쌀의 세척 및 취반에 따른 잔류농약의 제거

황래홍,김애경,정보경,이재규,신재민,박영혜,박혜원,김민정,박경애,윤은선,김무상 한국식품위생안전성학회 2013 한국식품위생안전성학회지 Vol.28 No.1

In this study, the pesticide residues of polished rice and brown rice were measured after washing and cooking process. The initial concentrations of isoprothiolane and fthalide were 7.134 ± 0.03 mg/kg and 2.024 ± 0.04 mg/kg for polished rice, and 17.941 ± 1.41 mg/kg and 6.413 ± 0.19 mg/kg for brown rice, respectively. After first washing process, the removal rates of isoprothiolane were 23.0% (polished rice) and 18.5% (brown rice). Also, the case of fthalide, the removal rates were 14.0% and 9.7%, respectively. The removal dose was increased, but the removal rate was decreased in proportion to the number of washing process, After the cooking process, the removal rates of isoprothiolane and fthalide were 66.0% and 79.0% (polished rice), and 88.7% and 92.9% (brown rice), respectively. The removal rate of isoprothiolane was higher in the washing process, and the case of fthalide was higher in the cooking process. The optimum washing process for the pesticides removal was five times and the cooking process was the most effective to use the new water after soaking the rice in water during 30 minutes. 본 연구에서는 쌀에 인위적으로 조성된 잔류농약이 세척 및 취반 과정에서 제거되는 정도를 측정하였다. 백미의 농약처리 직후 isoprothiolane 과 fthalide의 농도가 각각 7.134 ± 0.03 mg/kg 및 2.024 ± 0.04 mg/kg 이었으며 현미에서는 각각 17.941 ± 1.41 mg/kg 및 6.413 ± 0.19 mg/kg이었다. 1회 세척 후 백미와 현미에서 isoprothiolane의 제거율은 각각 23.0% 및 18.5% 이었으며, fthalide의 제거율은 각각 14.0% 및 9.7% 이었다. 세척 횟수가 증가함에 따라 각 잔류농약의 제거량은 많아지나 제거율은 감소하였다. 취반 후 isoprothiolane 과 fthalide의 제거율은 백미의 경우 각각 66.0% 및 79.0% 이었으며 현미의 경우에는 각각88.7% 및 92.9% 이었다. isoprothiolane의 경우 농약제거율이 취반과정보다 세척과정에서 더 높았으며, fthalide의경우에는 취반과정에서 더 높았다. 농약제거를 위한 가장적합한 세척 및 취반 방법은 5회 세척 후 30분간 물에 불리고 새 물로 취반하는 것이 가장 효과적인 것으로 조사되었다.

수입 과일에 살포된 수확 후 처리농약(Post-harvest pesticide)의 경시적 변화

황래홍,조태희,조인순,엄정훈,박영혜,최부철,김현정,김정헌 한국식품위생안전성학회 2010 한국식품위생안전성학회지 Vol.25 No.3

The post-harvest concentration change of four kind of pesticides, captan, chlorpyrifos, methidathion and kresoxim-methyl was investigated with the storage period for the import fruit. Then the post-harvest concentration was set to 1000, 250, 400 and 157 mgkg−¹ for captan, chlorpyrifos, methidathion and kresoxim-methyl respectively. In case of captan, 0.9-12.5 mgkg−¹ in total fruit base and ND-0.23 mgkg−¹ in the sarcocarp were measured after atomization and 0.7-3.2 mgkg−¹ in total fruit base and ND-0.67 mgkg−¹ in the sarcocarp were measured after four week. For chlopyrifos, menthidathion, and kresoxim-methyl 0.4-2.2, 0.7-3.1 and 1.3-2.1 mgkg−¹ in total fruit base and ND-0.32, ND-0.05 and ND-0.16 mgkg−¹ in the sarcocarp were measured after atomization respectively. After four week 0.3-0.9, 0.4-2.0 and 1.3-1.8 mgkg−¹ in total fruit base and ND-0.02, ND-0.05 and ND-0.15 mgkg−¹ in the sarcocarp were investigated for other three pesticides. The concentration decreasing ratio of pesticides was largest for captan,52% and other components were in order of chlopyrifos, menthidathion, and kresoxim-methy, 47, 41, 11% each other.

매실주 숙성 중 매실부위, 알콜농도 및 trans-Resveratrol 이 에틸카바메이트 생성에 미치는 영향

황래홍,김애경,박경애,김지영,황인숙,채영주 한국식품위생안전성학회 2009 한국식품위생안전성학회지 Vol.24 No.3

The effects of part of plum, alcohol content and addition of t-resveratrol on the formation of ethyl carbamate during the fermentation for wine were investigated at a time interval (45 days) for 6 months. The concentration of the ethyl carbamate in plum wine was determined according to KFDA guideline for ethyl carbamate analysis. In the plum wine with 16% or 30% alcohol content, the concentrations of ethyl carbamate were increased with time of fermentation periods. The maximum concentrations of ethyl carbamate in 16% and 30% plum wines after the fermentation for 6 months were 0.071 μg/g and 0.188 μg/g, respectively. When t-resveratrol was added at the level of 10 μg/g in both 16% and 30% plum wine, the concentrations of ethyl carbamate at 6 months were 0.078 and 0.216 μg/ g, respectively. The addition of t-resveratrol at the level of 300 μg/g in both 16% or 30% plum wine, the concentrations of ethyl carbamate at 6 months were 0.078 and 0.169 μg/g, respectively. The ethyl carbamate in the plum wine was not formed during fermentation for 6 month as using the flesh of plum, but 0.588 μg/g of ethyl carbamate was formed as using plum with plum seed. The addition of 300 μg/g of t-resveratrol actually increased the concentration of the ethyl carbamate by 0.088 μg/g as fermented for 6 months using plum with seed. These results suggest that the flesh of plum should be used to reduce the formation of ethylcarbamate for production of plum wine and that the addition of t-resveratrol during fermentation of plum wine can not reduce the concentration of the ethyl carbamate.

LC-MS/MS를 이용한 농산물중 Neonicotinoid 계 농약분석

황래홍,양혜란,이재규,김창규,김민정 한국식품위생안전성학회 2020 한국식품위생안전성학회지 Vol.35 No.4

A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for neonicotinoid pesticide analysis in agricultural products. Four compounds (imidacloprid, clothianidin, acetamiprid, thiacloprid) were extracted with acetonitrile from agricultural products and cleaned up by NH 2 solid-phase extraction procedure, and eluted with 0.1% formic acid in methanol/dichloromethane (5/95, v/v). The limit of detection and quantification were 0.0001-0.0005 mg/kg and 0.001 mg/kg, respectively. The mean recoveries of neonicotinoid pesticide from agricultural products were in the range of 90.7-100.9% and 94.4-99.8%, as spiked at 0.2 mg/kg and 0.02 mg/kg, respectively. This validation satisfied the national criteria for pesticide analytical methods. In summary, The present method is fast, precise and sensitive enough for the Positive List System (PLS), and we conclude that the method is also suitable for neonicotinoid pesticide determination in a wide range of agricultural products.

LC-MS/MS를 이용한 농산물 중 카벤다짐 분석

황래홍,이성득,김정곤,김지영,박소현,김지혜,박정현,한창호,김무상 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.2

A rapid and precise method using LC-MS/MS was developed for carbendazim analysis in agricultural products. This compound was extracted with acetonitrile from agricultural products and cleaned up by solidphase extraction procedure. The limit of detection and quantification were 0.001 mg/kg and 0.004 mg/kg, respectively. The mean recoveries and precision from 4 agricultural products, soybean sprout and mungbean sprout were in the range of 83.3-86.4% and 0.2-3.0% spiked at 1.0 mg/kg and those were in the range of 77.3-90.1% and 1.3-3.8% spiked at 0.02 mg/kg. The present method is faster and more precise compared with the multi-residue method of Korean Food Code. Therefore, we conclude that this method is suitable for carbendazim determination in a wide range of agricultural products.

서울 강서지역 유통 콩나물 및 숙주나물중 카벤다짐 잔류실태 조사 및 위해성 평가

황래홍,박성규 한국식품위생안전성학회 2019 한국식품위생안전성학회지 Vol.34 No.4

This study was conducted to monitor the carbendazim residues in soybean and mungbean sprouts using LC-MS/MS method. Eighty-two samples were collected from markets in western Seoul. No carbendazim was detected in mungbean sprouts, however, it was detected in 6 among 59 soybean sprout samples showing a 10.2% of detection rate. Among these 6 carbendazim- detected soybean sprout samples, five exceeded the maximum residue limit presenting 83.3% of violation rate. The mean concentration in the detected samples was 0.063 mk/kg (0.012 ~ 0.104 mg/kg) and 5 of the 6 detected samples came from bulk sold products. None of the samples came from domestic products; three were from imported products and 3 were from unidentified sources. Risk assessment for the carbendazim from soybean sprout dietary intake was deemed safe and at less than 1 %Acceptable Daily Intake

학술발표(學術發表) 연제(演題) 및 초록(抄錄) 제일부(第一部): 축산물위생(畜産物衛生) 및 생리(生理) : 5. HPLC를 이용(利用)한 축산식품중(畜産食品中) 잔류(殘留) 설폰아미드제의 동시분석법(同時分析法) 연구(硏究)

황래홍,김영수,윤은선,김기근,이규학 한국가축위생학회 1995 학술대회논문집 Vol.1995 No.-

배추김치와 깻잎지의 제조과정에 따른 잔류농약의 제거

황래홍,조인순,김민정,조태희,박영혜,박혜원,박경애,김현정,김무상 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.4

The removal amount of pesticide residue which were remained in baechu (Chinese cabbage) and perilla leaf were measured during the preparation process of kimchi. The amounts of diazinon, procymidone and endosulfan applied to chinese cabbage were 9.18 ± 0.03 mg/kg, 22.27 ± 0.22 mg/kg and 10.46 ± 0.02 mg/kg respectively. When chinese cabbage was brined with 10% salt solution for 12 hours, the removal rates of three pesticides were 22.5%, 25.3% and 0.6% for diazinon, procymidone and endosulfan, respectively. When chinese cabbage was brined and rinsed 3 times with water, the removal rates of three pesticides were 69.9%, 85.6% and 11.2% for diazinon,procymidone and endosulfan, respectively. When kimchi was prepared and fermented for 28 days at 4oC, the removal rates of three pesticides were 79.4%, 94.4% and 21.0% for diazinon, procymidone and endosulfan, respectively. The relative percentages of removal dose of pesticides during brining were 28.4%, 26.9% and 3.2% for diazinon, procymidone and endosulfan, respectively and which were 59.7%, 63.8% and 50.4% during rinsing and which were 11.9%,9.3% and 46.4% during fermentation, respectively. The amounts of diazinon, procymidone and endosulfan applied to perilla leaf were 18.11 ± 0.62 mg/kg, 31.80 ± 0.33 mg/kg and 12.01 ± 0.01 mg/kg, respectively. When perilla leaf was rinsed 3 times with water, the removal rates of three pesticides were 60.5%, 52.0% and 23.7% for diazinon, procymidone and endosulfan, respectively. When perilla leaf was rinsed and brined with 10% salt solution for 14 days, the removal rates of three pesticides were 93.9%, 92.4% and 49.6% for diazinon, procymidone and endosulfan, respectively. The relative percentages of removal dose of pesticides during rinsing were 64.5%, 56.3% and 47.8% for diazinon,procymidone and endosulfan, respectively, and which during brining were 35.5%, 43.7% and 52.2% for diazinon, procymidone and endosulfan, respectively.

HPLC를 이용(利用)한 축산식품중(畜産食品中) 잔류(殘留) 설폰아 미드제의 동시분석법(同時分析法) 연구(硏究)

황래홍 ( Lae Hwong Hwang ),김영수 ( Young Soo Kim ),윤은선 ( Eun Sun Youn ),김기근 ( Ki Keun Kim ),이규학 ( Kyu Hak Lee ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.3

This study was carried out to explore the most sensitive and useful method for simultaneous determination of five sulfa drugs (sulfamethazine, sulfamerazine, sulfamonomethoxine, sulfadimethoxine, sulfaquinoxaline) in livestock productions (pork muscle, bovine muscle, chicken muscle, milk) by HPLC with UV detector and reverse phase column. The results obtained were as follows: 1. For mobile phase acetonitrile-0.01M ammonium acetate (23:77) showed more applicable sensitivity and retention times than acetonitrile-1% acetic acid (23:77). Thus acetonitrile-0.01M ammonium acetate(23:77) selected and applied to the modification test, from which it was found pH 6.75 was the most adequate. 2. Optimal wavelength of UV for SMT(sulfamethazine), SMR(sulfamerazine), SMM(sulfamonomethoxine), SD(sulfadimethoxine), and SQ(sulfaquinoxaline) were 266, 266, 265, 269 and 250nm, respectivery, and that for simultaneous application it was 263nm. 3. The average recovery rate by extractant(chloroform, dichloromethane, chlorform+dichloromethane) in the classic method was not significantly different(p>0.05) but that by chloroform higher than the others. Thus chloroform was found to be adequate as extractant in this classic method. 4. The average recovery rate was 86.5% by the MSPD(matrix solid phase disperse) method, which was significantly higher than that by the classic method(p<0.05). Also the recovery rates by method were significantly different(p<0.05) in accordance with sample and type of drug. The MSPD method was much superior to classic method on clean-up.

LC/MS를 이용한 축산물 중 잔류 마크로라이드계 항생물질 분석법 연구

황래홍 ( Lae Hwong Hwang ),윤은선 ( En Sun Yun ),김연주 ( Yoen Joo Kim ),김동언 ( Dong Eon Kim ),양윤모 ( Yoon Mo Yang ),이정학 ( Jung Hark Lee ),이병동 ( Byung Dong Lee ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3

This study was carried out to confirm analytical method of residual macrolides in livestock products by LC/MS. 1. Macrolides were analyzed by LC/MS on XTerra C18 column with 0.1%TFA(trifluoroacetic acid)-methanol in a gradient mode as mobile phase, and that were identified by positive chemical ionization with selective ion monitoring at 50~1000 mass range. 2. Residual macrolides were extracted from tissue with acetonitrile, and the extract is purified with a Sep-pak C18 cartridge, and elute macrolides with 0.1M methanolic ammonium acetate. 3. The procedure confirms the presence of each macrolide at 50㎍/kg in spiked sample.