Estimating genetic diversity and population structure of 22 chicken breeds in Asia using microsatellite markers

노희종,Kim Seung-Chang,Cho Chang-Yeon,Lee Jinwook,전다연,Kim Dong-kyo,Kim Kwan-Woo,Afrin Fahmida,고응규,이준헌,Batsaikhan Solongo,Susanti Triana,Hegay Sergey,Kongvongxay Siton,Gorkhali Neena Amatya,Thi Lan Anh Ng 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.12

Objective: Estimating the genetic diversity and structures, both within and among chicken breeds, is critical for the identification and conservation of valuable genetic resources. In chickens, microsatellite (MS) marker polymorphisms have previously been widely used to evaluate these distinctions. Our objective was to analyze the genetic diversity and relationships among 22 chicken breeds in Asia based on allelic frequencies. Methods: We used 469 genomic DNA samples from 22 chicken breeds from eight Asian countries (South Korea, KNG, KNB, KNR, KNW, KNY, KNO; Laos, LYO, LCH, LBB, LOU; Indonesia, INK, INS, ING; Vietnam, VTN, VNH; Mongolia, MGN; Kyrgyzstan, KGPS; Nepal, NPS; Sri Lanka, SBC) and three imported breeds (RIR, Rhode Island Red; WLG, White Leghorn; CON, Cornish). Their genetic diversity and phylogenetic relationships were analyzed using 20 MS markers. Results: In total, 193 alleles were observed across all 20 MS markers, and the number of alleles ranged from 3 (MCW0103) to 20 (LEI0192) with a mean of 9.7 overall. The NPS breed had the highest expected heterozygosity (Hexp, 0.718±0.027) and polymorphism information content (PIC, 0.663±0.030). Additionally, the observed heterozygosity (Hobs) was highest in LCH (0.690±0.039), whereas WLG showed the lowest Hexp (0.372±0.055), Hobs (0.384±0.019), and PIC (0.325±0.049). Nei’s DA genetic distance was the closest between VTN and VNH (0.086), and farthest between KNG and MGN (0.503). Principal coordinate analysis showed similar results to the phylogenetic analysis, and three axes explained 56.2% of the variance (axis 1, 19.17%; 2, 18.92%; 3, 18.11%). STRUCTURE analysis revealed that the 22 chicken breeds should be divided into 20 clusters, based on the highest ΔK value (46.92). Conclusion: This study provides a basis for future genetic variation studies and the development of conservation strategies for 22 chicken breeds in Asia.

Microsatellite marker를 이용한 제주 및 4개 관리농가 보유 재래닭의 분자유전학적 특성

노희종,조창연,전다연,김승창,이성수,이진욱,김관우 한국가금학회 2017 한국가금학회 정기총회 및 학술발표회 Vol.2017 No.11

초위성체 마커를 활용한 가축다양성정보시스템(DAD-IS) 등재 재래닭 집단의 유전적 다양성 분석

노희종,김관우,이진욱,전다연,김승창,고응규,문성실,이현정,이준헌,오동엽,변재현,조창연 한국가금학회 2019 韓國家禽學會誌 Vol.46 No.2

A number of Korean native chicken(KNC) populations were registered in FAO (Food and Agriculture Organization) DAD-IS (Domestic Animal Diversity Information Systems, http://www.fao.org/dad-is). But there is a lack of scientific basis to prove that they are unique population of Korea. For this reason, this study was conducted to prove KNC’s uniqueness using 25 Microsatellite markers. A total of 548 chickens from 11 KNC populations (KNG, KNB, KNR, KNW, KNY, KNO, HIC, HYD, HBC, JJC, LTC) and 7 introduced populations (ARA: Araucana, RRC and RRD: Rhode Island Red C and D, LGF and LGK: White Leghorn F and K, COS and COH: Cornish brown and Cornish black) were used. Allele size per locus was decided using GeneMapper Software (v 5.0). A total of 195 alleles were observed and the range was 3 to 14 per locus. The MNA, Hexp, Hobs, PIC value within population were the highest in KNY (4.60, 0.627, 0.648, 0.563 respectively) and the lowest in HYD (1.84, 0.297, 0.286, 0.236 respectively). The results of genetic uniformity analysis suggested 15 cluster (ΔK = 66.22). Excluding JJC, the others were grouped in certain cluster with high genetic uniformity. JJC was not grouped in certain cluster but grouped in cluster 2 (44.3%), cluster 3 (17.7%) and cluster8 (19.1%). As a results of this study, we can secure a scientific basis about KNC’s uniqueness and these results can be use to basic data for the genetic evaluation and management of KNC breeds.

Paraquat 중독 환자에 대한 hemoperfusion 과 continuous veno-venous hemofiltration 의 치료 효과

노희종(Hee Jong Noh),구자룡(Ja Ryong Koo),이정열(Jeong Yeol Lee),주민하(Min Ha Joo),전만조(Man Jo Jeon),김진철(Jin Cheol Kim),김근호(Gheun Ho Kim),전노원(Rho Won Chun),김형직(Hyung Jik Kim),채동완(Dong Wan Chae),노정우(Jung Woo Noh) 대한내과학회 2000 대한내과학회지 Vol.59 No.6

N/A Background : In Korea, paraquat accounts for most of fatal poisoning with 500 or more deaths per year. It has been known that patients who ingested more than 1/2 mouthful of 20% concentrate paraquat usually die of multiorgan failure and pulmonary fibrosis. But the effect of charcoal hemoperfusion which can enhance elimination of paraquat remains controversial. Because acute paraquat poisoning is also characterized by multiorgan failure including kidney and marked rebound in plasma paraquat level after hemoperfusion, Continuous veon-venous hemofiltration(CVVH) may have theoretical benefits in the treatment of paraquat poisoning. So we evaluated the effect of early charcoal hemoperfusion and prophylactic CVVH after hemoperfusion in patients with paraquat poisoning. Methods : There were 80 patients with paraquat poisoning admitted within 24 hours after ingestion (August 1996 - March 1998). All of them were treated with hemoperfusion (duration of hemoperfusion, 6.4±3.0 hours) within 24 hours of ingestion. The amount of ingestion was 2.1±1.0 mouthful (as 20% concentrate) and 78 (98%) were urine sodium dithionite test positive which is a poor prognosis factor. Forty-four patients received hemoperfusion only and 36 were followed by CVVH (duration, 50.4±20.9 hours; ultrafiltration volume, 33.8±3.9 L/day) after hemoperfusion. Results : There was no difference between the hemoperfusion group and hemoperfusion+CVVH group in age, sex, initial serum creatinine, arterial oxygen saturation, severity of poisoning (as assessed by serum paraquat level determined by HPLC and amount of ingestion), or in the time elapsed from ingestion to the beginning of hemoperfusion. The total mortality was 65% (52/80) with no difference between the hemoperfusion group (64%, 28/44) and hemoperfusion+CVVH group (67%, 24/36). The mortality according to amount of ingestion was as follows: 〈 1 mouthful, 0% (0/5); 1 mouthful, 53% (8/15); 2 mouthful, 65% (11/17); ≥ 3 mouthful, 82% (27/33); unknown, 60% (6/10). Conclusion : Early hemoperfusion can be effective in reducing mortality in patients who ingest less than 2 mouthful. Even though prophylactic CVVH after hemoperfusion has no additional benefit in reducing mortality in paraquat poisoning, it prolonged the time to death after ingestion.(Korean J Med 59:651-656, 2000)

MS 마커를 활용한 지역별 오계 유전자원의 다양성 및 유연관계 분석

노희종(Hee-Jong Roh),김관우(Kwan-Woo Kim),이진욱(Jin-Wook Lee),전다연(Da-Yeon Jeon),김승창(Seung-Chang Kim),전익수(Ik-Soo Jeon),고응규(Yeoung-Gyu Ko),이준헌(Jun-Heon Lee),김성희(Sung-Hee Kim),백준종(Jun-Jong Baek),오동엽(Dong-Yep Oh) 한국가금학회 2018 韓國家禽學會誌 Vol.45 No.3

본 연구는 연산오계(천연기념물 제265호)와 이를 기원으로 하는 5개 지역별 오계 집단의 유전적 특성 및 차별성을 분석하기 위해 25개의 초위성체(MS) 마커를 이용하여 총 9개 집단 243수를 대상으로 유전자형을 분석하였다. 마커별 다형성 분석 결과, 총 153개의 대립유전자가 확인되었으며, Hexp와 PIC의 경우 MCW0145에서 각각 0.640, 0.570으로 가장 높았고, Hobs는 MCW0252에서 0.607로 가장 높은 값을 나타내었다. 반면, LEI0166에서 Hexp, Hobs, PIC가 각각 0.248, 0.204, 0.202로 가장 낮았다. 집단간 유전거리 분석 결과로는 9개 집단중 YSO 집단과 SUO 집단이 가장 가까운(0.073) 반면, LG 집단과 CBO 집단 사이에서 가장 먼(0.937) 것으로 확인되었다. 집단의 실제 구조를 확인하기 위한 집단별 균일도를 분석한 결과, 공시된 9개의 집단은 3개의 집단으로 구분했을 때 최적의 K값(7.96)을 얻을 수 있었으며, 5개의 오계 집단(YSO, ARO, CBO, CNO, SUO) 및 LG 집단과 CN․RIR 집단은 각각 1, 2, 3번 군집에 분포하고 있는 것으로 나타났다. 한편, GBO 집단의 경우 1번과 3번 클러스터에 걸쳐서 분포하고 있는 것으로 보아 사육과정에서 타집단과의 교잡이 일어났을 것으로 추정된다. 이러한 결과를 통해 추후 오계 유전자원에 대한 국가 수준의 유전적 특성평가 및 관리의 기초 자료로 유용하게 활용될 것으로 기대된다. The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (ΔK=7.96). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea.

단일염기다형성 마커를 이용한 백우 품종 식별 방법

김승창,김관우,노희종,김동교,김성우,김찬란,이상훈,고응규,조창연 한국산학기술학회 2020 한국산학기술학회논문지 Vol.21 No.1

This study was conducted to develop specific Single Nucleotide Polymorphism (SNP) markers to identify the genetic characteristics and breed of White Hanwoo (WH) using a molecular biological method. SNP genotyping was performed with an Illumina Bovine HD 777K SNP chip using DNA extracted from 48 Hanwoo and 22 WH. The minor allele frequency (MAF) difference of each SNP was calculated and the statistical significance (P-value) of the MAF difference was calculated through Fisher's Exact test (Genotype). SNPs with 100% difference in the MAF difference were selected based on marker selection criteria. The nine SNP markers with genetic differences were selected. The selected markers have different alleles as being Hanwoo- and WH- specific. Therefore, based on these results, it can be concluded that the Hanwoo and WH varieties can be clearly distinguished by using these SNPs. So, the patent of the WH breed identification markers was registered. WH is a breed that shows the characteristics of a Korean native species that is separate from the native Hanwoo. It is expected that genetic characteristics research on the WH can be used to identify the breed and as a knowledge base for enhancing the value of breeding stock. 본 연구는 백우 품종 육성을 위해 분자생물학적 방법을 이용하여 유전적 특성을 파악하고 백우 품종을 식별하기 위한 백우 품종 특이적인 Single Nucleotide Polymorphism (SNP) 마커를 개발하기 위해 수행되었다. 한우 48두와 백우 22두의 혈액에서 추출된 DNA를 이용하여 Illumina Bovine HD 777K SNP chip으로 SNP genotyping을 실시하였다. 각 SNP의 Minor Allele Frequency (MAF) difference (한우와 백우의 차이 절대값)을 계산하고, Fisher’s Exact test (Genotype)을 통해 MAF difference의 통계적 유의성(P-value)을 계산하였다. 품종 별 차이를 나타낼 수 있는 마커를 선발기준으로 MAF difference가 100%의 차이를 나타내는 SNP를 식별하였다. 이러한 유전적 차이를 보이는 9개의 단일염기다형성 마커(rs42125585, rs42125591, rs42125833, rs109461720, rs134735704, rs109447299, rs42164846, rs42160000 및 rs137353829)가 선발되었다. 선발된 마커들은 한우와 백우 특이적인 대립유전자를 가지고 서로 다른 대립유전자를 나타내고 있다. 이들 9개의 SNP 마커들을 이용하여 한우와 백우의 품종을 식별할 수 있음을 확인하였고, 이러한 결과들을 바탕으로 백우 품종 식별 마커 특허를 등록하였다. 백우는 원종인 한우에서 분리되어 한국 재래종의 특성을 잘 나타내 주는 계통으로, 이러한 백우가 가지고 있는 유전적 특성 연구는 백우를 식별하고 품종으로서 육종하는데 사용되어 종축으로서의 가치 증진을 위한 기반 연구가 될 것으로 생각된다.

특별강연 및 일반연제 발표 : Sodium dithionite를 이용한 간편한 혈청 paraquat 농도 측정법의 임상적 의의

구자룡 ( Gu Ja Lyong ),노희종 ( No Hui Jong ),한상진 ( Han Sang Jin ),박규용 ( Park Gyu Yong ),김근호 ( Kim Geun Ho ),전노원 ( Jeon No Won ),김형직 ( Kim Hyeong Jig ),채동완 ( Chae Dong Wan ),노정우 ( No Jeong U ) 대한신장학회 1999 춘계학술대회 초록집 Vol.18 No.2

Zigbee 무선통신 네트워크 기반 변형측정 시스템 설계 및 개발

김상석,박장식,고석조,노희종,Kim, Sang-Seok,Park, Jang-Sik,Go, Seok-Jo,Ro, Hee-Jong 한국전자통신학회 2012 한국전자통신학회 논문지 Vol.7 No.3

본 논문에서는 구조물의 안전한 관리를 위하여 진동현방식 센서를 이용하여 변형을 측정하고 Zigbee 무선 네트워크를 통하여 원격 모니터링할 수 있는 시스템을 구현하였다. 구현한 변형측정기는 진동현방식 센서를 구동하여 출력되는 신호의 주파수를 측정하여 변형을 계산하고, 변형 측정에 있어 요구되는 온도 보상을 위한 온도를 측정한다. 2축 가속도센서를 이용하여 변형의 방향을 측정할 수 있다. 개발한 변형측정기에는 보다 효율적인 모니터링을 위하여 유무선 통신 기능이 있다. 실험을 통하여 개발한 진동현방식 변형측정 시스템이 원격모니터링에 효과적임을 확인한다. In this paper, a system using vibrating wire sensor and zigbee wireless network has been implemented to monitor and manage the structure. The implemented strain controller drives vibrating wire sensor and computes strain from measuring frequency of the output signal. Temperature sensor is included to compensate strain by temperature. Using two axis acceleration sensor of strain controller can measure the direction of strain or deformation. To measure strain more effectively, wired and wireless communication function is included in this device. As results of experiments, it is shown that the developed system can be effectively applied to measure strain of the structure.

56. 방목 사육이 엘크 사슴의 번식능력에 미치는 영향

김관우,이상훈,고응규,전다연,김성우,김승창,노희종,이진욱 한국초지조사료학회 2018 한국초지조사료학회 학술대회논문집 Vol.2018 No.09

엘크 암사슴의 체중 성장곡선 모수 추정

이성수,이진욱,김관우,김상우,이용재,김성우,조창연,노희종,전다연,김동교,연성흠,최태정 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2018 동물자원연구 Vol.29 No.2

The objective of this study was to estimate the growth curve parameters of body weight for female Elk. Weight and age data from 115 does raised at the Animal Genetic Resources Research Center in Korea were used in this study. The growth curve parameters were estimated from a nonlinear regression using Gompertz and Logistic models. Mature weight (), growth ratio () and maturing rate () of female Elk were 214.1±2.17 kg, 2.12±0.0045 and 0.0043±0.00011, respectively, according to the Gompertz model. They were 208.3±2.17 kg, 5.56±0.234 and 0.0065±0.00017, respectively, according to the Logistic model. The goodness of fit determined by R2 was higher in the Gompertz model than that in the Logistic model. The growth curve functions obtained from the Gompertz and Logistic models in female Elk were and , respectively. The absolute growth rate functions from the Gompertz and Logistic models in female Elk were and respectively. The growth pattern of female Elk generated from this study can be useful in determining the most appropriate feeding plans and the best breeding strategies for deer.