암세포 증식에 대한 YB-1 안티센스 올리고핵산염의 영향

김명성 ( Myung Sung Kim ),이완식 ( Wan Sik Lee ),박창환 ( Chang Hwan Park ),주영은 ( Young Eun Joo ),김현수 ( Hyun Soo Kim ),최성규 ( Sung Kyu Choi ),유종선 ( Jong Sun Rew ),정영도 ( Young Do Jung ),김세종 ( Sei Jong Kim ),안봉환 대한내과학회 2006 대한내과학회지 Vol.71 No.3

목적: YB-1은 PCNA, DNA 중합효소 및 MDR 유전자 등의 전사인자로 작용한다. YB-1 유전자는 정상 어른의 간에서는 발현되지 않지만 태아의 간이나 재생 중인 간에서는 발현이 현저히 증가되어서 세포의 증식과 밀접한 관련이 있음이 보고되었다. 본 연구에서는 YB-1 유전자의 안티센스 올리고핵산염을 이용하여 YB-1의 발현을 억제함으로써 암세포 증식을 차단 할 수 있는지를 실험하고 암치료를 위한 유전자 요법으로서의 가능성을 알아보았다. 방법: 세포주로는 Chang liver, HepG2, CT-26 세포를 사용하였고, 사람의 정상세포로는 섬유아세포와 내피세포가 혼재된 조직을 사용하였다. YB-1 유전자의 안티센스 올리고핵산염으로는 YB-1 cDNA의 변역 시작 부위에 상보적인 21mer 올리고핵산염을 제작하여 사용하였다. 세포의 성장은 MTT assay를 이용하였고, 유전자 발현은 Northern blot으로 분석하였으며, 세포주기 변화는 propidium iodide로 염색하여 유식세포분석기로 분석하였다. 동물실험에서는 CT-26 세포를 1.0×10(5)개씩 Balb/c 생쥐의 피하에 접종하여 종양을 유도하였다. 종양이 유도된 Balb/c 생쥐에 YB-1 안티센스 올리고핵산염을 꼬리정맥이나 종양조직에 주사한 후 종양의 크기를 측정하여 종양억제 효과를 관찰하였다. 결과: YB-1 안티센스 올리고핵산염은 CT-26 세포에서는 50 nM 이상 농도에서 Chang liver와 HepG2 세포에서는 10 nM 이상 농도에서 세포주의 성장을 강하게 억제하였지만 정상 조직세포의 성장에는 아무런 영향을 미치지 않았다. 안티센스 올리고핵산염을 DOTAP에 담지한 경우 세포성장 억제에 미치는 효과가 안티센스 올리고핵산염 단독 처치 보다 강하였다. 이때 YB-1의 발현은 증식이 억제된 세포주(Chang liver 및 CT-26)에서는 감소하였으나 정상조직세포에서는 변화가 없었다. 증식이 억제된 세포주에서 세포주기를 살펴보면 초기에 S phase가 감소함을 관찰 할 수 있었다. 꼬리정맥이나 종양조직에 YB-1 안티센스 올리고핵산염을 주입 시 종양의 크기가 유의하게 감소하였다. 결론: 이상의 실험 결과 YB-1 안티센스 올리고핵산염은 암세포의 성장을 저해하며 종양 동물모델에서 종양의 성장을 억제할 수 있음을 시사하였다. Background: Human YB-1 is a transcription factor that binds to the inverted CCAAT box in the promoter region of a variety of genes such as PCNA, DNA polymerase and MDR. In this study we evaluated the effect of YB-1 antisense oligonucleotides on tumor cell growth. Methods: Chang liver, HepG2 and CT-26 cells were cultured as immortalized cell lines. The MTT (3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyltetrazolium bromide) assay, Northern blot and flow cytometric analyses were used to determine cell growth, gene expression and cell cycle changes. In an animal model, CT-26 cells were injected into Balb/c mice to induce tumor; YB-1 antisense oligonucleotides were injected into the tail vein or tumor tissue of the mice; change of tumor size was then measured. Results: Phosphorothioated YB-1 antisense oligonucleotides suppressed the proliferation of the immortalized liver cells (Chang liver cells) and a variety of cancer cells (HepG2 and CT-26 cells); however, it did not inhibit normal cell growth. The DOTAP/antisense oligonucleotide mixture showed stronger effects on cell proliferation than did the antisense oligonucleotide alone. The YB-1 antisense oligonucleotide decreased specific expression of the YB-1 mRNA in the immortalized cancer cell lines. Flow cytometric analysis revealed that the inhibition of cell proliferation might have been due to a decrease in the S phase of the cell cycle. We found that in an animal tumor model, the administration of the YB-1 antisense oligonucleotide, in the vein or tumor tissues, decreased the tumor size significantly. Conclusions: These results suggest that the YB-1 antisense oligonucleotide may inhibit growth of a variety of cancer cells.(Korean J Med 71:293-301, 2006)

바닥충격음 감소를 위한 건조몰탈 개발과 현장 적용

박창환,조성현,고효진,김용로,김태정,Park, Chang-Hwan,Cho, Sung-Hyeon,Ko, Hyo-Jin,Kim, Yong-Ro,Kim, Tae-Jeong 한국건설순환자원학회 2022 한국건설순환자원학회지 Vol.17 No.3

Myristyl 지방족기를 함유하는 제4급 암모늄염에 의한 PET 섬유의 감량가공 효과

박창환,근장현,김영근,박홍수 ( Chang Hwan Park,Jang Hyoun Keun,Young Geun Kim,Hong Soo Park ) 한국유화학회 1994 한국응용과학기술학회지 Vol.11 No.2

MDABM and MDAEW, as a accelerating weight loss agents, were prepared by adding water to myristyldimethylbenzylammonium bromide(MDAB) and myristyldmethylethylammonium ethyl sulfate(MDAE) synthesized. As a result of weight loss finishing of the MDABW and MDAEW with NaOH on PET fiber, the ratio of weight loss of MDABW was very larger than that of MDAEW. This result showed that quaternary ammonium bromide had higher weight loss effect than quaternary ammonium sulfate, and the ratio of weight loss was greatly varied with the kind of quaternary ammonium salts used. In these conditions, proper treatment concentration, treatment time, and treatment bath ration were about 8g/l, 60~90min, and 40:1~50:1, respectively.

Permanent Press 가공용 실리콘 유연제의 합성과 그의 특성화

박창환,김승진,김영근,박홍수 ( Chang Hwan Park,Seung Jin Kim,Young Geun Kim,Hong Soo Park ) 한국유화학회 1995 한국응용과학기술학회지 Vol.12 No.1

Silicone softner(SSN-3) for permanent press(PP) finish was prepared by blending beef tallow hardened oil for improving softness, water, the emulsion, which was synthesized from pentaerythritol monostearate as a softening component and silicone oil KF-96 as a lubricating component. The prepared SSN-3 and the PP finishing resin were applied to PP finishing cotton broad cloth and P/C gingham samples using one bath method. The properties such as crease recovery, tear strength, bending resistance test were tested. The samples treated with SSN-3 and PP finishing resin have improved properties, compared with nontreated samples, those treated only with PP finishing resin, those treated with commercial PP finishing softners and PP finishing resin. Also from the bending resistance test, the two kinds of fabric samples treated with SSN-3 of 3% showed grade 5 and these were good enough in feeling.

대학교육의 도의

박창환,Park, Chang-Hwan 한국대학교육협의회 1984 大學敎育 Vol.12 No.-

악관절내장증의 진단에 있어 Dynamic MRI의 효용

박창환,김명래,김선종,정은철,Park, Chang-Hwan,Kim, Myung-Rae,Kim, Sun-Jong,Cheong, Eun-Chul 대한악안면성형재건외과학회 1994 Maxillofacial Plastic Reconstructive Surgery Vol.16 No.3

The Magnetic Resonance Imaging has been used widely to evaluate the disk position without any interruption of the TMJ structures, and the Dynamic MRI presenting computed serial imaging or the video-recorded simulation images is thought to be very effective to evaluate the disk position under function. This is to study the correlation between the clinical diagnosis and the findings of Dynamic MRI for the diagnosis of internal derangement of the temporomandibular joints. 30 joints(15 patients) were examined clinically, and the movement of TMJ meniscus was reviewed in the dynamic MRI. The comparative results are as follows : 1. All internal derangements of TMJ disk displacement without reduction were consistent with MRI findings. 2. 5 joints (50%) of disk displacements with reduction could not be confirmed by MRI findings. 3. The disk displacements in MRI were found in 55% of painful joints, 50% of clicking joints, and 70% of the joints with restricted movement. 4. The reliability of MRI for the diagnosis of TMD was evaluated as 77% ; 24 of 30 joints who presented with clinical diagnosis of TMD. 5. MRI is very reliable to diagnose the disk displacement without reduction, but it is rather not so effective to diagnose the early derangement or muscle disorders.

CA 19-9 상승을 동반한 무증상의 위 이소성 췌장

김형일 ( Hyung Il Kim ),박창환 ( Chang Hwan Park ),전충환 ( Chung Hwan Jun ),이기훈 ( Gi Hoon Lee ),김현수 ( Hyun Soo Kim ),최성규 ( Sung Kyu Choi ),유종선 ( Jong Sun Rew ) 대한내과학회 2008 대한내과학회지 Vol.74 No.6

저자들은 CA 19-9 상승을 동반한 무증상의 위 이소성 췌장을 내시경 점막하 박리술을 통하여 확진 및 제거하였으며 추적 검사에서 CA 19-9의 정상화를 경험하였기에 보고하는 바이다. Ectopic pancreas is an uncommon clinical finding, and it is rare for heterotopic pancreatic tissue to cause symptoms. The most common sites for ectopic pancreas are the submucosal layer of the stomach and the small intestine. An ectopic pancreas is subject to various pathological changes occurring in the pancreas itself, namely cyst, pancreatitis, hemorrhage, abscess, necrosis, and neoplastic changes. We report a case of a gastric ectopic pancreas associated with elevated serum carbohydrate antigen 19-9 (CA 19-9). We performed endoscopic submucosal dissection for histologic diagnosis and removal of the submucosal tumor in the stomach, and histological examination confirmed ectopic pancreas tissue. One months later, serum CA 19-9 declined to a normal level.(Korean J Med 74:663-666, 2008)

진공청소기용 팬-모터의 시스템-레벨 분석

박창환(Chang-Hwan Park),이종대(Jong-Dae Lee),신동진(Dong-Jin Sheen) 한국유체기계학회 2018 유체기계 연구개발 발표회 논문집 Vol.2018 No.11

구사론주(kosakara) 세친(Vasubandhu)과 『능가경』(Lankavatarasutra) -『석궤론(釋軌論)』(Vyakhyayukti)의 『릉가경(楞伽經)』 인용을 중심으로 본 『능가경』과 세친 사상의 영향관계-

박창환 ( Chang Hwan Park ) 인도철학회 2011 印度哲學 Vol.0 No.32

슈미트하우젠(Schmithausen 1992)은 『능가경』의 편찬자가 세친의 『유식삼십론』을 인용한다고 말함으로써 경과 논의 관계에 대한 기존의 통념을 뛰어넘는 획기적인 주장을 한다. 그런데 세친의 『석궤론』에는 『능가경』으로 추정되는 익명의 대승경이 인용되는데, 이것이 만일 『능가경』임이 판명된다면 슈미트하우젠이 주장하는 것과는 반대로 오히려 세친이 『능가경』을 학습하였으며 대승 전환 이후의 그의 유식사상의 전개 과정에 『능가경』의 영향력이 있었음을 추정할 수 있게 된다. 따라서 본고는 세친과 『능가경』의 연대적 선후문제를 확정할 수 있는 문헌적 전거로 세친 『석궤론』의 『능가경』 관련 구절을 지목하고 그 인용 원칙과 내용을 기존의 『능가경』판본들과 비교·분석한다. 그리고 세친이 「게송품」(Sagathakam)을 포함한 위역(魏譯) 이래의 판본과 유사한 『능가경』을 활용했을 것이라는 결론에 도달한다. The relation of Vasubandhu and the Lankavatarasutra (LAS) has long been a controversial issue among scholars, which has resulted in nothing definite so far. It is well-known that the LAS contains two particularly close parallels to Vasubandhu`s Trimsika (Tr). Based upon these parallels, Lambert Schmithausen claimed in his article entitled "A Note on Vasubandhu and the Lankavatarasutra (1992) that "LAS draws, after all, upon Tr, and merely pretends to quote from a Sutra because as a Sutra LAS cannot of course quote a Sastra." This entails that the LAS postdates Vasubandhu, which confirms the hypothesis of such Japanese scholars as Ui Hakuju. The present paper aims to provide counter-examples to this position, arguing that it is probably Vasubandhu who quotes from LAS and utilizes its cittamatra ideas in his post- Abhidharmakosa Yogacara treatises. In order to prove this, what I do first is to critically examine Schmithausen`s arguments with reference to the two parallels between LAS and Tr, which leads to the conclusion that Schmitausen`s arguments are not definitive to prove the dependency of LAS upon Tr. Then I go on to examine a more substantial piece of evidence culled from the Vyakhyayukti where Vasubandhu quotes two sets of verses (gatha) from an anonymous Mahayanasutra as an explicit meaning (nitartha) of Mahayana Buddhism, a sutra which in fact has been identified as LAS by scholars such as Yamaguchi Susumu. Vasubandhu treats this anonymous sutra as a sutra of an explicit meaning (nitarthasutra) in parallel to the Samdhinirmocanasutra. So this paper tries to substantiate whether this sutra is really LAS by comparing it with currently available Chinese translations and Sanskrit recension. This analysis leads to the conclusion that in the Vyakhyayukti Vasubandhu quotes from a Sanskrit version of LAS close to the currently available Wei-translation, which contains both the main body of the sutra that consists of prose and verse parts and the verse portion (Sagathakam) that solely consists of verses (gatha). If this is the case, we have to examine all over again the doctrinal influence of LAS over Vasubandhu`s thoughts on Vijnaptimatra, which seems to have been inspired by the notion of cittamatra in LAS.

Cooperative SFBCOFDM 시스템을 위한 파일럿 톤 기반의 채널 추정 기법

박창환(Chang Hwan Park),고요한(Yo Han Ko),이희수(Heesoo Lee),조용수(Yong Soo Cho) 한국통신학회 2008 韓國通信學會論文誌 Vol.33 No.9C

본 논문에서는 STO(Symbol Timing Offset)를 갖는 cooperative SFBC-OFDM 시스템에 적합한 파일럿 톤 기반의 채널 추정 기법을 제안한다. Cooperative SFBC-OFDM 시스템에서는 MS(Mobile Station)의 위치에 따라 RS(Relay Station)와 MS간 STO가 다르게 발생하며 이 STO가 위상 회전 형태로 왜곡을 발생시키기 때문에 orthogonal 파일럿 사용시 위상과 크기에 대하여 선형 보간을 취하여 채널을 추정하는 방법을 제안한다. 또한 orthogonal code를 사용하는 파일럿 구조에서는 비선형 방정식의 해를 구함으로써 채널 추정을 수행하는 방법을 제안한다. STO를 갖는 cooperative SFBC-OFDM 시스템에서 제안된 기법을 적용할 경우에 채널 추정 성능이 크게 개선될 수 있음을 모의실험을 통하여 확인한다. In this paper, a channel estimation technique based on pilot tones is proposed for cooperative SFBC-OFDM systems with a STO(Symbol Timing Offset). In a cooperative SFBC-OFDM system, the STO between RS(Relay Station) and MS(Mobile Station) varies depending on the location of MS. Since the STO causes distortion in the form of phase rotation, a channel estimation technique based on linear interpolation with respect to phase and amplitude is proposed for the case of orthogonal pilot allocation. Also, a channel estimation technique is proposed by solving nonlinear equation for the case of pilot structure with orthogonal code. It is shown by computer simulation that the performance of channel estimation can be significantly improved when the proposed techniques are applied to cooperative SFBC-OFDM systems with STO.