A LINE Element from the Olive Flounder (Paralichthys olivaceus) Shows Similarity to the Maui non-LTR Retrotransposon

김무상,임상욱,황지윤,김지연,김옥순,서정수,안상정,김창배,이형호 한국유전학회 2006 Genes & Genomics Vol.28 No.3

The LINE element, designated Dodalim, has been newly characterized in the olive flounder (Paralichthys olivaceus). The 4,960-bp consensus sequence comprises a 265-bp 5′ untranslated region, as well as a 3,974-bp coding region followed by a 721-bp 3′ untranslated region, which contains the 3′ terminal repeat sequence, TTGAA. The coding region possesses two ORFs (Open Reading Frames), separated by a 41-bp region of non-coding DNA. The predicted product of ORF1 (from position 266 to 1,150) is believed to be a nucleic acid binding protein which contains a zinc finger motif. The ORF2 (from position 1,192 to 4,239) encodes a protein composed of 1,015 amino acids and includes an N-terminal endonuclease domain, a conserved reverse transcriptase domain, and a C-terminal domain of unknown function. Sequence comparisons of the predicted translation products of the ORF2 indicate that Dodalim is closely related to the Fugu Maui non-LTR retrotransposon. The results of our phylogenetic analysis suggest that the LINE ORF2 of fish diverged, in ancient times, from the common ancestor with other chordate, such as avians, reptiles, cephalochords and mammals.

A study on the design of triggering pulse generator for the triggered vacuum switch

김무상,손윤규,박웅화,이병준 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.2

DTP와 전자출판에 관하여

김무상 대한전자공학회 1992 전자공학회지 Vol.19 No.7

토끼 눈에서 플라스민과 히알루론산분해효소의 약리적 유리체융해능력 비교

김무상,문상웅,김응석,유승영,곽형우 대한안과학회 2009 대한안과학회지 Vol.50 No.6

Purpose: The aim of the present study was to quantify and compare the vitreolytic effect of plasmin, hyaluronidase, and a combination of the two. Methods: Thirty-six rabbits were randomized into 3 groups: (A) twelve rabbits had an intravitreal injection of plasmin 1 U with hyaluronidase 10 U/0.1 mL into the right eye, (B) twelve rabbits had an injection of plasmin alone (1 U/0.1 mL), and (C) twelve rabbits had an injection of hyaluronidase alone (10 U/0.1 mL). The left eye of each rabbit was used as control, which was injected with 0.1 mL phosphate buffered saline (PBS). The eyes were enucleated 1 hour and 24 hours after injection. The volume of fluid-type vitreous and gel-type vitreous was measured with a micropipette using the melting point as the difference. Statistical analysis was performed and light microscopy was used to assess potential damage to the retinal tissue. Results: The volume of remaining gel-type vitreous was measured as 52.5%, 60.3%, 59.2%, and 76.5% after 1 hour enucleation and as 44.6%, 56.7%, 56.1%, and 74.7%, after 24 hours enucleation in group A, B, C, and control group, respectively. Group A, B, and C showed statistically significant differences against the control group. Group A (plasmin with hyaluronidase) showed less remaining gel-type vitreous volume than a single injection of plasmin or hyaluronidase alone. Conclusions: Intravitreal injection of plasmin with hyaluronidase showed more vitreolytic effect than a single injection of plasmin or hyaluronidase alone. The enzyme may be useful in liquefying the vitreous, and may be a useful biochemical adjunct to vitrectomy. 목적: 플라스민, 히알루론산분해효소, 이들의 혼합물이 유리체를 융해시키는 능력을 알아보고자 한다. 대상과 방법: 36 마리의 토끼를 12마리씩 3군으로 나누고, 우안에 각각 플라스민, 히알루론산분해효소, 이들의 혼합물을 유리체강내 주사하고 좌안에 인산염완충생리식염수를 주사하여 대조안으로 삼았다. 전체 유리체 중 액화되지 않은 부피를 측정하여 대조안 및 각 군의 결과를 비교하였다. 결과: 대조군 36안에서 24시간 지난 후 겔 상태인 유리체 부피는 전체의 74.7±2.4%였다. 플라스민을 주사한 군에서 56.7±2.4%, 히알 루론산분해효소를 주사한 군에서 56.1±2.0%, 이들의 혼합액을 주사한 경우 44.6±2.1%의 겔 상태 유리체가 남았다. 각 군 모두 대조 안에 비해 유의하게 우수한 유리체융해능력이 관찰되었다(p<0.05). 플라스민과 히알루론산분해효소 각 군 간 비교에서 유리체융해능 력은 유의한 차이를 보이지 않았으나 이들의 혼합물을 주입한 군에서 효소를 단독으로 주입한 군보다 유의하게 더 큰 유리체융해가 관찰되었다(p<0.05). 결론: 토끼 눈에서 플라스민과 히알루론산분해효소는 유리체를 액화시키는 능력이 있을 것으로 생각된다.

양안에 발생한 홍채낭종 1예

김무상,진경현,김동희.Moo-Sang Kim. M.D.. Kyung-Hyun Jin. M.D.. Dong-Hee Kim. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.7

Purpose: Iris cyst is a rare condition and there has been no Korean report of bilateral iris cyst. The authors present the first Korean report of a patient with bilateral iris cysts. Methods: A 62-year-old man presented with intermittent visual field disturbance of the left eye when exposed to bright light. The patient underwent visual field examination and slit lamp examination. On slit lamp examination, a dark-brown mass was revealed along the lateral pupillary margin in the left eye. In the right eye, the shape of the iris was convex at the 8 o`clock direction. Ultrasound biomicroscopy, confirmed the presence of a mass. Results: The patient showed no specific abnormality in visual field examination. Ultrasound biomicroscopy showed the objects to be cystic, with a thin-walled homogenous hypoechoic lesion on the posterior surface of the iris in both eyes. There were two cysts in each eye measuring 4.35 mm×1.13 mm and 1.47 mm×1.11 mm in the left eye, and 1.05 mm×1.02 mm and 1.90 mm×1.05 mm in the right eye. Conclusions: Asymptomatic iris cyst does not need to undergo treatment, but careful examination is nevertheless required required at regular intervals in case a complication develops.

유방 절제술 환자의 척추 측만 변화: 다중채널 전산화단층촬영을 이용한 분석

김무상,김혜원,정선관,최시성 대한영상의학회 2011 대한영상의학회지 Vol.65 No.3

Purpose: To evaluate the relationship between the degree of scoliotic curvature and postoperative change of the chest wall among patients who underwent a unilateral mastectomy. Materials and Methods: Subjects were comprised of 56 women who underwent chest CT and a whole spine standing anteroposterior view (WSSAP). Cobb’s angle and each side of the chest wall volume, including the breast, were measured with the WSSAP and a 3-D reconstructed multi-detector computed tomography (MDCT) image. A correlation analysis was performed between the scoliotic curvature and chest wall volume asymmetry. Directional correspondence between development of scoliosis and undergoing a mastectomy was analyzed. Furthermore, a survey on patient shoulder function was performed using a questionnaire and a correlation was performed between the results of the survey and the scoliotic curvature and chest wall volume asymmetry. Results: The findings indicate that Cobb’s angles were 4.4° ± 2.7 (Mean ± SD, range from 0.6 to 11.4). Differences in chest wall volume were 474.64 ± 276.36 cm^3 (Mean ± SD, range from 78 to 1379). No statistical significance was noted between the degree of scoliotic curvature and chest wall volume asymmetry (p > 0.05). A cross-tabulation analysis of the direction between the scoliotic curvature and mastectomy was found to be statistically significant (p < 0.001). Also, there was a significant correlation between shoulder function assessment score and the degree of scoliotic curvature (p = 0.003), while no significant correlation between shoulder function assessment score and chest wall volume asymmetry (p = 0.091) could be found. Conclusion: Scoliotic change had a tendency to be on the opposite side of the mastectomy and had no statistically significant relationship with the volume asymmetry. Thus, 3-D reconstructed MDCT images are helpful in differentiating selective volume differences. 목적: 유방 절제술을 시행한 환자들에서 유방을 포함한 양측 흉벽의 변화와 척추의 측만곡 변화의 관계를 알아보고자 하였다. 대상과 방법: 대상은 유방암으로 일측유방절제술을 받은 후 흉부 CT로 추적관찰을 시행하던 환자 중, 전척추기립전후촬영을 시행한 56명의 여성 환자였다. 척추 측만곡의 정도를 평가하기 위해 Cobb’s angle을 측정하고 양측의 유방을 포함한 흉벽 조직의 부피를 3차원 재구성 multi-detector CT (이하 MDCT) 영상을 이용하여 측정하였다. 측만 변화와 양측 흉벽 부피의 차이 간의 상관분석을 하였고, 수술 방향에 따른 측만곡 진행 방향에 대한 교차분석을 시행하였다. 또한 어깨 관절 기능 평가를 위한 설문조사를 하여, 측만 변화와 양측 흉벽 부피 차이와의 상관분석을 각각 시행하였다. 결과: 56명의 환자에서 측만 변화는 평균 4.4°± 2.7(범위: 0.6~11.4)이었고, 유방을 포함한 양측 흉벽 부피의 차이는 평균 474.64 cm^3 ± 276.36(범위: 78~1,379)이었다. 측만 변화와 유방을 포함한 양측 흉벽 부피의 비대칭 정도 간의 상관분석에서는 통계학적인 유의성이 없었으나(p > 0.05), 수술 방향에 따른 측만곡은 서로 반대방향으로 나타나는 경향을 보이며 통계적으로 유의한 차이가 있었다(p < 0.001). 어깨 관절 기능 평가 점수는 측만 변화와 유의한 상관관계가 있었으나(p = 0.003), 양측 흉벽 부피 차이와는 유의한 상관관계가 없었다(p = 0.091). 결론: 일측 유방절제술 후 측만 변화는 수술 부위의 반대측에 나타나는 경향을 보였고, 좌우 유방 및 흉벽 부피의 차이와는 유의한 상관관계를 보이지 않았다. 또한, 흉벽 부피 측정에 사용된 3차원 재구성 MDCT 영상은 선택적인 조직의 부피 측정에 유용하다.

자동차 개발에서의 유체공학 응용

김무상 대한기계학회 2006 기계저널 Vol.46 No.9

다리뇌출혈로 인해 발생한 양방향 수평주시마비 1예

김무상,박인기.Moo-Sang Kim. M.D.. In-Ki Park. M.D. 대한안과학회 2005 대한안과학회지 Vol.46 No.10

Purpose: To report a case of bilateral horizontal gaze palsy associated with pontine hemorrhage. Methods: A 51-year-old man developed a sudden decrease in consciousness. Computed tomography reveal a pontine hemorrhage and infarctions of both basal ganglia. The mentality was recovered after conservative treatment, but he complained of difficulty in moving his eyes. We performed ophthalmic and radiologic evaluations to confirm a diagnosis of bilateral horizontal gaze palsy and to reveal the condition. Results: On ophthalmic examination, his eye showed complete bilateral horizontal gaze palsy, but convergence and vertical eye movements were normal. Radiologic evaluation revealed a pontine hemorrhage, and the hemorrhagic area was consistent the bilateral paramedian pontine reticular formation (PPRF).

실차풍동을 이용한 공력 및 공력소음 연구

김무상 대한기계학회 2000 대한기계학회 춘추학술대회 강연집 Vol.- No.-

하지 심부정맥혈전증 환자에서 시행한 동맥을 통한 혈전용해술: 증례 보고

김무상,노병석 대한영상의학회 2011 대한영상의학회지 Vol.65 No.3

If the appropriate catheterization of the affected vein was not possible because of a narrowed or thrombus-filled venous lumen, successful treatment gets into trouble during catheter directed regional thrombolysis for treatment of deep vein thrombosis. In this situation, intra-arterial thrombolysis can be considered as an alternative treatment, but to the best of our knowledge, only two reports have been described. We present here cases of successful intra-arterial thrombolysis in patients with deep vein thrombosis. 하지 심부정맥혈전증 치료를 시행할 때, 이환된 정맥의 직경이 가늘거나 내부가 단단한 혈전으로 가득 차 있어 정맥 내강으로 적절한 도관 삽입이 불가능한 경우, 국소카테터 혈전용해술을 시행할 수 없다. 이러한 경우 동맥을 통한 혈전용해제 투여를 고려할 수 있으나 이에 대한 보고는 2예에 불과하다. 이에 본 저자들은 심부정맥혈전증 치료로 동맥을 통한 혈전용해술을 성공적으로 시행한 증례들을 보고하고자 한다.