Optimal Fermentation Conditions for Enhanced GlutathioneProduction by Saccharomyces cerevisiae FF-8

Jae-Young Cha1,,Jin-Chul Park,Beong-Sam Jeon,Young-Choon Lee,Young-Su Cho 한국미생물학회 2004 The journal of microbiology Vol.42 No.1

The influence of feedstock amino acids, salt, carbon and nitrogen sources on glutathione production by Saccharomyces cerevisiae FF-8 was investigated. Glucose, yeast extract, KH2PO4, and L-cysteine were found to be suitable feedstock. Highest glutathione production was obtained after cultivation with shaking for 72 h in a medium containing glucose 3.0% (w/v), yeast extract 3.0%, KH2PO4 0.06% and L-cysteine 0.06%. The glutathione concentration achieved using this medium increased 2.27-fold to 204 mg/l compared to YM basal medium.

Real-World National Colonoscopy Volume in Korea: A Nationwide Population-Based Study over 12 Years

Jae Myung Cha,Min Seob Kwak,Hyun-Soo Kim,Su Young Kim,Sohee Park,Geun U Park,Jung Kuk Lee,Soo Jin Kim,Hun Hee Lee,Joo Sung Kim,Won Ho Kim,Big Data Research Group of the Korean Society of Gastroenterol 거트앤리버 소화기연관학회협의회 2020 Gut and Liver Vol.14 No.3

Background/Aims: Little is known about the national colonoscopy volume in Asian countries. This study aimed to assess the national colonoscopy volume in Korea over a 12-year period on the basis of a nationwide population-based database. Methods: We conducted a population-based study for colonoscopy claims (14,511,158 colonoscopies performed on 13,219,781 patients) on the basis of the Korean National Health Insurance Service database from 2002 to 2013. The 12-year national colonoscopy burden was analyzed according to patient age, patient sex, and healthcare facility type. Results: The overall volume of colonoscopy increased 8-fold over the 12-year period. The annual colonoscopic polypectomy rate significantly increased in all patient sex and age groups over the 12-years period (all p<0.001). The yearly colonoscopic polypectomy rate for men was significantly increased compared with that for women (2.3% vs 1.7%, p<0.001) and for the screening-age group compared with that for the young-age group (2.0% vs 1.6%, p<0.001). The yearly colonoscopic polypectomy rate relative to the total colonoscopy volume significantly increased in primary, secondary, and tertiary facilities by 2.4%, 1.9%, and 1.4% during the 12-year period (all p<0.001). In addition, the annual colonoscopy volume covered by high-volume facilities significantly increased by 1.8% in primary healthcare facilities over the 12-year period (p<0.001). Conclusions: Healthcare resources should be prioritized to allow adequate colonoscopic capacity, especially for men, individuals in the screening-age group, and at primary healthcare facilities. Cost-effective strategies to improve the quality of colonoscopy may focus on primary healthcare facilities and high-volume facilities in Korea.

Comparative occurrence of ischemic stroke with the rhythm versus rate control strategy in a national prospective cohort of atrial fibrillation

( Jae Guk Kim ),( Young Soo Lee ),( Ki-woon Kang ),( Eue-keun Choi ),( Myung-jin Cha ),( Jung-myung Lee ),( Jin-bae Kim ),( Junbeom Park ),( Jin-kyu Park ),( Tae-hoon Kim ),( Jae-sun Uhm ),( Jaemin Sh 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.1

Background/Aims: Comparative occurrence of ischemic stroke for rhythm versus rate control strategy in patients with non-valvular atrial fibrillation (NVAF) is still inconclusive. The purpose of this study was to investigate whether the rhythm control strategy is associated with a lower risk of ischemic stroke compared to the rate control strategy in NVAF patients. Methods: The CODE-AF registry prospectively enrolled 6,280 consecutive patients who were treated for NVAF at 10 tertiary referral centers in South Korea. Of these, 2,513 NVAF patients (age, 67 ± 10 years; male, 61.8%) were clinically followed up for over 1-year and divided into rate and rhythm control groups. Results: Those treated with the rhythm control strategy were younger and had less proportions of underlying disease compared to those treated with the rate control strategy. After the propensity matching analysis, those treated with the rhythm control strategy had similar baseline characteristics including the CHA₂DS₂-VASC score compared to those treated with the rate control strategy. The rate of oral anticoagulation, all bleeding, and hospitalization were also similarly between the two groups. The incidence rate of ischemic stroke in the rhythm control group was significantly lower than in the rate control group (0.7 vs. 6.9 per 1,000 person-years, p = 0.011). Conclusions: The rhythm control strategy demonstrated a beneficial effect to lower the risk of ischemic stroke during a 1-year follow-up compared to the rate control strategy.

RFID 사용요인이 신뢰, 지각된 위험, 모바일RFID환경 하에서 사용의도에 미치는 영향 -약국RFID 시스템을 중심으로-

최재영 ( Jae Young Choi1 ),차재빈 ( Jae Bin Cha1 ),김상만 ( Sang Man Kim ) 경희대학교 경영연구원 2013 의료경영학연구 Vol.7 No.1

This study examined the influence of various factors of RFID use in mobile RFID environment, focusing on pharmacies` RFID systems. The purpose of the study was to verify the possibility of a more widespread use of RFID and mobile RFID in medical industry by exploring what impact RIFD use factors have on the intention to use in RFID environment. The subjects were pharmacists who have their own pharmacies in Seoul and Gyeonggi Province. Of 400 questionnaires distributed to the respondents, 392 were used in the final analysis except 8 with insufficient answers. The empirical analysis results showed that perceived security, perceived cost, and social influence had significant impacts on trust, while social influence and perceived cost had significant impacts on perceived risk. Among RFID use factors, social influence, perceived security, and perceived risk had significant impacts on intention to use in mobile RFID environment. Trust and perceived cost did not have a significant impact. The summary of analysis results, implications, and limitations are presented in the conclusion.

가스화 - 수소 회수 공정 개발을 위한 예비실험

차재준(Jae-Jun Cha),김영석(Young-Suk Kim),이진호(Jin-Ho Lee),황순모(Soon-Mo Hwang),정성재(Seong-Jae Jeong) 한국열환경공학회 2010 한국열환경공학회 학술대회지 Vol.2010 No.춘계

위암의 연령에 따른 차이

김영재(Young Jae Kim),이영철(Young Chul Rhee),이만길(man Kill Lee),차호(Ho Cha),정영채(Young Chae Jung) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1

N/A Comparative analytic review on clinical, endoscopic and histopathological findings was done in 13 cases of young age (under 29), 446 cases of mid age (30~69) and 39 cases of old age (above 70) stomach cancer who were visited Department of Internal Medicine of Masan Koryo General Hospital from March 1981 to July 1985. The following results were obtained: The peak of age incidence was the 5th decade amounting to 36.8%. The ratio between male and female was 2.6:1. The main chief complaint of each group was epigastric pain and it was followed by epigastric fullness. The most patient had the duration of symptom from 3 months to 6 months. For old-age group, mid-age group, major site of lesion in the antrum, meanwhile body for young-age group. The most frequent type of gastric cancer was type III in each group by Borrmanns classification. For each group, adenocarcinoma was major cases in the view of histopathological findings, and incidence of well differentiated type adenocarcinoma were lower in young-age group than others.

한국인 인슐린 비의존형 당뇨병 및 이차성 당뇨병 환자에서 글루코키나제 유전자 변이

남재현(Jae Hyun Nam),이현철(Hyun Chul Lee),김연의(Youn Euy Kim),권석호(Suk Ho Kwon),윤용석(Yong Suk Yoon),박석원(Suk Won Park),원영준(Young Jun Won),차봉수(Bong Su Cha),송영득(Young Duk Song),이은직(Eun Jig Lee),임승길(Sung Kil Lim) 대한내과학회 1998 대한내과학회지 Vol.54 No.6

N/A Objectives: Mutations in the glucokinase (GCK) gene are considered a possible cause of maturity-onset diabetes of the young. The purpose of this study was to evaluate the contribution of this gene to the development of non insulin dependent diabetes mellitus (NIDDM), gestational diabetes mellitus (GDM) and post-renal transplantation diabetes mellitus (PTDM). Method: Identification of GCK mutation was attempted on 39 NIDDM patients, 2 GDM patients and 58 selected renal allograft recipients with PTDM and 45 normal controls. The exons in the GCK gene were examined by polymerase chain reaction (PCR), followed by analysis of single-stranded DNA conformational polymorphism (SSCP). The abnormal bands were also confirmed by DNA sequencing analysis. The exons of affected family members were also investigated for mutations of the GCK gene. Results: Two of the 58 PTDM patients (3.4%) were found to have CKK mutations. One had the mutation on exon 5 and the other on intron 7. One control subject had the mutation on intmn 9. The mutation of exon 5 was identified as a substitution of CCT (proline) for CTT (leucine) at codon 164, which has not ever reported before. The family members of the PFDM patient with mutation of exon 5 were analyzed by PCR followed by SSCP, and two of them revealed the same mutation. The abnormal band on the SSCP analysis of exon 7 was identified as the insertion of base C/T at the 39th nucleotide in intron 7. Two family members of this patients also had same band on SSCP. The one mutation of 45 normal controls was CT located at the 8th nucleotide in intron 9, which was a common polymorphism. Conclusion: We found GCK mutations in subjects with PTDM and we speculate that these mutations may be one of the contributing cause of PTDM.

만성두통 환자의 성격유형 A 행태, 스트레스, 우울 및 두통영향정도의 관계 연구

차남현,임사비나,정인태,김수영,안경애,김건식,이재동,이상훈,최도영,이윤호,이두익 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

To examine an estimate factor and grasp the relation of difference for Ype A Behavior Pattern(TABP), Perceived Stress Questionnaire. Depression and HIT-6 in the Chronic headache client. Method: Data collected by self-reported questionnaires from 38 client in S city who were selected by criteria of IHS, from the 19^(th) of October to 10^(th) of December, 2004. Result: 1) Differences between biographical data by TABP was significant by SaSang constitutions, by Stress was significantly influenced by age, and by Depression were significantly influenced health status and SaSang constitutions. 2) Correlations Coefficients among Study Variables were Stress and Depression(r=.494, p=.002) and Depression and HIT-6(r=.432, p=.010). 3) In regression analysis, HIT-6 were significantly influenced by Depression and Type A Behavior Pattern(TABP). These variables explained 38% and 34% respectively. Conclusion: The result suggest that chronic headache management with psychological aspect, as well as physical aspect should be a focus to enhance the quality of life.

오로토산 유발 지방산의 간장 트리아실글리세롤 축적간 지방산 조성의 변화

차재영,김경숙,조영수 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.5

본 연구는 오르트산 투여가 흰쥐의 지방간 유발 형성과정에서 간장 및 혈청의 각 지질과 간세포의 지방산 조성에 대하여 경시적으로 함량 변화를 분석하였다. 그 결과 오로트산 투여 흰쥐의 간장 지질의 구성 지방산 중에서 리놀레산 (18:2, n-6)이 모든 각 지방질 중 가장 많이 증가하였다. 리놀레산은 간세포의 소포체 및 골지체의 트리아실글리세롤에서도 증가하였다. 이 지방산 패턴을 경시적으로 분석한 결과, 간장 트리아실글리세롤에서는 오로트산 투여 1일째부터 증가하고, 디아실글리세롤에서도 같은 결과를 나타냈다. 그러나 혈청 트리아실글리세롤에서는 오로트산 투여 1일째에서는 증가하였으나, 2일째부터는 증가율이 둔화되었고, 10일째에서는 대조군보다 저하하였다. 한편, 지방산 중에서 올레인산(18:1, n-9)은 간장 콜레스테릴 에스테르에서만 현저히 상승하였으나, 다른 것에서는 차이가 없었다. 간장 트리아실글리세롤, 디아실글리세롤, 콜레스테릴 에스테릴의 구성 지방산 중에서는 14:0, 16:0, 18:0의 포화지방산은 오르트산 투여로 저하하였고, 역으로 혈청 트리아실글리세롤에서는 이런 지방산이 증가하였다. 이러한 결과로부터, 오르트산 유발 지방간에 있어서 간장 트리아실글리세롤이 축적될 때, 간장지질의 구성 지방산 중에서 18:2의 비율이 현저하게 증가한바, 지방산의 대사 저해와, 간장에서 혈청으로 이행하는 경로가 저해되고 있는 것으로 나타났다. Dietary orotic acid is known to induce the fatty liver. Fatty acid profiles in the lipid fraction of the liver and the serum in rats fed with or without orotic acid diet were analyzed. In all the hepatic lipid fractions of rats fed on the supplemented orotic acid diet, there was a significant increase in linoleic acid. In addition, linoleic acid was also increased in the triacylglycerol faction of hepatic endoplasmic reticulum and the triacylglycerol and diacylglycerol fractions of hepatic Golgi apparatus of the orotic acid-feeding rats. In the time course study of the fatty acid profile in the hepatic triacylglycerol and diacylglycerol fractions, an increase of linoleic acid was observed similarly in the initial stages of orotic acid intake in the both fractions. However, linoleic acid in the serum triacylglycerol fraction of orotic acid-feeding rats increased from day 1, but it began to decrease the increment from day 2, resulting in the lower level of linoleic acid in the serum triacylglycerol fraction of orotic acid-feeding rats than that of rat fed a orotic acid-free diet after 10 days. Oleic acid (18:1) was increased in the only cholesteryl ester fraction of hepatic. However, oleic acid level in other fractions was not changed. The compositions of 14:0, 16:0 and 18:0 was reduced in the hepatic triacylglycerol, diacylglycerol and cholesteryl ester fractions by orotic acid-feeding. However, these saturated fatty acids were significantly increased in the serum triacylglycerol fraction. The orotic acid induced changes in linoleic acid level in hepatic triacylglycerol may be explained by the impaired fatty acid metabolism and limited excretion of this fatty acid from liver to serum.

돼지고기육종의 방사선 유래 휘발성 물질 분석

차용준,박지영,김훈,김소정,정연정,소양순,유영재 창원대학교 생활과학연구소 2000 생활과학연구 Vol.4 No.-

Bacillus cereus KCTC 3674 produced at least two kinds of extracellular proteases. Two major bands of the protease activity with molecular weights of approximately 100 and 38 kDa were obtained after gelatin-SDS-PAGE. The production of extracellular proteases occurred during the logarithmic growth phase and was greatest when cultures reached the stationary growth phase. On the other hand, the growth of B. cereus KCTC 3674 and the production of its extracellular proteases were severely inhibited by the addition of protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP). Thus, it was concluded that the growth of B. cereus KCTC 3674 and the production of its extracellular pretenses were strongly affected by a H+ electrochemical potential.