Activation of Transient Receptor Potential Melastatin Family Member 8 (TRPM8) Receptors Induces Proinflammatory Cytokine Expressions in Bronchial Epithelial Cells

김주희,Young Sook Jang,Hwan Il Kim,박지영,박성훈,Yong Il Hwang,Seung Hun Jang,Ki-Suck Jung,Hae Sim Park,Choon-Sik Park 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.4

Purpose: Cold air is a major environmental factor that exacerbates asthma. Transient receptor potential melastatin family member 8 (TRPM8) is a cold-sensing channel expressed in the airway epithelium. However, its role in airway inflammation remains unknown. We investigated the role of TRPM8 in innate immune responses in bronchial epithelial cells and asthmatic subjects. Methods: The TRPM8 mRNA and protein expression on BEAS2B human bronchial epithelial cells was examined by real-time polymerase chain reaction (PCR), immunofluorescence staining and western blotting. Additionally, interleukin (IL)-4, IL-6, IL-8, IL-13, IL-25 and thymic stromal lymphopoietin (TSLP) levels before and after menthol, dexamethasone and N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl) piperazine-1-carboxamide (BCTC) treatments were measured via real-time PCR. TRPM8 protein levels in the supernatants of induced sputum from asthmatic subjects and normal control subjects were measured using enzyme-linked immunosorbent assay, and mRNA levels in sputum cell lysates were measured using real-time PCR. Results: Treatment with up to 2 mM menthol dose-dependently increased TRPM8 mRNA and protein in BEAS2B cells compared to untreated cells (P < 0.001) and concomitantly increased IL-25 and TSLP mRNA (P < 0.05), but not IL-33 mRNA. BCTC (10 μM) significantly abolished menthol-induced up-regulation of TRPM8 mRNA and protein and IL-25 and TSLP mRNA (P < 0.01). TRPM8 protein levels were higher in the supernatants of induced sputum from asthmatic subjects (n = 107) than in those from healthy controls (n = 19) (P < 0.001), and IL-25, TSLP and IL-33 mRNA levels were concomitantly increased (P < 0.001). Additionally, TRPM8 mRNA levels correlated strongly with those of IL-25 and TSLP (P < 0.001), and TRPM8 protein levels were significantly higher in bronchodilator-responsive asthmatic subjects than in nonresponders. Conclusions: TRPM8 may be involved in the airway epithelial cell innate immune response and a molecular target for the treatment of asthma.

Scoparone from Artemisia capillaris Inhibits the Release of Inflammatory Mediators in RAW 264.7 Cells upon Stimulation Cells by Interferon-${\gamma}$ Plus LPS

Jang Seon Il,Kim Young-Jun,Lee Woo-Yiel,Kwak Kyung Chell,Baek Seung Hwa,Kwak Gyu Beum,Yun Young-Gab,Kwon Tae-Oh,Chung Hun Taeg,Chai Kyu-Yun The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.2

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin $E_2\;(PGE_2)$ upon stimulation by IFN-${\gamma}$/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-${\gamma}$/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-${\alpha}$, interleukin (IL)-$1{\beta}$ and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and $PGE_2$ in macrophages by inhibiting iNOS and COX-2 expression.

Scoparone from Artemisia capillaris Inhibits the Release of Inflammatory Mediators in RAW 264.7 Cells upon Stimulation Cells by Interferon-γ Plus LPS

Seon Il Jang,Young-Jun Kim,Woo-Yiel Lee,Kyung Chell Kwak,Seung Hwa Baek,Gyu Beum Kwak,Young-Gab Yun,Tae-Oh Kwon,Hun Taeg Chung,Kyu-Yun Chai 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.2

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) upon stimulation by IFN-γ/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-γ/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression.

가감보중익기탕의 LPS 유도 염증성 매개물에 대한 억제 효과

장선일,김형진,김용준,배현옥,정헌택,정옥삼,김윤철,윤용갑 대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.3

Kagam-bojungikgitang is the water extracts prepared from Ginseng Radix, Astragali Radix, Angelicae gigantis Radix, Astractylodis Rhizoma alba, Aurantii nobilis Pericarpium, Glycyrrhizae Radix, Artemisiae iwayomogii Herba, and Scutellariae Radix. This is a modified prescription of Bojungikgitang, which has been used for the treatment of indigestion, and immunological disease in oriental countries. In this study, the effects of Kagam-bojungikgitang and Bojungikgitang on the production of prostaglandin E_2 (PGE_2) and the expression of cyclooxygenase-2 (COX-2) were examined using Raw 264.7 macrophages activated with lipopolysaccharide (LPS). Both prescriptions dose-dependently reduced the release of PGE_2 and expression of COX-2 caused by stimulation of LPS without cytotoxic effect. Kagam-bojungikgitang's inhibitory effects were better than Bojungikgitang in PGE_2 production and COX-2 expression. Moreover, Kagam-bojungikgitang also attenuated markedly the production of tumor necrosis factor (TNF)-α, and IL-6 than Bojungikgitang in LPS-stimulated RAW 264.7 macrophages. These results suggest that Kagam-bojungikgitang decrease PGE_2 and pro-inflammatory cytokine production in macrophages and these properties may contribute to the anti-inflammatory activity of Kagam-bojungikgitang.

폐혈관상이 유지된 선천성 낭성 선종양 기형

배일헌,한기석,차상훈,김성진,박길선,이건국,홍장수 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

선천성 낭성 선종양 기형은 태생 16주 이후 말단세기관지의 형성이 중단되어 미성숙기관지들과 중배엽성분들이 과도하게 증식하여 발생하는 드문 선천성 폐질환이다. CT소견은 낭종의 크기 숫자, 내용물 등에 따라 다양하나, 병변내에 정상적인 폐혈관이 보인 예는 전세계적으로 보고된 적이 없었다. 저자들은 역동적 흉부 CT상 균일한 저음영의 병변내에 정상 폐혈관상이 유지된 선천성 낭성 선종양 기형 1예를 경험하였기에 보고한다. Congenital cystic adenomatoid malformation(CCAM) is a rare pulmonary disease that is thought to be developed by abnormal proliferation of immature alveoli and other mesenchymal component from the abrupt halting of the terminal alveoli formation after 16 weeks of gestation. On CT scan, there are no consistencies regarding to size, numbers, and its contents but no cases were reported in past showing normal pulmonary vascularities. We reports a case of CCAM that showed normal pulmonary vascularities within the hypodense lesions on a dynamic chest CT

예도(銳刀)의 미시사적 관점으로 바라본 무예실제에 관한 분석

송일훈,이태현,장성호,장경태,김대양 龍仁大學校 武道硏究所 2011 武道硏究所誌 Vol.22 No.1

예도(銳刀)에 관한 보다 정확한 고찰은 오늘날 전통무예단체들이 『武藝圖譜通志』를 통한 전통무예의 실제를 복원 및 재현함에 있어 크나큰 도움이 될 것이다. 이는 예도(銳刀)가 전통무예사에 대한 단초적 역할을 제공할 수 있는 초석이 될 수 있기 때문이다. 따라서 이 연구에서는 예도의 설(說: 해설(解說))과 기(技: 기예(技藝))의 형식적 분석과 전통무예의 복원에 관한 이론적 배경을 정립하기 위하여 고서와 도록 등의 다양한 자료를 검토‧분석하였는데 이는 다음과 같은 결론을 도출하였다. 첫째, 예도(銳刀)의 종합적 분석을 통한 예도의 형식은 병기도식(兵器圖式)․설(設) 11개․예도보(銳刀譜)․예도총보(銳刀總譜)와 예도총도(銳刀總圖)로 구성되어 있음을 알았다. 둘째, 예도는 본국검과 더불어 조선을 대표하는 한국 고유의 전통검법으로 임진왜란을 계기로 새롭게 발굴 정립되었으며 정조가 재위했던 1800년대까지 약 200년 걸쳐 전승되면서 민족의 재산과 생명을 지켜왔던 국방무예(24반 무예)였다. 셋째, 국난을 극복하고 국토를 수호하려는 선조들의 의지로 완성된 24반 무예 속에 녹아 든 활달한 몸짓 속에는 민족자존의 의지와 불굴에 투혼이 배어 있다. 이와 같이 오늘날 한․중․일의 칼 쓰는 법을 비교해 볼 때, 『武藝圖譜通志』의 예도가 갖는 의미와 가치는 대단히 귀중하다 할 수 있겠다. 이상과 같이 종합해 볼 때, 앞으로 이 한국무예 예도(銳刀)에 관한 심도 있는 연구가 이루어질 때 비로소 예도(銳刀)가 세계적인 보편무예로 발전하는 중요한 기반이 될 것으로 기대하는 바이다.

기흉 후 발생한 뇌동맥 공기 색전증 : 1예 보고

배일헌,한기석,차상훈,김성진,박길선,홍장수 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

뇌혈관내 공기 색전증은 뇌혈관 조영술, 혈관내 도자술 시행시 발생하여 매우 심각한 합병증을 유발할 수 있다. 그러나 기흉의 합병증으로 발생한 경우는 보고된 적이 없다. 저자들은 기흉에 의하여 발생한 뇌혈관내 공기 색전증 1예를 경험하였기에 보고한다. Cerebral arterial air embolism is a potentially fatal event that may occur during the cerebral angiography and intravascular catheterization. The CT findings of cerebral arterial air embolism following pneumothorax have not been reported. We report a case of cerebral arterial air embolism following pneumothorax, and describe the CT findings.

2-Deoxy-D-Glucose에 의한 대사성 쇼크가 무우스의 면역 반응에 미치는 영향

김양숙,장선일,박래길,조성경,정헌택 최신의학사 1991 最新醫學 Vol.34 No.2

성인 급성 백혈병의 치료에 관한 연구

김종완,장준,신승훈,윤일국,박철신,김삼용 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

19 adult ALL patients were treated with follwing regimens; VP regimen to 5 patients, VPA regimen to 4 patients, multi-drug combined intensive therapy of Hoelzer regimen to 10 patients. The results of therapy are as follows: 1. Complete remission occured in 3 patients on VP regimen(60%), 4 patients on VPA regimen (100%), 8 patients on Hoelzer regimen (80%), respectively, and overall complete remission rate was 78.9%. 2. Mean survival time of total patients was 13.8 months, and in complete remission group, it was 16.2 month, but in non-complete remission group, it was 1.8 month. 3. Factors favorable for complete remission were absence of organomegaly(P<0.05) and initial leukocyte count under 30,000/㎟(P<0.01), factors favorable for the survival duration were initial leukocyte count under 30,000/㎟(P<0.05) and remission within 4 weeks of treatment.(P<0.01). 4. Recurrence rate were 40% in VP regimen, 50% in VPA regimen, and 20% in Hoelzer regimen. 5. During the remission induction period, common side effects of chemotherapy were alopecia, nausea, vomiting, mucositis, and general weakness and it was severe in VPA regimen and Hoelzer reimen than in VP regimen. But no significant difference was noted between VPA regimen and Hoelzer regimen. From the above results, we could confirm the importance of multiple drug combined intensive chemotherapy of remission regimen and periodic reinduction chemotherapy.

신생아 말초혈액 T 림프구의 아형 분석

최두영,김종덕,장선일,이신은,조성경,정헌택 최신의학사 1990 最新醫學 Vol.33 No.12