白鼠 肝障碍에 對한 補肝湯 瀉肝湯의 效果에 關한 實驗的 硏究

洪茂昌,李學仁,金完熙 慶熙大學校韓醫科大學韓醫學硏究所 1983 慶熙韓醫大論文集 Vol.6 No.-

암세포 증식에 대한 YB-1 안티센스 올리고핵산염의 영향

김명성 ( Myung Sung Kim ),이완식 ( Wan Sik Lee ),박창환 ( Chang Hwan Park ),주영은 ( Young Eun Joo ),김현수 ( Hyun Soo Kim ),최성규 ( Sung Kyu Choi ),유종선 ( Jong Sun Rew ),정영도 ( Young Do Jung ),김세종 ( Sei Jong Kim ),안봉환 대한내과학회 2006 대한내과학회지 Vol.71 No.3

목적: YB-1은 PCNA, DNA 중합효소 및 MDR 유전자 등의 전사인자로 작용한다. YB-1 유전자는 정상 어른의 간에서는 발현되지 않지만 태아의 간이나 재생 중인 간에서는 발현이 현저히 증가되어서 세포의 증식과 밀접한 관련이 있음이 보고되었다. 본 연구에서는 YB-1 유전자의 안티센스 올리고핵산염을 이용하여 YB-1의 발현을 억제함으로써 암세포 증식을 차단 할 수 있는지를 실험하고 암치료를 위한 유전자 요법으로서의 가능성을 알아보았다. 방법: 세포주로는 Chang liver, HepG2, CT-26 세포를 사용하였고, 사람의 정상세포로는 섬유아세포와 내피세포가 혼재된 조직을 사용하였다. YB-1 유전자의 안티센스 올리고핵산염으로는 YB-1 cDNA의 변역 시작 부위에 상보적인 21mer 올리고핵산염을 제작하여 사용하였다. 세포의 성장은 MTT assay를 이용하였고, 유전자 발현은 Northern blot으로 분석하였으며, 세포주기 변화는 propidium iodide로 염색하여 유식세포분석기로 분석하였다. 동물실험에서는 CT-26 세포를 1.0×10(5)개씩 Balb/c 생쥐의 피하에 접종하여 종양을 유도하였다. 종양이 유도된 Balb/c 생쥐에 YB-1 안티센스 올리고핵산염을 꼬리정맥이나 종양조직에 주사한 후 종양의 크기를 측정하여 종양억제 효과를 관찰하였다. 결과: YB-1 안티센스 올리고핵산염은 CT-26 세포에서는 50 nM 이상 농도에서 Chang liver와 HepG2 세포에서는 10 nM 이상 농도에서 세포주의 성장을 강하게 억제하였지만 정상 조직세포의 성장에는 아무런 영향을 미치지 않았다. 안티센스 올리고핵산염을 DOTAP에 담지한 경우 세포성장 억제에 미치는 효과가 안티센스 올리고핵산염 단독 처치 보다 강하였다. 이때 YB-1의 발현은 증식이 억제된 세포주(Chang liver 및 CT-26)에서는 감소하였으나 정상조직세포에서는 변화가 없었다. 증식이 억제된 세포주에서 세포주기를 살펴보면 초기에 S phase가 감소함을 관찰 할 수 있었다. 꼬리정맥이나 종양조직에 YB-1 안티센스 올리고핵산염을 주입 시 종양의 크기가 유의하게 감소하였다. 결론: 이상의 실험 결과 YB-1 안티센스 올리고핵산염은 암세포의 성장을 저해하며 종양 동물모델에서 종양의 성장을 억제할 수 있음을 시사하였다. Background: Human YB-1 is a transcription factor that binds to the inverted CCAAT box in the promoter region of a variety of genes such as PCNA, DNA polymerase and MDR. In this study we evaluated the effect of YB-1 antisense oligonucleotides on tumor cell growth. Methods: Chang liver, HepG2 and CT-26 cells were cultured as immortalized cell lines. The MTT (3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyltetrazolium bromide) assay, Northern blot and flow cytometric analyses were used to determine cell growth, gene expression and cell cycle changes. In an animal model, CT-26 cells were injected into Balb/c mice to induce tumor; YB-1 antisense oligonucleotides were injected into the tail vein or tumor tissue of the mice; change of tumor size was then measured. Results: Phosphorothioated YB-1 antisense oligonucleotides suppressed the proliferation of the immortalized liver cells (Chang liver cells) and a variety of cancer cells (HepG2 and CT-26 cells); however, it did not inhibit normal cell growth. The DOTAP/antisense oligonucleotide mixture showed stronger effects on cell proliferation than did the antisense oligonucleotide alone. The YB-1 antisense oligonucleotide decreased specific expression of the YB-1 mRNA in the immortalized cancer cell lines. Flow cytometric analysis revealed that the inhibition of cell proliferation might have been due to a decrease in the S phase of the cell cycle. We found that in an animal tumor model, the administration of the YB-1 antisense oligonucleotide, in the vein or tumor tissues, decreased the tumor size significantly. Conclusions: These results suggest that the YB-1 antisense oligonucleotide may inhibit growth of a variety of cancer cells.(Korean J Med 71:293-301, 2006)

정체경락(整體經絡)마사지가 슬내,외반 하지 변형의 교정에 미치는 영향

김송자 ( Song Ja Kim ),김우원 ( Woo Won Kim ),장완성 ( Wan Song Chang ) 한국정체경락학회(구 정체경락연구학회) 2009 한국정체경락학회지 Vol.1 No.1

본 연구는 정체경락마사지와 운동요법이 슬내반과 슬외반 하지변형의 교정에 미치는 영향을 알아보고자 하였다. 특이한 지병이 없이 후천적인 하지 변형이 있는 22세-59세까지의 여성 45명을 대상으로 슬내반집단, 슬외반집단 그리고 대조집단으로 나누어 정체경락마사지와 운동요법을 8주간 주 3회씩 총 24회를 실시하였다. 각 집단의 Q각과 고관절각, 대퇴경골각의 변화를 분석하기 위하여 평균과 표준편차를 구하였으며, 처치에 따른 평균차이를 알아보기 위하여 Two-way repeated measured ANOVA, paired t-test를 실시하였다. 그 결과 슬내·외반 집단의 하지변형에 정체경락마사지 적용 전·후의 Q각에서는 우측 모두 유의한 차가 나타나지 않았으며, 좌측에서는 측정시기에서만 유의한 차(p<.05)가 나타났다. 슬내·외반 집단의 하지변형에 정체경락마사지 적용 전·후의 고관절각에서는 좌·우측에서 집단간의 유의한 차(p<.05, p<.01)이가 나타났으며, 우측에서는 상호작용효과에서도 유의한 차(p<.01)가 나타났다. 슬내·외반 집단의 하지변형에 정체경락마사지로 관리 전·후의 대퇴·경골각의 변화에서는 좌·우에서 집단 간, 측정시기, 상호작용에서 각각 통계적으로 유의한 차(p<.05), (p<.01, p<.001)가 나타났다. 무릎간의 간격과 발목간의 간격에서도 통계적으로 유의한 차(p<.001)가 나타났다. 결론적으로 정체경락마사지는 하지변형 변인, 그리고 고관절 주변 골반의 변형에 영향을 미침으로 슬내·외반 하지변의 교정에 효과가 있는 것으로 판단된다. In this study, Jung-che massage on meridian pathways and exercise therapy, Genu varus group and Genu valgus group do not want to sort out the impact was. Subjects without the disease between 20-50 women 45 men Genu varus group, Genu valgus group, and Control group was divided into the experiment. Experiments A and B, 3 times a week, 8 weeks 24 anniversary was applied. Research subjects to analyze changes in the dependent variable, mean and standard deviation were saving. Learn the difference between the treatment based on the average to Two-way ANOVA, paired t-test was conducted. As a result, There was significant difference (p<.05, p<.01) between groups at the right and left side in coxa angle before and after treatment by Jung-che massage on meridian pathways in the change of low limb of genu varus & valgus group, and also significant difference was seen in the interactive effect at the right side. For the change of low limb in genu varus & valgus group, there was significant difference (p<.05), (p<.01, p<.001) between groups at the right and left sides, measuring periods, interactions, in terms of statistics respectively as the result of looking into changes on thigh tibia angle before and after the treatment by Jung-che massage on meridian pathways. The gap between knees, which becomes the standard of judging its shape for the change on low limb of the genu varus & valgus and means genu varus, showed significant difference (p<.001) in terms of statistics. The gap between ankles, which shows genu valgus and the standard of judging low limb change of genu varus & valgus group in shape, showed significant difference (p<001) in terms of statistics. In conclusion, Jung-che massage on meridian pathway is applied to look like their give change.

Causes and Predictive Factors Associated with “Diagnosis Changed” Outcomes in Patients Notified as Tuberculosis Cases in a Private Tertiary Hospital

( Byung Ju Kang ),( Kyung Wook Jo ),( Tai Sun Park ),( Jung Wan Yoo ),( Sei Won Lee ),( Chang Min Choi ),( Yeon Mok Oh ),( Sang Do Lee ),( Woo Sung Kim ),( Dong Soon Kim ),( Tae Sun Shim ) 대한결핵 및 호흡기학회 2013 Tuberculosis and Respiratory Diseases Vol.75 No.6

Background: The aim of our study was to evaluate the “diagnosis changed” rate in patients notified as tuberculosis (TB) on the Korean TB surveillance system (KTBS). Methods: A total of 1,273 patients notified as TB cases on the KTBS in one private tertiary hospital in 2011 were enrolled in the present study. Patients were classified into three groups: “diagnosis maintained”, “diagnosis changed” (initially notified as TB, but ultimately diagnosed as non-TB), and “administrative error” (notified as TB due to administrative errors). Results: Excluding 17 patients in the “administrative error” group, the “diagnosis maintained” and “diagnosis changed” groups included 1,097 (87.3%) and 159 patients (12.7%), respectively. Common causes of “diagnosis changed” were nontuberculous mycobacterial (NTM) disease (51.7%, 61/118), and pneumonia (17.8%) in cases notified as pulmonary TB, and meningitis (19.5%, 8/41) and Crohn`s disease (12.2%) in cases notified as extrapulmonary TB. Being older than 35 years of age (odds ratio [OR], 2.18) and a positive acid-fast bacilli stain (OR, 1.58) were positive predictors and a TB-related radiological finding (OR, 0.42) was a negative predictor for a “diagnosis changed” result via multivariate logistic regression analysis in pulmonary TB cases. Conclusion: Because of a high “diagnosis changed” rate in TB notifications to the KTBS, the TB incidence rate measured by the KTBS may be overestimated. Considering the worldwide trend toward increased NTM disease, the “diagnosis changed” rate may increase over time. Thus, when reporting the annual TB notification rate in Korea, the exclusion of “diagnosis changed” cases is desirable.

Causes and Predictive Factors Associated with "Diagnosis Changed" Outcomes in Patients Notified as Tuberculosis Cases in a Private Tertiary Hospital

Kang, Byung Ju,Jo, Kyung-Wook,Park, Tai Sun,Yoo, Jung-Wan,Lee, Sei Won,Choi, Chang-Min,Oh, Yeon-Mok,Lee, Sang-Do,Kim, Woo Sung,Kim, Dong Soon,Shim, Tae Sun The Korean Academy of Tuberculosis and Respiratory 2013 Tuberculosis and Respiratory Diseases Vol.75 No.6

Background: The aim of our study was to evaluate the "diagnosis changed" rate in patients notified as tuberculosis (TB) on the Korean TB surveillance system (KTBS). Methods: A total of 1,273 patients notified as TB cases on the KTBS in one private tertiary hospital in 2011 were enrolled in the present study. Patients were classified into three groups: "diagnosis maintained", "diagnosis changed" (initially notified as TB, but ultimately diagnosed as non-TB), and "administrative error" (notified as TB due to administrative errors). Results: Excluding 17 patients in the "administrative error" group, the "diagnosis maintained" and "diagnosis changed" groups included 1,097 (87.3%) and 159 patients (12.7%), respectively. Common causes of "diagnosis changed" were nontuberculous mycobacterial (NTM) disease (51.7%, 61/118), and pneumonia (17.8%) in cases notified as pulmonary TB, and meningitis (19.5%, 8/41) and Crohn's disease (12.2%) in cases notified as extrapulmonary TB. Being older than 35 years of age (odds ratio [OR], 2.18) and a positive acid-fast bacilli stain (OR, 1.58) were positive predictors and a TB-related radiological finding (OR, 0.42) was a negative predictor for a "diagnosis changed" result via multivariate logistic regression analysis in pulmonary TB cases. Conclusion: Because of a high "diagnosis changed" rate in TB notifications to the KTBS, the TB incidence rate measured by the KTBS may be overestimated. Considering the worldwide trend toward increased NTM disease, the "diagnosis changed" rate may increase over time. Thus, when reporting the annual TB notification rate in Korea, the exclusion of "diagnosis changed" cases is desirable.

Altered Regulation of Renal Acid Base Transporters in Response to Ammonium Chloride Loading in Rats

Kim, Eun-Young,Choi, Joon-Seok,Lee, Ko-Eun,Kim, Chang-Seong,Bae, Eun-Hui,Ma, Seong-Kwon,Kim, Suhn-Hee,Lee, Jong-Un,Kim, Soo-Wan The Korean Society of Pharmacology 2012 The Korean Journal of Physiology & Pharmacology Vol.16 No.2

The role of the kidney in combating metabolic acidosis has been a subject of considerable interest for many years. The present study was aimed to determine whether there is an altered regulation of renal acid base transporters in acute and chronic acid loading. Male Sprague-Dawley rats were used. Metabolic acidosis was induced by administration of $NH_4Cl$ for 2 days (acute) and for 7days (chronic). The serum and urinary pH and bicarbonate were measured. The protein expression of renal acid base transporters [type 3 $Na^+/H^+$ exchanger (NHE3), type 1 $Na^+/{HCO_3}^-$ cotransporter (NBC1), Na-$K^+$ ATPase, $H^+$-ATPase, anion exchanger-1 (AE-1)] was measured by semiquantitative immunoblotting. Serum bicarbonate and pH were decreased in acute acid loading rats compared with controls. Accordingly, urinary pH decreased. The protein expression of NHE3, $H^+$-ATPase, AE-1 and NBC1 was not changed. In chronic acid loading rats, serum bicarbonate and pH were not changed, while urinary pH was decreased compared with controls. The protein expression of NHE3, $H^+$-ATPase was increased in the renal cortex of chronic acid loading rats. These results suggest that unaltered expression of acid transporters combined with acute acid loading may contribute to the development of acidosis. The subsequent increased expression of NHE3, $H^+$-ATPase in the kidney may play a role in promoting acid excretion in the later stage of acid loading, which counteract the development of metabolic acidosis.

위저선 폴립의 임상 및 병리학적 분석 : 가족성 선종성 폴립증에 동반된 다발성 위저선 폴립과 산발적으로 발생된 위저선 폴립의 비교 A Clinical and Pathologic Analysis with Special Reference to Familial Adenomatous Polyposis

김나영,이동호,송인성,김용일,김우호,최규완,이국래,최기영,장미수 대한소화기내시경학회 1997 Clinical Endoscopy Vol.17 No.2

Background/Aims: The aims of this study are to clarify the morphology of fundic gland polyp (FGP) and to compare the features of FGP between familial adenomatous polyposis-associated group and sporadic development group. Methods: A total of 15 endo- scopic biopsy specimens of FGP from 13 patients were divided into three groups; Group A(3 cases; familial adenomatous polyposis family, multiple FGPs), Group B(3 cases; sporadic development, multiple FGPs) and Group C(7 cases; sporadic development, single FGP), and their endoseopic /microscopic features including mucin histochemistry and immunohistoc- hemistty(for PCNA) were compared. Results: FGPs were confined to the gastric body and fundus in all 3 groups, and measured 2-8 mm. Their numbers varied even in Group A and Group B, The difference was observed in their median age: 26 years in Group A and 55 years in Group B, respectively, but there were no differences in endoscopic, histologic, mucin histochemical and immunohistochemical(for PCNA) features. Micro-scopically, all FGPs were composed of fundic glands and scattered microcysts with a spectrum of disordered glandular architecture which ranged from convoluted gland to Y-shaped gland, to stellateshaped gland, and to irregular tortuous glancl with dilated lumen. Conclusions: We assume that diversity af morphologic features of FGP may develop from progression of hyperplastic/hamartomatous fundic glandular proliferation which may end up with microcyst formation as an evolutional change. Familial adenomatous polyosis-associated FGPs were not endoscopically and histologically distingishable from sporadic deveoped FGPs.

레슬링 스타일에 따른 부상유형에 관한 연구

김정현,김창환,김병완 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.4

This study aims to investigate what types of injury frequently occur when wrestlers perform their exercise and what cause their injury and propose an efficient scheme to prevent injury. The subjects consist of 59 Greco-Roman and 53 Free-style wrestlers. The results are as follows: (1) Locations of Sports Injury In the case of Greco-Roman wrestlers, the most frequent locations of injury were in the following order: the knee joints, the wrist joints, followed by the neck, the ankle joints, the hands, the shoulder, the head, the elbow joints, the face, the breastbone, ribs, the waist, hip joints, the upper arms, the abdomen, the head, the hip, the forearms, the femoral region and trunk. In the case of Free-style wrestlers, they were in the following order: the head, the wrist joints, the face, the knee joints, the neck, the waist, the elbow joints, the ankle joints, the breastbone, ribs, the hands, hip joints, the forearms, the shoulder, the abdomen, the upper arms and trunk. (2) Types of Sports Injury In the case of Greco-Roman wrestlers, the most frequent injury types were in the following order: scratch, muscle contusion(96.6%), muscle stiffness(94.9%), muscle sprain(93.2%), sprain(91.5%), fracture(88.1%), aural hematoma(86.4%), fissure(84.7%), bone contusion(83%), incised wound or cut, collateral ligament tension and fracture(79.6%), joint sprain(76.2%), tendon fracture(64.4%), dislocation(59.3%), arthritis, disc(57.6%), cerebral concussion(50.8%), proliferous bone change(49.1%), chronic joint change(45.7%), periostitis(38.9%), nerve contusion(37.2%) and cerebral contusion(27.1%). In the case of Free-style wrestlers, they were in the following order: scratch(98.1%), muscle contusion(96.6%), muscle sprain(92.4%), sprain(91.5%), collateral ligament tension and fracture(88.6%), muscle stiffness(86.7%), joint sprain(84.9%), fissure(81.1%), fracture(79.2%), incised wound or cut, aural hematoma(71.6%), disc(69.8%), tendon fracture(56.6%), chronic joint change(49%), arthritis(47.1%), dislocation, proliferous bone change(45.2%), periostitis(41.5%), nerve contusion(37.7%), cerebral concussion(33.9%) and cerebral contusion(30.1%). (3) Times of Sports Injury According to 96.6% of Greco-Roman wrestlers and 98.1% of Free-style wrestlers, the most frequent time of injury occurrence was exercise time. While 38.9% of Greco-Roman wrestlers reported the experience of an injury on defense, 37.7% of Free-style wrestlers reported the experience of an injury both on offense and defense. While 100% of Greco-Roman wrestlers reported the experience of an injury on a cloudy day, 94.3% of Free-style wrestlers reported the experience of an injury on cloudy or raining days. The experience of an injury in the winter season was reported by 54.2% and 39.6% of Greco-Roman and Free-style wrestlers, respectively. The experience of an injury during afternoon exercise time was reported by 71.1% and 81.1% of Greco-Roman and Free-style wrestlers, respectively. (4) Causes of Sports Injury 'carelessness', 'an excessive desire to win' and 'the inability of coaches' were cited as the major causes of sports injury by 67.9%, 33.8% and 25.4 % of Greco-Roman wrestlers, respectively. Meanwhile, 'an excessive desire to win', 'lack of warm-up' and 'the inability of coaches' were cited as the major causes of sports injury by 64.1%, 37.7% and 30.1% of Free-style wrestlers, respectively. (5) Treatment of Sports Injury Both Greco-Roman and Free-style wrestlers reported that, when they had an injury, the emergency treatment was given by coaches and subsequent treatments were administered in hospital and that hot fomentation was applied as an emergency treatment. (6) Psychological state at time of sports injury Both Greco-Roman and Free-style wrestlers reported that, in case of an injury, they were thinking "I'm going to get better soon." Greco-Roman wrestlers' psychological reaction to injury was "What if I can't do as well as before? while that of Free-style wresters was "I'm afraid I will get hurt again." The most frequently recommended by both Greco-Roman and Free-style wrestlers as a solution to reduce injury was 'enough warm-up and finishing'. (7) To the question how many times they had an injury on the same location after treatment, 55.9% of Greco-Roman wrestlers and 47.1% of Free-style wrestlers answered "two to five times". Most of them reported they had experienced an injury in the same location after treatment. (8) Greco-Roman and Free-style wrestlers reported they participated in national tournaments 67 and 70 times respectively and they have ever given up an important match at least once due to injury.

신경섬유종증-제 2형의 진단과 치료 : 16례의 임상경험 Experience of 16 Cases

김정은,백선하,김종수,이상형,오창완,김동규,정희원,김현집,조병규,한대희,최길수 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.12

Neurofibromatosis type 2(NF-2) is a dominantly inherited disorder characterized by the occurrence of bilateral acoustic neurinomas and the frequent association of other central nervous system tumors. We present a retrospective review of 16 patients with NF-2 who were treated at our hospital from 1984 to 1995 in 13 cases, the diagnoses of Nf-2 were based on the criteria developed at the Consensus Development Conference of National Institute of Health in the United States, and in another 3 cases. the criterias of NF-2 were not fully satisfied, but the diagnoses of NF-2 were highly suspected. The average age of the patients was 27.6 years, ranging from 13 years to 56 years. The most common symptom was hearing difficulty : intervals between symptom onset and deafness ranged from 8 months to 6 years(mean : 2.9 years) One family of NF-2 was documented consisting of a sister a brother and their mother. Nine patients underwent operations on unilateral acoustic neurinomas : these were subtotally removed in eight patients and totally in one patient Among these patients five were deaf on the ipsilateral side at surgery. Among the other four patients with useful hearing before surgery, hearing was preserved to preoperative status in two patients. Four patients with diagnoses of meningioma, received operations to relieve mass effect with subtotal removal in two patients and total removal in the other two. Early diagnosis and treatment are the most important in the management planning of patients with NF-2 for reasons of early manifestation and rapid progression of the disease.

日中時間 變動에 따른 白鼠의 生理變化에 對한 補肝湯 瀉肝湯의 影響에 關한 硏究

金成圭,洪茂昌,金完熙 慶熙大學校韓醫科大學韓醫學硏究所 1986 慶熙韓醫大論文集 Vol.9 No.-