운동강도의 차이가 대장에서의 Heme oxygenase-1 발현에 미치는 영향: Oligonucleotide chip microarray analysis

최은주 ( Eun Ju Choi ),류호영 ( Ho Young Ryu ),차광석 ( Kwang Suk Cha ) 한국운동생리학회(구-한국운동과학회) 2012 운동과학 Vol.21 No.1

이 연구의 목적은 운동강도 차이에 의한 대장조직이 Heme oxygenase-1(HO-1)의 mRNA 발현에 어떠한 영향을 미치는지 규명하기 위해, Sprage-Dawley계 흰쥐를 대상으로 통제그룹(CON)과 저강도 운동그룹(LIE), 고강도 운동그룹(HIE)으로 구분하여 4주 동안 트레드밀운동 후 48시간 이후에 High through-put microarray analysis방법으로 다음과 같은 실험을 실시하였다. 본 실험은 Rat ABI oligo chip 26,857 개의 유전자 중 filtering을 통하여 12.079개 유전지를 선택하였고, 이 중 유의성 있는(p<.05) 유전자 12,072개를 선별하였으며, clustering분석 방법과 면역조직화학염색법에서도 HO-l이 운동강도에 따라 유의하게 발현하였다(p<.05). Microarray분석 후 RT-PCR로 확인한 결과 HO-I의 유전자 발현양상이 일치하는 결과를 얻었다. 이 실험의 결과로서 운동이 대장에서 HO-1 발현에 영향을 주는 것을 알 수 있었다. 운동이 대장에서의 mRNA 발현이 저강도 운동그룹(LIE)보다 고강도 운동그룹(HIE)에서 더 많이 발현되어지는 것으로 보아 고강도 운동에 의해 발생되는 산소라디칼을 HO-l의 유도를 통하여 적절히 제거함으로써 스스로 항상성을 유지하고 있는 것으로 생각되어진다. The purpose of this study is to identify how large intestine tissue effects to mRNA expression in Heme oxygenase-l (HO-1) due to differences in exercise intensity. To perform this experiment we divided Sprage-Dawley rats into 3 groups (control group (CON). low-intensity exercise group (LIE) and high-intensity exercise group (HIE)), and in 48 hours after 4 weeks treadmill exercise. the following experiment was performed with "High through-put micro array analysis methods" . 12,079 genes were chosen by filtering of the Rat ASI oligo chip 26.857 genes in this experiment Among 12.079 genes, we selected significant genes (p<.05) that were also expressed in the ways of clustering analysis and immunohistochemistry. With results of RT-PCR confirming and microarray analyzing, we derived that gene expression profiles of HO-l had been consistent. As a result of this experiment, we certainly identified that exercise effects to HQ-l expression in large intestine. mRNA expression was more expressed in high-intensity exercise group CHIE) than low-intensity exercise group (LIE), it seems that homeostasis maintains itself by properly removing oxygen radical. caused by high intensity exercise. through reduction of HO-l.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 東亞大學校 建設技術硏究所 1997 硏究論文集 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that Is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

Azithromycin으로 치료한 임신 중 쓰쓰가무시병 1예

김광석,최진욱,서호종,김기훈,박성호,서광섭,고성만,김순혜,김호정 대한감염학회 2001 감염 Vol.33 No.5

We report a case of tsutsugamushi disease in a 26 year-old pregnant woman who was treated with azithromycin. Her gestation period was 27 weeks and she admitted with fever, rash, and eschar on the right shoulder. Currently recommended medications for the treatment of scrub typhus are doxycycline or chloramphenicol. But, these drugs are class D drugs according to the U.S. Food and Drug Administration (FDA) Fetal Risk Summary, so they couldn't be used to treat pregnant women. Recently, a few case reports suggested that azithromycin, a relatively new macrolide antibiotic, was effective and safe for the treatment of scrub typhus in pregnant women. And, there is no evidence that azithromycin causes harmful effects to the developing fetus or to children. On the basis of current in vivo test that confirms the effectiveness of azithromycin, it may be the drug of choice for the treatment of scrub typhus in pregnant women and children. (Korean J Infect Dis 33:380-382, 2001)

국산 Lidocaine 알칼리화를 위한 Bicarbonate 혼합시 침전유발 최소량에 대한 고찰

이선호,김현수,곽인숙,안원식,최관호,김광민,손민제 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.39 No.5

Background : When local anesthetics for regional anesthesia is used, usually small amounts of bicar-bonate are added for rapid onset. This addition gives the mixed solution a more alkaline pH. The following result is an increased unionized form of the local anesthetic and rapid penetration of the drug into tiissue. Unfortunately, no data about adequate mixing volumes of domestic lidocaine and bicarbonate is available. Methods : We examined six mixing pairs of two kinds of 2% lidocaines and three kinds of 8.4% bicarbonates for minimum volumes of bicarbonate to cause a precipitation of 2% 20 ml lidocaine. Results : The mean volumes of bicarbonate to cause precipitation were 1.54 ml for Kwang-Myung lidocaine with Kwang-Myung bicarbonate, 2.90 ml for Kwang-Myung lidocaine with Dae-Won bicarbonate, 2.73 ml for Kwang-Myung lidocaine with Je-Il bicarbonate, 0.97 ml for Je-Il lidocaine with Kwang-Myung bicarbonate, 1.26ml for Je-Il lidocaine with Dae-Won bicarbonate and 1.39 ml for Je-Il lidocaine with Je-Il bicarbonate. Conclusions : We conclude that the Kwang-Myung lidocaine and the Je-Il lidocaine could cause precipitation when mixing with a smaller bicarbonate volume than foreign textbook recommended. (Korean J Anesthesiol 2000; 39: 726-729)

유도 전동기의 동특성 개선을 위한 퍼지 제어기 설계

최홍규,전광호,최병숙,강태은 홍익대학교 산업기술연구소 1998 産業技術 Vol.8 No.-

The conventional PI control that are used in the control loop for outer speed of the induction motor, also has difficulty dealing with the dynamic speed tracking parameter variation and the load disturbance. As a result, the motion control system operates with a poor performance in the motor's low speed range. In this dissertation a novel speed control system that implements and fuzzy logic controller(FLC) is proposed. The proposed FLC used the speed error its rate of change and the drive current as three fuzzy variables. The real command output current is chosen by the dufuzzification of the fuzzy output extracted from the fuzzy control rules. The flux and the torque of the induction motor are estimated using the dynamic model with an excellent dynamic response over a wide speed range and with the rotor flux field-oriented scheme of the decoupling characteristics. The performance of this control system is substantiated by computer simulations.

철근콘크리트 기둥과 철골보로 구성된 복합구조 접합부의 하중전달에 관한 연구

최광호 남서울대학교 2001 남서울대학교 논문집 Vol.7 No.-

The composite frame system with reinforced concrete column and steel beam has some advantages in the structural efficiency by complementing the shortcomings between the two systems. The system, however, has also a lot of problems in practical design and construction process due to the material dissimilarities. Considering these circumstances, this research is aimed at the development of the composite structural system which enables the steel beams to be connected to the R/C columns with higher structural safety and economy. Basically the proposed connection system is composed of four split tees, structural angles reinforced by stiffener, high strength steel rods, connecting plates and shear plates. The structural tests have been carried out to verify the moment transfer mechanism from beam flange to steel rods or connecting plates through the angle reinforced by siffener. The four prototype specimens have been tested until the flange of beam reached the plastic states. From the tests, no distinct material dissimilarities between concrete and steel have been detected and the stress transfer through wide flange beam - structural angle - high strength steel rod or connecting plate is very favorable.

主要作物의 微量要素에 關한 硏究 : 第1報 硼素 및 有機物이 배추 生育에 미치는 影響 1. Effects of boron and organic matter on the chinese cabbage

崔元凱,河浩成,鄭鎬權,姜鎬允 진주산업대학교 1969 論文集 Vol.3 No.-

硼素缺乏地帶의 배추圃場에서 硼素의 施用方法 및 施用量을 달리하여 얻은 收量으로 肥效을 追求하고, 또 完熟堆肥의 效果를 調査한 結果를 要約하면 다음과 같다. 1. 硼砂 土壤施用에 依한 배추의 收量은 分散分析結果 1% 水準의 高度의 有意性을 보였으며, 硼砂 10a當 1,500㎏施用의 것이 가장 많은 收量이었고, 그 以上의 硼砂施用에서는 오히려 減少했다. 2. 배추의 硼酸 葉面施用의 差異에 依한 收量差는 土壤施用 때처럼 뚜렷한 差를 볼 수 없었다. 大體로 硼酸 0.25%∼0.5%의 濃度로 葉面撒布한 것이 좋은 結果를 거두었다. 3. 完熟堆肥의 增施에 따라 배추의 收量은 增加되었다. 10a當 1,800㎏ 施用에서 最大値의 收量에 이르고 그 以上의 增施에서는 별다른 收量의 增加를 볼 수 없었다. This experiment was designed to make clear the effects of the application of Boron and organic matter on chinese cabbage growing in Boron dificient soil. The results obtained were as follows. 1. The application of Borax in increased remarkably the yield of the chinese cabbage. The yield was increased by the application of Borax in the soil. It reached maximum amounts at 1,500g per 10a, but decreased above 1,500g per 10a. 2. The various concentration of Boron on the leaf spray was no more effective than the application of Boron in soil. The adequate concentration of Boric Acid on the leaf spray was from 0.25 percent solution to 0.5 percent solution. 3. The yield of the chinese cabbage was increased by the application of the compeletely decayed compost. It reached maximum amounts at the application if 1,800㎏ per 10a, but there was no effects above 1,800㎏ per 10a.

고리 및 영광 논토양에 대한 ^137Cs의 모의 사고 침적시 토양-벼 전이계수

최용호,임광묵,박효국,최희주,이한수 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.4

고리 및 영광 원전 반경 5km 내 11곳의 논토양을 원상대로 재배상자에 채취하여 실험 온실로 운반한 후 벼의 이식 전과 생육중에 ^137Cs을 담수의 표면에 처리하였다. 이식전 처리시 ^137Cs의 토양-작물체 전이계수(m²kg^-1-dry plant)는 토양에 따라 8배 정도의 변이를 보였고 평균은 지역간 차이가 거의 없이 쌀알의 경우 1.1 x 10^-4 , 볏짚의 경우 2.7 x 10^-4 이었다. 분얼초기 처리와 수잉기 처리시 전이계수는 이식전 처리에 비해 대체로 2∼3 배 및 15∼30 배 정도 높았다. 토양 유기물 함량과 전이계수 간에는 정의 상관이 있었다. 본 실험결과에 입각하여 우리나라 논에 대한 일회성 사고침적시 적용할 수 있는 ^137Cs 전이계수의 대표치를 침적시기별로 제안하였다. Undisturbed soil blocks were taken into culture boxes from 11 paddy fields within 5 km radii of Kori and Yonggwang NPPs and carried to a greenhouse where ^137Cs was applied to the surface of standing water before rice transplanting and during its growth. Transfer factors (m²kg^-1-dry plant) of ^137Cs applied before transplanting varied with soils by a factor of about 8. The mean was 1.1 x 10^-4 for hulled rice seeds and 2.7 x 10^-4 for straw without significant regional differences. The ^137Cs applications at the early tillering stage and booting stage resulted in, on the whole, 2∼3 and 15∼30 times, respectively, higher transfer factors than the application before transplanting. A positive correlation was found between soil organic matter and transfer factor. Generic values of transfer factors were proposed for an acute accidental deposition of ^137Cs onto Korean paddy fields in different times of the year.

한반도 지진특성을 고려한 설계지진 결정에 관한 연구

최홍석,손영호,김종주,최광규 동아대학교 건설기술연구소 1999 硏究報告 Vol.22 No.2

Because of the frequent occurrence of the earthquakes In Korean Peninsula, it increases that the earthquake resistant design is necessary, and the design earthquake value should be estimated for the earthquake resistant design. Then, it is given in this study, the historical and instrumental earthquake data in Korean Peninsula, and those data are analyzed by applying the extreme probability distribution function and Weibull distribution function. In extreme probability distribution function, the modified first and the third asymptotic functions of the largest value theory are applied to obtain the return periods and probabilities of exceedance, and the least square method is used to estimate the parameter. In Weibull distribution function, the maximum likelihood estimate is used to estimate the parameter. The period-frequency spectrum, probability density spectrum, fourier spectrum and response spectrum analysis are peformed based on the measured seismic wave of Korean Peninsula, which are the basis of the design spectrum. It was investigated from the above performed results that the responses of the structures were influenced by the frequency contents of the data to a high degree.