섬유아세포에서 민들레 추출물 함유제(AF-343)에 의한 제 1형 교원질 발현 증가

조수묵(Soo Muk Cho),김재현(Jae Hyun Kim),김종근(Jong Keun Kim),박기문(Ki Moon Park),조호찬(Cho Ho-Chan),김인수(In Su Kim),김범준(Beom Joon Kim),천영진(Young-Jin Chun),조재위(Cho Jae We) 대한약학회 2012 약학회지 Vol.56 No.1

We previously reported that the extract of Taraxacum platycarpum (AF-343) had several biological properties such as skin hydration and anti-inflammatory effects, thereby AF-343 be a promising anti-atopic dermatitis agent. However, few studies have been conducted to evaluate its effect on modulation of extracellular matrix proteins in human skin fibroblasts. The purpose of this study was to investigate the expressions of type I collagen, MMP-1, Smad2/3, and TIMP-1 proteins in AF-343-treated human skin fibroblasts. Human skin fibroblasts were treated by various concentrations of AF-343 (0~2 mg/ml). The expressions of type I collagen, matrix metalloproteinase-1 (MMP-1), Smad2/3, and TIMP-1 proteins were analyzed by Western blot analysis. In addition, level of type I collagen mRNA was analyzed by CAT assay. Expression of type I collagen protein was increased in AF-343-treated human skin fibroblasts by dose and time-dependent manners. Consistent with this result, the expressions of phospho-Smad2/3 in skin fibroblasts were increased and MMP-1 expression was decreased by AF-343 treatment. TIMP-1 expression was not significantly changed in AF-343 treated skin fibroblasts. Extract of Taraxacum platycarpum (AF-343)-induced up-regulation of type I collagen expression was through increased expression of phospho-Smad2/3. These results were occurred combined with down-regulation of MMP-1 in skin fibroblasts. Taken together, this study indicated that AF-343 has property of the modulation of ECM in tissue as well as skin hydration and anti-inflammation.

심적환(心適丸)이 산화적 손상에 따른 신경교세포 보호효과 및 국소 뇌혈류량 변화에 미치는 영향

권태우 ( Tae Woo Kwon ),손영수 ( Young Soo Son ),조수인 ( Su In Cho ),김영균 ( Young Kyun Kim ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.4

Objectives: SimJeok-Hwan (CP, Cardiotonic Pills) was made to treat patients with coronary arteriosclerosis, angina pectoris and hyperlipidemia. This study was designed to investigate the effects of CP on Proliferation rates neuroglia cells and protective effect of CP against oxidative stress, and also investigate the effects on regional Cerebral Blood Flow (rCBF) in normal rats. Methods: In this experiment, effects of CP on proliferation rates of neuroglia cells were measured using modified MTT methods. Oxidative stress was induced by treatment with 200 mM of hydrogen peroxide for 2 hr. rCBF and MABP were measured using Laser doppler flowmeter. Results: Treatment with CP elevated proliferation rates in C6 cells. In addition, CP protected cell death of C6 cells induced by oxidative stress. In results, rCBF was elevated by treatment with CP in normal rats. But, Mean Arterial Blood Pressure (MABP) did not affected by CP. In addition, the elevation of rCBF was blacked by pre-treatment with 1 mg/kg of indomethacin effectively. On the other hand, pre-treatment with 0.01 mg/kg of methylene blue did not affect rCBF level in normal rats. Conclusions: In conclusion, these results suggest that CP can act as anti-oxidant to protect neuroglia cells and also suggest that CP can elevate rCBF, which are involved in cyclooxygenase pathway.

채취시기별 보검선인장 줄기의 항산화, 항당뇨 및 항알츠하이머 활성평가

정윤숙 ( Yun Sook Jeong ),황병순 ( Byung Soon Hwang ),조수묵 ( Soo-muk Cho ),황경아 ( Kyung-a Hwang ),황인국 ( In Guk Hwang ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.6

In this study, we assessed antioxidant, anti-diabetic, and anti-Alzheimer activities of Opuntia ficus-indica var. saboten (OFI) at harvest time. OFIs were cultivated December 2015~November 2016 in Jeju island. The 70% ethanol extracts of OFI were used to investigate total polyphenol and flavonoid contents, antioxidant(DPPH and ABTS radical scavenging assay), anti-diabetic(yeast α-glucosidase and rat α-glucosidase inhibition assay), and anti-Alzheimer(Acetylcholinesterase and butyrylcholinesterase inhibition assay) activities. Total polyphenol and flavonoid contents of OFIs were 17.40~23.11 μg garlic acid/mg Ex and 2.17~6.22 ug (+)-catechine/mg Ex, respectively. DPPH and ABTS radical scavenging activity of OFIs were 131.98~184.90 mg ascorbic acid(AA) eq/100 g and 63.60~101.83 mg AA eq/100 g, respectively. In the anti-diabetic and anti-Alzheimer activities, 70% ethanol extracts of OFI exhibited moderate inhibition activity, compared to control (acarbose and beberine). Total polyphenol and flavonoid contents, antioxidant, anti-diabetic, and anti-Alzheimer activities were no significant differences by season, respectively. Therefore, information on comparative biological evaluations of OFI may be a beneficial in exploring functional food and drug development.

문화 차이에 따른 음성 AI 비서의 사용자 경험과 사용자 요구사항 분석

조수인(Soo In Cho),주다영(Da Young Ju) 한국HCI학회 2021 한국HCI학회 학술대회 Vol.2021 No.1

현재 개발된 음성 AI 비서는 사용자의 기대감이 큰 반면, 아직까지 소통적 한계에 부딪혀 사용자의 만족도를 충족하지 못하고 있다. 자연어(Natural Language Understanding) 및 음성 합성 기술의 완성도는 높아졌지만, 복잡한 문장의 응답 등은 아직 어려운 상황이다. 또한 거주 지역, 문화, 사용하는 언어를 고려하지 않고 기업 체에서 일괄적으로 시스템을 개발하였기에 사용자가 겪는 불편함과 UX 의 형태도 다양할 것이라 예상하였다. 이에 본 연구에서는 대한민국, 캐나다, 미국의 거주자 50 명을 대상으로 음성 AI 비서 사용에 대한 사용자 조사를 진행하여 국가, 문화, 언어에 따른 사용자 경험, 불편 사항, 요구하는 니즈(needs)가 분명 하게 다른 점을 파악하여 사용자의 사용성에 대한 차이점을 도출하는 것을 목표로 한다. 이후 연구에서는 사용자의 국가, 문화, 언어의 차이에 따라 세분화한 사용자 경험 연구를 하고자 한다.

재배지역, 수확시기 및 숙기에 따른 여주의 γ-Aminobutyric Acid 함량 변화 및 분석법 검증

이상훈,정윤숙,송진,황경아,조수묵,황인국,Lee, Sang Hoon,Jeong, Yun Sook,Song, Jin,Hwang, Kyung-A,Cho, Soo Muk,Hwang, In Guk 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.3

This study aimed to investigate the changes in the ${\gamma}$-aminobutyric acid (GABA) content of bitter melon (Momordica charantia L.) cultivated from different regions, with different harvest times and at various maturation stages. Methods for observing the changes in GABA content were validated by determining the specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), and precision and accuracy using the HPLC-FLD system. Results showed high linearity in the calibration curve with a coefficient of correlation ($R^2$) of 0.9999. The LOD and LOQ values for GABA were 0.29 and $0.87{\mu}g/mL$, respectively. The relative standard deviations for intra- and inter-day precision of GABA were less than 5%. The recovery rate of GABA was in the range of 98.77% to 100.50%. The average content of GABA was 0.93 mg/g and Cheongju showed highest GABA content of 1.88 mg/g. As the time of harvest increased from May to September, the GABA content decreased from 1.56 to 0.86 mg/g. Also, maturation of the bitter melon fruit was associated with a decreased in GABA content.

북 마크 자동 분류를 위한 학습 에이전트

김인철,조수선,Kim, In-Cheol,Cho, Soo-Sun 한국정보처리학회 2001 정보처리학회논문지B Vol.8 No.5

웹은 이제 인터넷의 중요한 서비스중의 하나가 되었다. 웹 공간을 탐색할 때 사용자들은 항해하는 동한 만나는 흥미 있는 사이트들을 기록하기 위해 북 마크 기능을 이용한다. 북 마크 기능을 이용할때 겪는 문제중의 하나가 거듭된 새로운 북 마크의 추가로 인해 북 마크 리스트의 길이가 길어지면 북 마크 리스트가 일관성 있는 구성을 잃어버리게 되어 실제적인 도움을 주기 어렵다는 것이다. 사용자가 북 마크 파일을 효율적이고 체계적으로 유지하기 위해서는 북 마크 파일에 추가되는 새로운 북 마크들을 카테고리별로 분류하여 신규 폴더를 찾아 삽입해주어야 한다. 본 논문에서는 대응되는 웹 문서들을 다운 받아 내용을 분서함으로써 자동으로 북 마크를 분류하는 BClassifier라 불리는 학습에이전트를 소개한다. BClassifier 에이전트를 위한 훈련 예의 주된 공급원은 바로 사용자가 명시적으로 이미 주제에 따라 몇 개의 북 마크 폴더들로 분류해놓은 북 마크들이다. 여기에 주제 카테고리들을 확대하고 이들에 대한 훈련 문서들을 확보하기 위해 추가적으로 Yahoo 사이트의 최상휘 카테고리들로부터 웹 문서들을 수집하여 훈련 예에 포함시킨다. BClassifier 에이전트는 잘 알여진 확률기반의 분류 기술이나 나이브 베이지안 학습 방법을 채용하고 있다. 본 논문에서는 BClassifier 에이전트에 관한 몇 가지 실험 결과를 소개하고 평가한다. 나이브 베이지안 방법과 k-최근접 이웃 방법, TFIDF 등과 같은 서로 다른 학습 방법들과 비교 실험 결과도 제시한다. The World Wide Web has become one of the major services provided through Internet. When searching the vast web space, users use bookmarking facilities to record the sites of interests encountered during the course of navigation. One of the typical problems arising from bookmarking is that the list of bookmarks lose coherent organization when the the becomes too lengthy, thus ceasing to function as a practical finding aid. In order to maintain the bookmark file in an efficient, organized manner, the user has to classify all the bookmarks newly added to the file, and update the folders. This paper introduces our learning agent called BClassifier that automatically classifies bookmarks by analyzing the contents of the corresponding web documents. The chief source for the training examples are the bookmarks already classified into several bookmark folders according to their subject by the user. Additionally, the web pages found under top categories of Yahoo site are collected and included in the training examples for diversifying the subject categories to be represented, and the training examples for these categories as well. Our agent employs naive Bayesian learning method that is a well-tested, probability-based categorizing technique. In this paper, the outcome of some experimentation is also outlined and evaluated. A comparison of naive Bayesian learning method alongside other learning methods such as k-Nearest Neighbor and TFIDF is also presented.

Designing sustainable personal mobility station based on an advertising business model

Pilbin Kwon(권필빈),Changsu Kim(김창수),Gyeongwon Lee(이경원),Soo In Cho(조수인),Da Young Ju(주다영) 대한인간공학회 2021 대한인간공학회 학술대회논문집 Vol.2021 No.11

Objective: In this study, we propose a station service that guarantees personal mobility (Last Mile Mobility : LMM) users’ sustainable safety and operation of the station by applying an advertising revenue model to the LMM station. The new concept of the station we proposed is called “Lastaion” meaning Last Mile Station. Background: After the COVID-19 pandemic, demand for public transportation is declining and users of LMM have increased. The problems due to LMM continue to occur as the users of LMM increases. From the result of big data analysis in social media, the inconvenience of pedestrians and vehicle drivers due to the chaotic parking of LMM and safety problems caused by LMM users not wearing helmets were emerging as the most essential issues. To solve this problem, major cities around the world are developing LMM stations. In addition, we propose a sustainable LMM station service based on an advertising revenue model to support active usage of future mobility and suggest users use the station continuously. Method: Literature study and big data analysis on trend were conducted to derive current LMM, existing LMM station, and user safety issues. Next, SWOT analysis, SWOT mixed matrix, and STP analysis were performed to propose an advertising revenue model to secure the sustainability of the station. Finally, 3D modeling and 2D rendering were conducted to propose the design of the LMM station, helmet, and application design of the LMM station. Results: Through big data-based analysis, safety problems of LMM, users, and problems of existing LMM stations were derived. First, the LMM parking problem was derived due to the lack of motivation to park in a specific place. Second, due to not wearing helmets brings safety issues to the users. Users avoid wearing helmets because of its uncomfortable fit, weight, volume, and uncleanliness. Third, the problem of existing LMM stations was derived that they could not continue to operate because they did not have their profit model. Fourth, the advertising revenue model is proposed by analyzing various tools. Additionally, the advertising business model of the bus stop, an advertising revenue model that generates profits and enables continuous operation were referenced to structure the business model. Fifth, through the scenario setting and use case analysis, we proposed a station design that is movable compact module suitable for parking lot size. Finally, provide a disposable paper helmet for free of charge by posting advertisements can continue to provide to users a helmet with no charge. Additionally, by providing a station application to users, that will bring users to have a comfortable and convenient user experience. Conclusion: The proposed LMM station service is an advertising-based unique revenue model utilization station. This is the basis for implementing additional services such as providing rewards when returning LMM. Free disposable paper helmets with advertisements encourage users to wear helmets to ensure user safety. Additionally, the user is provided with station information and rescue request service in case of an emergency through the application. Ultimately, Lastation is a sustainable LMM station with serving various functions that secured the safety of mobility user. Application: This study is meaningful in that it can establish a safe future mobility environment and secure user safety by proposing a sustainable LMM station service applying an advertising revenue model.

반하후박탕(半夏厚朴湯)이 생쥐의 심리적 스트레스에 미치는 영향과 유전자 분석

최금애 ( Geum Ae Choi ),조수인 ( Su In Cho ),김경수 ( Kyeong Su Kim ),최창원 ( Chang Won Choi ),위통순 ( Tung Shuen Wei ),양승정 ( Seung Joung Yang ),박수연 ( Soo Yeon Park ),김경옥 ( Kyeong Ok Kim ) 대한한방신경정신과학회 2015 동의신경정신과학회지 Vol.26 No.2

Objectives: Banhahoobak-tang has been used to treat plum-pit qi, chest and hypochondriac distension, moist or greasy tongue coat, and wiry slow or wiry slippery pulse. It might be used to control coughing and vomiting. We observed that Banhahoobak-tang extract (BHTe) had anti-psychological stress effect. The objective of this study was to determine the effect of BHTe on restoring the transcriptional regulation of genes related to psychological stress. Methods: After giving psychological stress to mice, BHTe was orally administered at 100 mg/kg/day for five days. After extracting whole brain tissue from the mice, the gene expression changes were determined by microarray. Transcription factor binding site (TFBS) analysis showed up- and down-regulated genes related to psychological stress were protected by BHTe and segregated according to the structure of TFBS. We performed text based Pubmed search to select significant target genes involved in psychological stress affected by BHTe. Results: 1. Serum corticosterone level was decreased in the BHTe administered group, although the psychological stress was increased. 2. The BHTe administered group had no significant change in noradrenaline content in brain tissue, but the psychological stress group had decreased level. 3. The BHTe administered group had increased time of staying at open-arm than the psychological stress group. 4. Microarray revealed that TANK and RARA genes were up-regulated genes while AES, CDC42, FOS, NCL, and PVR were down-regulated genes by psychological stress but restored by BHTe.

집적 회로에서의 기생 인덕턴스 모델링

민동선(Min Dong-Sun),조수인(Cho Soo-In) 대한전자공학회 1986 대한전자공학회 학술대회 Vol.1986 No.12

홍화(紅花) 추출물 투여에 의한 뇌출혈 흰쥐 뇌조직의 유전자 발현 조절

임세현,손영수,백진웅,조수인,김영균,Lim, Se-Hyun,Son, Young-Soo,Back, Jin-Ung,Cho, Su-In,Kim, Young-Kyun 대한예방한의학회 2008 대한예방한의학회지 Vol.12 No.3

Objectives : The pathophysiology of ICH is not fully understood, therefore, the fundamental therapeutic strategies for ICH also not well inspected either. The genetic profile for the effect of Carthami Flos extract on cerebral hemorrhage in rat brain tissue was measured using microarray technique. Genes displaying expressional change on brain damage were selected and the functional analysis on these genes was conducted. Methods : Rats were placed in a stereotaxic frame after intraperitoneal injection of chloralhydrate, and ICH was induced by injection of collagenase type IV and Carthami Flos extract was administered orally. The molecular profile of cerebral hemorrhage in rat brain tissue was measured using microarray technique to identify up- or down- regulated genes in brain tissue. Results : Upon treatment with Carthami Flos extract on the rat having brain damage, many genes show expressional change. The pattern of gene expressional change can be classified into 8 classes in which two types of classes were composed of recovered genes from up or down-regulation by brain damage, respectively. Conclusions : Further analysis using protein interaction database identified some key molecules that can be used for elucidation of therapeutical mechanism of Carthami Flos extract in future.