Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

유리纖維 補强 콘크리트 流動 特性에 관한 硏究

朴承範,任昌悳,尹義植 忠南大學校 産業技術硏究所 1989 산업기술연구논문집 Vol.4 No.1

Concrete is an excellent structural material, but it has many problems due to brittle behavior, weak resistance in tension, flexibility and toughness. In order to improve these problems, usually fiber reinforced concrete is used. But in the case of GFRC insufficient workability is caused by using glass fiber and there is lack of studying on it. This report of the following concrete of GFRC was experimented by the different W/C, S/A, glass fiber contents and maximum size of Aggregates. The results of this study is as follows; 1) Optimum S/A ratio is 0.6 at the glass fiber contents of from 0.25% to 0.75% in GFRC, but it cannot be obtained optimum S/A ratio at over 0.75. 2) Even though increasing the W/C ratio, we can get more slump, but slump is much more influenced by fiber contents in GFRC rather than increasing the W/C ratio. 3) Workability of GFRC is much more influenced by fiber contents than fiber length. 4) Workability of GFRC is a little improved by the bigger maximum size of aggregates.

지역문화정보시스템의 개발방안에 관한 이론적 고찰

박의준,윤평현 전남대학교 호남문화연구소 2002 호남학 Vol.30 No.-

The purpose of this study is to examine the effective way to designing the "Regional Culture Information System". The designing the regional culture information system can be defined as "assembling the information and database in the region and analyzing the correlation between regional characteristics and regional culture, each culture's attribute" For the designing the effective regional culture information system, we need follow the next approaching step. First, collect regional information contents (including physical, social, economical properties) and designing the regional information system. Second, carry out the systematic and depth field survey about the detail field forming the regional culture. Third, build the regional culture atlas which visualize the regional culture information. Fourth, design the regional culture information system by combine the regional information system and regional culture atlas, regional culture database. This regional culture information system is 3-dimensional tool which can analyze the correlation between regional characteristics and regional culture, each culture's attribute, spatial temporal variations of regional culture. The regional culture information system helps not only the academic study, but also establish the culture strategies and place marketing of the region.

Fordyce Granules

박의수,송준영,최윤애 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

Fordyce granules are small yellow spots that are multifocal and slightly elevated. They may occur in clusters or coalesce to from yellow plaques. They are bilaterally symmetrical and are located most frequently on the buccal mucosa of the cheeks, the inner surface of the lips, and on the mucosa distal to the mandibular molars. Fordyce spots are extremely common: probably 80% of the population have them. They are often not noticeable in children until after puberty, and they seem to be more obvious in males, in patients with greasy skin and in the elderly. The granules are totally benign, though the occasional patient or physician becomes concerned about them or misdiagnoses them as thrush, lichen planus or other diseases. We present two cases of Fordyce granules a 64 and a 50 year-old male who have cancer phobia, showing multiple involvement of minute yellowish white colored spots on the buccal mucosa and inner surface of the lip. Histopathologic finding shows a group of small but mature sebaceous lobules situated around a small sebaceous duct leading to the surface epithelium. We did not give any specific treatment but gave reassurance, presently under observation.

高等學校 生物의 遺傳工學 槪念 導入을 위한 實驗 Model

박상익,윤의수,김종균 공주대학교 사범대학 과학교육연구소 1995 과학교육연구 Vol.26 No.1

제 4차 교육과정 이후 제 5차 교육과정으로 개편되면서 생물 교과서에 유전 공학 개념 및 응용면이 더욱 강조 되었고, 제 6차 교육과정에서는 더욱 심화된 내용이 소개될 것으로 본다. 특히 일본의 고교 생물에서는 유저너 공학에 관한 내용을 학생들이 실험 할 수 있게 수록되어 있다. 그리고 앞으로의 자연과학은 유전공학 분야에서도 눈부신 발전을 가져올 것으로 기대되면 이에 맞추어 고등학교에서 유전공학 개념의 이해를 위한 유전자의 삽입, 형질 전환, 조직 배양 등의 실험을 실시하는 것은 학생들에게 많은 흥미와 관심을 갖게 하며 탐구력 신장에도 도움을 줄 것으로 생각한다. 본 연구는 제 5차 교육과정에 따른 고등학교 생물 교과서에 소개된 유전공학 내용을 분석한 결과 및 잎 절편체가 모상근으로 형질전환되는 기초 실험의 결과를 토대로 다음과 같은 결론을 내리고 탐구적 생물교육 자료로 삼고자 한다. 1.Agrobacterium tumefacieus, Agrobacterium rhizogenes을 이용한 모상근 발생에 관한 기초 실험의 특징은 형질전환체를 가시적인 뿌리로 얻을 수 있어 관찰이 용이하다. 2.형질 전환이 되었음을 GUS 활성 검사에 의해 쉽게 증명할 수 있어 유전공학 개념 도입을 위한 실험으로 고등학교에 도입하는 데 어려움이 없을 것으로 본다. 3.실험에 이용한 균은 감염 부위에 모상근을 형성하는 이외는 다른 병증을 나타내지 않기 때문에 대외적인 문제 없이 학교의 실험실에서도 취급할 수 있다. 4.유전 공학 실험에 필요로하는 화합물은 일선 학교에 대부분이 준비되어 있으며 kanamycin, Claforan은 미량 필요한데 쉽게 적은 비용으로 구입할 수 있다. 5.실험 기구는 간이 배양실만 고안 사용하면, 고등학교에 준비되어 있는 기구를 이용하여 해결이 가능하다. 유전 공학에 관한 실험을 하는데 실험실의 환경 조건이나 다인수 학급에서 어려움이 있더라도, 학생들에게 실험을 통해 유전 공학 개념을 형성시키는 기회를 갖게하는 것은 매우 중요하다. 이렇게 하여 자연과학에 대한 흥미와 관심을 높여가는 것은 우리나라의 기초과학 발전에 도움이 될 것으로 본다. The concept of biotechnology and its practical application is much more emphasized in the fifth curriculum than in the fourths and further intensified contents are supposed to be introduced in the sixth. Especially highschool biology in Japan, the contents on the biotechnology are recorded for the students to experiment and the future natural science is expected to develop brilliantly in a field of biotechnology. Therefore, students' experiments on gene insertion, transformation, tissue culture and so on, for their understanding of biotechnology concepts, are thought to stimulate their curiosity and extend their spirit of inquiry. This study is meant as an teaching aid, and reaches the following conclusions on the basis of the results of the biotechnology contents introduced in the fifth highschool biology texts and those of basic experiments on the transformation from explant to hairy root. The characteristic of basic experiments on hairy root growth used by Agrobacteriurm tumefacieus, Agrobacterium rhizogenes is that the observation is easy, since the transformational object is a visible root. The experiments are likely to be introduced without difficulty for highschool biotechnology concepts, since the transformation is easily proved by the GUS activation test. The germs used in experiments don't cause other diseases except forming hairy roots in infected parts, so they can be treated in school laboratories without outside interferance. Almost all schools are equipped with the complex materials needed for biotechnology experiments and kanamycin and claforan, just a little quantity of which is needed, can be purchased at a low price. The instruments can be replaced with the preequipped ones in school, only the temporary growthchamber needs to be devised. Though there are difficulties in biotechnology experiments because of unsufficient condition in laboratories or classes made up of a large number of students, it is very important for the students to have opportunities to form biotechnology concepts through experiments. To promote curiosity and interest in natural science in this way can be helpful in national basic science development.

忠州市 地域特性을 反暎한 共同住宅 計劃硏究 : 충주지역 거주자의 APT 선호특성을 중심으로

朴碩鉉,朴義權,柳顯紀,尹勝照,孫泰鎭,金基洙 충주대 2003 産業科學論文集 Vol.11 No.-

The purpose of this research is to analyze the correlation of the characteristics of dwellers and residential preference in apartment housing through the case study of 340 samples in 25 housing estates which were built in Chung-ju city The contents of this research consist of two main parts. The first part is to identify characteristics of dwellers, which might have an influence on residential preference in apartment housing. The second part is to identify the main physical elements of residential preference that can be influenced by the personal characteristics. Thus, this research will contribute to the establishment of the planning methods which increase residential preference in apartment housing, while promoting the quality of residential environment in Chung-ju city.

유도 '되치기 본'의 개선 방안에 관한 연구

김의환,김도준,김규수,김관현,김종달,최종삼,조용철,박순진,윤익선,안병근,정 훈,김미정,한성철 龍仁大學校 武道硏究所 1999 武道硏究所誌 Vol.10 No.1

The purpose of this study was to reform practically a Korean Judo's Doechigi-Bon(Forms of Counterattack, judo's Gaeshi no Kata, Judo's Uradori no Kata, Judo's Gonosen no Kata) that was established in 1955 Korea, according to changing of techniques by Judo's modernization, in order to have Judo's carefulness and systematic diffusion. Reform procedure of Judo's Doechigi-Bon was 1st stage, Questionnaire survey 303 judokas, 2nd stage, Technical seminar by judo experts(12 judo professor) 4 times, 3rd stage, wording report for reform, 4rd stage, Discussion and judgement of Teaching and Judgement commission of Korean judo Association(KJA), 5th stage, Public hearing for reform in KJA, 6th stage, Report and decision of board of directors in KJA, 7th stage, public publication of Judo News(No.53) in KJA. Basic principle of reform of Judo's Doechigi-Bon were as table 1. Table 1. Basic principle of reform of Judo's Doechigi-Bon ------------------------------------------------------------------------------- Items Reformed Key Points of Judo's Doechigi-Bon ------------------------------------------------------------------------------- 1. Structure 1) Grand classification : Classified by 3 parts(1,2,3Gyo) 2)Medium classification : Te waza, Goshi waza, Ashi waza devided per each part(Gyo) 3)Sub-classification : Classified five techniques per each part(Gyo) 2. Contents 1) Selected established techniques as possible 2) Considered rationalty and overlapping of counterattack techniques 3. Decision of Conterattack techniques 1) Refered to results of Basic Questionnaire survey 2) Priority to decisions of Judo expert technical seminar -------------------------------------------------------------------------------- Reformed Korean Judo's Doechigi-Bon(Forms of Counterattack techniques-KJA, 1999) are as follows: 1. Gyo; ① Uki otoshi -> Uchi mata ② Harai goshi -> Harai goshi gaeshi ③ O soto gari -> O soto gake ④ Ko uchi gari -> Sasae tsurikomi ashi ⑤ O uchi gari -> Ko soto gari 2. Gyo; ① Ippon seoi nage -> Okuri eri jime ② Tsuri domi goshi -> Uki waza ③ Okuri ashi harai -> Okuri ashi harai ④ Ko soto gari -> Tai otoshi ⑤ Hiza guruma -> Hiza guruma 3. Gyo; ① Kata guruma -> Sumi gaeshi ② Tai otoshi -> Ko soto gari ③ Hane goshi -> Harai tsurikomi ashi ④ Uchi mata -> Tai otoshi ⑤ Tomoe nage -> O uchi gari

일 대학 병원 마취 · 회복실 간호사의 간호활동 분석

강윤경,김경미,김연이,박혜옥,서광희,송숙녀,이현숙,조의영 대한간호행정학회 2006 간호행정학회지 Vol.12 No.1

Purpose: This study was aimed to specify roles of nurses from the anesthesia and recovery room by analyzing nursing activities as well as anesthesia nursing during surgery. Method: The objects were 12 RNs working in the recovery room of a university hospital located in Incheon. Self-report was performed by measuring stop-watch for five days from May 17th to May 21st in 2004. Research method was designed to record the time and frequency of the nursing activity from anesthesia and recovery room based on nurses' statement and other references for five days. The data were analyzed with descriptive statistics, ANOVA, and t-test via SPSS Win 10.0 program. Result: Nursing activities in anesthesia recovery room were classified into two different characteristics such as direct and indirect nursing activity. The activities consisted of 11 direct nursing areas and 39 nursing activities in anesthesia preparation room. The indirect nursing was classified into 8 nursing areas and 32 nursing activities. The direct nursing was classified into 12 nursing areas and 55 nursing activity. Also, the indirect nursing was classified into 7 nursing areas and 21 nursing activities in recovery room. In terms of prevalence of nursing activities in the anesthesia preparation room, observation and cooperation of anesthesia was the most prevalent activity, drug and eqipment management the second prevalent, and drug administration the third. On the other hand, in the recovery room, the most prevalent activity was vital sign checking and observation, the second most prevalent activity informative activity, and the third body temperature control. Nursing activity time was recorded according to the nursing characteristics. In the anesthesia preparation room, the direct nursing spent for 8092.20 minutes was larger than the indirect nursing spent for 7198.50 minutes. Also, in the recovery room, the direct nursing spent for 2361.16 minutes was larger than the indirect nursing spent for 1134.13 minutes. 4. Nursing activity time was compared to duty shifts. In the anesthesia preparation room, the direct nursing was more prevalent on day shift and the indirect nursing was prevalent on evening shift. However, in the recovery room, both direct and indirect nursing activities were prevalent on day shift. Conclusion: The role of anesthesia and recovery room nurses was analysed according to the time, frequency, and its characteristics.

원자로압력용기장 용접열영향부의 미세조직 변화가 Magnetic Barkhausen Noise 거동에 미치는 영향

김주학,윤의박,문종걸,박덕근,홍준화 韓國非破壞檢査學會 1998 한국비파괴검사학회지 Vol.18 No.4

최근 자기적 특성의 변화가 재료의 미세조직적 상태에 민감하다는 보고가 있다. 재료의 미세조직은 상(phase), 결정립의 크기, 석출물 등의 다양한 인자로 구성되며, 본 연구에서는 이러한 인자들을 이용하여 재료의 특성과 Barkhausen noise(BN)의 관계를 연관시키기 위한 시도를 하였다. ASTM A 508 Gr.3 원자로압력용기강을 대상으로 용접열영향부의 미세조직적인 변화를 일곱가지 대표적인 영역으로 구분하였고, 각 영역에서의 미세조직과 기계적특성의 변화를 파악한 후, 각 조건별로 BN을 측정하였다. 통상의 보자력이나 잔류자화 같은 자기적 성질은 큰 변화가 없었지만, BN은 미세조직의 상태에 따라 현격한 변화를 보였다. 결정립과 석출물의 크기가 증가함에 따라 BN은 증가하였고, tempered martensite 보다는 tempered bainite 조직에서 높은 BN을 나타냈다. Recent study has demonstrated that some magnetic properties are sensitive to the microstructural state of material. The ASTM A 508 Gr. 3 reactor pressure vessel steel has various microstructural changes including martensitic and bainitic phases, and various sizes of grain and precipitates in the weld heat-affected zone (HAZ). To correlate the microstructural state with Barkhausen noise (BN), specimens were prepared through simulating various weld thermal cycles using a thermal simulator. The conventional magnetic properties, i.e. coercive force, remanence and maximum induction, did not change significantly. whereas the BN amplitude and energy during a magnetization cycle changed markedly with microstructural state. The BN increased with increasing grain and carbide sizes, and the tempered bainite structure showed higher BN parameter than tempered martensite.

소화성 궤양에서 삼제 병합 요법의 저용량에 의한 Helicobacter pylori 제균률과 궤양 치유효과

김예리,박봉안,이재동,이종혁,김인숙,박형석,진춘조,김상윤,박의우 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Helicobacter pylori is the major cause of histologic chronic gastritis and is strongly associated with peptic ulcer disease, gastric cancer and lymphoma. Several recommendations for the management of the Helicobacter pylori infection have been developed. However, there has been no clear therapeutic regimen for the optimal treatment of Helicobacter pylori infection. This study was desinged to evaluate the eradication rate and the ulcer healing rate of the triple regimen with low-dose clarithromycin(0.5g/day) in peptic ulcer patients. Forty three patients with gastric ulcer(n=15) and duodenal ulcer(n=28) were enrolled. The patients were treated either with omeprazole 20mg b.i.d. amoxicillin 0.5g b.i.d. and clarithromycin 250mg b.i.d. (low-dose regimen group, n=29) or with omeprazole 20mg b.i.d., amoxicillin 1.0g b.i.d. and clarithromycin 0.5g b.i.d.(high-dose regimen group, n= 14) for 2 weeks. After 4 weeks, the patients were evaluated for the eradication of Helicobacter pylori and the healing of ulcer. And gastritis scores according to the Sydney system were evaluated before and after 4 weeks from the end of the triple regimen. There was no significant difference in the eradication rate and the ulcer healing rate between the low-dose regimen group, group and the high-does regimen group. In the low-does regimen group, the mean initial scores of chronic inflammation and activity were decreased after the eradication of Helicobacter pylori. In conclusion, the low-dose triple regimen is as effective as the high-dose triple regimen on the eradication of Helicobacter pylori and the ulcer healing rate in peptic ulcer disease.