肉類의 焙燒 방법에 따른 N-nitrosamine 含量에 關한 硏究

오명철,오창경,김수현 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

소갈비, 돼지갈비 및 통닭을 가스렌지와 숯불로 焙燒한 후 배소 전후의 N-nitrosamine과 그의 전구물질인 TMAO, TMA, DMA, nitrate 및 nitrite의 함량을 측정하였다. TMAO-N, TMA-N 및 DMA-N은 배소 전후 모든 시험구에서 뚜렷한 변화가 없었으며, 그 함량은 각각 N.D∼0.76 mg/kg, N.D∼2.57mg/kg 및 0.02∼0.60mg/kg 범위였다. 배소 후 nitrate-N는 감소하였고, nitrite-N는 증가하였으며, 그 함량은 각각 4.0∼6.7mg/kg 및 0.7∼3.2mg/kg 범위였다. N-nitrosamine은 배소 후에 NDMA가 5.9∼20.9 ㎍/kg, NDEA가 0∼3.0㎍/kg으로 검출되었다. The contents of N-nitrosmines and its precursors such as TMAO-N, TMA-N, DMA-N, nitrite-N and nitrate-N after broiling of beef-ribs, pork-ribs and chicken upon gas range and charcoal fire were investigated. The contents of TMAO-N, TMA-N and DMA-N no changed in all tested meats before and after broiling, and were in the range of N.D∼0.77mg/kg, N.D∼2.57mg/kg and 0.02∼0.60mg/kg, respectively. Nitrate-N discreased and Nitrite-N increased after broiling, and the contents were in the range of 4.0∼6.7mg/kg and 0.7∼3.2mg/kg, respectively. N-nitrosamines detected NDMA and NDEA in the broiled meats, and the contents were in the range of 5.9∼20.9㎍/kg and 0∼3.0㎍/kg respectively.

아시아 지역 폐렴 구균의 항균제 내성 현황 및 변화 양상 : Asian Network for Surveillance of Resistant Pathogens (ANSORP) Study

정숙인,김나영,손준성,기현균,고관수,서지연,장현하,김연숙,오원섭,백경란,이남용,송재훈,항생제 내성 감시를 위한 아시아 연합 (ANSORP) 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

목적 : 1980년대부터 페니실린을 비롯한 β-lactam 계열과 비 β-lactam 계열 항균제에 대한 폐렴구균의 항생제 내성이 급격하게 증가하여 국내 뿐 아니라 세계적인 문제로 대두되고 있다. ANSORP 에서는 아시아 지역에서 폐렴구균의 항균제 내성과 그 변화 추이, 내성의 기전 및 위험인자를 분석하고자 본 국제 공동 연구를 시행하였다. 재료 및 방법 : 1999년 11월부터 2001년 8월까지 아시아 지역 12개국의 14개 기관에서 폐렴구균 감염증 환자의 임상 검체로 분리된 침습성 폐렴구균을 대상으로 액체배지 미량희석법을 이용하여 16개 항생제의 MIC를 구하였다. 위험인자의 분석을 위하여 폐렴구균 감염증 환자를 대상으로 의무기록지를 조사하였다. Quellung 반응을 통해 혈청형을 결정하였다. 결과 : 총 685균주 중 52.4%가 페니실린에 대하여 비감수성(중등도 내성 22.9%, 내성 29.5%)을 보였고, 그 빈도는 베트남(92.1%), 스리랑카(85.7%), 홍콩(67.9%), 한국(64.5%)의 순이었다. Erythromycin에 대한 비감수성 비율은 베트남(92.1%), 대만(87.7%), 한국(80.6%), 홍콩(76.8%) 등에서 높게 나타났고, 베트남, 대만, 한국, 홍콩 등의 erythromycin의 MIC_(90)이 32㎍/mL를 넘는 고도내성을 보였다. 홍콩, 싱가포르, 대만, 인도에서는 fluoroquinolone에 대한 내성 균주가 출현하였다. 전체 균주 중 다제내성 균주의 비율은 47.7%였다. 혈청형은 19형과 23형이 가장 많았고, 그 외 6형, 14형 등이 있었다. 페니실린 비감수성 감염증의 위험인자로는 5세 미만(OR=1.7; 95%, CI, 1.2-2.4; P=0.002), 기관지폐질환(OR=2.0; 95%, CI, 1.3-3.1; P=0.003), 악성종양(OR=2.3; 95%, CI, 1.2-4.6; P=0.015), 스테로이드의 사용(OR=2.8; 95%, CI, 1.1-7.4; P=0.032)이 확인되었다. 결론 : 침습성 폐렴구균 균주를 대상으로 한 본 연구의 결과 아시아 국가들에서 페니실린, β-lactam 및 macrolide에 대한 폐렴구균의 내성률 및 내성 정도가 급증하고 있음을 확인하였다. Background : Emergence of pneumococcal resistance became a global issue since 1990s. According to the ANSORP studies with clinical isolates and carriage isolates between 1996 and 1999, some Asian countries showed alarmingly high prevalence of resistance to penicillin and other antimicrobial agents. To investigate the changing trends of pneumococcal resistance, ANSORP study group has performed a multinational surveillance study with invasive pneumococcal isolates from Asian countries. Methods : All isolates from various invasive pneumococcal diseases were prospectively collected from 14 centers in 12 countries between November 1999 to August 2001. Broth microdilution tests with 16 antimicrobial agents were performed according to the NCCLS procedures. Serotyping was performed by means of Quelling reaction with use of group-specific antisera. Results : A total of 685 isolates were collected. Overall, 52.4% of invasive isolates from Asian countries were not susceptible to penicillin (intermediate (1), 22.9%, Resistant (R), 29.5%). Vietnam showed the highest prevalence of penicillin non-susceptibility (1 20.6%, R 71.4%) followed by Sri Lanka (1 71.4%, R 14.3%), Hong Kong (1 24.1%, R 76%) and Korea (1 9.7%, R 54.8%). China (I 19.8%, R 23.4%) and Malaysia (1 9.1%, R 29.5%) also showed remarkable increase in penicillin resistance compared with previous ANSORP data, which were less than 10%. Vietnam (92.1%), Taiwan (87.7%), Korea (80.6%), and Hong Kong (76.8%) showed high prevalence of erythromycin resistance. MIG_(90)S for ciprofloxacin were 4 ㎍/mL (Hong Kong) and 2 ㎍/mL (11 Asian countries except Hong Kong), respectively. Conclusion : Compared with previous data from ANSORP studies, antimicrobial resistance among invasive pneumococcal isolates has markedly increased in Vietnam, Sri Lanka, Taiwan, China, and Malaysia. Continuous surveillance of pneumococcal resistance in Asia is strongly warranted.

췌장염에 의한 가성낭종내에 생긴 가성동맥류 : 1례 보고

오연희,이채경,김승현,이성우,양창현,이정호,이영현 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

만성 췌장염의 합병증으로 생긴 가성동맥류는 드문질환으로, 반복적으로 다량의 출혈을 일으킬 수 있으며, 치료를 하지 않을 경우 사망율이 높기 때문에, 조기 진단과 치료가 필수적이다. 저자들은 38세 남자 환자에서 췌장염의 합병증으로 생긴 가성낭종내에서 발생한 가성동맥류 1예를 경험하였기에 보고하는 바이다. 가성 동맥류의 색혈류도플러상 낭종내에 양방향 흐름의 와류를 볼 수 있었다. Pancreatic Pseudoaneurysm within Pseudocyst from Pancreatitis: 1 Case Department of Diagnostic Radiology and Internal Medicine, College of Medicine,DongGuk University Yeon Hee OH M.D., Chae Kyeong Lee M.D., Seoung Hyeon Kim M.D., Sung Woo Lee M.D., Chang Heon Yang M.D., Jung Ho Lee M.D., Young Hyun Lee M.D. Pseudoaneurysm from pancreatitis is uncommon, but it can cause recurrent and massive hemorrhage. Because of high morbidity and mortality, early detection and treatment of the pseudoaneurysm is needed. We report a case of pseudoaneurysm within pseudocyst from pancreatitis. Color-flow Doppler sonography shows bidirectional flow and turbulent arterial flow, within anechoic mass.

오징어젓 숙성 중 N-nitrosamine 생성에 관한 연구

오창경,김수현,송대진,오명철,김성수 濟州大學校工科大學産業技術硏究所 1996 尖端技術硏究所論文集 Vol.7 No.1

본 연구는 오징어에 식염 20%를 첨가하는 고식염구와 식염 10%에 솔비톨, 에탄올, 젖산 등을 첨가한 저식염구로 나누어 오징어젓을 제조한 후, 이들을 숙성시키면서 N-nitrosamine의 생성 여부와 숙성 중 그의 전구물질인 아질산염과 질산염, DMA 및 TMA와 TMAO의 변화를 검토하였다. 오징어젓 숙성 중 pH는 저식염구가 5.79∼6.07, 고식염구가 6.05∼6.21 사이에서 변화하였다. 아질산염질소와 질산염질소는 각각 0.75∼1.04mg/kg 및 1.22∼1.42mg/kg 사이에서 변화하였다. TMA-N는 숙성 중 계속하여 증가하여 숙성 140일에는 고식염구가 20.1mg%, 저식염구가 14.9mg%였으며, TMAO-N는 생시료에서 27.9mg%이었던 것이 숙성 중기까지 완만하게 증가하다가 그 이후에는 다시 감소하였다. DMA-N는 숙성 중 계속하여 증가하였으며 숙성 140일에는 고식염구는 19.7mg/kr, 저식염구는 13.3mg/kg이었다. 오징어젓의 N-nitrosamine으로는 NDMA만이 검출되었고, 숙성 40일째에 저식염구에 흔적량, 고식염구에 3.8㎍/kg의 양으로 생성되었으며 숙성 중 계속해서 증가하여 57.5∼74.7㎍/kg에 이르렀다. Possibility of formation of carcinogenic N-nitrosamines and changes in their precursors during the fermentation of high salt-fermented squeid, Sepiell maindroni, group with 20% of salt and low salt-fermented squid group with 10% of salt, 6% of ethanol, 6% of sorbitol and 0.5% of lactic acid were investigated. pH during the fermentation of squid changed in the ranges of 5.79∼6.07 and 6.05∼6.21 in low and high salt group, respectively. The contents of nitrite- and nitrate-N during the fermentation were in the ranges 0.75∼1.04 and 1.22∼1.42mg/kg, respectively. Trimethylamine(TMA) was continuously increased during the fermentation and showed 20.1 and 14.9mg% in high and low salt group after 140 days of the fermentation, respectively, while trimethylamine oxide(TMAO) was gradually increased from the beginning until the middle period of fermentation and then was again decreased. Dimethylamine(DMA) was continuously increased during the fermentation and showed 19.7 and 13.3mg/kg in high and low salt group after 140 days of the fermentation, respectively. N-nitrosamines in salt-fermented squid was detected only N-nitrosodimethylamine(NDMA), which its amount was trace in low salt group and 3.8㎍/kg in high salt group on 40 days of the fermentation and then continuously increased in the ranges of 57.5∼74.7㎍/kg in both groups during the fermentation.

Add-on Effect and Safety of Pharmacopuncture Therapy in the Treatment of Patients with Lumbar Spinal Stenosis

Oh Yoona,Han Chang-Hyun,Kim Yeonhak,Kim Jihun,Yang Changsop,Choi Young Eun,Kang Byoung-Kab,Yang Gi Young,Lee Byung Ryul,Kim Eunseok 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.1

Background: Recently, Korean Medicine treatment with pharmacopuncture therapy (PPT) has been increasingly used in clinical practice to improve symptoms in patients with lumbar spinal stenosis (LSS). The aim of this study is to evaluate the effectiveness and safety of PPT in addition to conventional Korean Medicine treatment (CKMT) for the treatment of patients with LSS, compared with CKMT alone. Methods: This study is designed as a pragmatic, randomized, two-armed, parallel, stratified (by sex), controlled pilot trial. Forty patients diagnosed with LSS will be randomly allocated to the PPT + CKMT group or the CKMT group. Patients in the two groups will receive treatment two times weekly for 5 weeks. The primary outcome will be the mean change in the 100-mm visual analog scale score from the baseline to the end of treatment (week 5). The secondary outcomes will include the clinically important difference, Zurich Claudication Questionnaire score, self-reported walking capacity, Modified–Modified Schober test, EuroQol 5-dimension 5-level questionnaire, and Patients’ Global Impression of Change. Adverse events will be assessed at each visit. Discussion: The results of this study will provide meaningful data to evaluate the add-on effect and safety of PPT in the medical care of patients with LSS.

고등어 염장중 N-Nitrosodimethylamine 생성 및 그 전구물질들의 변화

金洙賢,吳昌璟 제주대학교 1993 논문집 Vol.36 No.-

여러 식품에서 아질산염과 제2급 및 제3급 아민 또는 제4급 암모니윰 화합물과의 상호작용에 의해 형성 되어지는 N-nitrosamine은 상당한 관심의 대상이 되고 있다. 본 연구는 5℃에 저장된 염장고등어에서 발암성 N-nitrosamine의 생성과 이의 전구물질인 DMA-N, TMA-N, betaine-N choline-N 및 총 creatinine의 변화를 10일 주기로 조사하였다. 또한, Ames의 표준평판 혼성시험법의 변법인 예비배양법을 이용하여 NDMA의 돌연변이유발 활성을 검정하였다. 본 연구에서 얻은 결과를 요약하면 다음과 같다. 1) pH는 10% 및 20% 염장품이 각각 5.62-5.73과 5.68-5.93으로 N-nitrosamine 생성 최적 pH보다 높은 범위에서 변화하였다. 수분은 생시료에 비해 훨씬 낮은 양으로 변화하였다. 수분은 생시료에 비해 훨씬 낮은 양으로 변화하였다. 염도는 각각 저장 20일 경과후에 5.7 및 11.4%이었고, 이 후 서서히 증가하여 저장 말기에는 각각 7% 및 13%의 염분침투가 이루어 졌다. V.B.N.은 저장초기에 급격히 증가하였으며 전반적으로 V.B.N.은 10%가 20% 염장품보다 약간 높은 함량 변화를 보였으나, 어류의 초기 부패치인 30mg/100g을 초과하지 않은 것으로 보아 저장기간 전반에 걸쳐 부패가 진행되지 않았음을 알 수 있었다. 2) 총 creatinine-N과 betaine-N 등의 제4급 암모니윰 화합물은 저장 중기에 상당히 증가하였으나, 저장 중기 이후에는 감소한 반면, cholone-N는 저장중에 조금씩 계속 증가하였다. 3) TMAO-N와 TMA-N 함량은 저장 초기에 변화가 심하였으나, 대체적으로 TMAO-N는 증가하고 TMA-N는 감소하는 역상관관계를 보였다. 4) DMA-N 함량은 저장중에 계속 증가하였으며, 10%과 20% 염장품간의 차이는 거의 없었다. 5) NDMA는 10% 및 20% 염장품이 각각 저장 20일 및 30일 경과시부터 검출되었으며 그 함량은 극히 낮았다. 반면, 아질산염 첨가구의 경우, 비첨가구 보다 약 500배 낮은 함량 변화를 보였으며, 대체적으로 저장기간중에 조금씩 증가하였다. 6) 돌연변이유발 활성은 plate당 1㎍(10ppm)의 NDMA를 첨가한 TA100 균주에서 가장 높게 나타났다. 결론적으로, 고등어 염장중 NDMA 생성 가능성은 아질산염과 DMA간의 상호작용에 의해서는 물론, 아질산염과 제3급 amine 및 제4급 ammonium염과의 상호작용에 의해 영향을 받으며, 저염 및 고염 염장품의 차이는 거의 없는 것으로 나타나기 때문에 저염고등어 제법을 개발하였을 때 NDMA 생성과는 무관하다고 판단되었다. NDMA의 돌연변이유발 활성은 10-100ppm의 농도에서 나타났다. The possibility of formation of carcinogenic N-nitrosodimethylamine and changes of their precursors in salted mackerel, Scomber japonicus, stored at 5℃ were periodically investigated, and changes of precursors between low (10%) and high (20%) salted mackerel was compared. The results were as follow; 1) Changes of pH were in the ranges of 5.62-5.73 and 5.68-5.93 in 10% and 20% salted mackerel during their storage, respectively. 2) Moisture changes in very low contents in compared with fresh sample. 3) Salinity gradually increased in low and high salted mackerel and salt permiation of 7 percent and 13 percent was accomplished during storage of both groups, respectively. 4) The contents of total creatinine and betaine remarckably increased at the middle of storage, while choline contents was scarcely changed. 5) The contents of trimethylamine oxide and trimethylamine severely varied at the beginning of storage. Changes of contents between TMAO and TMA showed, apparently, reverse relationship. 6) The contents of dimethylamine showed nearly same increasing pattern and continuously increased during storage in both groups. 7) In the low salted mackerel, N-nitrosodimethylamine was detected from 20 days of storage, while in the high salted mackerel, it was detected from 30 day of storage. In both salted mackerel added sodium nitrite, the contents of NDMA were more 500 times than those in both salted mackerel without sodium nitrite. In conclusion, it was found by this study that the possibility of formation of NDMA might be affected not only by the interaction between nitrite and DMA but also by the interaction between nitrite and TMA, TMAO, choline, betaine, and creatinine in salted mackerel during storage. No difference of the contents of precursors and NDMA between both Samples(10% & 20% NaCl) was nearly.

Protective effect of chorioamnionitis on the development of bronchopulmonary dysplasia triggered by postnatal systemic inflammation in neonatal rats

Choi, Chang Won,Lee, Juyoung,Oh, Joo Youn,Lee, Seung Hyun,Lee, Hyun Ju,Kim, Beyong Il Nature Publishing Group 2016 Pediatric research Vol.79 No.2

<P><B>Background:</B></P><P>Prenatal or postnatal systemic inflammation can contribute to the development of bronchopulmonary dysplasia (BPD). We investigated whether prenatal intra-amniotic (i.a.) inflammation or early postnatal systemic inflammation can induce BPD in a rat model.</P><P><B>Methods:</B></P><P>One microgram of lipopolysaccharide (LPS) or vehicle was injected into the amniotic sacs 2 d before delivery (E20). After birth, 0.25 mg/kg of LPS or vehicle was injected into the peritoneum of pups on postnatal day (P)1, P3, and P5. On P7 and P14, peripheral blood (PB), bronchoalveolar lavage fluid (BALF), and lung tissue were obtained and analyzed.</P><P><B>Results:</B></P><P>Postnatal i.p. injections of LPS significantly increased neutrophil counts in PB and BALF on P7 and P14. Similarly, proinflammatory cytokine and angiogenic factor transcript levels were increased in the lung by i.p. LPS on P7. Alveolar and pulmonary vascular development was markedly disrupted by i.p. LPS on P14. However, pretreatment with i.a. LPS significantly negated the detrimental effects of postnatal i.p. LPS on PB and BALF neutrophil counts and on lung proinflammatory cytokine expression and histopathological changes.</P><P><B>Conclusion:</B></P><P>Exposure to early postnatal systemic LPS induces BPD, an arrest in alveolarization, in neonatal rats. Preceding exposure to i.a. LPS protects the lungs against BPD triggered by postnatal systemic inflammation.</P>

육류의 배소 방법에 따른 N-nitrosamine 함량에 관한 연구

김수현,오명철,오창경 濟州大學校 産業技術硏究所 1995 산업기술연구소논문집 Vol.6 No.-

The contents of N-nitrosamines and its precursors such as TMAO-N, TMA-N, DMA-N, nitrite-N and nitrate-N after broiling of beef-ribs, pork-ribs and chicken upon gas range and charcoal fire were investigated. The contents of TMAO-N, TMA-N and DMA-N no changed in all tested meats before and after broiling, and were in the range of N.D-0.77mg/kg, N.D- 2.57mg/kg and 0.02 - 0.06mg/kg, respectively. Nitrate-N discreased and Nitrite-N increased after broiling, and the contents were in the range of 4.0-6.7mg/kg and 0.7-3.2mg/kg, respectively. N-nitrosamines detected NDMA and NDEA in the broiled meats, and the contents were in the range of 5.9 ~ 20.9 ㎍/kg and 0 ~ 3.0 ㎍/kg respectively.

발열을 동반한 호중구감소증에서 Cefepime 단독투여와 Ceftazidime 및 Tobramycin 병합투여의 효능비교

정현욱,채제욱,강미라,양정채,문치숙,기현균,장현하,오원섭,김기현,백경란,이남용,송재훈 대한감염학회 2004 감염과 화학요법 Vol.36 No.6

목적 : 국내에서 발열을 동반한 호중구감소증이 있는 악성 종양환자에게 경험적 항생제로 베타락탐계 항생제와 아미노배당체의 병합요법의 사용이 일반적이다. Cefepime은 광범위 항균 작용을 가지고 있어, 그람 음성균 뿐만 아니라 그람 양성균에 대해서도 우수한 효과를 나타낸다. 재료 및 방법: 발열을 동반한 호중구감소증이 있는 악성 종양환자를 대상으로 무작위, 공개, 비교 연구를 시행하였다. 대상 환자를 무작위로 cefepime 단독요법군과 ceftazidime 및 tobramycin 병합요법군으로 나누어 투여하고 각각의 임상적 효능과 안전성을 비교하였다. 구강및 인후 점막염이 있는 환자에서 분리된 녹색 연쇄알 구균에 대한 항생제 내성 정도를 조사하였다. 결과 : 대상환자 89명 중 CA 투여군이 48예(53.9%), CT 투여군이 41예(46.1%)이었다. 발열의 유형별로 MDI는 18예(20.2%), CDI는 9예(10.1%), UF는 62예(69.7%)로 두 군 간에 차이가 없었다. CA 투여군과 CT 투여군의 임상적 호전률은 시험약 투여 후 2-4일째 각각 91.7%, 85.4% (P=0.31), 치료 종료 시 각각 91.7%, 100% (P=0.15)로 두 군간에 유의한 차이가 없었다. 치료 종료 시 CA 투여군과 CT 투여군의 세균학적 소실률은 모두 100%로 두 군간의 유의한 차이가 없었다(P=0.78). 점막염이 있는 환자로부터 녹색 연쇄알 구균이 분리된 경우는 25예(28.1%)이었으며, 분리된 녹색 연쇄알 구균은 penicillin, ceftriaxone, cefepime, vancomycin에 모두 감수성을 보였다. 약제 관련 이상 반응의 발생 빈도도 두 군간에 유의한 차이가 없었다. 결론 : 발열을 동반한 호중구감소증이 있는 악성 종양환자의 경험적 항생제로서 cefepime 단독요법은 ceftazidime 및 아미노배당체의 병합요법만큼 효과적이고 안전하였다. Background : Broad-spectrum antibiotic therapy has been recommended as an empirical regimen in cancer patients with febrile neutropenia. Cefepime is a fourth generation cephalosporin with good activity against both gram-positive cocci and gram-negative bacilli. Materials and Methods : To compare the efficacy and safety of cefepime alone with ceftazidime plus tobramycin as empirical regimen for adult cancer patients with febrile neutropenia, a randomized, open label, comparative trial was performed. If the patient showed clinical improvent 72 hours, antibiotic could be changed to oral ciprofloxacin. Clinical and microbiological responses were determined at 72 hours and at the end of therapy. To investigate the antimicrobial resistance of viridans streptococci, swab cultures were obtained from throat in all enrolled patients and antimicrobial susceptibility tests were performed by using microdilution method according to the NCCLS. Results : A total of 89 patients were enrolled. Forty-eight patients received cefepime alone (CA), and 41 patients received ceftazidime plus tobramycin (CT). Demographic and baseline clinical characteristics were similar in both groups (P>0.05). The initial clinical success rate at day 2-4 in group CA (91.7%) was similar with that in CT group (85.4%) (P=0.31). At the end of therapy, the final clinical success rate in CA group (91.7%) was similar to that in CT group (100%) (P=0.15). In 18 patients, with microbiologically defined infections, the eradication rate was 100% in both groups. Adverse events including liver dysfunction (21.3%) and renal dysfunction (2.2%), were similar in both groups (P=0.87). Viridans streptococci were isolated from the throat cultures in 25 cases, and all of these strains were susceptible to penicillin (MIC_(90) 0.12 ㎍/mL), cefepime (1 ㎍/mL), and vancomycin (0.12 ㎍/mL). Conclusion : Efficacy and safety of cefepime monotherapy was comparable to the combination of ceftazidime and tobramycin. It could be used as an alternative empirical regimen for treating cancer patients with febrile neutropenia.

오징어젓 숙성 중 제4급 암모늄 화합물의 변화

김성수,송대진,오명철,오창경,김수현 濟州大學校工科大學産業技術硏究所 1996 尖端技術硏究所論文集 Vol.7 No.1

The changes in precursors of N-nitrosamines, such as choline-N, betaine-N and total creatinine-N, during the fermentation of high salt-fermented squid, Sepiell maindroni, group with 20% of salt and low salt-fermented squid group with 10% of salt, 6% of ethanol, 6% of sorbitol and 0.5% of lactic acid were investigated. During the fermentation, the contents of VBN were rapidly increased from the beginning of the fermentation until 140 days, and these contents were more in high salt groups as compared with low salt groups. The contents of choline-N were little changed until 80 days of the fermentation and slowly decreased after 80 days of the fermentation to 120 days, but the different of the contents between both salt groups were little. Betaine-N also showed the similar tendency to choline-N, but the changes in low salt groups were slowly increased and decreased as compared with these in high salt group. Total creatinine-N was slowly increased from the beginning of the fermentation until 60 days in both salt groups, but rapidly decreased after this to 120 day.