가상 좌표계를 계산해 내고, 이를 기준으로 관절의 움직임을 측정하는 카단 앵글(cardan angle)방법을 채용하고있다.대상은 보행이 관성되는 시기라고 알려져 있는 1세~5세까지의 아동 총 24명이다. 시간 및 거리 보행 인자(분속수:steps/min, 보행장 : step length, 보행속도: speed:m/sec, 단하지 지지기 시간: single supprot:%), 보행 주기 동안의 전체 평균 각도, 보행 구간별 평균각, 보행 구간을 구분하기 위한 순간(event)에서의 각도, 최대값, 동적운동법위(dycamic range of motion),연령에 따른 각도, 연령에 따른 각속도를 변인으로 설정하였다. 보행인자를 통해서 보폭 , 보행 속도, 양하지 지지기가 증가하는 이유 즉, 어느 하지분절이, 어느 방향의 운동이 기여하여 그러한 결과가 나온 것인지에 대한 설명은 할 수 없다. 보행 주기 동안 하지 관절별, 운동면별, 구간별 평균값, 시점에서의 평균값 사이에는 차이가 있다. 최고 평균값과 최대 동적 범위의 연령별, 하지 관절별, 운동면별, 구간에 대한 정보를 통해 유아 보행시 하지 분절의 발달 순서와 분절간 보상을 알수 있다. 연령별 각도 그래프를 통해 연령에 따라 각 관절의 변화를 알 수 있고. 관절별 각도 그래프를 통해 관절 간, 내협웅을 알수 있다. 그러나 움직임이란 변화율이 이므로 각도 곡선 보다는 각속도 곡선이 설명력이 높다고 할 수 있다. 이렇듯 유아 보행 발달 변인들은 각각의 변인에 따라 설명할 수 있는 범위와 내용이 다르다. 그러므로 변인을 설정할 때 자신의 연구 문제를 잘 설명할 수 있는 변인으로 선택해야 한다.

그림 지도에서 수준별 교수-학습과 수행 평가의 관계 구성

장미옥,송언근 한국지리환경교육학회 2003 한국지리환경교육학회지 Vol.11 No.1

10명 전후의 소인수 학급에서 그림 지도의 수준별 교수-학습과 수행 평가의 관계 특징은 다음과 같다. 1) 그림 지도와 관련된 학습의 수준 차는 공간에 대한 개인의 인지 차이이다. 2) 따라서 공간 인지와 관련된 아동의 개인차를 진단하여 그 변인을 토대로 학습하는 것이 그림 지도와 관련된 수준별 교수-학습의 핵심이다. 3)이 같은 의미를 소인수 학급에 적용하면, 아동의 개인차를 고려한 개별화 교육이 그림 지도의 수준별 교수-학습에 가장 적합하다. 4) 따라서 그림 지도의 수준별 교수-학습은 아동들간의 비교가 아닌 개개인의 진단에 목적을 둔 수행 평가가 전제될 때 가능해 진다. 5) 이 같은 교육의 궁극적 목적은 실존적 존재로서 내 삶의 터전과 나와의 관계를 이해하는데 있다. Performance assessment was experienced as a necessity for an effective teaching and learning of drawing maps, in a class of around ten children. (1) Children’s difference in learning of drawing maps is reflective of individual difference in spatial cognition; (2) Correct assessment of individual difference in spatial cognition is thus essential to an effective teaching and learning of drawing maps at different levels of students’ understanding; (3) This leads to a necessity for performance assessment, an assessment of intra-personal strength and weakness instead of inter-personal comparison; (4) Different levels of understanding allow for individualized teaching and learning in a small classroom of ten children or so; and (5) An ultimate aim of education, at least in relation to geography, may be understanding of geography as an existential context for our situated self.

임상간호사의 비판적 사고성향, 간호수행능력 및 직무만족도

성미혜,엄옥봉 한국간호과학회 간호행정학회 2009 간호행정학회지 Vol.15 No.1

Purpose: This study was undertaken in order to investigate the relationships between critical thinking disposition, professional nursing competence and job satisfaction in clinical nurses. Method: A convenience sample of 221 registered nurses were obtained from one hospital in Seoul. The data were analyzed by descriptive statistics, t-test, ANOVA, Scheffe' test, Pearson correlation coefficient using SPSS WIN 14.0 program. Results: There were a statistically significant difference in the critical thinking disposition according to age, marital status, career length, and department. There were a statistically significant difference in professional nursing competence according to role, age, career length, current position, and department. There were a statistically significant difference in job satisfaction according to age, marital status, career length, and department. A significant positive correlation between critical thinking disposition and professional nursing competence, and job satisfaction was found. Conclusion: Critical thinking disposition, professional nursing competence and job satisfaction of clinical nurses are significantly related. To improve professional nursing competence and job satisfaction of clinical nurses, it need to use actively the critical thinking disposition with resonable salary. And it need to prepare the nursing education and strategy to increase the job satisfaction of clinical nurses.

목욕요법에 대한 고찰

이미림,옥치상 고신대학교 보건과학연구소 2006 보건과학연구소보 Vol.16 No.-

The purpose of this study is to discuss specifically about some kinds of bath therapy. The basics of bathes have many roles in washing skin's dirty, relaxing conglomerate muscles, lessening stresses, increasing blood circulation and metabolism etc. So many peoples take a bath with full-bath, half-bath and feet-bath etc. The full-bath means soaking totally to all of physical body excepting head in the hot water, the half bath soaking to half of physical body, and the feet-bath soaking to only feet. Although the important aspect of bath is to be returned parts of unhealth states, in aspects on convenience and easiness it is good to bathe feet-bath, half-bath and full-bath in order. But in aspects on making head cool, feet warm, that is, making lower body warm, relaxing lower body and dragging down heat of upper body (especially head), it is good to bathe half-bath, knee-bath and feet-bath in order.

기능평가를 통한 장애유아의 행동지원 실험연구 분석 : 1997~2006년 사이의 연구를 중심으로

이미애,한성희,이규옥 국립특수교육원 2007 특수교육연구 Vol.14 No.2

이 연구는 유아를 대상으로 기능평가를 통한 행동지원을 제공한 실험연구를 분석하여 현재의 전반적인 특징과 경향을 분석하고 문제행동 기능의 결과가 중재와 어떻게 연결되는지 살펴봄으로써 문제행동 중재를 위한 향후의 방향을 모색하고자 하였다. 이 연구의 분석 대상 논문은 주로 중다기초선 설계법을 사용하며 중재는 일반교실이나 가정 등의 자연스러운 환경에서 교사나 부모 등의 자연스러운 중재자에 의해서 이루어지고 있었으며 목표행동은 대상유아의 바람직한 행동보다는 부정적인 행동이 많았으며 중재자의 행동을 목표행동으로 본 연구도 5-6편이 있었다. 연구의 질적 수준에 대한 변인들 중 관찰자간 신뢰도를 제외하고는 중재충실도, 사회적 타당도, 유지, 일반화 등에 대한 파악이 부족한 것으로 나타났다. 기능평가는 주로 일반학급과 가정 등의 자연스러운 환경에서의 부모나 일반교사를 대상으로 한 인터뷰와 연구자나 가족, 교사 등에 의한 관칠 방법, 연구자에 의한 기능분석 방법이 많이 이용되었다. 기능평가 방법으로는 직접관찰과 인터뷰가 가장 많이 이용되었으며 평균 2~3가지 방법을 사용하고 있었으며 문제행동의 기능은 관심끌기가 가장 높았고 획득과 회피, 감각적 자극 순으로 나타났다. 행동의 기능에 따른 중재전략을 살펴보면 획득기능과 회피 기능을 가진 경우에는 선행사건 예방전략과 후속반응전략이 가장 많이 사용되었고 관심의 기능을 가진 경우에는 후속반응전략이, 감각적 자극을 목적으로 한 문제행동 중재에는 문제행동을 차단하는 전략이 사용되고 있었다. The purpose of this study was to analyze experimental studies that intervened young children's challenging behavior using functional assessment and to provide information and suggestions on future research. To accomplish this purpose, the elements of experimental study, the quality features of study(interobserver agreement, fidelity of intervention, social validity, maintenance and generalization of effect) and methods of functional assessment and intervention strategies were analyzed. The major findings of this study was followed. First, most of the analyzed articles used multi-baseline design, and the interventions were implemented were natural settings like classroom and home, and the interventionists were natural people like teachers and parents. Target behaviors were focused on problematic behaviors of subjects than desirable behaviors. Second, among the 5 quality features of study I chose, fidelity of intervention, social validity, maintenance and generalization of effect were identified poorly except interobserver agreement. Third, functional assessment was implemented by natural people like teachers and family members, and at the natural settings like classrooms and home routine. Interview to teachers and family members and direct observation by researchers, teachers and family members were used. The most common functional assessment methods were direct observation and interview. And 2 or 3 methods were used one study. The order of the function of problematic behavior was attention, obtain tangible, escape, and sensory stimulation. As an interventions strategies, antecedents events, alternative skills and consequent responding were applied comparatively the same rate. Based on this results, some recommendations are suggested.

영화에 표현된 타투(TATTOO)의 표현 특성과 타투(TATTOO)에 대한 인식전환에 관한 연구

이미옥;서미아 한양대학교 2005 韓國 生活 科學 硏究 Vol.25 No.1

When it comes to the expression characteristics of tattoos expressed in the movies, ‘Crisis of the Family’(2005) and ‘Wife, Organized Gangster’ (2001) tattoo in order to deviate the existing fixed idea that the boss of the organization should be a man and nominate women as the bosses and allow the highest authority, toughness and desire for achievement which are the natures of the bosses and it was known that tattooed patterns ensure the guarantee of the peace and future of their organizations. In ‘Butterfly (2003)’, the tattoos with butterfly patterns on each man and woman not only symbolize flying toward freedom and ideal about a new world but also express pathetic love. ‘Triple X (2002) tattoos ‘Triple X’ which is the code of the main character as a spy which is the sense of values and life style of the X generation on the back of his neck to boldly express infinite freedom. ‘Memento (2001)’ makes a short-period amnesiac as the main character in order to show the substance and false image of existence through human being’s instinctive senses by tattoos and clarify self-identity. It is necessary to recognize that tattoo plays the role of reinterpreting personality, sense and emotion of modern men by endowing the physical body with new meaning and value through post-modernism instead of simply reproducing the past, applying its figure and characteristics and creating new image on the basis of multi-culture and neo-naturalism accelerated by technological development and convenience and reminding us the essence of human being which is getting stagnant in material civilization. Furthermore, it is also required to develop it as item of popular culture that can splendidly express new trend and individual desire through leaping toward body art developed further and its value evaluation applicable to actual life and attempting diverse designs.