Background study of NaI(Tl) crystals for the KIMS-NaI experiment

Adhikari, P,Adhikari, G,Choi, S,Ha, C,Hahn, I S,Jeon, EJ,Joo, H W,Kang, W G,Kim, H J,Kim, H O,Kim, K W,Kim, N Y,Kim, S K,Kim, Y D,Kim, Y H,Lee, H S,Lee, J H,Lee, M H,Leonard, D S,Li, J,Oh, S Y,Olsen, Institute of Physics Publishing Ltd 2016 Journal of Physics: Conference Series Vol.718 No.-

Ipriflavone의 흰쥐 뼈 결손부 재생 효과

김현진(HJ Kim),김옥준(OJ Kim),김대윤(DY Kim),정지연(JY Jung),김민석(MS Kim),김원재(WJ Kim),최홍란(HR Choi),이은주(EJ Lee),김선헌(SH Kim) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.2

Ipriflavone (isopropoxyisoflavone)은 soy isoflavone daidzein으로부터 합성된 isoflavone 일종으로 osteoporosis나 osteopenia 등에 이용되고 있다. 그러나 ipriflavone이 일차적으로 뼈 흡수를 억제함으로써 뼈 질환 치료에 임상적 효과를 갖는지 또는 뼈 형성을 촉진하는지에 대하여는 확실하지 않다. 또한 임상적 효능에 대한 효과가 대부분 골다공증의 치료 등에 국한되어 있는 반면, 단백 수준의 뼈 형성 인자의 유도 효과나 정상 뼈의 재생 효과를 밝히는 연구는 드물다. 본 연구는 in vitro에서 ipriflavone에 의한 뼈형성인자 발현에 대한 영향을 구명하고, 이를 생체에 국소적으로 직접 적용함으로써 ipriflavone의 뼈 재생 효과를 구명하고자 시도되었다. 시험관내 실험 : Ipriflavone 처리 3일 후 MC3T3-E1 뼈모세포주의 세포증식 효과는 10-8 M에서 10-4 M까지 대조군과 유 의한 차이가 없었다. 뼈 형성 지표로 알려진 collagen Iα1(I) 유전자 발현은 10-8 M에서 10-5 M까지 모든 농도에서 증가하였고 10-6 M에서 가장 많이 발현되었다. 그러나 10-4 M과 같이 높은 농도에서는 다시 크게 감소하여 대조군과 큰 차이를 보이지 않았다. alkaline phosphatase 발현은 대조군과 vehicle군과 같이 낮은 농도에서 큰 증가를 보이지 않았으나 10-5 M 농도에서 크게 증가하였다. 생체 실험 : Ipriflavone 투여 3일군에서 뼈 결손부는 대부분 혈병으로 채워져 있었고 혈병내에는 많은 혈액세포가 산재 하였으며, 실험군에서 대조군에 비하여 섬유원이 풍부하게 존재하였다. 약제 투여 7일 후 대조군과 실험군에서 모두 뼈 결손부 중심은 부분적으로 신생뼈에 의하여 재생되는 소견을 보였으며 대조군의 신생뼈는 풋뼈조직으로 구성되어 있었으 나, 실험군의 신생뼈는 일차해면뼈 조직으로 구성되어 있었다. 약제 투여 14일군에서 뼈 결손부가 뼈잔기둥 형태의 신생 뼈에 의하여 대치되어 있었으며 대조군에 비하여 실험군에서 더 많은 뼈조직으로 재생되어 있었다. 상기 연구 결과는 ipriflavone이 적정 농도에서 뼈 형성에 기여하며 뼈 결손부 재생 치유에 유용하게 사용될 수 있음을 시사한다. Ipriflavone (isopropoxyisoflavone) is a synthetic derivative from soy isoflavone daidzein. There are compelling evidences that it has an inhibitory effect on bone resorption in vitro and in vivo. It is not clarified however, whether its anti-osteopenic effect derives from inhibition of bone resorption or stimulation of bone formation. The present study aims to elucidate effects of ipriflavone on osteoblasts gene expression and possible clinical application on bone regeneration. Three days’ continuous treatment of ipriflavone had no effects on MC3T3-E1 osteoblastic cell proliferation at 10-8M to 10-4 M concentration from MTS assay. Gene expression of collagen α1(I), which is a bone formation marker, was markedly increased by ipriflavone treatment at 10-9 M to 10-5 M, but decreased to control level at 10-6 M concentration. Alkaline phosphatase, another bone formation marker, was also increased at its gene expression at 10-5 M concentration. Artificial bone defects on the rat calvaria were healed with hematoma and much more profuse and delicate fibrin at 3 days treatment of ipriflavone. Primary spongy bone was noted in bony defect center after 7 days treatment of ipriflavone, in contrast to woven bone in control group. Newly formed trabecular bone could be attained by the treatment after 14 days. These results suggested that ipriflavone can affect on osteoblasts at molecular level, and be clinically applied for local bone regeneration.

Discovery of Cathepsin K Inhibitors Using Fluorogenic Assay

NC Kim,YH Jung,JH Lee,HJ Hwang,YA Shin,SJ Lee,CH Lee,BY Lee,JW Lee,EJ Bae,MK Kim,MH Son,HT Shin,KJ Lim,SH Kim,WB Kim 대한약학회 1999 大韓藥學會 總會 및 學術大會 Vol.48 No.-

여러가지 형태의 난관결찰 부인에서 고정대를 이용한 미세난관문합수술

김장흡,김수평,김진홍,임용택,김은중,정기욱,권동진,고영미 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.6

저자들은 고정대를 이용한 미세난관복원수술 후 난관의 소통성과 여러가지 난관복원수술에 영향을 미치는 요인들에 따른 임신율을 알아보고져 1988년 3월부터 1994년 2월까지 가톨릭 의과대학부속 성모병원 산부인과 불임크리닉을 내원하여 고정대를 이용한 난관복원수술을 받았던 285예중 1년이상 추적이 가능했던 110예를 분석하여 다음과 같은 결론을 얻었다. 1. 난관복원술 당시의 환자의 평균 연령은 30.4±1.3(24~39)세 였으며 불임수술 후 복원수술까지의 기간은 평균 6.5±2.7(1~16)년 이었다. 2. 과거에 시술 받았던 불임수술의 종류중 복강경 시술이 88.2%로 대부분을 차지하였고 그중 전기소작법이 47.3%, ring을 이용한 난관결찰술이 40.9%였다. 3. 시행된 난관문합술의 부위별 분포는 협부-팽대부 문합이 49.1%로 가장 많았으며 그 다음으로는 협부-협부 문합이 36.4%로 많았다. 4. 고정대를 이용한 난관복원수술 후 전체적인 임신율은 80.0%였고 그 결과를 보면 만삭분만이 40.9%, 현재 진행중인 임신이 32.8%, 자연유산이 4.5%, 자궁외임신이 1.8%였다. 5. 불임시술방식에 따른 임신율과 복원수술 후 난관길이를 보면 복강경에 의한 ring삽입의 경우 각각 93.3%와 7.3±1.2cm로 전기소작술의 69.2%와 5.1±1.3cm보다 통계적으로 의의있게 높고 길었다. 6. 난관문합 부위에 따른 난관소통성과 임신율은 협부-협부 문합에서 각각 100.0%와 90.0%로 가장 높았다. 7. 임신이 된 군의 복원수술 후 평균 난관 길이는 6.6±1.5cm으로 임신이 안된 군의 4.4±1.6cm보다 통계적으로 의의있게 길었다. 8. 복원수술 후 양쪽 난관의 길이가 모두 4cm이상인 경우, 한쪽만 4cm 이상인 경우, 양쪽 모두 4cm이하인 경우의 순으로 임신율이 통계적으로 의의있게 높은 것으로 나타났다. 9. 고정대 삽입기간(3일 또는 7일)에 따른 난관소통성과 이신율의 차이는 없었다. 10. 복원수술 당시의 월경주기(중식기 또는 분비기)에 따른 임신율의 차이는 없었다. 이상의 결과로 이미 저자들에 의한 가토 난관에서 실험결과 고정대의 잔존이 난관의 점막세포 및 난관의 운동성에 영향을 주지 않고 난관의 소통성을 높여준다는 사실이 확인된 바와 같이 영구피임 목적으로 여러가지 형태의 난관결찰 수술을 받은 부인들에서 난관복원 수술시 1주일 동안의 고정대 삽입은 난관의 소통성과 임신율을 증대시킬 뿐만 아니라 문합부위의 노출을 좋게하여 문합수술의 시간을 단축시켜 수술 후 입원기간의 단축에도 많은 도움이 될 것으로 생각된다. Many methods have been tried to raise the rate of success of tuboplasty. The purpose of tuboplasty is to maintain tubal patency, to prevent postoperative adhesion, and to prevent abortion and tubal pregnancy for term delivery. Various kinds of tubal splints have been used to maintain the tubal patency after tubal anastomosis of experimental animals and human. Therefore, this investigation was performed to examine the clinical effectiveness of the Nylon splint for women who had underwent various types of tubal ligation and wanted tubal reversal. For target of study, 110 splinted women among 285 women who had underwent the tubal reversal using splint at Department of Obstetrics and Gynecology in St.Mary`s Hospital affiliated to Catholic University Medical College from Mar. 1989 to Feb. 1995 were selected and could be traced more than one year. The were assorted by tubal ligation method, tubal length after anastomosis, splint duration, menstrual phase at the time of operation, age and postoperative hysteroalpingopragphy. The results were as follows; 1. The mean age was 30.4 years and 88.2% of them had been sterilized by laparoscopic ring or cauterization. 2. The most common site of anastomosis was isthmic-ampular portion(49.1%). 3. The overall pregnant rate was 80.0% and the outcome of the pregnancy were as follows; term pregnancy(40.9%), on going pregnancy(32.8%), spontaneous abortion(4.5%), ectopic pregnancy(1.8%). 4. The mean pregnancy rate and postoperative tubal length of women who received the tubal ligation by laparoscopic ring were(93.3%, 7.3cm), statistically significantly higher and longer than that of the women by laparoscopic cauterization(69.2cm, 5.1cm). 5. The postoperative tubal patency patency and pregnancy rate of isthmic-isthmic anastomosis were the higher rate(100%, 90%). 6. The mean postoperative tubal length of pregnant group(6.6cm) was statistically significantly longer than that of non-pregnant group(4.4cm). 7. No statistical differences were found in pregnancy rates according to the time of splint removal(3 days or 7 days after operation) and menstrual phase at that time of operation.

ST1571 치료 후 완전 분자생물학적 관해가 증명된 가속기 만성골수성백혈병

김은정,장대영,안진석,이영경,조현찬,김유리,김동욱 大韓血液學會 2003 大韓血液學會誌 Vol.38 No.3

Electrophysiological safety of sibutramine HCl

Kim, KS,Park, SJ,Lee, HA,Kim, DK,Kim, EJ Sage Publications 2008 Human & experimental toxicology Vol.27 No.7

<P>Sibutramine is known to induce cardiovascular side effects such as tachycardia, vasodilation, and hypertension. The present study was aimed to examine the effects of sibutramine on action potential of guinea pig papillary muscle, recombinant hERG currents (I<SUB>hERG</SUB>), and inward currents (I<SUB>Na</SUB> and I<SUB>Ca</SUB>) of rat ventricular myocytes. Sibutramine at 30 μg/mL induced a shortening of action potential duration (APD) of guinea pig papillary muscle; on average, APD<SUB>30</SUB> and APD<SUB>90</SUB> were shortened by 23% and 17% at a stimulation rate of 1 Hz, respectively. Sibutramine suppressed the following currents: I<SUB>hERG</SUB> (IC<SUB>50</SUB>:2.408 ± 0.5117 μg/mL), L-type Ca current (IC<SUB>50</SUB>:2.709 ± 0.4701 μg/mL), and Na current (IC<SUB>50</SUB>:7.718 ± 1.7368 μg/mL). Sibutramine blocked I<SUB>hERG</SUB>, I<SUB>Ca</SUB>, and I<SUB>Na</SUB> in a concentration-dependent manner. In conclusion, sibutramine exerted a shortening effect on APD in guinea pig papillary muscle through its more powerful blocking effects on I<SUB>Ca</SUB> and I<SUB>Na</SUB> rather than I<SUB>hERG</SUB>.</P>

토끼난관 미세문합수술에서 문합부의 성장인자 (TGF) 발현의 비교

김장흡,이진우,김수평,김진홍,김은중,정기욱,권동진,노승혜 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.11

Twenty seven rabbits were divided into 3 groups of 1, 2-1 and 2-2(n=9 in each group). Arbitarilly cut ends of mid-ampullar portion of each tubes were microscopically reanastomosed using vicryl as suture material without intraluminal splint in group 1, using vicryl as suture material with intraluminal splint in group 2-1 and coagulation by Nd-YAG Laser beam as ref-usion method with intraluminal splint in group 2-2. A week after operation, the tubal tissue segments of reanastomosis were embedded into paraffin to examine the expression rate and intensity of TGF-β1, 2 and 3 by immunohistochemical staining. The results were as follows. 1. The expression rate of TGF-β1, 2 and 3 revealed no stastically significant difference between group 1 and 2. 2. Among comparisons group 2-1 and 2-2, the TGF-β1 expression rate in group 2-2 showed lower value than that in group 2-1 with stastical significance(P$lt;0.05). The above fact that tuboplasty with Nd-YAG Laser using intraluminal splint resulted in lower expression rate of TGF β1 may suggest raising the succe ss rate of pregnancy.

자궁경부암 환자에서 혈청내 진단 물질로서 in vitro translated HPV-16 E7 단백질과 GST-fusion HPV-16 E7 단백질의 임상적 효용성 비교

김승조,남궁성은,박종섭,김은주,김찬주,박순희,박동춘,김태연,엄수종 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.7

이상의 결과로서 자궁경부암 환자에서 in vitro translated HPV-16 E7 단백질과 GST-fusion HPV-16 E7 단백질의 임상적 효용성을 비교하여 볼 때 자궁경부암 환자에서 HPV-16 E7 항원에 대한 항체 측정이 질환의 진단을 예측할 수 있는 보조적인 인자로서 도움을 줄 것이며, 특히 in vitro translated HPV-16 E7 단백 항원은 재발의 예측 및 치료 평가에 효과적인 종양표지물질로 이용될 수 있으리라 생각된다. 이와 같이 직접적으로 임상적인 상태를 반영할 수 있는 자궁경부암을 유발시키는 HPV 단백질의 개발 연구와 그들의 임상적인 도입은 자궁경부암의 임상적 진단과 추적 관리에 큰 기여를 할 수 있을 것으로 보이며, 향후 자궁경부암과 연관된 연구에 기본적인 자료로서 가치가 있을 것으로 사료된다. Objective: Recently, a variety of HPV-related proteins have been synthesized and their utility as diagnostic and prognostic markers in cervical cancers is needed. The ability to generate preparative amounts of HPV-16 E7 proteins may have implications for the development of a serologic assay to detect anti-HPV-16 virion immune responses. The purpose of the study is to improve the way of proper management of the cervical cancer by investigating the clinical utility of in vitro translated HPV-16 E7 protein and GST-fusion HPV-16 E7 protein as the serologic marker through comparing the antibody reactions in the sera of the patients. Methods: The serologic responses were investigated in Korean women with cervical neoplasia by radioimmunoprecipitation assay (RIPA) using in vitro translated HPV-16 E7 protein and ELISA using GST-fusion HPV-16 E7 protein. PCR using E6 type-specific primers for HPV-16/18 were used to determine the presence and type of HPV infection (normal controls; 15 cases, preinvasive lesions; 15 cases, and invasive cervical cancers; HPV-16 DNA positive 78 cases and HPV-16 DNA negative 22 cases). Results: The sera of 39% (39/100) of patients with cervical cancer were RIPA positive for in vitro translated HPV-16 E7 protein [HPV-16 DNA positive cases; 41% (32/78), HPV-16 DNA negative cases; 32% (7/22)]. The sera of 29% (29/100) of cervical cancers were ELISA positive for HPV-16 GST-fusion E7 protein [HPV-16 DNA positive cases; 33% (26/78), HPV-16 DNA negative cases; 14% (3/22)]. By the two different methods, 12 cases were positively matched and 32 cases were negatively matched. The positivities for in vitrotranslated HPV-16 E7 protein in the patients with cervical cancer were 14% (7/49) in stage I, 59% (26/44) in stage II, and 86% (6/7) in stage III/IV, by significantly correlating with the advancing stage of the disease (P<0.01). Conclusions: These data suggests that the considerable number of patients with cervical neoplasia generated positive antibody response to in vitro translated protein and GST-fusion protein of HPV-16 E7. In contrast to RIPA method using in vitro translated protein, ELISA method using GST-fusion protein has several advantages for clinical application; use of non-isotope, easy preparation to produce a sufficient of protein, available large scale study. But the positive rates to in vitro translated HPV-16 E7 protein in the patients of cervical cancer were higher in general than those to GST-fusion HPV-16 proteins. Furthermore, the antibody positivities to in vitro translated HPV-16 E7 protein were increased by advancing clinical stage of disease. These HPV-16 E7 proteins might be the disease-specific markers which could be useful in adjunctive diagnostic assay and seroepidemiologic study of HPV-related cervical neoplasia.

자궁경부암의 광범위 자궁적출술 후 합병증 발생에 대한 임상적 고찰

김승조,송승규,이헌영,남궁성은,김수평,김은중,김사진,이귀세라,정선안 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.10

From March 1, 1991 through December 31, 1994, 87 patients with cervical carcinoma were treated with radical hysterectomy in the Department of Obstetrics and Gynecology, Catholic university Medical College, Holy Family Hospital. We studied about the duration of Hemovac insertion, the amount of Hemovac drainage, the duration of indwelling foley catheter, the amount of intraoperative or postoperative trasfusion, and postoperative complications among 87 patients who were operated at Holy Family Hospital. The results were as follows; 1. The mean age of patients was 50.5 years (range: 30 years-71 years). Patients in stage Ib were 49.5%. 2. Only radical hysterectomy without chemotheraphy or radiation theraphy was carried out 84.6% in stage Ia, 48.8% in stage Ib, 33.3% in stage Iia and 21.0% in stage IIb. 3. The mean duration of Hemovac insertion was 8.9 days(range: 2days-22days) and the mena amount of Hemovac drainage was 191.7 cc(range: 19.2 cc-1070.7 cc) The Hemovac was removed out when the drainage amount was about 70-80 cc. 4. The mean duration of indwelling foley catheter was 25.5 days (range; 14days-97days). 5. The mean amount of intraoperative or postoperative transfusion was 4.7 pints(range: 1pint-11 pints). 6. Among the postoperative complications, urinary tract infection was 18.4%, wound infection was 4.6%, stump abscess was 3.5%, postoperative bleeding was 1.2%, recurrent cervical carcinoma was 5.8%, and obturator nerve palsy, ureterovaginal and rectovaginal fistula were respectively 1 case.

임신과 동반된 중증 근무력증 1 례

김재욱,김세광,남궁란,최형민,이창희,이치형,안은주,김정년,선우일남 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.11

Mysathenia gravis is an autoimmune neuromuscular disorder maminfested clinically as weakness and easy fatigability of the skeletal muscle groups. It is caused by interference in the conduction of nerve impulses across the myoneural junction due to antibodies directed against the acetylocholine receptor. During pregnancy, the clinical course of myasthenia gravis is usually not a steady state, and influences of myasthenia gravis on pregnancy outcome is variable. We experienced a patient with myasthenia gravis associated with pregnancy who underwent a vaginal delivery and transient neonatal myasthenia gravis of the newborn. We present this case and with a review of the pertinent literature.