종돈개량 모니터링시스템에 대한 고찰

도창희(Chang-Hee Do),양창범(Chang-Beom Yang),최재관(Jae-Gwan Choi),양보석(Boh-Suk Yang),송형준(Hyung-Jun Song) 충남대학교 농업과학연구소 2015 농업과학연구 Vol.42 No.3

This paper sketches the strategies and designs for monitoring system of swine genetic improvement. The system should reflect every side of pig production. The system leads us to assess the efficiency of pig production and the scope of the system includes not only nucleus, multiplying and commercial herds, but also packing and processing sectors. For more accurate statistics, data for this monitoring system must be collected from all above mentioned areas, but not by random sampling. Futhermore, data analysis results including seedstocks and distribution information of genetic trend should be included in the system. The schema of knowledge database system could be employed in the system. The monitoring system in the final destination would unify the systems derived from various sources and provide any solution in swine industry including pig breeding.

젖소의 장수성과 수익성 관계 연구

도창희(Chang Hee Do),조재성(Jae Sung Cho),조광현(Kwang Hyun Cho),양보석(Boh Suk Yang),윤호백(Ho Baek Yun),이지수(Ji Su Lee) 충남대학교 농업과학연구소 2015 농업과학연구 Vol.42 No.3

Records of 490,767 cows collected from 1990 to 2012 by dairy herd milk test of National Agriculture Cooperative Federation The pedigree of dairy cattle were provided by Korea Animal Improvement Association. The data were used to analyze the longevity of dairy cows with the life traits such as days in milk, number of lactation, productive life, and life span. The data were also used to investigate genetic relationship of these longevity traits with profitability of dairy cows, including heritability and genetic correlation. The profitability was calculated with simulation of milk income and production costs for individual cows. Days in milk among the traits had ?0.287, -0.572 and ?0.536 of genetic correlation with number of lactations, productive life and lifespan, respectively. The heritabilities of life span, number of lactations, productive life, and days in milk were found to be 0.045, 0.047, 0.059 and 0.081, respectively. Genetic correlations of profit with productive life, number of lactations, and days in milk were identified as 0.072, 0.080, 0.098 and 0.101. These results suggested that days in milk was most desirable traits to represent longevity of Holstein dairy cattle. In general, since longevity and profitability were close genetic relationship each other, genetic improvement of longevity is necessary for better profitable cows.

한우에 있어서 유전체 육종가 추정

이승환,김형철,임다정,당창권,조용민,김시동,이학교,이준헌,양보석,오성종,홍성구,장원경,Lee, Seung Hwan,Kim, Heong Cheul,Lim, Dajeong,Dang, Chang Gwan,Cho, Yong Min,Kim, Si Dong,Lee, Hak Kyo,Lee, Jun Heon,Yang, Boh Suk,Oh, Sung Jong,Hong, S Institute of Agricultural Science 2012 Korean Journal of Agricultural Science Vol.39 No.3

Genomic breeding value (GEBV) has recently become available in the beef cattle industry. Genomic selection methods are exceptionally valuable for selecting traits, such as marbling, that are difficult to measure until later in life. One method to utilize information from sparse marker panels is the Bayesian model selection method with RJMCMC. The accuracy of prediction varies between a multiple SNP model with RJMCMC (0.47 to 0.73) and a least squares method (0.11 to 0.41) when using SNP information, while the accuracy of prediction increases in the multiple SNP (0.56 to 0.90) and least square methods (0.21 to 0.63) when including a polygenic effect. In the multiple SNP model with RJMCMC model selection method, the accuracy ($r^2$) of GEBV for marbling predicted based only on SNP effects was 0.47, while the $r^2$ of GEBV predicted by SNP plus polygenic effect was 0.56. The accuracies of GEBV predicted using only SNP information were 0.62, 0.68 and 0.73 for CWT, EMA and BF, respectively. However, when polygenic effects were included, the accuracies of GEBV were increased to 0.89, 0.90 and 0.89 for CWT, EMA and BF, respectively. Our data demonstrate that SNP information alone is missing genetic variation information that contributes to phenotypes for carcass traits, and that polygenic effects compensate genetic variation that whole genome SNP data do not explain. Overall, the multiple SNP model with the RJMCMC model selection method provides a better prediction of GEBV than does the least squares method (single marker regression).

유전 및 육종 : 한우의 초음파 측정 형질과 도체 형질의 유전모수 추정

김형철 ( Hyeong Cheol Kim ),조영무 ( Young Moo Cho ),이상민 ( Sang Min Lee ),양보석 ( Boh Suk Yang ),김종복 ( Jong Bok Kim ),연성흠 ( Seung Heum Yeon ),이승환 ( Seung Hwan Lee ),당창권 ( Chang Gwon Dang ),전기준 ( Gi Jun Jeon ) 한국동물자원과학회(구 한국축산학회) 2012 한국축산학회지 Vol.54 No.5

본 연구는 한우 번식우와 거세우의 도체형질과 초음파 측정형질 의 유전모수를 추정하여 도체형질과 초음파 측정형질 간의 관계를 알아보기 위하여 실시하였다. 한우 암소에 있어서 초음파 등지방두께의 유전력은 0.43이었고, 초음파 등심단면적의 유전력은 0.23 그리고 초음파 근내지방도는 0.32이었다. 한편, 한우 암소 도체형질의 유전력은 등지방두께는 0.33, 등심단면적이 0.13 그리고 근내지방도는 0.33으로 분석되었 다. 한우 암소에 있어서 초음파 측정 형질 간 유전상관은 초음파 등지방두께: 초음파 등심단면적에서 -0.19, 초음파 등지방두께: 초 음파 근내지방도는 -0.61이었고, 초음파 등심단면적: 초음파 근내 지방도는 -0.36으로 각 형질 간 부의 유전상관을 보였다. 표현형 상관에 있어서는 초음파 등지방두께: 초음파 등심단면적에서 0.03, 초음파 등지방두께: 초음파 근내지방도는 0.13이었고, 초음파 등심 단면적: 초음파 근내지방도는 0.26으로 분석되었다. 한우 암소에 있어서 초음파 측정 형질과 실제 도체형질 간 유전 상관은 등지방두께 0.97, 등심단면적 0.94 그리고 근내지방도 0.92 로 매우 높은 정 (+)의 상관을 나타내었고, 초음파 측정형질과 실제 도체형질 간 표현형상관에 있어서도 등지방두께 0.90, 등심단면적 0.86 그리고 근내지방도 0.81로 높은 정 (+)의 상관계수가 추정되 었다. 그리고 암소에서 나타나는 이러한 결과는 거세우 자료에서나 암소와 거세우를 통합한 자료에서도 일관되게 나타나고 있다. 본 연구에서 초음파 측정치와 실제 도체에서 측정한 값 들 사이 에 유전적으로나 표현형적으로 높은 수준의 정 (+) 상관이 존재하 는 본 연구 결과는 육질 개량을 위한 간접 선발의 도구로 초음파 측정 자료를 활용하는 것이 가능할 것이며 한우의 도축 결과를 상 당히 정확한 수준으로 예측하는 것이 가능하므로 사양관리 측면에 Kim et al. ; Genetic Parameters for Ultrasound and Carcass Traits in Hanwoo -3 3 6- 서도 큰 활용 가치가 있을 것으로 판단한다. This study was conducted to estimate genetic parameters for ultrasound and carcass traits in Hanwoo. Heritabilities and genetic and phenotypic correlations were estimated for carcass and ultrasound measurements collected from Hanwoo cows (n=312) born at Hanwoo experiment station. Traits evaluated were eye muscle area (EMA), backfat thickness (BF), marbling score (MS) from carcass, and ultrasound eye muscle area (UEMA), ultrasound backfat (UBF), and ultrasound marbling score (UMS). Parameters were estimated using multi-trait animal models byderivative-free restricted maximum likelihood procedures. Estimated heritabilities for UBF, UEMA and UMS were 0.43, 0.23 and 0.32, while heritabilities for BF, EMA and MS were 0.33, 0.13 and 0.33 in fattened cows, respectively. Genetic correlations between ultrasound and carcass measurements were estimated to -0.19, -0.61, and -0.36 for UBF: UEMA, UBF: UMS, and UEMA: UMS in fattened cows, respectively. Phenotypic correlations between ultrasound and carcass measurements were 0.03, 0.13 and 0.26 for UBF: UEMA, UBF: UMS, and UEMA: UMS in fattened cows, respectively. As for the steer, genetic correlations between ultrasound and carcass measurements were 0.36, -0.80 and 0.27 for UBF: UEMA, UBF: UMS, and UEMA: UMS in steers, respectively. Phenotypic correlations between ultrasound and carcass measurements were 0.13, 0.07 and 0.41 for UBF: UEMA, UBF: UMS, and UEMA: UMS in steers, respectively. In conclusion, this finding would be very useful to implement into Hanwoo breeding program.

Effects of Reed (Phragmites communis) on Growth Performance, Carcass Characteristics, and Meat Quality in Hanwoo Steers

Sang Min Lee(이상민),Sun Sik Chang(장선식),Ki Yong Chung(정기영),Hyeong Cheol Kim(김형철),Sun Ho Choi(최순호),Ha Yeon Jeong(정하연),Boh Suk Yang(양보석),Sung Sill Lee(이성실),Young Moo Cho(조영무) 한국생명과학회 2012 생명과학회지 Vol.22 No.10

본 연구는 한우 거세우의 비육시 급여 조사료의 종류가 발육 및 도체특성에 미치는 영향을 구명하고자 수행하였다. 한우 거세우 21두를 3처리(건초, 갈대 및 갈대+볏짚), 처리당 7두씩 완전 임의 배치하였다. 시험결과 개시체 중 및 종료체중은 처리구별(대조, T1 및 T2)로 각각 125.5, 128.3 및 128.3 kg과 697.4, 614.6 및 706.7 kg으로 나타났다. 일당증체량은 대조구(0.70 kg/d)와 T2 처리구(0.71 kg/d)가 T1 처리구(0.60 kg/d)보다 향상된 경향을 나타났지만 유의적인 차이는 없었다. 건물섭취량은 유의적인 차이는 없었지만 T1 처리구가 다른 처리구에 비해 비교적 낮았다. 육량특성에서 도체중은 대조구와 T2처리구간에는 차이가 없었지만, T2처리구는T1 처리구에 비해 높았다(p<0.05). 등지방두께와 등심단면적은 대조구와 T2처리구가 T1처리구 보다 높았고, 육량등급(A:B:C, %)은 T1 처리구(43:57:0)가 다른 처리구(대조구 0:71:29, T2 29:42:29)에 비해 높았다. 육질특성에서는 지방색과 조직감은 처리구 간 유의적인 차이가 없었다. 그러나 육색과 성숙도는 T2 처리구에 비해 T1 처리구가 유의적으로 높았다 (p<0.05). 근내지방도 및 육질1등급이상 출현율은 T1처리구에 비해 대조구와 T2처리구가 높았다. 본 연구의 결과를 종합해 볼 때 거세한우 비육시 조사료로서 갈대와 볏짚을 혼합급여할 때 발육, 사료 이용성 및 도체 성적이 향상되어 갈대가 한우의 조사료원으로서 활용가치가 있는 것으로 판단되었다. This study investigated the effects of diet of different forages on growth performance and carcass characteristics of Hanwoo steers. Twenty-one Hanwoo steers were randomly allocated to three groups (fed hay, reed, and reed with rice straw) of seven steers each. Initial and final body weights of control, T1, and T2 groups were 125.5, 128.3, 128.3 kg and 697.4, 614.6, 706.7 kg, respectively. Average daily gain tended to increase in controls (0.70 kg/d) and the T2 group (0.71 kg/d) but not as much in the T1 group (0.60 kg/d); however, there was no significant difference. DMI was not significantly different among the treatment groups, but T1 was relatively lower than the other groups. For the yield traits, carcass weight was not significantly different between controls and the T2 group but was greater in the T2 group compared to the T1 group (p<0.05). Back fat thickness and rib eye area were higher in controls and T2 compared to T1; yield grade (A:B:C, %) was greater in T1 (43:57:0) compared to the other groups (control 0:71:29; T2 29:42:29). For the quality traits, fat color and texture were not significantly different among groups. However, meat color and maturity were significantly greater in T1 compared to T2 (p<0.05). Marbling score and appearance rate of over 1st meat quality grade were greater in the control and T2 groups compared to the T1 group. Based on the results, growth performance, feed utilization, and carcass traits appeared to improve when roughage containing rice straw plus reed was offered. Therefore, reed is worth considering as a roughage source for fattening Hanwoo steers.

반추기축영양 : 한우 암소 비육시 배합사료 급여형태가 발육, 혈액성상 및 도체특성에 미치는 영향

이상민 ( Sang Min Lee ),장선식 ( Sun Sik Chang ),김형철 ( Hyeong Cheol Kim ),최순호 ( Sun Ho Kim ),권응기 ( Eung Gi Kwon ),박병기 ( Byung Ki Park ),양보석 ( Boh Suk Yang ),이성실 ( Sung Sill Lee ),조영무 ( Young Moo Cho ) 한국동물자원과학회(구 한국축산학회) 2013 한국축산학회지 Vol.55 No.1

본 연구는 한우 암소 비육시 배합사료 급여형태가 발육, 혈액성상 및 도체특성에 미치는 영향을 구명하고자 수행하였다. 처리구는 배합사료 제한급여 8개월(T1), 제한급여 6개월 후 자유채식 2개월(T2) 및 제한 4개월 후 자유 4개월급여(T3)로 3처리 9반복으로 완전임의배치 하였다. 배합사료 급여형태별 발육은 처리구간 유의적인 차이가 없었다. 그러나, 일당증체량은 T2 처리구(0.75kg/d)가 다른 처리구(T1: 0.62kg/d, T3: 0.72kg/d)에 비해 높은 경향을 나타내었다. 건물섭취량은 처리구간 유의적인 차이는 없었지만 T1 처리구의 볏짚 섭취량이 다른 처리구 보다 유의적으로 높았다(p<0.05). 사료요구량은 T1: 17.8, T2: 12.8 및 T3: 14.1 kg으로 처리구간 유의적인 차이가 인정되었다(p<0.05). 혈청 내 albumin, triglyceride, glucose 및 GPT의 농도는 비육개시 6개월에서 8개월에 T3 처리구가 다른 처리구에 비해 유의적으로 높게 나타났다(p<0.05). 육량특성에서 도체중, 등지방두께 및 등심단면적은 처리구간 유사한 결과를 보였는데 육량지수는 T2 처리구가 T1 처리구에 비해 유의적으로 높게 나타났다(p<0.05). 육량 A등급 출현율은 T2 처리구가 78%로 모든 처리구에서 가장 높았다. 육질특성 중 근내지방도, 육색, 지방색, 조직감 및 성숙도는 처리구간에 유의적인 차이가 나타나지 않았다. 하지만, 근내지방도와 육질 1등급 이상출현율은 T3 처리구(4.0, 56%)가 다른 처리구(T1: 3.4, 56%; T2: 3.6, 33%)에 비해 높은 경향을 나타내었다. 따라서 본 연구는 한우 암소 비육시 배합사료의 급여는 비육초기 4개월은 제한급여(체중의 1.6%) 하고 비육후기 4개월에 자유채식하는 것이 적절한 것으로 판단되었다. This study was conducted to investigate the effects of feeding patterns of concentrates on growth performance, blood parameters and carcass characteristics in Hanwoo cows. Randomly-allocated groups, restricted concentrate feeding(T1), restricted concentrate feeding for 6 months and ad libitum 2 months(T2), restricted concentrate feeding for 4 months and ad libitum 4 months(T3), were contained with 9 animals. According to feeding patterns of concentrate, growth performance was not significantly different among the treatment groups. However, ADG tended to be higher in T2 group(0.75kg/d) compared to the other groups (T1: 0.62 kg/d, T3: 0.72kg/d). DMI was not significantly different among the treatment groups, interestingly, rice straw intake was significantly higher in T1 group compared with others(p<0.05). There were significant difference among feed conversion ratio, which are 17.8, 12.8, and 14.1 kg for T1, T2, and T3(p<0.05), respectively. The serum level of albumin, triglyceride, glucose and GPT were greater in T3 group compared to other groups at fattening 6 to 8 months(p<0.05). The results of yield traits, carcass weight, back fat thickness and rib eye area were not differ among treatment groups, but yield index was significantly greater in T2 group compared to T1 group(p<0.05). The ``A`` appearance rate(%) of meat yield grade was highest in T2 group for 78%. The marbling score, meat color, fat color, texture and maturity in quality traits were not differ among the treatment groups. However, marbling score and appearance rate(%) of over 1st meat quality grade were tend to be increased at T3 rather (4.0, 56%) group compared with other groups(T1: 3.4, 56%; T2: 3.6, 33%). Thus, the present study suggested that restricted concentrate feeding(1.6% of BW) for 4 months during early fattening periods and ad libitum feeding for 4 months during late fattening periods are recommendable.

Prediction of genomic breeding values of carcass traits using whole genome SNP data in Hanwoo (Korean cattle)

Seung Hwan Lee(이승환),Heong Cheul Kim(김형철),Dajeong Lim(임다정),Chang Gwan Dang(당창권),Yong Min Cho(조용민),Si Dong Kim(김시동),Hak Kyo Lee(이학교),Jun Heon Lee(이준헌),Boh Suk Yang(양보석),Sung Jong Oh(오성종),Seong Koo Hong( 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.3

Genomic breeding value (GEBV) has recently become available in the beef cattle industry. Genomic selection methods are exceptionally valuable for selecting traits, such as marbling, that are difficult to measure until later in life. One method to utilize information from sparse marker panels is the Bayesian model selection method with RJMCMC. The accuracy of prediction varies between a multiple SNP model with RJMCMC (0.47 to 0.73) and a least squares method (0.11 to 0.41) when using SNP information, while the accuracy of prediction increases in the multiple SNP (0.56 to 0.90) and least square methods (0.21 to 0.63) when including a polygenic effect. In the multiple SNP model with RJMCMC model selection method, the accuracy (r²) of GEBV for marbling predicted based only on SNP effects was 0.47, while the r² of GEBV predicted by SNP plus polygenic effect was 0.56. The accuracies of GEBV predicted using only SNP information were 0.62, 0.68 and 0.73 for CWT, EMA and BF, respectively. However, when polygenic effects were included, the accuracies of GEBV were increased to 0.89, 0.90 and 0.89 for CWT, EMA and BF, respectively. Our data demonstrate that SNP information alone is missing genetic variation information that contributes to phenotypes for carcass traits, and that polygenic effects compensate genetic variation that whole genome SNP data do not explain. Overall, the multiple SNP model with the RJMCMC model selection method provides a better prediction of GEBV than does the least squares method (single marker regression).

한우, 칡소 및 제주 흑우 Calpain-Calpastatin 유전자 다양성

이승환,김승창,조수현,최봉환,Aditi Sharma,임다정,당창권,장선식,김재환,고문석,양보석,강희설 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The aim of study was to investigate genetic diversity for the calpain/calpastatin gene in three Hanwoo breeds [(Brown (n=62), Brindle (n=81) and Jeju Black (n=30)]. Random samples from three breeds of Hanwoo were selected and genotyped for the 7 SNPs of calpain/calpastatin using TaqMan method. Allele frequencies were investigated for CAPN1/CAST gene. Allele frequency of CAST2 SNP was 0.75, 0.59 and 0.22 for Brown, Brindle and Jeju black, respectively. The CAST3 revealed allele frequency of 0.59 and 0.57 in Brown and Jeju Black, while it showed very low allele frequency (0.07) in Brindle. In particular, favorable allele (G allele) for the CAPN1-2 SNP which was shown a strong association with tenderness in Taurine and Indicine cattle revealed 16% and 17% higher allele frequency in Brown Hanwoo (0.82) comparing Brindle (0.66) and Jeju Black Hanwoo (0.65). AMOVA demonstrated that among population variance occupied only 10% of total variance and among individual variance was 0%, while within individual variance was 90% of total variance. This result showed that population effect contributed very small portion of genetic to these three Hanwoo breeds, while within individual variance contributed large portion of genetic diversity within these Hanwoo breeds. In conclusion, three Hanwoo breeds (Brown, Brindle and Jeju black) showed a genetically homogeneous based on the 7 SNPs of CAPN1/CAST gene and it came from same ancestor to form modern Hanwoo breed.