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한국 예능프로그램의 중국 현지화 만족도 영향요인에 관한 연구
곽문화,이원희 한국상품학회 2023 商品學硏究 Vol.41 No.1
기존의 한국 예능프로그램 중국 수출에 대한 연구는 주로 한국 오리지널 콘텐츠에 대한 해외 시청자의 인식에 초점을 맞추고 있어 포맷수출을 고려한 현지화 콘텐츠 제작 현황을 비교하는데 한계가 있었다. 본 연구에서는 한국 오리지널 예능, 현지화된 중국 예능, 중국 오리지널 예능 프로그램을 별도로 분리하고 시청자 만족도에 미치는 예능프로그램 포맷 특성에 대하여 분석하였다. 이를 위하여 위의 세 가지 유형의 콘텐츠를 모두 시청한 136명의 중국인을 대상으로 설문조사를 실시하고 시청자 만족도에 미치는 변수를 통제변수와 독립변수로 나누었다. 통제변수는 성별, 연령, 학력, 직업변수로 구성하고 독립변수는 주제특성, 제작특성, 출연진특성을 포함하여 세 가지 유형의 예능콘텐츠 시청만족도에 미치는 영향을 분석하였다. 연구결과에 따르면 한국 오리지널 예능, 중국화된 한국 예능, 중국 오리지널 예능의 시청만족도에 미치는 프로그램 특성요인은 서로 다른 것으로 나타났다. 한국 오리지널 예능이나 중국화된 한국 예능과 같이 한국 콘텐츠를 기반으로 하는 경우 출연진이 모두 중요하였으며, 중국화된 한국 예능이나 중국 오리지널 예능과 같이 중국에서 제작되는 경우 제작기술은 시청만족도에 미치는 영향이 유의하지 않았다. 한국 오리지널 예능 프로그램을 제작할 때는 중국 시청자들이 좋아하는 연예인 및 스타를 중심으로 프로그램을 기획할 필요가 있으며 포맷수출을 통하여 중국화 프로그램을 제작할 때도 출연진 선택에 우선순위를 두는 전략이 필요할 것으로 판단된다.
창상치료용 오적골 혼합 하이드로콜로이드 막의 개발 및 효능
곽문화,김지은,이영주,송성화,고준,이재원,인정훈,김은정,정영진,윤영현,황대연 한국생체재료학회 2013 생체재료학회지 Vol.17 No.2
Cuttlefish bone (CB) which is the hard tissue acting as a floating tank in cuttlefish has application to various fields including a bone substitute, biosensor matrix substrate, antioxidant and scaffold for tissue engineering. Aim of this study was whether hydrocolloid membrane containing cuttlefish bone (HMCB) could contribute to the improvement of the wound repair process in skin of SD rats. To achieve this, HMCB was developed using the mixture dispersed CB powder in hydrocolloid, and an alteration on the histology and physiology of wound skin were observed in SD rats after the treatment of HMCB for 10 days. The dispersion solution of cuttlefish bone showed low salt concentration and electric resistance, high electrical conductivity, and medium pH level. Also, two types of HMCB (HMCB15 and HMCB30) were successfully prepared with adding two different concentrations of cuttlefish bone into hydrocolloid mixture. A significant enhancement of absorptiveness was detected in HMCB30 compared with that of HM or HMCB15, although there was no difference between HM and HMCB15. Furthermore, in animals study, the diameter of wound area was significantly shorter in HMCB30 treated group than that in either HM or HMCB15 treated group. Especially, rats treated with HMCB30 displayed significant improvement in scar tissue reduction, epithelium regeneration, angiogenesis and extracellular matrix deposition in wound area, as compare with those of HM treated group. Therefore, these results indicated that HMCB could be applied therapeutically to aid wound healing in the skin of SD rats. Also, the presented data suggest that HMCB have great potential for the treatment of burn, diabetic and chronic wound defects.
미더덕껍질 혼합 하이드로콜로이드막의 물성분석 및 랫드의 피부창상 치료효능에 관한 연구
곽문화,고준,김지은,이영주,이승현,손홍주,이희섭,정영진,황대연 한국생체재료학회 2013 생체재료학회지 Vol.17 No.3
Aim of this study was to determine the effect of hydrocolloid membrane containing Styela clava tunic (HCM-SCT) on acute wound of skin. To achieve this, HCM-SCT was developed using the mixture dispersed SCT powder in hydrocolloid (HC), and an alteration on the histology and physiology of wound skin were observed in SD rats after the treatment of HCM-SCT for 11 days. Absorptiveness of HCM-SCT was rapidly increased by 430% at day 1 and then their level was maintained for a period of 4 days, while the surface roughness was higher in HCM-SCT than that of HCM.In animals study, SD rats treated with HCM-SCT did not induce any specific toxicity on the body weight, and the physical function of liver and kidney. The diameter of wound area was shorter in HCM-SCT treated group than these in Gauze (GZ) or HCM treated group. Especially, the group treated with HCM-SCT showed a significantly faster re-epithelization and decrease of epidermis thickness than those treated with HCM alone although a large amount of HC was remained in dermis region of both rats. Furthermore, the level of collagen and tumor growth factor-β1 (TGF-β1) were greatly increased in HCM-SCT treated group, while the level of angiogenesis-related proteins were significantly recovered to normal level in the same group. Therefore, these results indicate that HCM-SCT having high absorptiveness and tensile strength could accelerate the repair process of acute wound in SD rats through the stimulation of re-epithelization, collagen deposition and angiogenesis.
고분자필름과 금속막 의료소재에 대한 생체적합성 및 독성 평가를 위한 새로운 세포배양시스템의 개발 및 적용
곽문화(Moon Hwa Kwak),윤우빈(Woo Bin Yun),김지은(Ji Eun Kim),성지은(Ji Eun Sung),이현아(Hyun Ah Lee),서은지(Eun Ji Seo),남국일(Gug Il Nam),정영진(Young Jin Jung),황대연(Dae Youn Hwang) 한국생명과학회 2016 생명과학회지 Vol.26 No.6
고분자(polymer), 금속(metal), 세라믹(ceramic), 합성물(composite) 등과 같은 바이오소소재(Biomaterials)는 그들의 물리화학적 성질 때문에 의료용섬유(medical fibers), 인공혈관(artificial blood vessels), 인공관절(artificial joints), 임플란트(implants), 연조직(soft tissue), 인공성형물(plastic surgery materials) 등 의료용으로 많이 사용되며, 개발연구도 활발히 진행되고 있다. 그러나, 필름(film)이나 판(plate)형태의 바이오소재에 대한 생체적합성(biocompatibility) 이나 독성(toxicity)을 포유동물세포를 이용하여 평가하는 것은 적절한 평가용 장치가 없기 때문에 매우 어려운 상황이다. 따라서 이러한 문제를 해결하기 위해서, 본 연구에서는 고분자필름이나 금속판에 유용하게 적용할 수 있는 실리콘링, 상판(top panel), 하판(bottom panel)으로 구성된 새로운 포유동물배양시스템을 개발하고, 이를 실제 적용하고자 하였다. 개발된 시스템은 평가하고자 하는 시료를 상판과 하판사이에 조립하는 샌드위치시스템을 기반으로 한다. 세포배양장치의 조립 후, SK-MEL-2세포를 3가지 시료; Styela Clava Tunic (SCT)- PF, NaHCO₃-added SCT (SCTN)-PF, magnesium MP (MMP)에 적용하고 37°C 이산화탄소 배양기에서 24시간과 48시간 동안 배양하였다. MTT분석결과에서, 세포생존율(cell viability)은 24시간과 48시간 동안 SCT-PF배양그룹에서 정상적으로 유지되었지만 48시간 동안 SCTN-PF배양그룹에서는 급격하게 감소되었다. 더불어, MMP배양그룹에서 세포생존율은 24시간과 48시간 배양 후에 대조군과 유사하게 유지되었다. 이러한 결과는 본 연구에서 새롭게 개발된 샌드위치형태의 포유동물세포배양장치는 고분자필름이나 금속판형태의 바이오소재에 대한 독성이나 생체적합성을 평가하기 위한 우수한 잠재력을 보유하고 있음을 제시하고 있다. Biomaterials including polymer, metal, ceramic, and composite have been widely applied for medical uses as medical fibers, artificial blood vessels, artificial joints, implants, soft tissue, and plastic surgery materials owing to their physicochemical properties. However, the biocompatibility and toxicity for film- and plate-form biomaterials is difficult to measure in mammalian cells because there is no appropriate incubation system. To solve these problems, we developed a novel mammalian cell culture system consisting of a silicone ring, top panel, and bottom panel and we applied two polymer films (PF) and one metal plate (MP). This system was based on the principal of sandwiching a test sample between the top panel and the bottom panel. Following the assembly of the culture system, SK-MEL-2 cells were seeded onto Styela Clava Tunic (SCT)-PF, NaHCO₃-added SCT (SCTN)-PF, and magnesium MP (MMP) and incubated at 37°C for 24 hr and 48 hr. An MTT assay revealed that cell viability was maintained at a normal level in the SCT-PF culture group at 24 or 48 hr, although it rapidly decreased in the SCTN-PF culture group at 48 hr. Furthermore, the cell viability in the MMP culture group was very similar to that of the control group after incubation for 24 hr and 48 hr. Together, these results suggest the sandwich-type mammalian culture system developed here has the potential for the evaluation of the biocompatibility and toxicity of cells against PF- and MP-form biomaterials.
파지 디스플레이를 이용한 췌장암 특이적 결합 펩타이드 개발
이동원,박재명,양승목,곽문화,노윤진,이인석,최명규 대한소화기학회 2019 대한소화기학회지 Vol.74 No.1
Background/Aims: Pancreatic cancer has a very poor prognosis, and early diagnosis is a way to increase the survival rate of patients. The purpose of this study was to develop pancreatic cancer-specific peptides for imaging studies. Methods: Three pancreatic cancer cell lines, MIA PaCa-2, UACC-462, and BxPC-3, and a control cell line, CCD841, were used. Biopannings were performed on MIA PaCa-2 using a phage display library. After this, the peptides were synthesized and labeled with fluorescein isothiocyanate (FITC). Immunocytochemistry (ICC), enzyme-linked immunosorbent assay (ELISA), and fluorescence- activated cell sorter (FACS) were performed to examine the specific binding. To examine its therapeutic applications, a photosensitizer, chlorin e6 (Ce6), was conjugated on the peptide and photodynamic therapy was performed. Cell survival was investigated using a [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. Results: After three biopannings, the phages were amplified from 1.4×104 to 3.2×105 plaque-forming units. The most strongly binding phage was selected from the ELISA and ICC results. FITC-labeled peptide, M5, in the three pancreatic cancer cell lines showed significantly higher immunofluorescence in the ICC experiments than that of CCD841. The higher binding ability to MIA PaCa-2 cells was confirmed from FACS analysis, which showed a right shift compared to CCD841. M5 bound to Ce6 showed a significantly lower cell survival rate than that of Ce6 alone in photodynamic therapy, which was observed consistently as a change in the tumor size and fluorescence intensity in MIA PaCa-2 cell-implanted animal models. Conclusions: This study showed that the noble peptide, M5, binds specifically to the pancreatic cancer cell line, MIA PaCa-2. The M5 peptide has potential use in future optical diagnostic and therapeutic purposes.