누에의 行動半徑에 對한 系統間 差異에 관한 硏究

朴年圭,李海浜,金惠英 동국대학교 자연과학연구소 1986 자연과학연구 논문집 Vol.6 No.-

우리나라 장려 잠품종의 원종 중에서 14품종을 공시하여 주에의 행동반경에 대한 차이와 유전력 및 실용형질과의 관련성을 분석하였다. 행동반응은 유충의 각 시기에 따라서 품종간 차이가 있었으며 대체로 일본계통이 중국 계통에 비하여 민감한 경향이었고 각 품종 공히 행동반응이 가장 민감한 시기는 숙잡기였으며 다음은 개미누에였고 2령부토 5령까지는 상당히 둔한 경향이였다. 행동반경의 추정된 유전력은 유충의 시기별로 차이가 있었으며 h²(%)=52,121~88,411의 범위였으며, 행동발경과 타협질과의 관계에 있어서 행동반경과 생견 1 ㅣ 과수 및 행동반경과 최청부터 발아까지의 기간 사이에서는 "正"의 상관, 행동반경과 고치무게의 시이에서는 "負"의 상관이 인정되었다. The strain differences in larval walking behavior and their heredity in 14 varieties fo silk worm, Bombyx mori, has been investigate. The strain difference of walking response have been responsed in each larval stage and the bahavioral response of Japanse races was more sensitive than that of Chinese races, mostly. During matured silk worm larval stage the walking response was the highest and the next was the larvae hatched newly. Also very low sensitivity appeared from 2nd instar to 5th instar. Heritability of larvar walking distance were different depend on the larval instar the range of heritabilites(%) was 52.121-88.411. There was positive corelation between walking distance and period from incubation to emergence, but negative co relation between walking distance and single cocoon weight.

Pre-pregnancy risk factors for severe hyperemesis gravidarum: Korean population based cohort study

김호연 ( Ho Yeon Kim ),( Geum Joon Cho ),( So Yeon Kim ),( Kyu-min Lee ),( Ki Hoon Ahn ),( Sung Won Han ),( Soon-cheol Hong ),( Hyun Mee Ryu ),( Min-jeong Oh ),( Hai-joong Kim ),( Seung Chul Kim ) 대한산부인과학회 2021 대한산부인과학회 학술대회 Vol.107 No.-

제2형 당뇨병 환자에서의 혈중 렙틴 농도

김현리,배학연,장성종,김희중,정재용,김양수,김태균,박유환,정춘해 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.2

Objective: Leptin, the product of obesity (ob) gene, is a 16-kDa peptide hormone secreted by adipocytes and is thought to be a homeostatic signal that contributes to body weight regulation through modulating feeding behavior and/or energy expenditure. Mutations of the ob gene that led to leptin deficiency or production of a truncated inactive protein are associated with hyperphagia, hypometabolism, obesity, and noninsulindependent diabetes mellitus(NIDDM) in obese ob/ob mice. Leptin' s role in humans with obesity and NDDM is not known. Plasma leptin concentrations are shown to be elevated in obese humans. We questioned whether subjects with NIDDM have an altered regulation of serum leptin levels. Therefore, the object of this study is to evaluate the relationships between the serum leptin level and BMI, NIDDM. Method: This study were made of 70 subjects(control: 35, NIDDM:35) at Chosun University Hospital in Kwangju from March, 1998 to September, 1998. We measured the height and weight for BMI. Also, we measured leptin and C-peptide through radioimmunoassay. Result: The serum leptin concentration were not different in patients with type II diabetic and nondiabetic subjects (6.3 0.86/7.22 0.96, p=0.476) and the BMI was similar in diabetic and nondiabetic subjects(24 0.57/24 0.45, p=0.93). The leptin concentration were higher in women than in men regardless of diabetic status (diabetes: male 3.07 0.40 / female 8.20 1.18, p<0.05 control: male 4.88 1.28 / female 8.60 1.25, p<0.05). Conclusion: We concluded that the leptin concentrations were not different in diabetic and nondiabetic subjects and that the association of leptin with sexual dimorphism was similar in diabetic and nondiabetic subjects.

언어 발달 지연아의 발달지수와 후기 지능과의 관련성

김소연,정연경,정혜리,김우택,신임희,박정한,김행미,김진경 대한소아과학회 2004 小兒科 Vol.47 No.5

Prepregnancy Glucose Levels Within Normal Range and Its Impact on Obstetric Complications in Subsequent Pregnancy: A Population Cohort Study

Kim Ho Yeon,Ahn Ki Hoon,Cho Geum Joon,Hong Soon-Cheol,Oh Min-Jeong,Kim Hai-Joong 대한의학회 2023 Journal of Korean medical science Vol.38 No.35

Background: We sought to identify the influence of prepregnancy glucose levels on obstetric complications in subsequent pregnancy. Methods: Women in Republic of Korea who had given birth between January 1st, 2007 and December 31st, 2010 were enrolled. The database of the Health Insurance Review and Assessment Service and data from a national health screening program for infants and children were used. Subjects were divided into seven groups according to their fasting glucose levels. Results: 59,619 women were included for analysis, and 10.4%, 13.7%, 19.1%, 21.5%, 16.0%, 11.6%, and 7.5% women had glucose levels of < 75, 75–79, 80–84, 85–89, 90–94, 95–100 and > 100 mg/dL. Each 5 mg/dL increase in prepregnancy fasting glucose levels was associated with increased risk of gestational diabetes and macrosomia in subsequent pregnancy. Adjusted risk ratio for gestational diabetes per standard deviation prepregnancy glucose > 100 mg/dL was 2.015 (95% confidence interval, 1.649–2.462) and for macrosomia an adjusted risk ratio 1.389 (95% confidence interval, 1.147–1.682). Conclusion: Higher prepregnancy glucose level within normal range was related to gestational diabetes and macrosomia in following pregnancy. Our results may aid in the identification of women at future risk of obstetric complications and may guide to stratify women into normal and intensified care. Tweetable abstract: Higher prepregnancy glucose in normal range is associated with gestational diabetes and macrosomia.

Comparison of ¹⁸F-Labeled Fluoroalkylphosphonium Cations with ¹³N-NH₃ for PET Myocardial Perfusion Imaging

Kim, Dong-Yeon,Kim, Hyeon Sik,Reder, Sybille,Zheng, Jin Hai,Herz, Michael,Higuchi, Takahiro,Pyo, AYoung,Bom, Hee-Seung,Schwaiger, Markus,Min, Jung-Joon Society of Nuclear Medicine 2015 The Journal of nuclear medicine Vol.56 No.10

<P>Despite substantial advances in the diagnosis of cardiovascular disease, there is a need for <SUP>18</SUP>F-labeled myocardial perfusion agents for the diagnosis of ischemic heart disease because current PET tracers for myocardial perfusion imaging have a short half-life that limits their widespread clinical use in PET. Thus, <SUP>18</SUP>F-labeled fluoroalkylphosphonium derivatives (<SUP>18</SUP>F-FATPs), including (5-<SUP>18</SUP>F-fluoropentyl)triphenylphosphonium cation (<SUP>18</SUP>F-FPTP), (6-<SUP>18</SUP>F-fluorohexyl)triphenylphosphonium cation (<SUP>18</SUP>F-FHTP), and (2-(2-<SUP>18</SUP>F-fluoroethoxy)ethyl)triphenylphosphonium cation (<SUP>18</SUP>F-FETP), were synthesized. The myocardial extraction and image quality of the <SUP>18</SUP>F-FATPs were compared with those of <SUP>13</SUP>N-NH<SUB>3</SUB> in rat models. <B>Methods:</B> The first-pass extraction fraction (EF) values of the <SUP>18</SUP>F-FATPs (<SUP>18</SUP>F-FPTP, <SUP>18</SUP>F-FHTP, <SUP>18</SUP>F-FETP) and <SUP>13</SUP>N-NH<SUB>3</SUB> were measured in isolated rat hearts perfused with the Langendorff method (flow velocities, 0.5, 4.0, 8.0, and 16.0 mL/min). Normal and myocardial infarction rats were imaged with small-animal PET after intravenous injection of 37 MBq of <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3.</SUB> To determine pharmacokinetics, a region of interest was drawn around the heart, and time–activity curves of the <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3</SUB> were generated to obtain the counts per pixel per second. Defect size was analyzed on the basis of polar map images of <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3.</SUB> <B>Results:</B> The EF values of <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3</SUB> were comparable at low flow velocity (0.5 mL/min), whereas at higher flows EF values of <SUP>18</SUP>F-FATPs were significantly higher than those of <SUP>13</SUP>N-NH<SUB>3</SUB> (4.0, 8.0, and 16.0 mL/min, <I>P</I> < 0.05). Myocardium-to-liver ratios of <SUP>18</SUP>F-FPTP, <SUP>18</SUP>F-FHTP, <SUP>18</SUP>F-FETP, and <SUP>13</SUP>N-NH<SUB>3</SUB> were 2.10 ± 0.30, 4.36 ± 0.20, 3.88 ± 1.03, and 0.70 ± 0.09, respectively, 10 min after injection, whereas myocardium-to-lung ratios were 5.00 ± 0.25, 4.33 ± 0.20, 7.98 ± 1.23, and 2.26 ± 0.14, respectively. Although <SUP>18</SUP>F-FATPs and <SUP>13</SUP>N-NH<SUB>3</SUB> sharply delineated myocardial perfusion defects, defect size on the <SUP>13</SUP>N-NH<SUB>3</SUB> images was significantly smaller than on the <SUP>18</SUP>F-FATP images soon after tracer injection (0–10 min, <I>P</I> = 0.027). <B>Conclusion:</B> <SUP>18</SUP>F-FATPs exhibit higher EF values and more rapid clearance from the liver and lung than <SUP>13</SUP>N-NH<SUB>3</SUB> in normal rats, which led to excellent image quality in a rat model of coronary occlusion. Therefore, <SUP>18</SUP>F-FATPs are promising new PET radiopharmaceuticals for myocardial perfusion imaging.</P>

Hypoxia‐inducible transgelin 2 selects epithelial‐to‐mesenchymal transition and γ‐radiation‐resistant subtypes by focal adhesion kinase‐associated insulin‐like growth factor 1 receptor activation in non&#

Kim, In‐,Gyu,Lee, Jei‐,Ha,Kim, Seo‐,Yeon,Hwang, Hai‐,Min,Kim, Tae‐,Rim,Cho, Eun‐,Wie John Wiley and Sons Inc. 2018 Cancer Science Vol.109 No.11

<P>Microenvironment, such as hypoxia common to cancer, plays a critical role in the epithelial‐to‐mesenchymal transition (EMT) program, which is a major route of cancer metastasis and confers γ‐radiation resistance to cells. Herein, we showed that transgelin 2 (TAGLN2), an actin‐binding protein, is significantly induced in hypoxic lung cancer cells and that Snail1 is simultaneously increased, which induces EMT by downregulating <I>E‐cadherin</I> expression. Forced <I>TAGLN2</I> expression induced severe cell death; however, a small population of cells surviving after forced <I>TAGLN2</I> overexpression showed γ‐radiation resistance, which might promote tumor relapse and recurrence. These surviving cells showed high metastatic activity with an increase of EMT markers including Snail1. In these cells, TAGLN2 activated the insulin‐like growth factor 1 receptor β (IGF1Rβ)/PI3K/AKT pathway by recruitment of focal adhesion kinase to the IGF1R signaling complex. Activation of the IGF1Rβ/PI3K/AKT pathway also induced inactivation of glycogen synthase kinase 3β (GSK3β), which is involved in Snail1 stabilization. Therefore, both the IGF1Rβ inhibitor (AG1024) and the PI3K inhibitor (LY294002) or AKT inactivation with MK2206 lower the cellular level of Snail1. Involvement of GSK3β was also confirmed by treatment with lithium chloride, the inducer of GSK3β phosphorylation, or MG132, the 26S proteasomal inhibitor, which also stabilized Snail1. In conclusion, the present study provides important evidence that hypoxia‐inducible TAGLN2 is involved in the selection of cancer cells with enhanced EMT properties to overcome the detrimental environment of cancer cells.</P>

An injectable cationic hydrogel electrostatically interacted with BMP2 to enhance <i>in vivo</i> osteogenic differentiation of human turbinate mesenchymal stem cells

Kim, Mal Geum,Kang, Tae Woong,Park, Joon Yeong,Park, Seung Hun,Ji, Yun Bae,Ju, Hyeon Jin,Kwon, Doo Yeon,Kim, Young Sik,Kim, Sung Won,Lee, Bong,Choi, Hak Soo,Lee, Hai Bang,Kim, Jae Ho,Lee, Bun Yeoul,Mi Elsevier 2019 Materials science & engineering. C, Materials for Vol.103 No.-

<P><B>Abstract</B></P> <P>We have designed and characterized an injectable, electrostatically bonded, <I>in situ</I>–forming hydrogel system consisting of a cationic polyelectrolyte [(methoxy)polyethylene glycol-<I>b</I>-(poly(ε-caprolactone)-<I>ran</I>-poly(L-lactic acid)] (MP) copolymer derivatized with an amine group (MP-NH<SUB>2</SUB>) and anionic BMP2. To the best of our knowledge, there have been hardly any studies that have investigated electrostatically bonded, <I>in situ</I>–forming hydrogel systems consisting of MP-NH<SUB>2</SUB> and BMP2, with respect to how they promote <I>in vivo</I> osteogenic differentiation of human turbinate mesenchymal stem cells (hTMSCs). Injectable formulations almost immediately formed an electrostatically loaded hydrogel depot containing BMP2, upon injection into mice. The hydrogel features and stability of BMP2 inside the hydrogel were significantly affected by the electrostatic attraction between BMP2 and MP-NH<SUB>2</SUB>. Additionally, the time BMP2 spent inside the hydrogel depot was prolonged <I>in vivo</I>, as evidenced by <I>in vivo</I> near-infrared fluorescence imaging. Biocompatibility was demonstrated by the fact that hTMSCs survived <I>in vivo</I>, even after 8 weeks and even though relatively few macrophages were in the hydrogel depot. The osteogenic capacity of the electrostatically loaded hydrogel implants containing BMP2 was higher than that of a hydrogel that was simply loaded with BMP2, as evidenced by Alizarin Red S, von Kossa, and hematoxylin and eosin staining as well as osteonectin, osteopontin, osteocalcin, and type 1α collagen mRNA expression. The results confirmed that our injectable, <I>in situ</I>–forming hydrogel system, electrostatically loaded with BMP2, can enhance <I>in vivo</I> osteogenic differentiation of hTMSCs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An injectable, electrostatically bonded, <I>in situ</I>–forming hydrogel system consisting of a cationic polyelectrolyte copolymer derivatized with an amine group and anionic BMP2 was prepared. </LI> <LI> The hydrogel features and stability of BMP2 inside the hydrogel were significantly affected by the electrostatic attraction between BMP2 and cationic polyelectrolyte copolymer. </LI> <LI> The electrostatically loaded hydrogel enhanced osteogenic differentiation of human turbinate mesenchymal stem cells better than one with simple loading of BMP2. </LI> </UL> </P>

Clinical Manifestations and Genotype of Primary Ciliary Dyskinesia Diagnosed in Korea: Multicenter Study

Kim Minji,Lee Mi-Hee,Hong Soo-Jong,Yu Jinho,Cho Joongbum,서동인,Kim Hyung Young,Kim Hye-Young,Jung Sungsu,Lee Eun,Lee Sooyoung,Jeong Kyunguk,Shim Jung Yeon,Kim Jeong Hee,Chung Hai Lee,Jang Yoon Young,Kwo 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.6

Purpose: Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder that leads to secondary ciliary dysfunction. PCD is a rare disease, and data on it are limited in Korea. This study systematically evaluated the clinical symptoms, diagnostic characteristics, and treatment modalities of pediatric PCD in Korea. Methods: This Korean nationwide, multicenter study, conducted between January 2000 and August 2022, reviewed the medical records of pediatric patients diagnosed with PCD. Prospective studies have been added to determine whether additional genetic testing is warranted in some patients. Results: Overall, 41 patients were diagnosed with PCD in 15 medical institutions. The mean age at diagnosis was 11.8 ± 5.4 years (range: 0.5 months-18.9 years). Most patients (40/41) were born full term, 15 (36.6%) had neonatal respiratory symptoms, and 12 (29.3%) had a history of admission to the neonatal intensive care unit. The most common complaint (58.5%) was chronic nasal symptoms. Thirty-three patients were diagnosed with transmission electron microscopy (TEM) and 12 patients by genetic studies. TEM mostly identified outer dynein arm defects (alone or combined with inner dynein arm defects, n = 17). The genes with the highest mutation rates were DNAH5 (3 cases) and DNAAF1 (3 cases). Rare genotypes (RPGR, HYDIN, NME5) were found as well. Chest computed tomography revealed bronchiectasis in 33 out of 41 patients. Among them, 15 patients had a PrImary CiliAry DyskinesiA Rule score of over 5 points. Conclusions: To our knowledge, this is the first multicenter study to report the clinical characteristics, diagnostic methods, and genotypes of PCD in Korea. These results can be used as basic data for further PCD research.

Anti-Diabetic Effect of Pectinase-Processed Ginseng Radix (GINST) in High Fat Diet-Fed ICR Mice

Hai Dan Yuan,Hai Yan Quan,Mi Song Jung,Su Jung Kim,Bo Huang,Do Yeon Kim,Sung Hyun Chung 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3

In the present study, we investigate anti-diabetic effect of pectinase-processed ginseng radix (GINST) in high fat diet-fed ICR mice. The ICR mice were divided into three groups: regular diet group, high fat diet control group (HFD), and GINST-treated group. To induce hyperglycemia, mice were fed a high fat diet for 10 weeks, and mice were administered with 300 mg/kg of GINST once a day for 5 weeks. Oral glucose tolerance test revealed that GINST improved glucose tolerance after glucose challenge. Compared to the HFD control group, fasting blood glucose and insulin levels were decreased by 57.8% (p<0.05) and 30.9% (p<0.01) in GINST-treated group, respectively. With decreased plasma glucose and insulin levels, the insulin resistance index of the GINST-treated group was reduced by 68.1% (p<0.01) compared to the HFD control group. Pancreas of GINST-treated mice preserved a morphological integrity of islets and consequently having more insulin contents. In addition, GINST up-regulated the levels of phosphorylated AMP-activated protein kinase (AMPK) and its target molecule, glucose transporter 4 (GLUT4) protein expression in the skeletal muscle. Our results suggest that GINST ameliorates a hyperglycemia through activation of AMPK/GLUT4 signaling pathway, and has a therapeutic potential for type 2 diabetes.