생활체육 : 생활체육의 달인

임윤영 한국스포츠정책과학원(구 한국스포츠개발원) 2011 스포츠과학 Vol.117 No.-

복분자뿌리추출물의 지루피부염 치료에의 활용가능성에 관한 연구

임윤영,김범준,장우선,김형미,김민영,오상아,박진오,김연태,박종호,천영진,이지원,문권기,김명남,박영민,강훈 대한의진균학회 2011 대한의진균학회지 Vol.16 No.1

Background: Seborrheic dermatitis is chronic relapsing inflammatory skin disorder. Bokbunja (Rubus coreanus Miquel) is a wild berry to Rosaceae genus and also known to have an anti-inflammation effect. Objective: We were to determine the effect of Rubus coreanus Miquel extract for seborrheic dermatitis in vivo and in vitro. Methods: Seven patients with mild seborrheic dermatitis were enrolled in this study. PCR and culture were performed to identify subtypes of six Malassezia species (M. restricta, M. globosa, M. furfur, M. slooffiae, M. sympodialis, M. obtusa). Topical application of Rubus coreanus Miquel Extract was applied twice daily for 2 weeks. Clinical improvement and safety assessment were performed initially and 2weeks later. Minimum inhibitory concentration (MIC) was evaluated on Malassezia globosa comparing with ketoconazole and itraconazole. Sebum production was also checked prior the experiment and 2weeks later. Results: Five of seven patients showed improvement. No significant adverse effects were found during the clinical trial. Mild dryness was reported in 2 patients but they resolved spontaneously without any treatment. Rubus coreanus Miquel Extract didn't show antimicrobial effect to Malassezia globosa. However, Rubus coreanus Miquel Extract showed anti-inflammatory effect. Conclusion: In this study, we were verified that Rubus coreanus Miquel Extract can be applied for seborrheic dermatitis treatment. And this action mechanism is not related with antimicrobial effect.

StoneTouch(R) 적외선조사기를 이용한 동물 모델에서의 피부 안전성 시험과 아토피피부염의 증상개선 효과에 관한 연구

임윤영 ( Yun Young Lim ),김형미 ( Hyeong Mi Kim ),장우선 ( Woo Sun Jang ),서수홍 ( Soo Hong Seo ),안효현 ( Hyo Hyun Ahn ),김명남 ( Myeung Nam Kim ),김범준 ( Beom Joon Kim ) 대한피부과학회 2011 대한피부과학회지 Vol.49 No.3

Background: Atopic dermatitis is a chronic inflammatory skin disease. It is caused by immunological abnormalities, abnormalities of the skin barrier, environmental factors and genetic factors. Atopic dermatitis destroys the skin barrier and passes through the skin, triggering an immune response. To treat atopic dermatitis, we anticipate use of hypoallergenic cures to hydrate skin that has been dried by destruction of the skin barrier. Objective: We did a preclinical trial to identify inhibitory effects of the StoneTouch(R) infrared scanner on atopic dermatitis. We conducted skin safety tests, comparing the use of infrared energy to drug treatment. We then confirmed the effects of the StoneTouch(R) infrared scanner through animal tests using Nc/Nga mice as a model of atopic dermatitis in order to identify any inhibition of the immune response in atopic dermatitis. Methods: We irradiated Nc/Nga mice using a StoneTouch(R) infrared scanner under a variety of conditions. During skin safety tests of the StoneTouch(R) infrared scanner on hairless mice, we assessed immune response and burn risk in irradiated mouse skin. We identified any inhibitory effects on atopic dermatitis using Dermoscope assessments, measurements of transepidermal water loss (TEWL) and IgE levels, measurements of pro-inflammatory cytokines, H&E staining and immunofluorescence staining (IF) of substance P and CGRP as neurotransmitters on the backs and ears of Nc/Nga mice irradiated by the StoneTouch(R) infrared scanner. Results: We did not observe any skin abnormalities after using the StoneTouch(R) infrared scanner on Nc/Nga mice. We confirmed the inhibitory effect of the StoneTouch(R) infrared scanner irradiation on atopic dermatitis. We found that irradiated epidermis was thinner than that of the epidermis in Nc/Nga mice in which atopic dermatitis was induced. We observed no significant between groups differences in expression level of substance P. The expression of CGRP in mice with atopic dermatitis was decreased, but, the increased irradiation led to greater expression of CGRP in irradiated skin. Conclusion: The StoneTouch(R) infrared scanner does not as a function of irradiation dosage. It inhibits the development of atopic dermatitis. (Korean J Dermatol 2011;49(3):217∼226)

차 부산물로부터 효소를 이용한 캠페롤 생산

임윤영(Yun-Young Lim),김은기(Eun-Ki Kim) 한국생물공학회 2008 KSBB Journal Vol.23 No.2

NC/Nga 마우스에서의 아토피피부염 유사 병변에 대한 Light Emitting Diode의 치료 효과

임윤영 ( Yun Young Lim ),장우선 ( Woo Sun Jang ),김형미 ( Hyeong Mi Kim ),김인수 ( In Su Kim ),이진웅 ( Jin Woong Lee ),김명남 ( Myeung Nam Kim ),김범준 ( Beom Joon Kim ) 대한천식알레르기학회 2011 천식 및 알레르기 Vol.31 No.3

Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease. It is caused by immunological abnormalities, abnormalities of the skin barrier, and environmental/ genetic factors. We did a preclinical trial to identify the effects of the 633-nm light- emitting diode (LED) and 830-nm LED for AD-like lesions in NC/Nga mice. Methods: AD-like skin lesions were induced by topical application of Dermatophagoides farinae extract on the skin of 5-week-old NC/Nga mice for 2 weeks, and then was treated with 630-nm or 830-nm LED for 1 week. We identified any therapeutic effects on AD using modified SCORAD index, skin biopsy, and measurements of both transepidermal water loss (TEWL) and proinflammatory cytokines. Results: Both of 630-nm and 830-nm LED treatment groups showed significantly reduced SCORAD indices and TEWLs at the end of treatment, compared to the non-treatment group. In addition, the levels of proinflammatory cytokine levels in both of the LED treatment groups were significantly decreased compared to those in the non-treatment group. Conclusion: These results show that 633-nm and 830-nm LED treatments can improve -like lesions in NC/Nga mice. (Korean J Asthma Allergy Clin Immunol 2011;31:207-214)

Malassezia 효모균 동정을 위한 Multiplex PCR 기법 개발과 평가

김형미,임윤영,박은주,천영진,김명남,이동훈,김범준 대한의진균학회 2010 대한의진균학회지 Vol.15 No.2

Background: Malassezia yeasts as major pathogenic fungi causes the common skin diseases including dandruff, psoriasis, seborrheic dermatitis and atopic dermatitis etc. various molecular techniques were developed to identify and classify the Malassezia species until now. But, these methods were discovered the problems. So, the development of the better molecular methods required to identify and classify of Malasseiza species. Objective: We sought to develop of molecular techniques to identify and classify of six Malassezia species (M. restricta, M. globosa, M. furfur, M. slooffiae, M. sympodialis, M. obtusa). Methods: We designed primers about ITS1 (Internal transcribed space 1) region that were wellknown region useful to identify of Malassezia species. Because, ITS1 region that is located between 18S and 5.8S rDNA of ribosomal DNA was comparatively mutated quickly. The mono PCR using ITS1 primers was performed to confirm the specificity of ITS1 primers with six Malassezia standard strains. Then, Malassezia Multiplex detection kit was developed on the basis of technique using ITS1regions. Malassezia Multiplex detection kit was used to perform multiplex PCR with six Malassezia standard strains and clinical isolates. Results: The results of mono PCR using ITS1 primers about six Malassezia standard strains was detected each Malassezia standard strains. Also, the multiplex PCR using developed Malassezia Multiplex detection kit was confirmed to classify about six Malassezia standard strains and clinical isolates. Conclusion: In this study, we verified that six Malassezia yeasts was classified using Malassezia Multiplex detection kit from Malasszia standard strains and clinical isolates. And we anticipate that Malassezia Multiplex detection kit is able to do accurate diagnosis about six Malassezia yeasts (M.restricta, M. globosa, M. furfur, M. slooffiae, M. sympodialis, M. obtusa).

Macrophage Raw 264.7 세포와 아토피성 피부염이 유도된 NC/Nga Mice에서 AF-343에 의한 염증 억제 효과와 피부 보습 효과에 관한 연구

김범준,임윤영,김형미,박은주,김명남,최창숙,박기문,김현석,김종근,홍연표,조수묵 대한의진균학회 2010 대한의진균학회지 Vol.15 No.2

Background: Inflammatory response on LPS and IFN-γ induced Macrophage Raw 264.7 cells was secreted NO (nitric oxide) and PGE2 (prostaglandin E2) though expression of iNOS and COX-2. And many pro-inflammatory cytokines (TNF-α, IL-1β, IL-6 etc.) was secreted on LPS and IFN-γ induced Macrophage Raw 264.7 cells, too. Atopy dermatitis was inflammatory skin disease with pruritus,xeroderma and specific eczema. Objective: We sought to effect of anti-inflammation and skin hydration of AF-343 on Macrophage Raw 264.7 cells and NC/Nga mice with Atopic Dermatitis. Methods: The immune response of Raw 264.7 cells were induced by LPS and IFN-γ. Then LPS and IFN-γ induced Raw 264.7 cells was measured NO, PGE2 production after treatment of different concentrations for AF-343. The related genes (iNOS, COX-2) for NO, PGE2 production were detected using Western blot in LPS and IFN-γ induced Raw 264.7 cells after treatment of different concentrations for AF-343. Pro-inflammatory cytokines were detected, too. NC/Nga mice as Atopy dermatitis model was induced atopy dermatitis. Then NC/Nga mice with atopy dermatitis were performed oral administration of AF-343 for 1weeks. After oral administration of AF-343, TEWL was measured on skin tissues of NC/Nga mice with atopy dermatitis according to whether were performed oraladministration of AF-343 or not. And pro-inflammatory cytokines and IgE was measured in serum,protein of skin tissues of NC/Nga mice. Skin tissues of NC/Nga mice were performed H&E staining,immunohistochemical staining for PCNA, Involucrin and filaggrin. Results: LPS and IFN-γ induced Raw 264.7 cells was decreased NO, PGE2 production in dose-dependent after treatment of different concentrations for AF-343. The expression level of iNOS,COX-2 protein was decreased in dose-dependent, too. The related pro-inflammatory cytokines in media with LPS and IFN-γ induced Raw 264.7 cells were decreased after treatment of different concentrations for AF-343. TEWL level of NC/Nga mice skin (back, ear) with atopy dermatitis according to whether were performed oral administration of AF-343 or not was decreased in NC/Nga mice with atopy dermatitis group was performed oral administration by AF-343. When NC/Nga mice group with atopy dermatitis was performed oral administration by AF-343, induced pro-inflammatory cytokines and IgE expression in serum, protein of back, ear skin tissues of each NC/Nga mice group was decreased. H&E stained Skin tissues of NC/Nga mice was confirmed that thickness of epidermis, dermis were decreased in NC/Nga mice group with atopy dermatitis was performed oral administration by AF-343 than NC/Nga mice group with atopy dermatitis. The expression of PCNA, involucrin and filaggrin were decreased in NC/Nga mice group with atopy dermatitis was performed oral administration by AF-343than NC/Nga mice group with atopy dermatitis as results of immunihistochemical staining using specific antibodies such as PCNA as cell proliferation marker, involucrin and filaggrin as keratinocytes differentiation markers for skin tissues (back, ear) of NC/Nga mice. Conclusion: We confirmed effect of anti-inflammation and skin hydration of AF-343 on Macrophage Raw 264.7 cells and NC/Nga mice with Atopic Dermatitis. In conclusion, AF-343 is expecting as therapeutics for atopic dermatitis.

황금추출물의 추출 방법에 따른 세포 독성 평가 및 피부 안전성 평가

김형미 ( Hyeong Mi Kim ),임윤영 ( Yun Young Lim ),조수묵 ( Soo Muk Cho ),김민영 ( Min Young Kim ),손인평 ( In Pyeong Son ),석장미 ( In Pyeong Son ),박진오 ( Jin Oh Park ),박종호 ( Jong Ho Park ),조재위 ( Jae We Cho ),김범준 ( Beom 대한피부과학회 2012 대한피부과학회지 Vol.50 No.11

Background: Scutellaria baicalensis Georgi extract is used as a traditional herbal medicine. The efficacy of Scutellaria baicalensis Georgi extract is known for antioxidative activity, antiinflammation effect, antibacterial effect, inhibitory effect of melanin synthesis, sun protection effect, antiallergy effect, and etc. Objective: We confirmed the cell viability or inhibitory effect of melanin synthesis in HaCaT (human keratinocyte cell line) and B16F10 (murine melanoma cell line) cells and the skin safety test through a clinical test (dermal irritation study) for Scutellaria baicalensis Georgi extract, according to the extraction methods. Methods: We checked the cell viability, using MTT assay and inhibitory effect of melanin synthesis in B16F10 cells or HaCaT cells for thirty one Scutellaria baicalensis Georgi extract, according to the extraction methods. Then, we evaluated the skin safety for selected eight Scutellaria baicalensis Georgi extract through a primary dermal irritation test. Results: Among the thirty one Scutellaria baicalensis Georgi extracts, according to the extraction methods, we selected eight Scutellaria baicalensis Georgi extracts that were not detected with cell toxicity in HaCaT cells and B16F10 cells, and could have inhibited the melanin synthesis in B16F10 cells. The selected eight Scutellaria baicalensis Georgi extracts identified the skin safety through a primary dermal irritation test. Conclusion: We expect clinical trials for whitening efficacy based on inhibitory effect of melanin synthesis and human skin safety for Scutellaria baicalensis Georgi extracts. (Korean J Dermatol 2012;50(11):959∼968)

한국의 아토피피부염 환자에서 혼합생약추출물 함유 로션의 효능에 대한 예비연구

손인평 ( In Pyeong Son ),임윤영 ( Yun Young Lim ),김형미 ( Hyeong Mi Kim ),김민영 ( Min Young Kim ),석장미 ( Jang Mi Suk ),김영희 ( Young Heui Kim ),김기호 ( Ki Ho Kim ),김범준 ( Beom Joon Kim ),김명남 ( Myeung Nam Kim ) 대한천식알레르기학회 2012 천식 및 알레르기 Vol.32 No.1

Background: The choice of topical agents is very important for patients with atopic dermatitis (AD) because mild to moderate AD-related symptoms and skin lesions could be significantly relieved with appropriate topical treatment. This study was performed to evaluate the effectineness of a mixed herbal extract lotion containing the extract of Sophorae Radix, Cinnamomi Cortex, Saururi Herba Seu Rhizoma and Aurantii Immaturus Fructus in relieving symptoms and skin lesions associated with AD. Methods: A total of 24 patients who were suffering mild to moderate AD applied topical lotions, i.e. the test lotion on the right antecubital fossa and the control lotion on the left antecubital fossa twice daily for up to 4 weeks. The treatment efficacy was evaluated by the measurement of skin hydration and trans-epidermal water loss (TEWL) on the same site at each visit. Additional evaluation methods were the investigator global assessment (IGA) and the visual analogue scale visual analogue scale for pruritus. Results: The test lotion-applied right antecubital fossa achieved more clinical improvement than the control lotion-applied left antecubital fossa. TEWL on the test side more significantly decreased than that on the control side after 4 weeks of lotion application. There were slightly more improvements in both the IGA and patient assessment of pruritus on the test side. Conclusion: Mixed herbal extract lotions containing the extracts of Sophorae Radix, Cinnamomi Cortex, Saururi Herba Seu Rhizoma and Aurantii Immaturus Fructus can relieve symptoms, such as pruritus, in AD patients. (Korean J Asthma Allergy Clin Immunol 2012;32:26-33)

치료심포지엄 : 기미에서 plus yellow LaserTM의 치료효과에 대한 연구

이혜인,박주희,박귀영,김연진,임윤영,홍유진,김동석,김범준,김명남,이창균 대한피부과학회 2008 초록집 Vol.46 No.20